LOCUS       V006126                10145 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006126
VERSION     V006126
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10145)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10145)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10145)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             452..472
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             488..829
                     /label="GAL4 activation domain"
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     misc_feature    complement(847..2865)
                     /label="3UTR"
                     /note="3UTR"
     CDS             complement(2869..4224)
                     /gene="Casp2"
                     /label="Caspase-2"
                     /note="Caspase-2 from Mus musculus. Accession#: P29594"
     misc_feature    complement(4225..4309)
                     /label="5UTR"
                     /note="5UTR"
     terminator      4727..4914
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(5035..6126)
                     /label="LEU2"
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(6127..6531)
                     /label="LEU2 promoter"
     primer_bind     complement(6573..6589)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    6597..6613
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6621..6651)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6666..6687)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6975..7563)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7737..8594)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8595..8699)
                     /label="AmpR promoter"
     rep_origin      8981..10145
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      9165..10145
                     /label="2mu ori"
                     /note="2mu ori"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa
      421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg
      481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
      541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
      601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
      661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
      721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
      781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattcgg
      841 cacgagatca acatttattt ggcacctgat ggcaatacaa aatcttggca gtgggaagtg
      901 tgaagattct accacttatg catcctatac tgacccaaaa ggcagaattt ggcagcaagt
      961 cttcaaacca cttggttcat acaacaaaca ttagttgagc acctcaacta ccataggcct
     1021 caaatttgct gggcactatg gagatgtgaa agaagttgtc tcacacgtga tatgtagaaa
     1081 atataaaaaa acaacaacaa aacagtggat aaaaataaaa cacctggaaa aaaaaaccca
     1141 tatactaatt gaactgtaca ctataatagc aatttagaaa ctgcaaaagg taggctctaa
     1201 ctaaggttag ctgctggtct ccttccttta ctcaggaaac attcatgaag acctagtaag
     1261 tgccagttac tggatatcag taagtaagaa gcacagttcc cactctcttg gagcattcag
     1321 cctcagaggg aacaagtgaa acaagcaaaa gtgccattac agcctgcagt aagcacaaca
     1381 gagaccaaca tgatgccctc ataaggaggc acagtagaca gcatgggcag catggtcaag
     1441 aggcctccgc cagactcaaa aatcagagac tagccaaagg agagttcaga acacctgcaa
     1501 gacggagatg aggacctaag aggggctgga gcatagtgag aaatcaccat ttaatgagat
     1561 ccaaagggaa ggtatgagcc agatctcgta agctgggtgt aagaatttgc attttagatg
     1621 aagcgtaaca ggaagctagt gttctaagca agagaatgac atcaccagat attttaaaaa
     1681 ccaaactact ctgttacatg gagaataaat tggaagagag gaaaaaatgg aaatgcagaa
     1741 ggttttcaag aataagcaag tttcataaga gcagagacaa gtgcttctag gttatatcac
     1801 atatggtact atccccacta aaacacagag aaacaatgag aagggagacg ctgccacaga
     1861 aaaccaaaga agcctcacgc tgaagcagaa ggcagaaaga ataagcaact acagtacagc
     1921 gtaagtggca agaaacatcc tacaaaggac ggcttcacag acttggcaga agcagcgaat
     1981 ggtgaaggta aagaggggaa ccagtacaaa gcaagagaga ccctggaggc cagccgtcaa
     2041 gtacagtgta agtgcatgca gaagccacag taaggaagga gagacatcct gatgacgggt
     2101 gatagtgtga gacacagcac actcagctgt cactgcattt tcatgtagtt atgcccgata
     2161 aggttcattc agtgacagtt caaacaaagc atgaccaggg aaatttctat gacttccagt
     2221 gtcatggcat acagacccta ggctctgttc aaaggctggg tgcactgaac acaaaggaac
     2281 caatggaaag gctgagggac tgtccttcac ctagggcctg aacttaaagg tatgcataga
     2341 aacttcgcaa gtagtaggat ctcatctgtc ccttcatgac agatgaaaac acctacatta
     2401 aattgggaca tcttccaaat ctccactgtg ggaaaaatga ggttgtagat atggaaaaaa
     2461 aaggagatga tctctgagac tccttaactg atttaggaaa aaaaaaaaaa gctatgatcc
