LOCUS       pGAD-C2.        6665 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast two-hybrid """"prey"""" vector for fusing a gene to the GAL4 
            activation domain. For other reading frames, use pGAD-C1 or pGAD-C3.
ACCESSION   .
VERSION     .
KEYWORDS    pGAD-C2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   James P, Halladay J, Craig EA.
  TITLE     Genomic libraries and a host strain designed for highly efficient 
            two-hybrid selection in yeast.
  JOURNAL   Genetics 1996;144:1425-36.
  PUBMED    8978031
REFERENCE   2  (bases 1 to 6665)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6665)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "1996"; volume: "144"; pages: "1425-36"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             452..472
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             488..829
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     misc_feature    833..872
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      1247..1434
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(1555..2646)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(2647..3051)
                     /label=LEU2 promoter
     primer_bind     complement(3093..3109)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3117..3133)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3141..3171)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3186..3207)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3495..4083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4257..5114)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5115..5219)
                     /label=AmpR promoter
     rep_origin      5501..6665
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa
      421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg
      481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
      541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
      601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
      661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
      721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
      781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattccc
      841 ggggatccat cgatgtcgac ctgcagagat ctatgaatcg tagatactga aaaaccccgc
      901 aagttcactt caactgtgca tcgtgcacca tctcaatttc tttcatttat acatcgtttt
      961 gccttctttt atgtaactat actcctctaa gtttcaatct tggccatgta acctctgatc
     1021 tatagaattt tttaaatgac tagaattaat gcccatcttt tttttggacc taaattcttc
     1081 atgaaaatat attacgaggg cttattcaga agctttggac ttcttcgcca gaggtttggt
     1141 caagtctcca atcaaggttg tcggcttgtc taccttgcca gaaatttacg aaaagatgga
     1201 aaagggtcaa atcgttggta gatacgttgt tgacacttct aaataagcga atttcttatg
     1261 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
     1321 tgactcttag gttttaaaac gaaaattctt gttcttgagt aactctttcc tgtaggtcag
     1381 gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgcc
     1441 cgaaattccc ctaccctatg aacatattcc attttgtaat ttcgtgtcgt ttctattatg
     1501 aatttcattt ataaagttta tgtacaaata tcataaaaaa agagaatctt tttaagcaag
     1561 gattttctta acttcttcgg cgacagcatc accgacttcg gtggtactgt tggaaccacc
     1621 taaatcacca gttctgatac ctgcatccaa aaccttttta actgcatctt caatggcctt
     1681 accttcttca ggcaagttca atgacaattt caacatcatt gcagcagaca agatagtggc
     1741 gatagggtca accttattct ttggcaaatc tggagcagaa ccgtggcatg gttcgtacaa
     1801 accaaatgcg gtgttcttgt ctggcaaaga ggccaaggac gcagatggca acaaacccaa
     1861 ggaacctggg ataacggagg cttcatcgga gatgatatca ccaaacatgt tgctggtgat
     1921 tataatacca tttaggtggg ttgggttctt aactaggatc atggcggcag aatcaatcaa
     1981 ttgatgttga accttcaatg taggaaattc gttcttgatg gtttcctcca cagtttttct
     2041 ccataatctt gaagaggcca aaacattagc tttatccaag gaccaaatag gcaatggtgg
     2101 ctcatgttgt agggccatga aagcggccat tcttgtgatt ctttgcactt ctggaacggt
     2161 gtattgttca ctatcccaag cgacaccatc accatcgtct tcctttctct taccaaagta
     2221 aatacctccc actaattctc tgacaacaac gaagtcagta cctttagcaa attgtggctt
     2281 gattggagat aagtctaaaa gagagtcgga tgcaaagtta catggtctta agttggcgta
     2341 caattgaagt tctttacgga tttttagtaa accttgttca ggtctaacac tacctgtacc
     2401 ccatttagga ccacccacag cacctaacaa aacggcatca accttcttgg aggcttccag
     2461 cgcctcatct ggaagtggga cacctgtagc ttcgaaagca gcaccaccaa ttaaatgatt
     2521 ttcgaaatcg aacttgacat tggaacgaac atcagaaata gctttaagaa ccttaatggc
     2581 ttcggctgtg atttcttgac caacgtggtc acctggcaaa acgacgatct tcttaggggc
