LOCUS       40924_20870       11601 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pGA643, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   An G, Ebert PR, Mitra A, Ha SB.
  TITLE     Binary Vectors
  JOURNAL   (in) Gelvin,S.B. and Schilperoort,R.A. (Eds.); PLANT MOLECULAR 
            BIOLOGY MANUAL: A3/1-19; Kluwer Academic Publishers (1988)
REFERENCE   2  (bases 1 to 11601)
  AUTHORS   Kim S-R., Kang H-G., Lee S, Kim K-M., An G.
  TITLE     Complete sequence of the pGA643 binary vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 11601)
  AUTHORS   Kim S-R., Kang H-G., Lee S, Kim K-M., An G.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2004) Division of Molecular and Life Science, 
            POSTECH, Nam-Gu Hyoja San31, Pohang, Kyungbuk 790-784, Republic of 
            Korea
REFERENCE   4  (bases 1 to 11601)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 11601)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "(in) 
            Gelvin,S.B. and Schilperoort,R.A. (Eds.)"; volume: " PLANT MOLECULAR
            BIOLOGY MANUAL"; pages: " A3/1-19; Kluwer Academic Publishers (1988"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (01-NOV-2004) Division of Molecular and Life Science, POSTECH, 
            Nam-Gu Hyoja San31, Pohang, Kyungbuk 790-784, Republic of Korea"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..25)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        181..364
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             365..1183
                     /codon_start=1
                     /label=KanR
                     /note="fusion between an N-terminal peptide of nopaline
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      1811..2063
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2471..2815
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    2824..2862
                     /label=polylinker
                     /note="polylinker"
     terminator      2875..3075
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti
                     plasmid gene 7"
     misc_feature    complement(3076..3601)
                     /label=3'UTR of T-DNA gene 5
                     /note="3'UTR of T-DNA gene 5"
     misc_feature    complement(3678..3702)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     oriT            4257..4366
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             4399..4767
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     CDS             5042..6187
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(7466..8662)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             8768..9415
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     rep_origin      complement(10413..11122)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
      121 acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagccgc
      181 gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa
      241 agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt
      301 accataaatt cccctcggta tccaattaga gtctcatatt cactctcaat ccaaataatc
      361 tgcaatggca attaccttat ccgcaacttc tttacctatt tccgcccgga tccgggcagg
      421 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
      481 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
      541 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