     2521 aagtactcct aagatgcctt gggaacaaaa atatcacaaa cattgagaaa tccaaatgcc
     2581 ctcttcccac aataatactg tgttcacaat gtcattttct aggctgtagc taaaatgctt
     2641 ctggtaacac atccattcag agctggctct ataggtagag aacacgacac cggaggagcc
     2701 atccttatgt ttcataccca aagacagaga tggctgacag gaaacaagcc tgggagaatc
     2761 tcatatccct gagagggtag acagaacaaa aaccattctg aagagaagtc tccattgtgc
     2821 ccccagtggt ccagtggcct ccaacagcag gaatagcagg cggcatcacg tgggtgggta
     2881 gcctgggaac aggtagagtt gctggcacag agtactacag tactcagaca tctccttgca
     2941 tcggtggaat tctgtgccag gggcatagcc ttcacgctcc ttgataaggg cattcacctt
     3001 aacaagcatg tcggccacgt gcatgtcaca agctctttca gagaacacct gagtgagggc
     3061 ctcaatgtac caggaacccc gtttggtgtt ccgcatggca gcattacctt taaggcaagc
     3121 atagccacat atcatgtctg agcgagtagg cagtctcatc ttcatcaact cctctttgcc
     3181 agcatcactc tcctcacatc caggggattg tgtgtggttc tttccatctt gctggtcgac
     3241 acctctatct gtctcatctc cacgacatgc ttggatgaag aacatttttg gcttgttctg
     3301 tagacttgga cagttagcat tgtcaaaaag tcgaaaaacc tcttggagct gaagcagttt
     3361 gccatctaca ccatagatgc caccttccac accatgtgag aggagtgcca ctacgcagga
     3421 gtctgtgacc cggtgtgcag gtaactgtgc aaaattctga agtttctctt gcatttcctg
     3481 tgcggtctgg tcatgtagca catggacatt gtagcccaaa agcttgaaga gggtgactag
     3541 agtagtgtgg tccacatccc ctccagagcg gaattccagg tctttctctc cagtgaagtg
     3601 cacattgctc agcaccagtg ccaagccacg gggctgagat tgcaacctat aggccagctg
     3661 gtagtgtgcc tggtaaaact caggagtgca tggcttcacc agaagacagg gaggaccatc
     3721 accattatct aaggagtgtt ccgtagcatc tgtggatagg cggagctgct tgtgaggagg
     3781 gcaggactca cacaccgaga aagggagact tgtgtcatag tcacagctca acggtgggag
     3841 tacgtgctga atatctgaga gggttgtgag cagtaagtct tccaagtgac cctgcctggt
     3901 ctcccgcagg gcttcacaga aggcatcaaa agcctggggt cctctctttg gcagcaggtt
     3961 gaggagttcc acattctggc tgaaactgcc ccctttggcc tggatgagct ccctcatttc
     4021 caaagtgata atgtccttct ctaggaggtg ttctaacagc tcgctcagca gcagctgctt
     4081 ggccagcacc actcgattct ttttcagtgt ttcctggtgg tcaggatgca ttccacacac
     4141 tgccaaaatc ctgctcctcc tgtcagccgc catcagcccc ttcctgtgca gggaggactg
     4201 cgacctcccg ctcggcgccg ccatttccag cttttcccgg gctgcacacg caccacccac
     4261 aaaacaaaca aacgctgggc cgcgccagcc cccgcatccc tcccccagcc ctcgtgccga
     4321 attcccgggg atccgtcgac ctgcagagat ctatgaatcg tagatactga aaaaccccgc
     4381 aagttcactt caactgtgca tcgtgcacca tctcaatttc tttcatttat acatcgtttt
     4441 gccttctttt atgtaactat actcctctaa gtttcaatct tggccatgta acctctgatc
     4501 tatagaattt tttaaatgac tagaattaat gcccatcttt tttttggacc taaattcttc
     4561 atgaaaatat attacgaggg cttattcaga agctttggac ttcttcgcca gaggtttggt
     4621 caagtctcca atcaaggttg tcggcttgtc taccttgcca gaaatttacg aaaagatgga
     4681 aaagggtcaa atcgttggta gatacgttgt tgacacttct aaataagcga atttcttatg
     4741 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
     4801 tgactcttag gttttaaaac gaaaattctt gttcttgagt aactctttcc tgtaggtcag
     4861 gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgcc
     4921 cgaaattccc ctaccctatg aacatattcc attttgtaat ttcgtgtcgt ttctattatg
     4981 aatttcattt ataaagttta tgtacaaata tcataaaaaa agagaatctt tttaagcaag
     5041 gattttctta acttcttcgg cgacagcatc accgacttcg gtggtactgt tggaaccacc
     5101 taaatcacca gttctgatac ctgcatccaa aaccttttta actgcatctt caatggcctt
     5161 accttcttca ggcaagttca atgacaattt caacatcatt gcagcagaca agatagtggc
     5221 gatagggtca accttattct ttggcaaatc tggagcagaa ccgtggcatg gttcgtacaa
     5281 accaaatgcg gtgttcttgt ctggcaaaga ggccaaggac gcagatggca acaaacccaa
     5341 ggaacctggg ataacggagg cttcatcgga gatgatatca ccaaacatgt tgctggtgat
     5401 tataatacca tttaggtggg ttgggttctt aactaggatc atggcggcag aatcaatcaa
     5461 ttgatgttga accttcaatg taggaaattc gttcttgatg gtttcctcca cagtttttct
     5521 ccataatctt gaagaggcca aaacattagc tttatccaag gaccaaatag gcaatggtgg
     5581 ctcatgttgt agggccatga aagcggccat tcttgtgatt ctttgcactt ctggaacggt
     5641 gtattgttca ctatcccaag cgacaccatc accatcgtct tcctttctct taccaaagta
     5701 aatacctccc actaattctc tgacaacaac gaagtcagta cctttagcaa attgtggctt
     5761 gattggagat aagtctaaaa gagagtcgga tgcaaagtta catggtctta agttggcgta
     5821 caattgaagt tctttacgga tttttagtaa accttgttca ggtctaacac tacctgtacc
     5881 ccatttagga ccacccacag cacctaacaa aacggcatca accttcttgg aggcttccag
     5941 cgcctcatct ggaagtggga cacctgtagc atcgatagca gcaccaccaa ttaaatgatt
     6001 ttcgaaatcg aacttgacat tggaacgaac atcagaaata gctttaagaa ccttaatggc
     6061 ttcggctgtg atttcttgac caacgtggtc acctggcaaa acgacgatct tcttaggggc
