LOCUS       pGAD-C3.        6666 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast two-hybrid """"prey"""" vector for fusing a gene to the GAL4 
            activation domain. For other reading frames, use pGAD-C1 or pGAD-C2.
ACCESSION   .
VERSION     .
KEYWORDS    pGAD-C3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   James P, Halladay J, Craig EA.
  TITLE     Genomic libraries and a host strain designed for highly efficient 
            two-hybrid selection in yeast.
  JOURNAL   Genetics 1996;144:1425-36.
  PUBMED    8978031
REFERENCE   2  (bases 1 to 6666)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6666)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "1996"; volume: "144"; pages: "1425-36"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             452..472
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             488..829
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     misc_feature    833..873
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      1248..1435
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(1556..2647)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(2648..3052)
                     /label=LEU2 promoter
     primer_bind     complement(3094..3110)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3118..3134)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3142..3172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3187..3208)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3496..4084)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4258..5115)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5116..5220)
                     /label=AmpR promoter
     rep_origin      5502..6666
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa
      421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg
      481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
      541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
      601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
      661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
      721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
      781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattccc
      841 gggggatcca tcgatgtcga cctgcagaga tctatgaatc gtagatactg aaaaaccccg
      901 caagttcact tcaactgtgc atcgtgcacc atctcaattt ctttcattta tacatcgttt
      961 tgccttcttt tatgtaacta tactcctcta agtttcaatc ttggccatgt aacctctgat
     1021 ctatagaatt ttttaaatga ctagaattaa tgcccatctt ttttttggac ctaaattctt
     1081 catgaaaata tattacgagg gcttattcag aagctttgga cttcttcgcc agaggtttgg
     1141 tcaagtctcc aatcaaggtt gtcggcttgt ctaccttgcc agaaatttac gaaaagatgg
     1201 aaaagggtca aatcgttggt agatacgttg ttgacacttc taaataagcg aatttcttat
     1261 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     1321 gtgactctta ggttttaaaa cgaaaattct tgttcttgag taactctttc ctgtaggtca
     1381 ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc
     1441 ccgaaattcc cctaccctat gaacatattc cattttgtaa tttcgtgtcg tttctattat
     1501 gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct ttttaagcaa
     1561 ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg ttggaaccac
     1621 ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct tcaatggcct
     1681 taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac aagatagtgg
     1741 cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat ggttcgtaca
     1801 aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc aacaaaccca
     1861 aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg ttgctggtga
     1921 ttataatacc atttaggtgg gttgggttct taactaggat catggcggca gaatcaatca
     1981 attgatgttg aaccttcaat gtaggaaatt cgttcttgat ggtttcctcc acagtttttc
     2041 tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata ggcaatggtg
     2101 gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact tctggaacgg
     2161 tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc ttaccaaagt
     2221 aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca aattgtggct
     2281 tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt aagttggcgt
     2341 acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca ctacctgtac
     2401 cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg gaggcttcca
     2461 gcgcctcatc tggaagtggg acacctgtag cttcgaaagc agcaccacca attaaatgat
     2521 tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga accttaatgg
     2581 cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc ttcttagggg
     2641 cagacattag aatggtatat ccttgaaata tatatatata ttgctgaaat gtaaaaggta
     2701 agaaaagtta gaaagtaaga cgattgctaa ccacctattg gaaaaaacaa taggtcctta
     2761 aataatattg tcaacttcaa gtattgtgat gcaagcattt agtcatgaac gcttctctat
     2821 tctatatgaa aagccggttc cggcctctca cctttccttt ttctcccaat ttttcagttg
     2881 aaaaaggtat atgcgtcagg cgacctctga aattaacaaa aaatttccag tcatcgaatt
     2941 tgattctgtg cgatagcgcc cctgtgtgtt ctcgttatgt tgaggaaaaa aataatggtt
     3001 gctaagagat tcgaactctt gcatcttacg atacctgagt attcccacag ttggggatct
     3061 cgactctagc tagaggatca attcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     3121 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     3181 tgcctaatga gtgaggtaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     3241 gggaaacctg tcgtgccagc tggattaatg aatcggccaa cgcgcgggga gaggcggttt
     3301 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3361 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3421 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3481 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3541 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3601 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3661 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3721 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3781 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3841 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3901 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     3961 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4021 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4081 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4141 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4201 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4261 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4321 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4381 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4441 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4501 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4561 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4621 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4681 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4741 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4801 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4861 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4921 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4981 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5041 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5101 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5161 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     5221 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     5281 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     5341 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     5401 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     5461 ctatgcggca tcagagcaga ttgtactgag agtgcaccat aacgcattta agcataaaca
     5521 cgcactatgc cgttcttctc atgtatatat atatacaggc aacacgcaga tataggtgcg
     5581 acgtgaacag tgagctgtat gtgcgcagct cgcgttgcat tttcggaagc gctcgttttc
     5641 ggaaacgctt tgaagttcct attccgaagt tcctattctc tagctagaaa gtataggaac
     5701 ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac
     5761 gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca
     5821 aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct
     5881 ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt atctctagta
     5941 ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc gaaaacaata
     6001 cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga aaccgttcat
     6061 aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa agtatgcgca
     6121 atccacatcg gtatagaata taatcgggga tgcctttatc ttgaaaaaat gcacccgcag
     6181 cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa
     6241 tagacaccaa agtagccttc ttctaacctt aacggaccta cagtgcaaaa agttatcaag
     6301 agactgcatt atagagcgca caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa
     6361 aatagcgctc tcgggatgca tttttgtaga acaaaaaaga agtatagatt ctttgttggt
     6421 aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt
     6481 gaaaaattag cgctctcgcg ttgcattttt gttttacaaa aatgaagcac agattcttcg
     6541 ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt
     6601 tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa gcacagatgc
     6661 ttcgtt
//
