LOCUS       40924_20855        3898 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pGA1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ross TM, Xu Y, Bright RA, Robinson HL.
  TITLE     C3d enhancement of antibodies to hemagglutinin accelerates 
            protection against influenza virus challenge
  JOURNAL   Nat. Immunol. 1 (2), 127-131 (2000)
  PUBMED    11248804
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Robinson HL, Hua J, Ross TM, Smith JM.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2001) Microbiology and Immunology, Yerkes Regional
            Primate Research Center, 954 Gatewood RD NE, Atlanta, GA 30329, USA
REFERENCE   3  (bases 1 to 3898)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3898)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Immunol."; date: "2000"; volume: "1"; issue: "2"; pages: "127-131"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-SEP-2001) Microbiology and Immunology, Yerkes Regional Primate 
            Research Center, 954 Gatewood RD NE, Atlanta, GA 30329, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        93..474
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        475..678
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          814..1640
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     misc_feature    1659..1721
                     /label=tpa leader
                     /note="tpa leader"
     polyA_signal    1764..1986
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     terminator      complement(1987..2021)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2042..2833)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      3245..3834
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgacaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
       61 tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata
      121 gtaatcaatt acgggttcat tagttcatag cccatatatg gagttccgcg ttacataact
      181 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      241 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      301 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
      361 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
      421 ggactttcct acttggcagt acatctacgg tattagtcat cggctattac catggtgatg
      481 cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      541 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      601 aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag
      661 gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
      721 tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc
      781 attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag actctatagg
      841 cacacccctt tggctcttat gcatgctata ctgtttttgg cttggggcct atacaccccc
      901 gcttccttat gctataggtg atggtatagc ttagcctata ggtgtgggtt attgaccatt
      961 attgaccact cccctattgg tgacgatact ttccattact aatccataac atggctcttt
     1021 gccacaacta tctctattgg ctatatgcca atactctgtc cttcagagac tgacacggac
     1081 tctgtatttt tacaggatgg ggtcccattt attatttaca aattcacata tacaacaacg
     1141 ccgtcccccg tgcccgcagt ttttattaaa catagcgtgg gatctccacg cgaatctcgg
     1201 gtaccgtgtt ccggacatgg gytcttctcc ggtagcggcg gagcttccac atccgagccc
     1261 tggtcccatg cctccagcgg ctcatggtcg ctcggcagct ccttgctcct aacagtggag
     1321 gccagactta ggcacagcac aatgcccacc accaccagtg tgccgcacaa ggccgtggcg
     1381 gtagggtatg tgtctgaaaa tgagctcgga gattgggctc gcaccgctga cgcagatgga
     1441 agacttaagg cagcggcaga agaagatgca ggcagctgag ttgttgtatt ctgataagag
     1501 tcagaggtaa ctcccgttgc ggtgctgtta acggtggagg gcagtgtagt ctgagcagta
     1561 ctcgttgctg ccgcgcgcgc caccagacat aatagctgac agactaacag actgttcctt
     1621 tccatgggtc ttttctgcag tcaccatcga tgcttgcaat catggatgca atgaagagag
     1681 ggctctgctg tgtgctgctg ctgtgtggag cagtcttcgt ttcggctagc cccgggtgat
     1741 aaacggaccg cgcaatccct aggctgtgcc ttctagttgc cagccatctg ttgtttgccc
     1801 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     1861 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     1921 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     1981 ctctatataa aaaacgcccg gcggcaaccg agcgttctga acgctagagt cgacaaattc
     2041 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     2101 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     2161 tagccaacgc tatgtcctga tagcggtctg ccacacccag ccggccacag tcgatgaatc
     2221 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
     2281 cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg gctggcgcga
     2341 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
     2401 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
     2461 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
     2521 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
     2581 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
     2641 ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
     2701 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
     2761 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
     2821 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc
     2881 gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct
     2941 taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt
     3001 ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt
     3061 cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac
     3121 tggctttcta cgtgaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     3181 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3241 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3301 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3361 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3421 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3481 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3541 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3601 cgacctacac ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3661 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3721 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3781 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3841 agcaacgcgg cccttttacg gttcctggcc ttttgctggc cttttgctca catgttgt
//