     6121 agacattaga atggtatatc cttgaaatat atatatatat tgctgaaatg taaaaggtaa
     6181 gaaaagttag aaagtaagac gattgctaac cacctattgg aaaaaacaat aggtccttaa
     6241 ataatattgt caacttcaag tattgtgatg caagcattta gtcatgaacg cttctctatt
     6301 ctatatgaaa agccggttcc ggcctctcac ctttcctttt tctcccaatt tttcagttga
     6361 aaaaggtata tgcgtcaggc gacctctgaa attaacaaaa aatttccagt catcgaattt
     6421 gattctgtgc gatagcgccc ctgtgtgttc tcgttatgtt gaggaaaaaa ataatggttg
     6481 ctaagagatt cgaactcttg catcttacga tacctgagta ttcccacagt tggggatctc
     6541 gactctagct agaggatcaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6601 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6661 gcctaatgag tgaggtaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6721 ggaaacctgt cgtgccagct ggattaatga atcggccaac gcgcggggag aggcggtttg
     6781 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6841 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6901 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6961 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7021 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7081 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7141 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7201 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7261 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7321 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7381 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     7441 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7501 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7561 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7621 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7681 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7741 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7801 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7861 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7921 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7981 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8041 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8101 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8161 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8221 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8281 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8341 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8401 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8461 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8521 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8581 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8641 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8701 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     8761 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     8821 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     8881 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     8941 tatgcggcat cagagcagat tgtactgaga gtgcaccata acgcatttaa gcataaacac
     9001 gcactatgcc gttcttctca tgtatatata tatacaggca acacgcagat ataggtgcga
     9061 cgtgaacagt gagctgtatg tgcgcagctc gcgttgcatt ttcggaagcg ctcgttttcg
     9121 gaaacgcttt gaagttccta ttccgaagtt cctattctct agctagaaag tataggaact
     9181 tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa accaaaaacg
     9241 caccggactg taacgagcta ctaaaatatt gcgaataccg cttccacaaa cattgctcaa
     9301 aagtatctct ttgctatata tctctgtgct atatccctat ataacctacc catccacctt
     9361 tcgctccttg aacttgcatc taaactcgac ctctacattt tttatgttta tctctagtat
     9421 tactctttag acaaaaaaat tgtagtaaga actattcata gagtgaatcg aaaacaatac
     9481 gaaaatgtaa acatttccta tacgtagtat atagagacaa aatagaagaa accgttcata
     9541 attttctgac caatgaagaa tcatcaacgc tatcactttc tgttcacaaa gtatgcgcaa
     9601 tccacatcgg tatagaatat aatcggggat gcctttatct tgaaaaaatg cacccgcagc
     9661 ttcgctagta atcagtaaac gcgggaagtg gagtcaggct ttttttatgg aagagaaaat
     9721 agacaccaaa gtagccttct tctaacctta acggacctac agtgcaaaaa gttatcaaga
     9781 gactgcatta tagagcgcac aaaggagaaa aaaagtaatc taagatgctt tgttagaaaa
     9841 atagcgctct cgggatgcat ttttgtagaa caaaaaagaa gtatagattc tttgttggta
     9901 aaatagcgct ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg
     9961 aaaaattagc gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca gattcttcgt
    10021 tggtaaaata gcgctttcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt
    10081 gtttgaaaaa ttagcgctct cgcgttgcat ttttgttcta caaaatgaag cacagatgct
    10141 tcgtt
//
