LOCUS       V006125                 7978 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006125
VERSION     V006125
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7978)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7978)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7978)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             452..472
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             488..829
                     /label="GAL4 activation domain"
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     misc_feature    complement(847..1242)
                     /label="3UTR"
                     /note="3UTR"
     CDS             complement(1246..2076)
                     /gene="Casp3"
                     /label="Caspase-3"
                     /note="Caspase-3 from Mus musculus. Accession#: P70677"
     misc_feature    complement(2077..2143)
                     /label="5UTR"
                     /note="5UTR"
     terminator      2560..2747
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(2868..3959)
                     /label="LEU2"
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(3960..4364)
                     /label="LEU2 promoter"
     primer_bind     complement(4406..4422)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4430..4446)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4454..4484)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4499..4520)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4808..5396)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5570..6427)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6428..6532)
                     /label="AmpR promoter"
     rep_origin      6814..7978
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa
      421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg
      481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
      541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
      601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
      661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
      721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
      781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattcgg
      841 cacgagggta gttgaaatga aatttaattt aataaggagc aagtaactgt caatgatggt
      901 actatcatcc tagatgaaat tacaaagttg cccttttata attagcaaaa ttcatagtat
      961 cgccaaatct tgctaattat aaaagggcaa ctttgtaatt tcatctagga tgatagtacc
     1021 atcattgaca gttacttgct ccttattaaa ttaaatttca tttcaactac ctgatgtcga
     1081 agttgaggta gctgcactgt ggctggctgc attgctaggc agtggtagcg tacaaagacc
     1141 aggaggaccg tcagattaga tttaaatgag agtctccctg acagctttct catttggcat
     1201 aacaaaacag aaacacgccc ccccccaccc ccaatcattc ctctagtgat aaaagtacag
     1261 ttctttcgtg agcatggaca caatacacgg gatctgtttc tttgcgtgga aagtggagtc
     1321 cagggagaag gactcgaatt ccgttgccac cttcctgtta acgcgagtga gaatgtgcat
     1381 aaattctagc ttgtgcgcgt acagcttcag catgctgcaa agggactgga tgaaccacga
     1441 cccgtccttt gaatttctcc aggaatagta accaggtgct gtagagtaag catacaggaa
     1501 gtcagcctcc accggtatct tctggcaagc catctcctca tcagtcccac tgtctgtctc
     1561 aatgccacag tccagctccg taccccggca ggcctgaatg atgaagagtt tcggctttcc
     1621 agtcagactc cggcagtagt cgcctctgaa gaagctagtc aactttttca gttcaacagg
     1681 cccatttgtc ccataaatga ccccttcatc accatggctt agaatcacac acacaaagct
     1741 gctccttttg ctatgatctt ccttagaaac actatccatt aattccaaaa tgtcttcacg
     1801 agtaagatca tttttattcc tgacttggta tttcaggccc atgaatgtct ctctgaggtt
     1861 ggctgcgtcc acatccgtac cagagcgaga tgacattcca gtgctcttat ggaagttctt
     1921 attattaatt attatgcata tgcccatttc aggataatcc attttgtaac tactgtccag
     1981 atagatccca gagtccactg acttgctccc atgtatggtc tttacttcaa aattattaat
     2041 ggattttgaa tccactgagg ttttgttgtt ctccatggtc acttttctta gcgtaccgtt
     2101 ccaagctccc cgccgcagga cactgcgctt tgatcccacc agcctcgtgc cgaattcccg
     2161 gggatccgtc gacctgcaga gatctatgaa tcgtagatac tgaaaaaccc cgcaagttca
     2221 cttcaactgt gcatcgtgca ccatctcaat ttctttcatt tatacatcgt tttgccttct
     2281 tttatgtaac tatactcctc taagtttcaa tcttggccat gtaacctctg atctatagaa
     2341 ttttttaaat gactagaatt aatgcccatc ttttttttgg acctaaattc ttcatgaaaa
     2401 tatattacga gggcttattc agaagctttg gacttcttcg ccagaggttt ggtcaagtct
     2461 ccaatcaagg ttgtcggctt gtctaccttg ccagaaattt acgaaaagat ggaaaagggt
     2521 caaatcgttg gtagatacgt tgttgacact tctaaataag cgaatttctt atgatttatg
     2581 atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct
     2641 taggttttaa aacgaaaatt cttgttcttg agtaactctt tcctgtaggt caggttgctt
     2701 tctcaggtat agcatgaggt cgctcttatt gaccacacct ctaccggcat gcccgaaatt
     2761 cccctaccct atgaacatat tccattttgt aatttcgtgt cgtttctatt atgaatttca
     2821 tttataaagt ttatgtacaa atatcataaa aaaagagaat ctttttaagc aaggattttc
     2881 ttaacttctt cggcgacagc atcaccgact tcggtggtac tgttggaacc acctaaatca
     2941 ccagttctga tacctgcatc caaaaccttt ttaactgcat cttcaatggc cttaccttct
     3001 tcaggcaagt tcaatgacaa tttcaacatc attgcagcag acaagatagt ggcgataggg
     3061 tcaaccttat tctttggcaa atctggagca gaaccgtggc atggttcgta caaaccaaat
     3121 gcggtgttct tgtctggcaa agaggccaag gacgcagatg gcaacaaacc caaggaacct
     3181 gggataacgg aggcttcatc ggagatgata tcaccaaaca tgttgctggt gattataata
     3241 ccatttaggt gggttgggtt cttaactagg atcatggcgg cagaatcaat caattgatgt
