LOCUS       V006124                 7910 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006124
VERSION     V006124
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7910)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7910)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7910)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             452..472
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             488..829
                     /label="GAL4 activation domain"
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     misc_feature    complement(847..1220)
                     /label="3UTR"
                     /note="3UTR"
     CDS             complement(1224..2051)
                     /gene="Casp6"
                     /label="Caspase-6"
                     /note="Caspase-6 from Mus musculus. Accession#: O08738"
     misc_feature    complement(2052..2075)
                     /label="5UTR"
                     /note="5UTR"
     terminator      2492..2679
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(2800..3891)
                     /label="LEU2"
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(3892..4296)
                     /label="LEU2 promoter"
     primer_bind     complement(4338..4354)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4362..4378)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4386..4416)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4431..4452)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4740..5328)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5502..6359)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6360..6464)
                     /label="AmpR promoter"
     rep_origin      6746..7910
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa
      421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg
      481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
      541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
      601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
      661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
      721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
      781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattcgg
      841 cacgaggttc ttagacattt tattacgcga aaacaatctc agtctagagt ttcaaaagtc
      901 tttacaaaca ttatacagta aactcatata aacagtgtca caaaagggga ggcaaagtag
      961 ttggtattta gagtaatctt acagtttact aaaaatgtaa taaatgtagt agacattgat
     1021 gcatagtaat ttcctgagga ttatttttag cccttccacc acgtccaaca tgattgaaaa
     1081 gtaacaaata taaaacatta cttttctatg gcatctacaa aattgaagca gtttacattg
     1141 atgggtgaaa gtcttccacc agctggcctt tgtataaaaa gggaaatgtg tgtgaaatat
     1201 atgtagcaag acagatggcc ctacttgcta ggtttgggac agaaatgcag ctttttggtc
     1261 agcattgagg cgaagcaggg cacctgcttc ttgccgattg catccgggtc tttgcagaag
     1321 tccacgcggc gctgagagac ctttctgttc accagcgtga gcagctccgt gaactccagg
     1381 gaactgccgt acctcgccag catctcacac aaatcctgaa tgtaccagga gccattcaca
     1441 gtttctcggt gagagtaata cccttctgcg acagagtagc acatgaggaa gtctgcccct
     1501 gccggcagcg tgtacacgga cgcagcatcc acctgggtca cgttgtccag cttgtctgtc
     1561 tggtgatcca ccatgtccag gggaaccacg ggtacgtcat gctggctacc ccgacaggcc
     1621 tggatgataa atatcttggg ttttccaacc aggctctgac acttgtctcc tttgaacaag
     1681 ccagtcaacg tctgaatttc aattttggcg tcgtatgcgt aaacgtggtt gccttcccca
     1741 tggctcagga agacacagat gaagcaatcg gcatctatgt ggcttgaagt cgacacctcg
     1801 tgaattttga gcaggagttc ttctgctctg aggtcgttaa agcatttcac ttcaaatcct
     1861 agatctgaaa acctgcgagt caggttgtct ctgtctgcgt tggtgccccg cctctctggg
     1921 agggtcaggt gccaaaagaa cctctcgtga ttgaagatga gggcaactcc tctcctcttg
     1981 tggtccatct tgtactgctc ggctggatcg aacacttccc tacttttgta gaagccatcg
     2041 gtttctgtca tgttttactt tcacctgccc gcgagctcgt gccgaattcc cggggatccg
     2101 tcgacctgca gagatctatg aatcgtagat actgaaaaac cccgcaagtt cacttcaact
     2161 gtgcatcgtg caccatctca atttctttca tttatacatc gttttgcctt cttttatgta
     2221 actatactcc tctaagtttc aatcttggcc atgtaacctc tgatctatag aattttttaa
     2281 atgactagaa ttaatgccca tctttttttt ggacctaaat tcttcatgaa aatatattac
     2341 gagggcttat tcagaagctt tggacttctt cgccagaggt ttggtcaagt ctccaatcaa
     2401 ggttgtcggc ttgtctacct tgccagaaat ttacgaaaag atggaaaagg gtcaaatcgt
     2461 tggtagatac gttgttgaca cttctaaata agcgaatttc ttatgattta tgatttttat
     2521 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
     2581 aaaacgaaaa ttcttgttct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
     2641 atagcatgag gtcgctctta ttgaccacac ctctaccggc atgcccgaaa ttcccctacc
     2701 ctatgaacat attccatttt gtaatttcgt gtcgtttcta ttatgaattt catttataaa
     2761 gtttatgtac aaatatcata aaaaaagaga atctttttaa gcaaggattt tcttaacttc
     2821 ttcggcgaca gcatcaccga cttcggtggt actgttggaa ccacctaaat caccagttct
     2881 gatacctgca tccaaaacct ttttaactgc atcttcaatg gccttacctt cttcaggcaa
     2941 gttcaatgac aatttcaaca tcattgcagc agacaagata gtggcgatag ggtcaacctt
     3001 attctttggc aaatctggag cagaaccgtg gcatggttcg tacaaaccaa atgcggtgtt
     3061 cttgtctggc aaagaggcca aggacgcaga tggcaacaaa cccaaggaac ctgggataac
     3121 ggaggcttca tcggagatga tatcaccaaa catgttgctg gtgattataa taccatttag
     3181 gtgggttggg ttcttaacta ggatcatggc ggcagaatca atcaattgat gttgaacctt
     3241 caatgtagga aattcgttct tgatggtttc ctccacagtt tttctccata atcttgaaga
