LOCUS       V006127                 8908 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006127
VERSION     V006127
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8908)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8908)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             452..472
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             488..829
                     /label="GAL4 activation domain"
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     misc_feature    complement(847..1780)
                     /label="3UTR"
                     /note="3UTR"
     CDS             complement(1784..3040)
                     /gene="Casp12"
                     /label="Caspase-12"
                     /note="Caspase-12 from Mus musculus. Accession#: O08736"
     misc_feature    complement(3041..3073)
                     /label="5UTR"
                     /note="5UTR"
     terminator      3490..3677
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(3798..4889)
                     /label="LEU2"
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(4890..5294)
                     /label="LEU2 promoter"
     primer_bind     complement(5336..5352)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5360..5376)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5384..5414)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5429..5450)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5738..6326)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6500..7357)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7358..7462)
                     /label="AmpR promoter"
     rep_origin      7744..8908
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa
      421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg
      481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
      541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
      601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
      661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
      721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
      781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattcgg
      841 cacgagtttt tttttttttt tttttataga acaaagaata aacaaagatt ttaatttgag
      901 ctcagttaag taaaaataat gatataacat cctcaagatg acaaaaatag tggtaggtgg
      961 gcttcatcac atacttgtag aagaattcaa ttttgcatag aatttcatag aatattttgt
     1021 ctcaaaattg tctttaatgc tttaaacttt taatttttgt ttctaggatt tatttcaata
     1081 acactaatag acagaatctt aaatgtgtat gtgtgagtat atcttattac aaaggcattt
     1141 aagagaaaaa ttataaacat agcataaatt tgtcaattat atcctaagaa attgattgaa
     1201 aggaatggta ggtagccact aggcatgaaa accatgtatg ccagagacaa acagtggaca
     1261 taaacctctt tatcttattc ctaaaagatg attagtttta cctacatatg caacagtttc
     1321 atttctcagc tacattgtag ataagatctt ccttgtgagt aaattcttac tactaattta
     1381 taggtgaaag tggttcatca aaaggtgtat catctttgtt ctttagtgtc tttttccttt
     1441 attcatttta caaagaagaa gttccaggat atgttcagac atgttcttta gtgtcttttt
     1501 cctttattca ttttacaaaa atgtgcaaat ataaatttaa taaaataaag taaaagatga
     1561 gcagatttgg aaggaaggaa cgaatctata cttttcatgc aaaattttac gtatcaggtt
     1621 tcccctaaaa ataacctatg gaaattatct agccttatga tattgtataa aatatctaac
     1681 tgaaaagttt tacacattta aaaatcctgg gatcttggaa ttttcttttc ttctcagcta
     1741 cagcaatact tgactgggaa ctgcatgaga gttgcctgtg ctaattcccg ggaaaaaggt
     1801 agaaatagcg tgtcatggat actctctcaa tagtgggcat ctgggtcagt tcacctggga
     1861 cctcaaatga gtgttgaacc tttcgaaaaa tttcctccaa atgataacac caacagtact
     1921 ttttgaagca gtcaatgagt ttggaaatga agagggaacc agtcttgcct accttccaag
     1981 aaatattatg tggggtagaa gatttgaaag caatgaaatc tgtctccaca tgggcctttg
     2041 ttatactatt attccattta cagctcaaca cacgttcctc atctgtatca gcagtggcta
     2101 tccctttgtt tgtggatacc caaatagttc cattatatct gcctctgcag gcctgcatga
     2161 tgagaatctt gggtttgttt ctcagactcc gacagttaga gttgttgaaa attttgaaga
     2221 tagtgtcatc atgaagaaca tctggctttt tgtttcggtg cttcacccca cagattcctt
     2281 ccaggatgcc atgggacata aacaccagga atgtgctgtc tgaggactgg tgctctggac
     2341 ggccagcaaa ctgcattaac tctgtctcca tttcctgagc tgtaaggttt tcctttaaca
     2401 ccacagagta tccaagattt tcaagtagtt cttgcatgtt caaaatgtca gtatcagcat
     2461 tatctctatc aaaaaggtag tcaaactttt tgttgcagat gatgagagcc agacgtgttc
     2521 gtccctcctt ctccatcact ggatatatct cttttgccct ttctgtcttt atcttacaaa
     2581 actgatcacg tggacaaagc ttcagtgtat cttggacttc tgagctctgg agtccatgag
     2641 gagctgtcag cattagatgt gattcatggg ccaatccagc atttacctcc atttcatcat
     2701 cctctacttt tctcttggat tctgatggag aagaaggtgt acatatcttc tgaggtccat
     2761 catcctcatc attagaaaat tgtaaactca gctgttcctg ggaattggca atgtggccag
     2821 caaatatttt tcctgccatg tctgttttct ctaagaagtt ctcaaccaga ttctcagcct
     2881 tgttcaggat gaaactcgca ctttccccta ttttgagtaa ctcatctcca tttaaaacat
     2941 tcttctccac caggtcatca aaaaccccat ccagcatgtc cttggccaaa cctttgatct
     3001 tgtagattgg atctctttca tgtgtcctcc tggccgccat ggctgtgctc gtgccgctcg
     3061 tgccgctcgt gccctcgtgc cgaattcccg gggatccgtc gacctgcaga gatctatgaa
     3121 tcgtagatac tgaaaaaccc cgcaagttca cttcaactgt gcatcgtgca ccatctcaat
     3181 ttctttcatt tatacatcgt tttgccttct tttatgtaac tatactcctc taagtttcaa
     3241 tcttggccat gtaacctctg atctatagaa ttttttaaat gactagaatt aatgcccatc
     3301 ttttttttgg acctaaattc ttcatgaaaa tatattacga gggcttattc agaagctttg
     3361 gacttcttcg ccagaggttt ggtcaagtct ccaatcaagg ttgtcggctt gtctaccttg
     3421 ccagaaattt acgaaaagat ggaaaagggt caaatcgttg gtagatacgt tgttgacact
     3481 tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa
     3541 ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt cttgttcttg
     3601 agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt
     3661 gaccacacct ctaccggcat gcccgaaatt cccctaccct atgaacatat tccattttgt
     3721 aatttcgtgt cgtttctatt atgaatttca tttataaagt ttatgtacaa atatcataaa
