LOCUS       pGAD-C1.        6667 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast two-hybrid """"prey"""" vector for fusing a gene to the GAL4 
            activation domain. For other reading frames, use pGAD-C2 or pGAD-C3.
ACCESSION   .
VERSION     .
KEYWORDS    pGAD-C1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   James P, Halladay J, Craig EA.
  TITLE     Genomic libraries and a host strain designed for highly efficient 
            two-hybrid selection in yeast.
  JOURNAL   Genetics 1996;144:1425-36.
  PUBMED    8978031
REFERENCE   2  (bases 1 to 6667)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6667)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "1996"; volume: "144"; pages: "1425-36"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             452..472
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             488..829
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     misc_feature    833..874
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      1249..1436
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(1557..2648)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(2649..3053)
                     /label=LEU2 promoter
     primer_bind     complement(3095..3111)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3119..3135)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3143..3173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3188..3209)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3497..4085)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4259..5116)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5117..5221)
                     /label=AmpR promoter
     rep_origin      5503..6667
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa
      421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg
      481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
      541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
      601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
      661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
      721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
      781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattccc
      841 cgggggatcc atcgatgtcg acctgcagag atctatgaat cgtagatact gaaaaacccc
      901 gcaagttcac ttcaactgtg catcgtgcac catctcaatt tctttcattt atacatcgtt
      961 ttgccttctt ttatgtaact atactcctct aagtttcaat cttggccatg taacctctga
     1021 tctatagaat tttttaaatg actagaatta atgcccatct tttttttgga cctaaattct
     1081 tcatgaaaat atattacgag ggcttattca gaagctttgg acttcttcgc cagaggtttg
     1141 gtcaagtctc caatcaaggt tgtcggcttg tctaccttgc cagaaattta cgaaaagatg
     1201 gaaaagggtc aaatcgttgg tagatacgtt gttgacactt ctaaataagc gaatttctta
     1261 tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa
     1321 agtgactctt aggttttaaa acgaaaattc ttgttcttga gtaactcttt cctgtaggtc
     1381 aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc taccggcatg
     1441 cccgaaattc ccctacccta tgaacatatt ccattttgta atttcgtgtc gtttctatta
     1501 tgaatttcat ttataaagtt tatgtacaaa tatcataaaa aaagagaatc tttttaagca
     1561 aggattttct taacttcttc ggcgacagca tcaccgactt cggtggtact gttggaacca
     1621 cctaaatcac cagttctgat acctgcatcc aaaacctttt taactgcatc ttcaatggcc
     1681 ttaccttctt caggcaagtt caatgacaat ttcaacatca ttgcagcaga caagatagtg
     1741 gcgatagggt caaccttatt ctttggcaaa tctggagcag aaccgtggca tggttcgtac
     1801 aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg acgcagatgg caacaaaccc
     1861 aaggaacctg ggataacgga ggcttcatcg gagatgatat caccaaacat gttgctggtg
     1921 attataatac catttaggtg ggttgggttc ttaactagga tcatggcggc agaatcaatc
     1981 aattgatgtt gaaccttcaa tgtaggaaat tcgttcttga tggtttcctc cacagttttt
     2041 ctccataatc ttgaagaggc caaaacatta gctttatcca aggaccaaat aggcaatggt
     2101 ggctcatgtt gtagggccat gaaagcggcc attcttgtga ttctttgcac ttctggaacg
     2161 gtgtattgtt cactatccca agcgacacca tcaccatcgt cttcctttct cttaccaaag
     2221 taaatacctc ccactaattc tctgacaaca acgaagtcag tacctttagc aaattgtggc
     2281 ttgattggag ataagtctaa aagagagtcg gatgcaaagt tacatggtct taagttggcg
     2341 tacaattgaa gttctttacg gatttttagt aaaccttgtt caggtctaac actacctgta
     2401 ccccatttag gaccacccac agcacctaac aaaacggcat caaccttctt ggaggcttcc
     2461 agcgcctcat ctggaagtgg gacacctgta gcttcgaaag cagcaccacc aattaaatga
     2521 ttttcgaaat cgaacttgac attggaacga acatcagaaa tagctttaag aaccttaatg
     2581 gcttcggctg tgatttcttg accaacgtgg tcacctggca aaacgacgat cttcttaggg
