LOCUS       V006129                 5334 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006129
VERSION     V006129
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Li Y, Rosso MG, Strizhov N, Viehoever P, Weisshaar B.
  TITLE     GABI-Kat SimpleSearch: a flanking sequence tag (FST) database for
            the identification of T-DNA insertion mutants in Arabidopsis
            thaliana
  JOURNAL   Bioinformatics 19 (11), 1441-1442 (2003)
   PUBMED   12874060
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Rosso MG, Li Y, Strizhov N, Reiss B, Dekker K, Weisshaar B.
  TITLE     An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for
            flanking sequence tag-based reverse genetics
  JOURNAL   Plant Mol. Biol. 53 (1-2), 247-259 (2003)
   PUBMED   14756321
REFERENCE   3  (bases 1 to 5334)
  AUTHORS   Rosso MG, Li Y, Strizhov N, Weisshaar B.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2004) Dept. Salamini, Max-Planck-Institute for
            Plant Breeding Research, Carl-von-Linne-Weg 10, Koeln D-50829,
            Germany
REFERENCE   4  (bases 1 to 5334)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5334)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Bioinformatics"; date: "2003"; volume: "19"; issue: "11"; pages:
            "1441-1442"
            SGRef: number: 2; type: "Journal Article"; journalName: "Plant Mol.
            Biol. 53 (1-2), 247-259 (2003)"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (19-JAN-2004) Dept. Salamini, Max-Planck-Institute for Plant
            Breeding Research, Carl-von-Linne-Weg 10, Koeln D-50829, Germany"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      31..740
                     /label="oriV"
                     /note="incP origin of replication"
     promoter        813..904
                     /label="AmpR promoter"
     CDS             905..1762
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1936..2524
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    2832..2856
                     /label="LB T-DNA repeat"
                     /note="left border repeat from octopine Ach5 T-DNA"
     promoter        complement(3546..3926)
                     /label="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     regulatory      4073..4243
                     /label="pea rubisco targeting signal"
                     /note="pea rubisco targeting signal"
                     /regulatory_class="other"
     CDS             4244..5080
                     /gene="sulI"
                     /label="Dihydropteroate synthase type-1"
                     /note="Dihydropteroate synthase type-1 from Escherichia
                     coli. Accession#: P0C002"
     misc_feature    5311..5334
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ctcgagcacc gacgagcaag gcaagaccga gcgcctttcc gacgctcacc gggctggttg
       61 ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga
      121 agccgtgtgc gagacaccgg ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc
      181 actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg
      241 acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc
      301 ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata
      361 agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc
      421 ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg
      481 ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc
      541 taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc
      601 tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc
      661 ccggcccgct aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg
      721 cctcacccca aaaatggcag cgctggcagt ccttgccatt gccgggatcg gggcagtaac
      781 gggatgggcg atcagcccga aagctacccc tatttgttta tttttctaaa tacattcaaa
      841 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      901 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      961 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     1021 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     1081 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     1141 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     1201 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1261 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1321 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1381 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     1441 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     1501 