      601 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
      661 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
      721 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
      781 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
      841 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
      901 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
      961 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     1021 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     1081 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     1141 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     1201 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     1261 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     1321 cagcgcgggg atctcatgct ggagttcttc gcccaccccg atccaacact tacgtttgca
     1381 acgtccaaga gcaaatagac cacgaacgcc ggaaggttgc cgcagcgtgt ggattgcgtc
     1441 tcaattctct cttgcaggaa tgcaatgatg aatatgatac tgactatgaa actttgaggg
     1501 aatactgcct agcaccgtca cctcataacg tgcatcatgc atgccctgac aacatggaac
     1561 atcgctattt ttctgaagaa ttatgctcgt tggaggatgt cgcggcaatt gcagctattg
     1621 ccaaaatcga aatacccctc acgcatgcat tcatcaatat tattcatgcg gggaaaggca
     1681 agattaatcc aactggcaaa tcatccagcg tgattggtaa cttcagttcc agcgacttga
     1741 ttcgttttgg tgctacccac gttttcaata aggacgagat ggtggagtaa agaaggagtg
     1801 cgtcgaagca gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     1861 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     1921 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     1981 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     2041 ggtgtcatct atgttactag atcgatcaaa cttcggtact gtgtaatgac gatgagcaat
     2101 cgagaggctg actaacaaaa ggtatgccca aaaacaacct ctccaaactg tttcgaattg
     2161 gaagtttctg ctcatgccga caggcataac ttagatattc gcgggctatt cccactaatt
     2221 cgtcctgctg gtttgcgcca agataaatca gtgcatctcc ttacaagttc ctctgtcttg
     2281 tgaaatgaac tgctgactgc cccccaagaa agcctcctca tctcccagtt ggcggcggct
     2341 gatacaccat cgaaaaccca cgtccgaaca cttgatacat gtgcctgaga aataggaaca
     2401 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
     2461 aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
     2521 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat
     2581 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca
     2641 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     2701 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     2761 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggaccctcg
     2821 accaagcttc tagagctcgt taacggtacc atcgatagat ctgcgatgag ctaagctagc
     2881 tatatcatca atttatgtat tacacataat atcgcactca gtctttcatc tacggcaatg
     2941 taccagctga tataatcagt tattgaaata tttctgaatt taaacttgca tcaataaatt
     3001 tatgtttttg cttggactat aatacctgac ttgttatttt atcaataaat atttaaacta
     3061 tatttctttc aagatatcat tctttacaag tatacgtgtt taaattgaat accataaatt
     3121 tttatttttc aaatacatgt aaaattatga aatgggagtg gtggcgaccg agctcaagca
     3181 cacttcaatt cctataacgg accaaatcgc aaaaattata ataacatatt atttcatcct
     3241 ggattaaaag aaagtcaccg gggattattt tgtgacgccg attacatacg gcgacaataa
     3301 agacattgga aatcgtagta catattggaa tacactgatt atattaatga tgaatacata
     3361 ctttaatatc cttacgtagg atcaacatat cttgttacaa tcggacactt ttgcttcatc
     3421 cccgctaaca cctctgcacc ttagaccaag cgcttccaca aggaactgag agccatagcc
