LOCUS       V006123                 9053 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006123
VERSION     V006123
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9053)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9053)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9053)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9053)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             452..472
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             488..829
                     /label="GAL4 activation domain"
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     misc_feature    complement(847..2117)
                     /label="3' UTR of mCASP7"
                     /note="3' UTR of mCASP7"
     CDS             complement(2121..3029)
                     /gene="Casp7"
                     /label="Caspase-7"
                     /note="Caspase-7 from Mus musculus. Accession#: P97864"
     misc_feature    complement(3030..3218)
                     /label="5' UTR of mCASP7"
                     /note="5' UTR of mCASP7"
     terminator      3635..3822
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(3943..5034)
                     /label="LEU2"
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(5035..5439)
                     /label="LEU2 promoter"
     primer_bind     complement(5481..5497)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5505..5521)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5529..5559)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5574..5595)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5883..6471)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6645..7502)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7503..7607)
                     /label="AmpR promoter"
     rep_origin      7889..9053
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa
      421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg
      481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
      541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
      601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
      661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
      721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
      781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattcgg
      841 cacgagtttt tttttttttt tttttttttt ttttctgaaa cacattttaa tcaataaata
      901 ggaacatctg tcaaccattt attaggtggg aaaaacagga atgtataacg aattccaaag
      961 aaaatgatac cttggaaaga aactgctaag atcttcgcct aaattgtcat ttgtagaacc
     1021 agaggtaacc aaagattatt cagaaaaaaa aatcttggaa tatttgaaag aattccagat
     1081 gtatttgaag tacaaaaaca ttatcttgta tacaaagtga aatgggttac tcttcagcac
     1141 ctgtgtggat ggatgatctg acaaatcagt gaatagtgaa tgaatacatt gtagcacgcc
     1201 agaggacatg gttatttaat tcacattttg agccaagttt acccccacca ctacccctgc
     1261 tttgctatcc cttcctctgc tctgtagcac agggtagcat ggtccctagg ccctcactag
     1321 ttggctttca ggcaccttgg aattataagg cgctggtgga tatgggcaca ggcctgggga
     1381 ggcctttggt gtctgactca gttctggctt ggtttccatc tcaggcagtg gcttgtcctg
     1441 agcctggctc caatcaccat agtcctccat ggttctagtc tctagaaggc tgccctgcca
     1501 cagttcttac tgatgacaag ggaaccgaag tcctcttaca gccccacaga ggcatgtgag
     1561 gtaacgggat tgactgtctc tcaattaccc gagtaagtga attccctgta ttgacccttc
     1621 tgcaccgata ttcctaggat ggcttatttt ctggattggg ttctatatac tggaatatta
     1681 actgtcgtct tctctcagcc acctgggaag gtcggcttaa aaatctgccc taagccactg
     1741 agaacaacca ctcagcagcc acctttgcca atggaggcca acaggtgtcg cagctgtatt
     1801 ctttcatcaa gagctgggat tctctttgcc aagttcaact gagacgtcat tgccaatgtc
     1861 ttctccagag gtcatgctca cagcatgcac tcagcgagac gacaaagaaa aaaagaaaca
     1921 gaaacaggaa atcaacctta gaggaagagc atgaagtcat cagatgaaca aagttattaa
     1981 acaaaaaaaa aaaaatctag actttcctga aattaaatcc cctcaacatt tctggatatt
     2041 ttgagagcat cggaacaaaa ataaaataaa aactggattc atcaacgaac ggcggcaggt
     2101 tctcagctga agggtggtca acggctgaag tacagctctt tggtgagcat ggacaccata