     3301 ggccaaaaca ttagctttat ccaaggacca aataggcaat ggtggctcat gttgtagggc
     3361 catgaaagcg gccattcttg tgattctttg cacttctgga acggtgtatt gttcactatc
     3421 ccaagcgaca ccatcaccat cgtcttcctt tctcttacca aagtaaatac ctcccactaa
     3481 ttctctgaca acaacgaagt cagtaccttt agcaaattgt ggcttgattg gagataagtc
     3541 taaaagagag tcggatgcaa agttacatgg tcttaagttg gcgtacaatt gaagttcttt
     3601 acggattttt agtaaacctt gttcaggtct aacactacct gtaccccatt taggaccacc
     3661 cacagcacct aacaaaacgg catcaacctt cttggaggct tccagcgcct catctggaag
     3721 tgggacacct gtagcatcga tagcagcacc accaattaaa tgattttcga aatcgaactt
     3781 gacattggaa cgaacatcag aaatagcttt aagaacctta atggcttcgg ctgtgatttc
     3841 ttgaccaacg tggtcacctg gcaaaacgac gatcttctta ggggcagaca ttagaatggt
     3901 atatccttga aatatatata tatattgctg aaatgtaaaa ggtaagaaaa gttagaaagt
     3961 aagacgattg ctaaccacct attggaaaaa acaataggtc cttaaataat attgtcaact
     4021 tcaagtattg tgatgcaagc atttagtcat gaacgcttct ctattctata tgaaaagccg
     4081 gttccggcct ctcacctttc ctttttctcc caatttttca gttgaaaaag gtatatgcgt
     4141 caggcgacct ctgaaattaa caaaaaattt ccagtcatcg aatttgattc tgtgcgatag
     4201 cgcccctgtg tgttctcgtt atgttgagga aaaaaataat ggttgctaag agattcgaac
     4261 tcttgcatct tacgatacct gagtattccc acagttgggg atctcgactc tagctagagg
     4321 atcaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     4381 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagg
     4441 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     4501 cagctggatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     4561 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4621 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4681 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4741 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4801 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4861 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4921 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4981 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5041 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5101 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5161 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5221 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5281 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5341 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5401 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     5461 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     5521 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     5581 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     5641 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     5701 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     5761 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     5821 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     5881 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5941 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6001 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6061 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6121 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     6181 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     6241 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     6301 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     6361 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6421 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6481 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     6541 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     6601 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     6661 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     6721 cagattgtac tgagagtgca ccataacgca tttaagcata aacacgcact atgccgttct
     6781 tctcatgtat atatatatac aggcaacacg cagatatagg tgcgacgtga acagtgagct
     6841 gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt tttcggaaac gctttgaagt
     6901 tcctattccg aagttcctat tctctagcta gaaagtatag gaacttcaga gcgcttttga
     6961 aaaccaaaag cgctctgaag acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg
     7021 agctactaaa atattgcgaa taccgcttcc acaaacattg ctcaaaagta tctctttgct
     7081 atatatctct gtgctatatc cctatataac ctacccatcc acctttcgct ccttgaactt
     7141 gcatctaaac tcgacctcta cattttttat gtttatctct agtattactc tttagacaaa
     7201 aaaattgtag taagaactat tcatagagtg aatcgaaaac aatacgaaaa tgtaaacatt
     7261 tcctatacgt agtatataga gacaaaatag aagaaaccgt tcataatttt ctgaccaatg
     7321 aagaatcatc aacgctatca ctttctgttc acaaagtatg cgcaatccac atcggtatag
     7381 aatataatcg gggatgcctt tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag
     7441 taaacgcggg aagtggagtc aggctttttt tatggaagag aaaatagaca ccaaagtagc
     7501 cttcttctaa ccttaacgga cctacagtgc aaaaagttat caagagactg cattatagag
     7561 cgcacaaagg agaaaaaaag taatctaaga tgctttgtta gaaaaatagc gctctcggga
     7621 tgcatttttg tagaacaaaa aagaagtata gattctttgt tggtaaaata gcgctctcgc
     7681 gttgcatttc tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa ttagcgctct
     7741 cgcgttgcat ttttgtttta caaaaatgaa gcacagattc ttcgttggta aaatagcgct
     7801 ttcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc
     7861 gctctcgcgt tgcatttttg ttctacaaaa tgaagcacag atgcttcgtt
//
