LOCUS       62056_11190        5077 bp DNA     circular SYN 03-NOV-2020
DEFINITION  Cloning vector pG20_Myc_Blp, complete sequence.
ACCESSION   MT896417
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Pratt AI, Knoblauch J, Kunz H-H.
  TITLE     An updated pGREEN-based vector suite for cost-effective cloning in 
            plant molecular biology
  JOURNAL   MicroPubl Biol (2020)
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Pratt AI, Kunz H-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2020) School of Biological Sciences, Washington 
            State University, 1150 NE College Ave #385, Pullman, WA 99163, USA
REFERENCE   3  (bases 1 to 5077)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-AUG-2020) School of Biological Sciences, Washington State 
            University, 1150 NE College Ave #385, Pullman, WA 99163, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..375
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             395..943
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     polyA_signal    1178..1354
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(1362..1384)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     rep_origin      complement(1511..1946)
                     /direction=LEFT
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     CDS             2237..3049
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3223..3811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3902..3926)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(4091..4339)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(4350..4379)
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     misc_feature    complement(4380..4457)
                     /label=MCS
                     /note="MCS"
     regulatory      complement(4464..5077)
                     /label=pUBQ10
                     /note="pUBQ10"
                     /regulatory_class="promoter"
ORIGIN
        1 cgtttattac tgactcgtcg aagctttatt gagacttttc aacaaagggt aatttcggga
       61 aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag
      121 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggctatcat tcaagatgcc
      181 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
      241 gtcgttccaa ccacgacttc aaagcaagtg gattgatgtg acatctccac tgacgtaagg
      301 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
      361 catttggaga ggacagccca agctgatccc ccctatgagc ccagaacgac gcccggccga
      421 catccgccgt gccaccgagg cggacatgcc ggcggtctgc accatcgtca accactacat
      481 cgagacaagc acggtcaact tccgtaccga gccgcaggaa ccgcaggagt ggacggacga
      541 cctcgtccgt ctgcgggagc gctatccctg gctcgtcgcc gaggtggacg gcgaggtcgc
      601 cggcatcgcc tacgcgggtc cctggaaggc acgcaacgcc tacgactgga cggccgagtc
      661 caccgtgtac gtctcccccc gccaccagcg gacgggactg ggctccacgc tctacaccca
      721 cctgctgaag tccctggagg cacagggctt caagagcgtg gtcgctgtca tcgggctgcc
      781 caacgacccg agcgtgcgca tgcacgaggc gctcggatat gccccccgcg gcatgctgcg
      841 ggcggccggc ttcaagcacg ggaactggca tgacgtgggt ttctggcagc tggacttcag
      901 cctgccggtg ccgccccgtc cggtcctgcc cgtcaccgaa atctgaagta gatgccgacc
      961 gaacaagagc tgatttcgag aacgcctcag ccagcaactc gcgcgagcct agcaaggcaa
     1021 atgcgagaga acggccttac gcttggtggc acagttctcg tccacagttc gctaagctcg
     1081 ctcggctggg tcgcgggagg gccggtcgca gtgattcagg aattaattcg gtacgctgaa
     1141 atcaccagtc tctctctaca aatctatctc tctctatttt ctccataaat aatgtgtgag
     1201 tagtttcccg ataagggaaa ttagggttct tatagggttt cgctcatgtg ttgagcatat
     1261 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
     1321 cctaaaacca aaatccagta ctaaaatcca gatcgataac gttacaccac aatatatcct
     1381 gccaagatct aattccgggg atcggaaatc cagaagcccg agaggttgcc gcctttcggg
     1441 ctttttcttt ttcaaaaaaa aaaatttata aaacgatctg ttgcggccgg ccgccgggtt
     1501 gtgggcaaag gcgctcgacg gtgggcaacc gcttgcggtt gtccacgggc ggagccggtg
     1561 cgcgtagcgc attgtccaca agccaagggc gaccaataat tgatatatat attcataatt
     1621 gaaaagctaa ttgaacatac tacttgctgt aactacttgc cggagcgagg ggtgtttgca
     1681 agctgttgat ctgaaagggc tattagcgtt ctcacgtgcc tttttgatta gcgatttcac
     1741 gtgaccttat tagcgatttc acgtactccg attagcgatt tcacgtaccc tgattagcga
     1801 tttcacgtgg atagtttttg gagcgggccg gaaagccccg tgaatcaagg ctttgcgggg
     1861 cattagcggt ttcacgtgga taactaccct ctatccacag gcttccgggg ataaaaaagc
     1921 ccgctcgacg gcgggctgtt ggatggggat cgcctgaatc gccccatcat ccagccagaa
     1981 agtgagggag ccacggttga tgagagcttt gttgtaggtg gaccagttgg tgattttgaa
     2041 cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa
     2101 ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc tctgatgtta
     2161 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     2221 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcaa ggccgcgatt
     2281 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     2341 atcaggtgcg acaatctacc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa
     2401 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     2461 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     2521 gttactcacc actgcgatcc cagggaaaac agcattccag gtattagaag aatatcctga
     2581 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     2641 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     2701 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     2761 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
     2821 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     2881 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     2941 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     3001 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcactaga
     3061 ccaatgttac acatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3121 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3181 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3241 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3301 ggatcaagag ctaccaactc ttcttccgaa ggtaactggc ttcagcagag cgcagatacc
     3361 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3421 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3481 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3541 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3601 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3661 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3721 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3781 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3841 cctggccttt tgctggcctt ttgctcacat gagatctcaa acaaacacat acagcgactt
     3901 agtttacccg ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga
     3961 caatctgatc atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg
     4021 gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg
     4081 atctgaattc cttatcttta atcatattcc atagtccata ccatagcaca tacagtagtt
     4141 atatgctgca gaagagatcc aacaaaacat tcacaatgga ttatagaaac atttgtttat
     4201 tcattataat gagatcttac attcatttaa tattagaaaa agccacaaat tcataacaca
     4261 acaagccaag aaaaaaacac aaacttaagc acacaagcta tttatttgac acaccaaata
     4321 tttcatcttc atcttcatat gagctcttac aagtcttcct ccgagatcag cttctgctcc
     4381 ccgggagcgg taccctcgag gtcgacagta ctatcgatgg atccactagt ggcgcgccag
     4441 gcctgagccc ggttaactct agtctgttaa tcagaaaaac tcagattaat cgacaaattc
     4501 gatcgcacaa actagaaact aacaccagat ctagatagaa atcacaaatc gaagagtaat
     4561 tattcgacaa aactcaaatt atttgaacaa atcggatgat atctatgaaa ccctaatcga
     4621 gaattaagat gatatctaac gatcaaaccc agaaaatcga cttcgatcta agattaacag
     4681 aatctaaacc aaagaacata tacgaaattg ggatcgaacg aaaacaaaat cgaagatttt
     4741 gagagaataa ggaacacaga aatttacctt gatcacggta gagagaattg agagaaagtt
     4801 tttaagattt tgagaaattg aaatctgaat tgtgaagaag aagaggtctt tgggtattgt
     4861 tttatagaag aagaagaaga aaagacgagg acgactaggt cacgagaaag ctaaggcggt
     4921 gaagcaatag ctaataataa aatgacacgt gtattgagcg ttgtttacac gcaaagttgt
     4981 ttttggctaa ttgccttatt tttaggttga ggaaaagtat ttgtgctttg agttgataaa
     5041 cacgactcgt gtgtgccggc tgcaaccact ttgacgc
//