     3481 cacctcacct tgggttcctt tggccgcctg tctttctgaa agagagcctt gcccaccgca
     3541 actatttcaa cacagatagg atcaacccgg gatggcgcta agaagctatt gccgccgatc
     3601 ttcatagggt accgagctcg aattcagtac attaaaaacg tccgcaatgt gttattaagt
     3661 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
     3721 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
     3781 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
     3841 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
     3901 gttgattgta acgatgacag agcgttgctg cctgtgatca aatatcatct ccctcgcaga
     3961 gatccgaatt atcagccttc ttattcattt ctcgcttaac cgtgacaggc tgtcgatctt
     4021 gagaactatg ccgacataat aggaaatcgc tggataaagc cgctgaggaa gctgagtggc
     4081 gctatttctt tagaagtgaa cgttgacgat cgtcgacgga tcttttccgc tgcataaccc
     4141 tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc acgatataca
     4201 ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg tcagccgggc
     4261 aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg
     4321 cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccggctac cgccggcgta
     4381 acagatgagg gcaagcggat ggctgatgaa accaagccaa ccaggaaggg cagcccacct
     4441 atcaaggtgt actgccttcc agacgaacga agagcgattg aggaaaaggc ggcggcggcc
     4501 ggcatgagcc tgtcggccta cctgctggcc gtcggccagg gctacaaaat cacgggcgtc
     4561 gtggactatg agcacgtccg cgagctggcc cgcatcaatg gcgacctggg ccgcctgggc
     4621 ggcctgctga aactctggct caccgacgac ccgcgcacgg cgcggttcgg tgatgccacg
     4681 atcctcgccc tgctggcgaa gatcgaagag aagcaggacg agcttggcaa ggtcatgatg
     4741 ggcgtggtcc gcccgagggc agagccatga cttttttagc cgctaaaacg gccggggggt
     4801 gcgcgtgatt gccaagcacg tccccatgcg ctccatcaag aagagcgact tcgcggagct
     4861 ggtattcgtg cagggcaaga ttcggaatac caagtacgag aaggacggcc agacggtcta
     4921 cgggaccgac ttcattgccg ataaggtgga ttatctggac accaaggcac caggcgggtc
     4981 aaatcaggaa taagggcaca ttgccccggc gtgagtcggg gcaatcccgc aaggagggtg
     5041 aatgaatcgg acgtttgacc ggaaggcata caggcaagaa ctgatcgacg cggggttttc
     5101 cgccgaggat gccgaaacca tcgcaagccg caccgtcatg cgtgcgcccc gcgaaacctt
     5161 ccagtccgtc ggctcgatgg tccagcaagc tacggccaag atcgagcgcg acagcgtgca
     5221 actggctccc cctgccctgc ccgcgccatc ggccgccgtg gagcgttcgc gtcgtctcga
     5281 acaggaggcg gcaggtttgg cgaagtcgat gaccatcgac acgcgaggaa ctatgacgac
     5341 caagaagcga aaaaccgccg gcgaggacct ggcaaaacag gtcagcgagg ccaagcaggc
     5401 cgcgttgctg aaacacacga agcagcagat caaggaaatg cagctttcct tgttcgatat
     5461 tgcgccgtgg ccggacacga tgcgagcgat gccaaacgac acggcccgct ctgccctgtt
     5521 caccacgcgc aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca ttttccacgt
     5581 caacaaggac gtgaagatca cctacaccgg cgtcgagctg cgggccgacg atgacgaact
     5641 ggtgtggcag caggtgttgg agtacgcgaa gcgcacccct atcggcgagc cgatcacctt
     5701 cacgttctac gagctttgcc aggacctggg ctggtcgatc aatggccggt attacacgaa
     5761 ggccgaggaa tgcctgtcgc gcctacaggc gacggcgatg ggcttcacgt ccgaccgcgt
     5821 tgggcacctg gaatcggtgt cgctgctgca ccgcttccgc gtcctggacc gtggcaagaa
     5881 aacgtcccgt tgccaggtcc tgatcgacga ggaaatcgtc gtgctgtttg ctggcgacca
     5941 ctacacgaaa ttcatatggg agaagtaccg caagctgtcg ccgacggccc gacggatgtt
     6001 cgactatttc agctcgcacc gggagccgta cccgctcaag ctggaaacct tccgcctcat
     6061 gtgcggatcg gattccaccc gcgtgaagaa gtggcgcgag caggtcggcg aagcctgcga
     6121 agagttgcga ggcagcggcc tggtggaaca cgcctgggtc aatgatgacc tggtgcattg