     2641 agacattaga atggtatatc cttgaaatat atatatatat tgctgaaatg taaaaggtaa
     2701 gaaaagttag aaagtaagac gattgctaac cacctattgg aaaaaacaat aggtccttaa
     2761 ataatattgt caacttcaag tattgtgatg caagcattta gtcatgaacg cttctctatt
     2821 ctatatgaaa agccggttcc ggcctctcac ctttcctttt tctcccaatt tttcagttga
     2881 aaaaggtata tgcgtcaggc gacctctgaa attaacaaaa aatttccagt catcgaattt
     2941 gattctgtgc gatagcgccc ctgtgtgttc tcgttatgtt gaggaaaaaa ataatggttg
     3001 ctaagagatt cgaactcttg catcttacga tacctgagta ttcccacagt tggggatctc
     3061 gactctagct agaggatcaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3121 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3181 gcctaatgag tgaggtaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3241 ggaaacctgt cgtgccagct ggattaatga atcggccaac gcgcggggag aggcggtttg
     3301 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3361 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3421 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3481 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3541 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3601 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3661 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3721 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3781 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3841 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3901 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     3961 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4021 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4081 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4141 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4201 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4261 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4321 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4381 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4441 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4501 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4561 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4621 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     4681 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     4741 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     4801 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4861 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4921 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4981 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5041 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5101 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5161 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5221 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     5281 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     5341 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     5401 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     5461 tatgcggcat cagagcagat tgtactgaga gtgcaccata acgcatttaa gcataaacac
     5521 gcactatgcc gttcttctca tgtatatata tatacaggca acacgcagat ataggtgcga
     5581 cgtgaacagt gagctgtatg tgcgcagctc gcgttgcatt ttcggaagcg ctcgttttcg
     5641 gaaacgcttt gaagttccta ttccgaagtt cctattctct agctagaaag tataggaact
     5701 tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa accaaaaacg
     5761 caccggactg taacgagcta ctaaaatatt gcgaataccg cttccacaaa cattgctcaa
     5821 aagtatctct ttgctatata tctctgtgct atatccctat ataacctacc catccacctt
     5881 tcgctccttg aacttgcatc taaactcgac ctctacattt tttatgttta tctctagtat
     5941 tactctttag acaaaaaaat tgtagtaaga actattcata gagtgaatcg aaaacaatac
     6001 gaaaatgtaa acatttccta tacgtagtat atagagacaa aatagaagaa accgttcata
     6061 attttctgac caatgaagaa tcatcaacgc tatcactttc tgttcacaaa gtatgcgcaa
     6121 tccacatcgg tatagaatat aatcggggat gcctttatct tgaaaaaatg cacccgcagc
     6181 ttcgctagta atcagtaaac gcgggaagtg gagtcaggct ttttttatgg aagagaaaat
     6241 agacaccaaa gtagccttct tctaacctta acggacctac agtgcaaaaa gttatcaaga
     6301 gactgcatta tagagcgcac aaaggagaaa aaaagtaatc taagatgctt tgttagaaaa
     6361 atagcgctct cgggatgcat ttttgtagaa caaaaaagaa gtatagattc tttgttggta
     6421 aaatagcgct ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg
     6481 aaaaattagc gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca gattcttcgt
     6541 tggtaaaata gcgctttcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt
     6601 gtttgaaaaa ttagcgctct cgcgttgcat ttttgttcta caaaatgaag cacagatgct
     6661 tcgtt
//