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     1561 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     1621 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     1681 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     1741 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1801 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1861 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1921 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1981 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2041 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2101 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2161 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2221 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2281 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2341 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2401 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2461 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2521 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2581 catggactct agaggatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
     2641 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
     2701 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
     2761 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
     2821 tggctctcga gcggcaggat atattcaatt gtaaatggct tcatgtccgg gaaatctaca
     2881 tggatcagca atgagtatga tggtcaatat ggagaaaaag aaagagtaat taccaatttt
     2941 ttttcaattc aaaaatgtag atgtccgcag cgttattata aaatgaaagt acattttgat
     3001 aaaacgacaa attacgatcc gtcgtattta taggcgaaag caataaacaa attattctaa
     3061 ttcggaaatc tttatttcga cgtgtctaca ttcacgtcca aatgggggct tagatgagaa
     3121 acttcacgat cgatgccttg atttcgccat tcccagatac ccatttcatc ttcagattgg
     3181 tctgagatta tgcgaaaata tacactcata tacataaata ctgacagttt gagctaccaa
     3241 ttcagtgtag cccattacct tacataattc actcaaatgc taggcagtct gtcaactcgg
     3301 cgtcaatttg tcggccacta tacgatagtt gcgcaaattt tcaaagtcct ggcctaacat
     3361 cacacctctg tcggcggcgg gtcccatttg tgataaatcc accatcacaa tagatagtct
     3421 aatggacgaa aaaggcgaat atttcgatgc tgagattcga cgcaattaat tcgagaaaaa
     3481 tcccgtgatt gatgctgttg agttaccaat aatatgggca gcgaaggcca tttaattata
     3541 agatccgatt tggtgtatcg agattggtta tgaaattcag atgctagtgt aatgtattgg
     3601 taatttggga agatataata ggaagcaagg ctatttatcc atttctgaaa aggcgaaatg
     3661 gcgtcaccgc gagcgtcacg cgcattccgt tcttgctgta aagcgttgtt tggtacactt
     3721 ttgactagcg aggcttggcg tgtcagcgta tctattcaaa agtcgttaat ggctgcggat
     3781 caagaaaaag ttggaataga aacagaatac ccgcgaaatt caggcccggt tgccatgtcc
     3841 tacacgccga aataaacgac caaattagta gaaaaataaa aactgactcg gatacttacg
     3901 tcacgtcttg cgcactgatt tgaaaaatct caatataaac aaagacggcc acaagaaaaa
     3961 accaaaacac cgatattcat taatcttatc tagtttctca aaaaaattca tatcttccac
     4021 acgtggatcg atccgtcgta ccagctttgc aattcataca gaagtgagaa aaatggcttc
     4081 tatgatatcc tcttcagctg tgactacagt cagccgtgct tctacggtgc aatcggccgc
     4141 ggtggctcca ttcggcggcc tcaaatccat gactggattc ccagttaaga aggtcaacac
     4201 tgacattact tccattacaa gcaatggtgg aagagtaaag tgcatggtga cggtgttcgg
     4261 cattctgaat ctcaccgagg actccttctt cgatgagagc cggcggctag accccgccgg
     4321 cgctgtcacc gcggcgatcg aaatgctgcg agtcggatca gacgtcgtgg atgtcggacc
     4381 ggccgccagc catccggacg cgaggcctgt atcgccggcc gatgagatca gacgtattgc
     4441 gccgctctta gacgccctgt ccgatcagat gcaccgtgtt tcaatcgaca gcttccaacc
     4501 ggaaacccag cgctatgcgc tcaagcgcgg cgtgggctac ctgaacgata tccaaggatt
     4561 tcctgaccct gcgctctatc ccgatattgc tgaggcggac tgcaggctgg tggttatgca
     4621 ctcagcgcag cgggatggca tcgccacccg caccggtcac cttcgacccg aagacgcgct
     4681 cgacgagatt gtgcggttct tcgaggcgcg ggtttccgcc ttgcgacgga gcggggtcgc
     4741 tgccgaccgg ctcatcctcg atccggggat gggatttttc ttgagccccg caccggaaac
     4801 atcgctgcac gtgctgtcga accttcaaaa gctgaagtcg gcgttggggc ttccgctatt
     4861 ggtctcggtg tcgcggaaat ccttcttggg cgccaccgtt ggccttcctg taaaggatct
     4921 gggtccagcg agccttgcgg cggaacttca cgcgatcggc aatggcgctg actacgtccg
     4981 cacccacgcg cctggagatc tgcgaagcgc aatcaccatc tcggaaaccc tcgcgaaatt
     5041 tcgcagtcgc gacgccagag accgagggtt agatcatgcc tagcttggct gcaggtcgac
     5101 ctgtacaggc atgcccgctg aaatcaccag tctctctcta caaatctatc tctctctata
     5161 ataatgtgtg agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt
     5221 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
     5281 aatttctaat tcctaaaacc aaaatccagg tgacaggata tattggcggg taaa
//