     2161 cacgggatct gcttcttctc gttgaagcgt ggatcatcag actgggactc gaagtgcctg
     2221 gccaccctgt cgttcaccct ggtcaggatc tgcatgatct cgaggtcctt gccatgctca
     2281 ttcaggatgg agcagagggc ctgcacaaac caggagcctt tccctgggtt cctccatgag
     2341 taataacctg gaaccgtgga gtaagcaaag aggaagtcgg cttccaccgg gatcttgttg
     2401 cggggattag cgtcaatgtc gttgatgggc cccgagtcag cctggattcc atcatcgagc
     2461 tccgtccctc ggcatgcctg aatgaagaag agtttgggtt tctctaacag ggttttgcat
     2521 cggtctcccc taaaatgagc tgtcagatcc tttatgggtg tcacgccatc tttcccgtaa
     2581 atcaggtcct cttccccgtg gctcagcagg acgcaggcga agcaggccga gttgctgtgg
     2641 tcctcctcag aggcttttct aagcagatct tgcatctttg cacaagagca gtcattgtgg
     2701 acggttactt caaaacccag gttttggaag cacttgaaga gggcccctgc atctttgtcc
     2761 gtcccattcc ggacgtccat acctgtcgct ttgtcgaagt tcttgttgtt tatgatgatg
     2821 catttaccca tcttctggaa atccatgcgg tacagataag tgggcactcg gtcccggccg
     2881 gtcctgacgg ggcccgcaga ggcatttctc ttcttcttca agagaataga ggagatgata
     2941 gaggagcggt ctggcttggc gtcaactccg tcttcgctgg aagaatccac cttttccagc
     3001 tccgcagcac agtcctgatc atcggtcatc gttcccacgg cggcggccag gagcccacgg
     3061 cagcgtccgt cctccgcaag ggtcgagggc agctttggga aaactgaaag caaaagtccc
     3121 ggaggcggag ggggaggagc gggcgggccg gacggggctg tgcctgcagc ccgggaaagc
     3181 tgggggcgag cgggaacgcg cgctacggag cccgccgcct cgtgccgaat tcccggggat
     3241 ccgtcgacct gcagagatct atgaatcgta gatactgaaa aaccccgcaa gttcacttca
     3301 actgtgcatc gtgcaccatc tcaatttctt tcatttatac atcgttttgc cttcttttat
     3361 gtaactatac tcctctaagt ttcaatcttg gccatgtaac ctctgatcta tagaattttt
     3421 taaatgacta gaattaatgc ccatcttttt tttggaccta aattcttcat gaaaatatat
     3481 tacgagggct tattcagaag ctttggactt cttcgccaga ggtttggtca agtctccaat
     3541 caaggttgtc ggcttgtcta ccttgccaga aatttacgaa aagatggaaa agggtcaaat
     3601 cgttggtaga tacgttgttg acacttctaa ataagcgaat ttcttatgat ttatgatttt
     3661 tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt
     3721 tttaaaacga aaattcttgt tcttgagtaa ctctttcctg taggtcaggt tgctttctca
     3781 ggtatagcat gaggtcgctc ttattgacca cacctctacc ggcatgcccg aaattcccct
     3841 accctatgaa catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat
     3901 aaagtttatg tacaaatatc ataaaaaaag agaatctttt taagcaagga ttttcttaac
     3961 ttcttcggcg acagcatcac cgacttcggt ggtactgttg gaaccaccta aatcaccagt
     4021 tctgatacct gcatccaaaa cctttttaac tgcatcttca atggccttac cttcttcagg
     4081 caagttcaat gacaatttca acatcattgc agcagacaag atagtggcga tagggtcaac
     4141 cttattcttt ggcaaatctg gagcagaacc gtggcatggt tcgtacaaac caaatgcggt
     4201 gttcttgtct ggcaaagagg ccaaggacgc agatggcaac aaacccaagg aacctgggat
     4261 aacggaggct tcatcggaga tgatatcacc aaacatgttg ctggtgatta taataccatt
     4321 taggtgggtt gggttcttaa ctaggatcat ggcggcagaa tcaatcaatt gatgttgaac
     4381 cttcaatgta ggaaattcgt tcttgatggt ttcctccaca gtttttctcc ataatcttga
     4441 agaggccaaa acattagctt tatccaagga ccaaataggc aatggtggct catgttgtag
     4501 ggccatgaaa gcggccattc ttgtgattct ttgcacttct ggaacggtgt attgttcact
     4561 atcccaagcg acaccatcac catcgtcttc ctttctctta ccaaagtaaa tacctcccac
     4621 taattctctg acaacaacga agtcagtacc tttagcaaat tgtggcttga ttggagataa
     4681 gtctaaaaga gagtcggatg caaagttaca tggtcttaag ttggcgtaca attgaagttc
     4741 tttacggatt tttagtaaac cttgttcagg tctaacacta cctgtacccc atttaggacc
     4801 acccacagca cctaacaaaa cggcatcaac cttcttggag gcttccagcg cctcatctgg
     4861 aagtgggaca cctgtagcat cgatagcagc accaccaatt aaatgatttt cgaaatcgaa
     4921 cttgacattg gaacgaacat cagaaatagc tttaagaacc ttaatggctt cggctgtgat
     4981 ttcttgacca acgtggtcac ctggcaaaac gacgatcttc ttaggggcag acattagaat
     5041 ggtatatcct tgaaatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa
     5101 agtaagacga ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca
     5161 acttcaagta ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag
     5221 ccggttccgg cctctcacct ttcctttttc tcccaatttt tcagttgaaa aaggtatatg
     5281 cgtcaggcga cctctgaaat taacaaaaaa tttccagtca tcgaatttga ttctgtgcga
