LOCUS       40924_20850        4955 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             225..1874
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(1918..2039)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2458..3046)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3220..4077)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4078..4182)
                     /label=AmpR promoter
     rep_origin      4209..4664
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4795..4843
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4857..4948
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagt ttctagacgg agtactgtcc tccgagcgga gtactgtcct ccgactcgag
       61 cggagtactg tcctccgatc ggagtactgt cctccgcgaa ttccggagta ctgtcctccg
      121 aagacgctag cggggggcta taaaaggggg tgggggcgtt cgtcctcact ctagatctgc
      181 gatctaagta agcttggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa
      241 acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac
      301 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
      361 atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag
      421 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
      481 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
      541 acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg
      601 tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca
      661 tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
      721 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct
      781 tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta
      841 aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta
      901 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
      961 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     1021 atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc
     1081 tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt
     1141 tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag
     1201 cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta
     1261 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     1321 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     1381 aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg
     1441 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     1501 gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag
     1561 gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg
     1621 acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg
     1681 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     1741 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     1801 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     1861 gaaagatcgc cgtgtaattc tagagtcggg gcggccggcc gcttcgagca gacatgataa
     1921 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     1981 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     2041 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     2101 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgtcg accgatgccc
     2161 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     2221 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc
     2281 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2341 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2401 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2461 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2521 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2581 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2641 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2701 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2761 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2821 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2881 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2941 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3001 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3061 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3121 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3181 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3241 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3301 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3361 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3421 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3481 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3541 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3601 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3661 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3721 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3781 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3841 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3901 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3961 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4021 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4081 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4141 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4201 gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4261 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4321 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4381 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4441 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4501 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4561 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4621 aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcccatt cgccattcag
     4681 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagcccaa
     4741 gctaccatga taagtaagta atattaaggt acgggaggta cttggagcgg ccgcaataaa
     4801 atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca
     4861 tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt
     4921 gcaagtgcag gtgccagaac atttctctat cgata
//