     3781 aaaagagaat ctttttaagc aaggattttc ttaacttctt cggcgacagc atcaccgact
     3841 tcggtggtac tgttggaacc acctaaatca ccagttctga tacctgcatc caaaaccttt
     3901 ttaactgcat cttcaatggc cttaccttct tcaggcaagt tcaatgacaa tttcaacatc
     3961 attgcagcag acaagatagt ggcgataggg tcaaccttat tctttggcaa atctggagca
     4021 gaaccgtggc atggttcgta caaaccaaat gcggtgttct tgtctggcaa agaggccaag
     4081 gacgcagatg gcaacaaacc caaggaacct gggataacgg aggcttcatc ggagatgata
     4141 tcaccaaaca tgttgctggt gattataata ccatttaggt gggttgggtt cttaactagg
     4201 atcatggcgg cagaatcaat caattgatgt tgaaccttca atgtaggaaa ttcgttcttg
     4261 atggtttcct ccacagtttt tctccataat cttgaagagg ccaaaacatt agctttatcc
     4321 aaggaccaaa taggcaatgg tggctcatgt tgtagggcca tgaaagcggc cattcttgtg
     4381 attctttgca cttctggaac ggtgtattgt tcactatccc aagcgacacc atcaccatcg
     4441 tcttcctttc tcttaccaaa gtaaatacct cccactaatt ctctgacaac aacgaagtca
     4501 gtacctttag caaattgtgg cttgattgga gataagtcta aaagagagtc ggatgcaaag
     4561 ttacatggtc ttaagttggc gtacaattga agttctttac ggatttttag taaaccttgt
     4621 tcaggtctaa cactacctgt accccattta ggaccaccca cagcacctaa caaaacggca
     4681 tcaaccttct tggaggcttc cagcgcctca tctggaagtg ggacacctgt agcatcgata
     4741 gcagcaccac caattaaatg attttcgaaa tcgaacttga cattggaacg aacatcagaa
     4801 atagctttaa gaaccttaat ggcttcggct gtgatttctt gaccaacgtg gtcacctggc
     4861 aaaacgacga tcttcttagg ggcagacatt agaatggtat atccttgaaa tatatatata
     4921 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat
     4981 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat
     5041 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct
     5101 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca
     5161 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat
     5221 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga
     5281 gtattcccac agttggggat ctcgactcta gctagaggat caattcgtaa tcatggtcat
     5341 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5401 gcataaagtg taaagcctgg ggtgcctaat gagtgaggta actcacatta attgcgttgc
     5461 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctggattaa tgaatcggcc
     5521 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5581 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5641 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5701 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5761 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5821 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5881 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5941 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6001 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6061 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6121 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6181 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6241 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6301 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6361 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6421 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6481 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6541 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6601 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6661 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6721 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6781 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6841 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6901 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6961 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7021 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7081 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7141 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7201 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7261 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7321 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7381 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7441 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     7501 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     7561 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     7621 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     7681 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     7741 ataacgcatt taagcataaa cacgcactat gccgttcttc tcatgtatat atatatacag
     7801 gcaacacgca gatataggtg cgacgtgaac agtgagctgt atgtgcgcag ctcgcgttgc
     7861 attttcggaa gcgctcgttt tcggaaacgc tttgaagttc ctattccgaa gttcctattc
     7921 tctagctaga aagtatagga acttcagagc gcttttgaaa accaaaagcg ctctgaagac
     7981 gcactttcaa aaaaccaaaa acgcaccgga ctgtaacgag ctactaaaat attgcgaata
     8041 ccgcttccac aaacattgct caaaagtatc tctttgctat atatctctgt gctatatccc
     8101 tatataacct acccatccac ctttcgctcc ttgaacttgc atctaaactc gacctctaca
     8161 ttttttatgt ttatctctag tattactctt tagacaaaaa aattgtagta agaactattc
     8221 atagagtgaa tcgaaaacaa tacgaaaatg taaacatttc ctatacgtag tatatagaga
     8281 caaaatagaa gaaaccgttc ataattttct gaccaatgaa gaatcatcaa cgctatcact
     8341 ttctgttcac aaagtatgcg caatccacat cggtatagaa tataatcggg gatgccttta
     8401 tcttgaaaaa atgcacccgc agcttcgcta gtaatcagta aacgcgggaa gtggagtcag
     8461 gcttttttta tggaagagaa aatagacacc aaagtagcct tcttctaacc ttaacggacc
     8521 tacagtgcaa aaagttatca agagactgca ttatagagcg cacaaaggag aaaaaaagta
     8581 atctaagatg ctttgttaga aaaatagcgc tctcgggatg catttttgta gaacaaaaaa
     8641 gaagtataga ttctttgttg gtaaaatagc gctctcgcgt tgcatttctg ttctgtaaaa
     8701 atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt ttgttttaca
     8761 aaaatgaagc acagattctt cgttggtaaa atagcgcttt cgcgttgcat ttctgttctg
     8821 taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg catttttgtt
     8881 ctacaaaatg aagcacagat gcttcgtt
//
