LOCUS       40924_20845        4473 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pG5CAT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Holtz A, Lou Y.
  TITLE     pG5CAT complete sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Holtz A, Lou Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-1997) CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303-4230, USA
REFERENCE   3  (bases 1 to 4473)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4473)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-FEB-1997) CLONTECH Laboratories, Inc., 1020 East Meadow Circle, 
            Palo Alto, CA 94303-4230, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an order
            call (415) 424-8222 or (800) 662-2566, extension 1. International 
            customers, please contact your local distributor. For technical 
            information, call (415) 424-8222 or (800) 662-2566, extension 3. 
            This sequence has been compiled from information in the sequence 
            databases, published literature and other sources, together with 
            partial sequences obtained by CLONTECH. If you suspect there is an 
            error in this sequence, please contact CLONTECH's Technical Support 
            Department at (415) 424-8222 or (800) 662-2566, extension 3 or 
            E-mail TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..4473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..776
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     intron          945..1010
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1140..1160
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    1585..1719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1742..1758)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2232..2336
                     /label=AmpR promoter
     CDS             2337..3194
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3368..3956
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4244..4265
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4280..4310
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4318..4334
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4342..4358
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctcgaggaca gtactccgct cggaggacag tactccgatc cgtcgactct agagggtata
       61 taatgcgcca gctcgaattc atcagcttgg cgagattttc aggagctaag gaagctaaaa
      121 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
      181 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
      241 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
      301 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
      361 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
      421 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
      481 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
      541 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
      601 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
      661 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg
      721 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
      781 ttttttaagg cagttattgg tgcccttaaa cgcctggtgc tacgcctgaa taagtgataa
      841 taagcggatg aatggcagaa attcgccgga tctttgtgaa ggaaccttac ttctgtggtg
      901 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
      961 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     1021 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     1081 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     1141 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     1201 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     1261 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
     1321 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
     1381 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
     1441 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     1501 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     1561 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     1621 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     1681 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc ccgggtaccg agctcgaatt
     1741 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     1801 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     1861 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     1921 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     1981 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     2041 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     2101 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     2161 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     2221 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     2281 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     2341 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     2401 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     2461 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     2521 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     2581 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     2641 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     2701 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     2761 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     2821 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     2881 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     2941 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     3001 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     3061 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     3121 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     3181 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     3241 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     3301 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3361 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3421 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3481 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     3541 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3601 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3661 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3721 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3781 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3841 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3901 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3961 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4021 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4081 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4141 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     4201 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     4261 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     4321 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctctatg
     4381 accatgatta cgcgccaagc taattcccgg gatccgctcg gaggacagta ctccgctcgg
     4441 aggacagtac tccgctcgga ggacagtact ccg
//