     6181 caaacgctag ggccttgtgg ggtcagttcc ggctgggggt tcagcagcca gcgctttact
     6241 ggcatttcag gaacaagcgg gcactgctcg acgcacttgc ttcgctcagt atcgctcggg
     6301 acgcacggcg cgctctacga actgccgata aacagaggat taaaattgac aattgtgatt
     6361 aaggctcaga ttcgacggct tggagcggcc gacgtgcagg atttccgcga gatccgattg
     6421 tcggccctga agaaagctcc agagatgttc gggtccgttt acgagcacga ggagaaaaag
     6481 cccatggagg cgttcgctga acggttgcga gatgccgtgg cattcggcgc ctacatcgac
     6541 ggcgagatca ttgggctgtc ggtcttcaaa caggaggacg gccccaagga cgctcacaag
     6601 gcgcatctgt ccggcgtttt cgtggagccc gaacagcgag gccgaggggt cgccggtatg
     6661 ctgctgcggg cgttgccggc gggtttattg ctcgtgatga tcgtccgaca gattccaacg
     6721 ggaatctggt ggatgcgcat cttcatcctc ggcgcactta atatttcgct attctggagc
     6781 ttgttgttta tttcggtcta ccgcctgccg ggcggggtcg cggcgacggt aggcgctgtg
     6841 cagccgctga tggtcgtgtt catctctgcc gctctgctag gtagcccgat acgattgatg
     6901 gcggtcctgg gggctatttg cggaactgcg ggcgtggcgc tgttggtgtt gacaccaaac
     6961 gcagcgctag atcctgtcgg cgtcgcagcg ggcctggcgg gggcggtttc catggcgttc
     7021 ggaaccgtgc tgacccgcaa gtggcaacct cccgtgcctc tgctcacctt taccgcctgg
     7081 caactggcgg ccggaggact tctgctcgtt ccagtagctt tagtgtttga tccgccaatc
     7141 ccgatgccta caggaaccaa tgttctcggc ctggcgtggc tcggcctgat cggagcgggt
     7201 ttaacctact tcctttggtt ccgggggatc tcgcgactcg aacctacagt tgtttcctta
     7261 ctgggctttc tcagcccggg gaccgccgtg ttgctaggat ggttgttctt ggatcagacg
     7321 ctgagtgcgc ttcaaatcat cggcgtcctg ctcgtgatcg ggagtatctg gctgggccaa
     7381 cgttccaacc gcactcctag ggcgcgtata gcttgccgga agtcgccttg acccgcatgg
     7441 cataggccta tcgtttccac gatcagcgat cggctcgttg ccctgcgccg ctccaaagcc
     7501 cgcgacgcag cgccggcagg cagagcaagt agagggcagc gcctgcaatc catgcccacc
     7561 cgttccacgt tgttatagaa gccgcataga tcgccgtgaa gaggaggggt ccgacgatcg
     7621 aggtcaggct ggtgagcgcc gccagtgagc cttgcagctg cccctgacgt tcctcatcca
     7681 cctgcctgga caacattgct tgcagcgccg gcattccgat gccacccgaa gcaagcagga
     7741 ccatgatcgg gaacgccatc catccccgtg tcgcgaaggc aagcaggatg tagcctgtgc
     7801 cgtcggcaat cattccgagc atgagtgccc gcctttcgcc gagccgggcg gctacagggc
     7861 cggtgatcat tgcctgggcg agtgaatgca gaatgccaaa tgcggcaagc gaaatgccga
     7921 tcgtggtcgc gtcccagtga aagcgatcct cgccgaaaat gacccaaagc gcggccggca
     7981 cctgtccgac aagttgcatg atgaagaaga ccgccatcag ggcggcgacg acggtcatgc
     8041 cccgggccca ccggaacgaa gcgagcgggt tgagagcctc ccggcgtaac ggccggcgtt
     8101 cgcctttgtg cgactccggc aaaaggaaac agcccgtcag gaaattgagg ccgttcaagg
     8161 ctgccgcggc gaagaacgga gcgtgggggg agaaaccgcc catcagccca ccgagcacag
     8221 gtcccgcgac catcccgaac ccgaaacagg cgctcatgaa gccgaagtgc cgcgcgcgct
     8281 catcgccatc agtgatatcg gcaatataag cgccggctac cgccccagtc gccccggtga
     8341 tgccggccac gatccgcccg atatagagaa cccaaaggaa aggcgctgtc gccatgatgg
     8401 cgtagtcgac agtggcgccg gccagcgaga cgagcaagat tggccgccgc ccgaaacgat
     8461 ccgacagcgc gcccagcaca ggtgcgcagg caaattgcac caacgcatac agcgccagca
     8521 gaatgccata gtgggcggtg acgtcgttcg agtgaaccag atcgcgcagg aggcccggca
     8581 gcaccggcat aatcaggccg atgccgacag cgtcgagcgc gacagtgctc agaattacga
     8641 tcaggggtat gttgggtttc atgtctggcc tccggaccag cctccgctgg tccgattgaa
     8701 cgcgcggatt ctttatcact gataagttgg tggacatatt atgtttatca gtgataaagt
     8761 gtcaagcatg acaaagttgc agccgaatac agtgatccgt gccgccctgg acctgttgaa
     8821 cgaggtcggc gtagacggtc tgacgacacg caaactggcg gaacggttgg gggttcagca
