LOCUS       40924_20865        3893 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pGA3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ross TM, Xu Y, Bright RA, Robinson HL.
  TITLE     C3d enhancement of antibodies to hemagglutinin accelerates 
            protection against influenza virus challenge
  JOURNAL   Nat. Immunol. 1 (2), 127-131 (2000)
  PUBMED    11248804
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Hua J, Bright R, Ross T.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2001) Microbiology and Immunology, Yerkes Regional
            Primate Research Center, 954 Gatewood RD NE, Atlanta, GA 30329, USA
REFERENCE   3  (bases 1 to 3893)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3893)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Immunol."; date: "2000"; volume: "1"; issue: "2"; pages: "127-131"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-SEP-2001) Microbiology and Immunology, Yerkes Regional Primate 
            Research Center, 954 Gatewood RD NE, Atlanta, GA 30329, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        93..472
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        473..676
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          812..1637
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     misc_feature    1668..1721
                     /label=tpa leader
                     /note="tpa leader"
     polyA_signal    1760..1982
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     terminator      complement(1983..2017)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2038..2829)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      3241..3829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgacaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
       61 tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata
      121 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      181 tacggtaaat ggcccgcctg gctgaccccc caacgacccc cgcccattga cgtcaataat
      241 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      301 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
      361 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
      421 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      481 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      541 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      601 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
      661 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
      721 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat
      781 tggaacgcgg attccccgtg ccaagagtga cgtaagtacc gcctatagac tctataggca
      841 cacccctttg gctcttatgc atgctatact gtttttggct tggggcctat acacccccgc
      901 ttccttatgc tataggtgat ggtatagctt agcctatagg tgtgggttat tgaccattat
      961 tgaccactcc cctattggtg acgatacttt ccattactaa tccataacat ggctctttgc
     1021 cacaactatc tctattggct atatgccaat actctgtcct tcagagactg acacggactc
     1081 tgtattttta caggatgggg tcccatttat tatttacaaa ttcacatata caacaacgcc
     1141 gtcccccgtg cccgcagttt ttattaaaca tagcgtggga tctccacgcg aatctcgggt
     1201 acgtgttccg gacatgggct cttctccggt agcggcggag cttccacatc cgagccctgg
     1261 tcccatgcct ccagcggctc atggtcgctc ggcagctcct tgctcctaac agtggaggcc
     1321 agacttaggc acagcacaat gcccaccacc accagtgtgc cgcacaaggc cgtggcggta
     1381 gggtatgtgt ctgaaaatga gctcggagat tgggctcgca ccgctgacgc agatggaaga
     1441 cttaaggcag cggcagaaga agatgcaggc agctgagttg ttgtattctg ataagagtca
     1501 gaggtaactc ccgttgcggt gctgttaacg gtggagggca gtgtagtctg agcagtactc
     1561 gttgctgccg cgcgcgccac cagacataat agctgacaga ctaacagact gttcctttcc
     1621 atgggtcttt tctgcagtca ccgtccaagc ttgcaatcat ggatgcaatg aagagagggc
     1681 tctgctgtgt gctgctgctg tgtggagcag tcttcgtttc ggctagcccc gggtgataag
     1741 gatcctcgca atccctaggc tgtgccttct agttgccagc catctgttgt ttgcccctcc
     1801 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     1861 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     1921 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     1981 atataaaaaa cgcccggcgg caaccgagcg ttctgaacgc tagagtcgac aaattcagaa
     2041 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
     2101 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
     2161 caacgctatg tcctgatagc ggtctgccac acccagccgg ccacagtcga tgaatccaga
     2221 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
     2281 atcctcgccg tcgggcatgc tcgccttgag cctggcgaac agttcggctg gcgcgagccc
     2341 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
     2401 tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg
     2461 cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga
     2521 caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac
     2581 aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc
     2641 ctcgtcttgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg
     2701 cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca
     2761 gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg
     2821 ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca
     2881 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
     2941 agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag
     3001 ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct
     3061 tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc
     3121 tttctacgtg aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3181 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3241 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3301 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3361 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3421 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3481 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3541 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3601 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3661 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3721 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3781 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3841 cgcggccctt ttacggttcc tggccttttg ctggcctttt gctcacatgt tgt
//
