LOCUS       62056_11180        5764 bp DNA     circular SYN 03-NOV-2020
DEFINITION  Cloning vector pG20_mVenus_Blp, complete sequence.
ACCESSION   MT896405
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Pratt AI, Knoblauch J, Kunz H-H.
  TITLE     An updated pGREEN-based vector suite for cost-effective cloning in 
            plant molecular biology
  JOURNAL   MicroPubl Biol (2020)
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Pratt AI, Kunz H-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2020) School of Biological Sciences, Washington 
            State University, 1150 NE College Ave #385, Pullman, WA 99163, USA
REFERENCE   3  (bases 1 to 5764)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-AUG-2020) School of Biological Sciences, Washington State 
            University, 1150 NE College Ave #385, Pullman, WA 99163, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      63..498
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    625..647
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     polyA_signal    complement(655..831)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1066..1614)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(1634..1979)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      1989..2622
                     /label=pUBQ10
                     /note="pUBQ10"
                     /regulatory_class="promoter"
     misc_feature    2623..2661
                     /label=MCS
                     /note="MCS"
     CDS             2707..3423
                     /label=Venus
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
     terminator      3434..3682
                     /label=HSP terminator
                     /note="efficient transcription terminator from the
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    3847..3871
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(3962..4550)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4724..5536)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
       61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
      121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
      181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
      241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
      301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
      361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
      421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
      481 ttgcccaccg tcgagcgcct ttgcccacaa cccggcggcc ggccgcaaca gatcgtttta
      541 taaatttttt tttttgaaaa agaaaaagcc cgaaaggcgg caacctctcg ggcttctgga
      601 tttccgatcc ccggaattag atcttggcag gatatattgt ggtgtaacgt tatcgatctg
      661 gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac
      721 aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa accctataag
      781 aaccctaatt tcccttatcg ggaaactact cacacattat ttatggagaa aatagagaga
      841 gatagatttg tagagagaga ctggtgattt cagcgtaccg aattaattcc tgaatcactg
      901 cgaccggccc tcccgcgacc cagccgagcg agcttagcga actgtggacg agaactgtgc
      961 caccaagcgt aaggccgttc tctcgcattt gccttgctag gctcgcgcga gttgctggct
     1021 gaggcgttct cgaaatcagc tcttgttcgg tcggcatcta cttcagattt cggtgacggg
     1081 caggaccgga cggggcggca ccggcaggct gaagtccagc tgccagaaac ccacgtcatg
     1141 ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat atccgagcgc
     1201 ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca cgctcttgaa
     1261 gccctgtgcc tccagggact tcagcaggtg ggtgtagagc gtggagccca gtcccgtccg
     1321 ctggtggcgg ggggagacgt acacggtgga ctcggccgtc cagtcgtagg cgttgcgtgc
     1381 cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg cgacgagcca
     1441 gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt cctgcggctc
     1501 ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc agaccgccgg
     1561 catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc tcataggggg
     1621 gatcagcttg ggctgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt
     1681 gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatgtca catcaatcca
     1741 cttgctttga agtcgtggtt ggaacgactt ctttttccac gatgctcctc gtgggtgggg
     1801 gtccatcttt gggaccactg tcggcagagg catcttgaat gatagccttt cctttatcgc
     1861 aatgatggca tttgtaggag ccaccttcct tttctactgt cctttcgatg aagtgacaga
     1921 tagctgggca atggaatccg aggaggtttc ccgaaattac cctttgttga aaagtctcaa
     1981 taaagcttcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag
     2041 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc
     2101 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt
     2161 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct
     2221 ataaaacaat acccaaagac ctcttcttct tcacaattca gatttcaatt tctcaaaatc
     2281 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt
     2341 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt
     2401 agattctgtt aatcttagat cgaagtcgat tttctgggtt tgatcgttag atatcatctt
     2461 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc
     2521 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg
     2581 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac agactagagt taaccgggct
     2641 caggcctggc gcgccactag tggatccatc gatagtactg tcgacctcga gggtaccgct
     2701 cccgggatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2761 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     2821 acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg
     2881 cccaccctcg tgaccaccct gggctacggc ctgcagtgct tcgcccgcta ccccgaccac
     2941 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3001 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     3061 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     3121 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag
     3181 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
     3241 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     3301 aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     3361 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     3421 aagtaagagc tcatatgaag atgaagatga aatatttggt gtgtcaaata aatagcttgt
     3481 gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg ctttttctaa
     3541 tattaaatga atgtaagatc tcattataat gaataaacaa atgtttctat aatccattgt
     3601 gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat ggtatggact
     3661 atggaatatg attaaagata aggaattcag atcggctgag tggctccttc aacgttgcgg
     3721 ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg
     3781 actcccttaa ttctccgctc atgatcagat tgtcgtttcc cgccttcagt ttaaactatc
     3841 agtgtttgac aggatatatt ggcgggtaaa ctaagtcgct gtatgtgttt gtttgagatc
     3901 tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3961 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4021 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4081 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4141 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4201 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4261 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4321 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4381 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     4441 ccttcggaag aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4501 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4561 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4621 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4681 aatcaatcta aagtatatat gtgtaacatt ggtctagtga ttagaaaaac tcatcgagca
     4741 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     4801 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     4861 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     4921 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     4981 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     5041 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     5101 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     5161 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     5221 ctgttttccc tgggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     5281 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     5341 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     5401 tcccatacaa tcggtagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     5461 acccatataa atcagcatcc atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc
     5521 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     5581 ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt
     5641 ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg
     5701 acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca
     5761 acaa
//
