LOCUS       V006128                 8026 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006128
VERSION     V006128
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8026)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8026)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8026)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             452..472
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             488..829
                     /label="GAL4 activation domain"
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     misc_feature    complement(847..1041)
                     /label="3' UTR of mCASP11"
                     /note="3' UTR of mCASP11"
     CDS             complement(1045..2163)
                     /gene="Casp4"
                     /label="Caspase-4"
                     /note="Caspase-4 from Mus musculus. Accession#: P70343"
     misc_feature    complement(2164..2189)
                     /label="5' UTR of mCASP11"
                     /note="5' UTR of mCASP11"
     terminator      2608..2795
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(2916..4007)
                     /label="LEU2"
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(4008..4412)
                     /label="LEU2 promoter"
     primer_bind     complement(4454..4470)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4478..4494)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4502..4532)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4547..4568)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4856..5444)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5618..6475)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6476..6580)
                     /label="AmpR promoter"
     rep_origin      6862..8026
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa
      421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg
      481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
      541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
      601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
      661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
      721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
      781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattcgg
      841 cacgagtttt tttttttttt tgggcttgtt tgcttgtcta tggaacagaa tcccactgtg
      901 ttgcttaggc tggtcttgaa ttcataagta ttctttcctt aatttcacta gttctggaat
      961 ttcaggaata gaatgtgatc cttggccaag atcacttctt caagctgaag aaatgagatt
     1021 cagttgcttg ttgctttgtt ctcagttgcc aggaaagagg tagaaatatc tcgtcaaggt
     1081 tgcccgatca atggtgggca tctgggaatg aatacttgcc ttttcaaatg attgttgcac
     1141 cttcaggaat atatcaaaga gatgacaaga gcaagcatgt ttccggaagc aggaaatgag
     1201 tctagtgatg aagtaagagc ctcctgtttt gtctcggtag gacaagtgat gtggggttgt
     1261 agagtagaag gcaatgaagt ccttctccac gtggctcagc ttgacagcat cagcttccat
     1321 attcctaggt agatctacac ctctgcacaa ctggggtttt gaagactctc tgatccacat
     1381 ttctccagag ttcccacctc tgcaggcctg cacaatgatg actttgggtt tgtctcgtag
     1441 acctggacag tggcaattgt tgaatatctg atagatggta tcatactgta gcacatctgg
     1501 agttttttca ctgtgcattg ttccacaaat gccatgcagt gtgccatgag acattagcac
     1561 caggaatgtg ctgtctgatg tctggtgttc tgagagtgca gcaaagtctt tcatctctga
     1621 ctccatgccc tctgctgtaa gctcctcttt caccaccaca tcgtagccta agtcttcaag
     1681 aaggcctttc ataccaatga tgtcaaagtt agccccatac ctcagtgaga gatgtttgaa
     1741 ctctgtattg catatgataa gagcctttcg tgtacggcca ttggcctcct ttattgggta
     1801 aatttcttgt gtcttttctc tgcaaagcct tgtgaactct tcaggggaac aaagcttgag
     1861 agtgttcaat gattcttctg gttcctccat ttccagatta gcttcaccat ggtggctgcc
     1921 tgggtccaca ctgaagaatg tctggagaag catttcacct actttgctgt gtttcttttt
     1981 catggcatct acaaaaaccc aacgcttgtc actgcgttca gcattgttaa atttttgttt
     2041 atcttcctcc tttaatttca gtacattgct ttgtactaat ttttctaggt actccgtaag
     2101 gacttctttg cccagctgtt ccaacacctt aagtggtttg tcagggtgtt tgttttcagc
     2161 catgagaaaa agcctcagtt ctttcgcacc gctcgtgccg aattcccggg gatccgtcga
     2221 cctgcagaga tctatgaatc gtagatactg aaaaaccccg caagttcact tcaactgtgc
     2281 atcgtgcacc atctcaattt ctttcattta tacatcgttt tgccttcttt tatgtaacta
     2341 tactcctcta agtttcaatc ttggccatgt aacctctgat ctatagaatt ttttaaatga
     2401 ctagaattaa tgcccatctt ttttttggac ctaaattctt catgaaaata tattacgagg
     2461 gcttattcag aagctttgga cttcttcgcc agaggtttgg tcaagtctcc aatcaaggtt
     2521 gtcggcttgt ctaccttgcc agaaatttac gaaaagatgg aaaagggtca aatcgttggt
     2581 agatacgttg ttgacacttc taaataagcg aatttcttat gatttatgat ttttattatt
     2641 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa
     2701 cgaaaattct tgttcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag
     2761 catgaggtcg ctcttattga ccacacctct accggcatgc ccgaaattcc cctaccctat
     2821 gaacatattc cattttgtaa tttcgtgtcg tttctattat gaatttcatt tataaagttt
     2881 atgtacaaat atcataaaaa aagagaatct ttttaagcaa ggattttctt aacttcttcg
     2941 gcgacagcat caccgacttc ggtggtactg ttggaaccac ctaaatcacc agttctgata
     3001 cctgcatcca aaaccttttt aactgcatct tcaatggcct taccttcttc aggcaagttc
     3061 aatgacaatt tcaacatcat tgcagcagac aagatagtgg cgatagggtc aaccttattc
     3121 tttggcaaat ctggagcaga accgtggcat ggttcgtaca aaccaaatgc ggtgttcttg
     3181 tctggcaaag aggccaagga cgcagatggc aacaaaccca aggaacctgg gataacggag
     3241 gcttcatcgg agatgatatc accaaacatg ttgctggtga ttataatacc atttaggtgg