     5341 tagcgcccct gtgtgttctc gttatgttga ggaaaaaaat aatggttgct aagagattcg
     5401 aactcttgca tcttacgata cctgagtatt cccacagttg gggatctcga ctctagctag
     5461 aggatcaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     5521 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     5581 aggtaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     5641 tgccagctgg attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5701 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5761 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5821 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5881 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5941 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6001 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6061 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6121 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6181 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6241 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6301 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     6361 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6421 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6481 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6541 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6601 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6661 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6721 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6781 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6841 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6901 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6961 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7021 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7081 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7141 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7201 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7261 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7321 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7381 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7441 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7501 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7561 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7621 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     7681 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     7741 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     7801 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     7861 gagcagattg tactgagagt gcaccataac gcatttaagc ataaacacgc actatgccgt
     7921 tcttctcatg tatatatata tacaggcaac acgcagatat aggtgcgacg tgaacagtga
     7981 gctgtatgtg cgcagctcgc gttgcatttt cggaagcgct cgttttcgga aacgctttga
     8041 agttcctatt ccgaagttcc tattctctag ctagaaagta taggaacttc agagcgcttt
     8101 tgaaaaccaa aagcgctctg aagacgcact ttcaaaaaac caaaaacgca ccggactgta
     8161 acgagctact aaaatattgc gaataccgct tccacaaaca ttgctcaaaa gtatctcttt
     8221 gctatatatc tctgtgctat atccctatat aacctaccca tccacctttc gctccttgaa
     8281 cttgcatcta aactcgacct ctacattttt tatgtttatc tctagtatta ctctttagac
     8341 aaaaaaattg tagtaagaac tattcataga gtgaatcgaa aacaatacga aaatgtaaac
     8401 atttcctata cgtagtatat agagacaaaa tagaagaaac cgttcataat tttctgacca
     8461 atgaagaatc atcaacgcta tcactttctg ttcacaaagt atgcgcaatc cacatcggta
     8521 tagaatataa tcggggatgc ctttatcttg aaaaaatgca cccgcagctt cgctagtaat
     8581 cagtaaacgc gggaagtgga gtcaggcttt ttttatggaa gagaaaatag acaccaaagt
     8641 agccttcttc taaccttaac ggacctacag tgcaaaaagt tatcaagaga ctgcattata
     8701 gagcgcacaa aggagaaaaa aagtaatcta agatgctttg ttagaaaaat agcgctctcg
     8761 ggatgcattt ttgtagaaca aaaaagaagt atagattctt tgttggtaaa atagcgctct
     8821 cgcgttgcat ttctgttctg taaaaatgca gctcagattc tttgtttgaa aaattagcgc
     8881 tctcgcgttg catttttgtt ttacaaaaat gaagcacaga ttcttcgttg gtaaaatagc
     8941 gctttcgcgt tgcatttctg ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt
     9001 agcgctctcg cgttgcattt ttgttctaca aaatgaagca cagatgcttc gtt
//