     2641 gcagacatta gaatggtata tccttgaaat atatatatat attgctgaaa tgtaaaaggt
     2701 aagaaaagtt agaaagtaag acgattgcta accacctatt ggaaaaaaca ataggtcctt
     2761 aaataatatt gtcaacttca agtattgtga tgcaagcatt tagtcatgaa cgcttctcta
     2821 ttctatatga aaagccggtt ccggcctctc acctttcctt tttctcccaa tttttcagtt
     2881 gaaaaaggta tatgcgtcag gcgacctctg aaattaacaa aaaatttcca gtcatcgaat
     2941 ttgattctgt gcgatagcgc ccctgtgtgt tctcgttatg ttgaggaaaa aaataatggt
     3001 tgctaagaga ttcgaactct tgcatcttac gatacctgag tattcccaca gttggggatc
     3061 tcgactctag ctagaggatc aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     3121 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3181 gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3241 cgggaaacct gtcgtgccag ctggattaat gaatcggcca acgcgcgggg agaggcggtt
     3301 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3361 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3421 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3481 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3541 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3601 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3661 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3721 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3781 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3841 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3901 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     3961 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4021 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4081 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4141 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4201 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4261 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4321 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4381 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4441 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4501 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4561 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4621 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4681 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4741 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4801 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4861 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4921 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4981 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5041 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     5101 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     5161 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5221 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     5281 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     5341 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     5401 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     5461 actatgcggc atcagagcag attgtactga gagtgcacca taacgcattt aagcataaac
     5521 acgcactatg ccgttcttct catgtatata tatatacagg caacacgcag atataggtgc
     5581 gacgtgaaca gtgagctgta tgtgcgcagc tcgcgttgca ttttcggaag cgctcgtttt
     5641 cggaaacgct ttgaagttcc tattccgaag ttcctattct ctagctagaa agtataggaa
     5701 cttcagagcg cttttgaaaa ccaaaagcgc tctgaagacg cactttcaaa aaaccaaaaa
     5761 cgcaccggac tgtaacgagc tactaaaata ttgcgaatac cgcttccaca aacattgctc
     5821 aaaagtatct ctttgctata tatctctgtg ctatatccct atataaccta cccatccacc
     5881 tttcgctcct tgaacttgca tctaaactcg acctctacat tttttatgtt tatctctagt
     5941 attactcttt agacaaaaaa attgtagtaa gaactattca tagagtgaat cgaaaacaat
     6001 acgaaaatgt aaacatttcc tatacgtagt atatagagac aaaatagaag aaaccgttca
     6061 taattttctg accaatgaag aatcatcaac gctatcactt tctgttcaca aagtatgcgc
     6121 aatccacatc ggtatagaat ataatcgggg atgcctttat cttgaaaaaa tgcacccgca
     6181 gcttcgctag taatcagtaa acgcgggaag tggagtcagg ctttttttat ggaagagaaa
     6241 atagacacca aagtagcctt cttctaacct taacggacct acagtgcaaa aagttatcaa
     6301 gagactgcat tatagagcgc acaaaggaga aaaaaagtaa tctaagatgc tttgttagaa
     6361 aaatagcgct ctcgggatgc atttttgtag aacaaaaaag aagtatagat tctttgttgg
     6421 taaaatagcg ctctcgcgtt gcatttctgt tctgtaaaaa tgcagctcag attctttgtt
     6481 tgaaaaatta gcgctctcgc gttgcatttt tgttttacaa aaatgaagca cagattcttc
     6541 gttggtaaaa tagcgctttc gcgttgcatt tctgttctgt aaaaatgcag ctcagattct
     6601 ttgtttgaaa aattagcgct ctcgcgttgc atttttgttc tacaaaatga agcacagatg
     6661 cttcgtt
//