     3301 gttgggttct taactaggat catggcggca gaatcaatca attgatgttg aaccttcaat
     3361 gtaggaaatt cgttcttgat ggtttcctcc acagtttttc tccataatct tgaagaggcc
     3421 aaaacattag ctttatccaa ggaccaaata ggcaatggtg gctcatgttg tagggccatg
     3481 aaagcggcca ttcttgtgat tctttgcact tctggaacgg tgtattgttc actatcccaa
     3541 gcgacaccat caccatcgtc ttcctttctc ttaccaaagt aaatacctcc cactaattct
     3601 ctgacaacaa cgaagtcagt acctttagca aattgtggct tgattggaga taagtctaaa
     3661 agagagtcgg atgcaaagtt acatggtctt aagttggcgt acaattgaag ttctttacgg
     3721 atttttagta aaccttgttc aggtctaaca ctacctgtac cccatttagg accacccaca
     3781 gcacctaaca aaacggcatc aaccttcttg gaggcttcca gcgcctcatc tggaagtggg
     3841 acacctgtag catcgatagc agcaccacca attaaatgat tttcgaaatc gaacttgaca
     3901 ttggaacgaa catcagaaat agctttaaga accttaatgg cttcggctgt gatttcttga
     3961 ccaacgtggt cacctggcaa aacgacgatc ttcttagggg cagacattag aatggtatat
     4021 ccttgaaata tatatatata ttgctgaaat gtaaaaggta agaaaagtta gaaagtaaga
     4081 cgattgctaa ccacctattg gaaaaaacaa taggtcctta aataatattg tcaacttcaa
     4141 gtattgtgat gcaagcattt agtcatgaac gcttctctat tctatatgaa aagccggttc
     4201 cggcctctca cctttccttt ttctcccaat ttttcagttg aaaaaggtat atgcgtcagg
     4261 cgacctctga aattaacaaa aaatttccag tcatcgaatt tgattctgtg cgatagcgcc
     4321 cctgtgtgtt ctcgttatgt tgaggaaaaa aataatggtt gctaagagat tcgaactctt
     4381 gcatcttacg atacctgagt attcccacag ttggggatct cgactctagc tagaggatca
     4441 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4501 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgaggtaac
     4561 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     4621 tggattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     4681 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4741 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4801 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4861 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4921 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4981 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5041 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5101 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5161 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5221 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5281 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5341 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5401 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5461 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5521 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5581 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5641 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5701 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5761 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5821 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5881 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5941 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6001 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6061 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6121 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6181 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     6241 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     6301 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6361 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6421 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6481 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6541 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6601 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     6661 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     6721 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     6781 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     6841 ttgtactgag agtgcaccat aacgcattta agcataaaca cgcactatgc cgttcttctc
     6901 atgtatatat atatacaggc aacacgcaga tataggtgcg acgtgaacag tgagctgtat
     6961 gtgcgcagct cgcgttgcat tttcggaagc gctcgttttc ggaaacgctt tgaagttcct
     7021 attccgaagt tcctattctc tagctagaaa gtataggaac ttcagagcgc ttttgaaaac
     7081 caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac gcaccggact gtaacgagct
     7141 actaaaatat tgcgaatacc gcttccacaa acattgctca aaagtatctc tttgctatat
     7201 atctctgtgc tatatcccta tataacctac ccatccacct ttcgctcctt gaacttgcat
     7261 ctaaactcga cctctacatt ttttatgttt atctctagta ttactcttta gacaaaaaaa
     7321 ttgtagtaag aactattcat agagtgaatc gaaaacaata cgaaaatgta aacatttcct
     7381 atacgtagta tatagagaca aaatagaaga aaccgttcat aattttctga ccaatgaaga
     7441 atcatcaacg ctatcacttt ctgttcacaa agtatgcgca atccacatcg gtatagaata
     7501 taatcgggga tgcctttatc ttgaaaaaat gcacccgcag cttcgctagt aatcagtaaa
     7561 cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa tagacaccaa agtagccttc
     7621 ttctaacctt aacggaccta cagtgcaaaa agttatcaag agactgcatt atagagcgca
     7681 caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa aatagcgctc tcgggatgca
     7741 tttttgtaga acaaaaaaga agtatagatt ctttgttggt aaaatagcgc tctcgcgttg
     7801 catttctgtt ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg
     7861 ttgcattttt gttttacaaa aatgaagcac agattcttcg ttggtaaaat agcgctttcg
     7921 cgttgcattt ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa attagcgctc
     7981 tcgcgttgca tttttgttct acaaaatgaa gcacagatgc ttcgtt
//