     3301 tgaaccttca atgtaggaaa ttcgttcttg atggtttcct ccacagtttt tctccataat
     3361 cttgaagagg ccaaaacatt agctttatcc aaggaccaaa taggcaatgg tggctcatgt
     3421 tgtagggcca tgaaagcggc cattcttgtg attctttgca cttctggaac ggtgtattgt
     3481 tcactatccc aagcgacacc atcaccatcg tcttcctttc tcttaccaaa gtaaatacct
     3541 cccactaatt ctctgacaac aacgaagtca gtacctttag caaattgtgg cttgattgga
     3601 gataagtcta aaagagagtc ggatgcaaag ttacatggtc ttaagttggc gtacaattga
     3661 agttctttac ggatttttag taaaccttgt tcaggtctaa cactacctgt accccattta
     3721 ggaccaccca cagcacctaa caaaacggca tcaaccttct tggaggcttc cagcgcctca
     3781 tctggaagtg ggacacctgt agcatcgata gcagcaccac caattaaatg attttcgaaa
     3841 tcgaacttga cattggaacg aacatcagaa atagctttaa gaaccttaat ggcttcggct
     3901 gtgatttctt gaccaacgtg gtcacctggc aaaacgacga tcttcttagg ggcagacatt
     3961 agaatggtat atccttgaaa tatatatata tattgctgaa atgtaaaagg taagaaaagt
     4021 tagaaagtaa gacgattgct aaccacctat tggaaaaaac aataggtcct taaataatat
     4081 tgtcaacttc aagtattgtg atgcaagcat ttagtcatga acgcttctct attctatatg
     4141 aaaagccggt tccggcctct cacctttcct ttttctccca atttttcagt tgaaaaaggt
     4201 atatgcgtca ggcgacctct gaaattaaca aaaaatttcc agtcatcgaa tttgattctg
     4261 tgcgatagcg cccctgtgtg ttctcgttat gttgaggaaa aaaataatgg ttgctaagag
     4321 attcgaactc ttgcatctta cgatacctga gtattcccac agttggggat ctcgactcta
     4381 gctagaggat caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4441 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4501 gagtgaggta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4561 tgtcgtgcca gctggattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4621 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     4681 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4741 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4801 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4861 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4921 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4981 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5041 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5101 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5161 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5221 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     5281 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5341 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5401 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5461 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5521 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5581 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5641 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5701 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     5761 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     5821 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     5881 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5941 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6001 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6061 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6121 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6181 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6241 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6301 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6361 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6421 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6481 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6541 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     6601 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     6661 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     6721 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     6781 catcagagca gattgtactg agagtgcacc ataacgcatt taagcataaa cacgcactat
     6841 gccgttcttc tcatgtatat atatatacag gcaacacgca gatataggtg cgacgtgaac
     6901 agtgagctgt atgtgcgcag ctcgcgttgc attttcggaa gcgctcgttt tcggaaacgc
     6961 tttgaagttc ctattccgaa gttcctattc tctagctaga aagtatagga acttcagagc
     7021 gcttttgaaa accaaaagcg ctctgaagac gcactttcaa aaaaccaaaa acgcaccgga
     7081 ctgtaacgag ctactaaaat attgcgaata ccgcttccac aaacattgct caaaagtatc
     7141 tctttgctat atatctctgt gctatatccc tatataacct acccatccac ctttcgctcc
     7201 ttgaacttgc atctaaactc gacctctaca ttttttatgt ttatctctag tattactctt
     7261 tagacaaaaa aattgtagta agaactattc atagagtgaa tcgaaaacaa tacgaaaatg
     7321 taaacatttc ctatacgtag tatatagaga caaaatagaa gaaaccgttc ataattttct
     7381 gaccaatgaa gaatcatcaa cgctatcact ttctgttcac aaagtatgcg caatccacat
     7441 cggtatagaa tataatcggg gatgccttta tcttgaaaaa atgcacccgc agcttcgcta
     7501 gtaatcagta aacgcgggaa gtggagtcag gcttttttta tggaagagaa aatagacacc
     7561 aaagtagcct tcttctaacc ttaacggacc tacagtgcaa aaagttatca agagactgca
     7621 ttatagagcg cacaaaggag aaaaaaagta atctaagatg ctttgttaga aaaatagcgc
     7681 tctcgggatg catttttgta gaacaaaaaa gaagtataga ttctttgttg gtaaaatagc
     7741 gctctcgcgt tgcatttctg ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt
     7801 agcgctctcg cgttgcattt ttgttttaca aaaatgaagc acagattctt cgttggtaaa
     7861 atagcgcttt cgcgttgcat ttctgttctg taaaaatgca gctcagattc tttgtttgaa
     7921 aaattagcgc tctcgcgttg catttttgtt ctacaaaatg aagcacagat gcttcgtt
//