     8881 gccggcgctt tactggcact tcaggaacaa gcgggcgctg ctcgacgcac tggccgaagc
     8941 catgctggcg gagaatcata cgcattcggt gccgagagcc gacgacgact ggcgctcatt
     9001 tctgatcggg aatgcccgca gcttcaggca ggcgctgctc gcctaccgcg atggcgcgcg
     9061 catccatgcc ggcacgcgac cgggcgcacc gcagatggaa acggccgacg cgcagcttcg
     9121 cttcctctgc gaggcgggtt tttcggccgg ggacgccgtc aatgcgctga tgacaatcag
     9181 ctacttcact gttggggccg tgcttgagga gcaggccggc gacagcgatg ccggcgagcg
     9241 cggcggcacc gttgaacagg ctccgctctc gccgctgttg cgggccgcga tagacgcctt
     9301 cgacgaagcc ggtccggacg cagcgttcga gcagggactc gcggtgattg tcgatggatt
     9361 ggcgaaaagg aggctcgttg tcaggaacgt tgaaggaccg agaaagggtg acgattgatc
     9421 aggaccgctg ccggagcgca acccactcac tacagcagag ccatgtagac aacatcccct
     9481 ccccctttcc accgcgtcag acgcccgtag cagcccgcta cgggcttttt catgccctgc
     9541 cctagcgtcc aagcctcacg gccgcgctcg gcctctctgg cggccttctg gcgctcctgc
     9601 tgcggcgtcc gctcgtgggc cgtggcgcgg gtccgcgcgc cggcctcgtg cgcctggcgc
     9661 tcgcgggcga ggtccagggc ggccgtcttc acgttctgcc ttgcgcagat gagatagatc
     9721 cagtctgcaa gtgtcagaaa cgagtctgca tttgatgccc tgaacgtctt catacatctg
     9781 ataagcccct cccggaagtc tgctttctct ccgtcagaaa gcataccctg accggtttca
     9841 atcacggacc gttctgtcag gtaatgctct gtccagtcat acagagccgg gtttgtcacc
     9901 cgtataagct gaagcaggca caaatcaggg aaataaacaa aatcccgcat ccccggataa
     9961 agaaaaatca gggaattaat ggcctgatgg atttcccgtg gcgtcgaaag tgcggcacca
    10021 taggtatcag tcaccgcgat gagatccctt accattccag agtctggcgg ttgattatta
    10081 atttgctgat atagagcctc agcccgctgg cgaaattcat tacgtaaatc aaaggcttca
    10141 ggtcggggta atttaaaact aagctgaatg attttctgga gataacggct gccatcttcg
    10201 atattcagcg catgttcaac ggcatgagtg ataatctgcc tgtcataaca gagaatatgg
    10261 gtaaagcggg gcagatcggc tactgcacgc acaagcctga acacttccgc cacctgggat
    10321 ggctccagtc ggtccagatc atccatgaca acaatgaact tcagatccag actcaccagt
    10381 tgtcctgcaa tttcagcccg aagctagctt ggctgccatt tttggggtga ggccgttcgc
    10441 ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc gggagggttc
    10501 gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt
    10561 taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc
    10621 cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa
    10681 atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg
    10741 cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca
    10801 ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg
    10861 aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg
    10921 cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca
    10981 agttttccgc gaggtatcca caacgccggc ggccgcggtg tctcgcacac ggcttcgacg
    11041 gcgtttctgg cgcgtttgca gggccataga cggccgccag cccagcggcg agggcaacca
    11101 gcccggtgag cgtcggaaag ggtcgacgat cttgctgcgt tcggatattt tcgtggagtt
    11161 cccgccacag acccggattg aaggcgagat ccagcaactc gcgccagatc atcctgtgac
    11221 ggaactttgg cgcgtgatga ctggccagga cgtcggccga aagagcgaca agcagatcac
    11281 gcttttcgac agcgtcggat ttgcgatcga ggatttttcg gcgctgcgct acgtccgcga
    11341 ccgcgttgag ggatcaagcc acagcagccc actcgacctt ctagccgacc cagacgagcc
    11401 aagggatctt tttggaatgc tgctccgtcg tcaggctttc cgacgtttgg gtggttgaac
    11461 agaagtcatt atcgcacgga atgccaagca ctcccgaggg gaaccctgtg gttggcatgc
    11521 acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta
    11581 ataaacgctc ttttctctta g
//
