LOCUS       62056_11170        5002 bp DNA     circular SYN 03-NOV-2020
DEFINITION  Cloning vector pG20_MCS_blp, complete sequence.
ACCESSION   MT896411
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Pratt AI, Knoblauch J, Kunz H-H.
  TITLE     An updated pGREEN-based vector suite for cost-effective cloning in 
            plant molecular biology
  JOURNAL   MicroPubl Biol (2020)
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Pratt AI, Kunz H-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2020) School of Biological Sciences, Washington 
            State University, 1150 NE College Ave #385, Pullman, WA 99163, USA
REFERENCE   3  (bases 1 to 5002)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-AUG-2020) School of Biological Sciences, Washington State 
            University, 1150 NE College Ave #385, Pullman, WA 99163, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      63..498
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    625..647
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     polyA_signal    complement(655..831)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1066..1614)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(1634..1979)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      1989..2622
                     /label=pUBQ10
                     /note="pUBQ10"
                     /regulatory_class="promoter"
     misc_feature    2623..2661
                     /label=MCS
                     /note="MCS"
     terminator      2672..2920
                     /label=HSP terminator
                     /note="efficient transcription terminator from the
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    3085..3109
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(3200..3788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3962..4774)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
       61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
      121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
      181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
      241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
      301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
      361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
      421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
      481 ttgcccaccg tcgagcgcct ttgcccacaa cccggcggcc ggccgcaaca gatcgtttta
      541 taaatttttt tttttgaaaa agaaaaagcc cgaaaggcgg caacctctcg ggcttctgga
      601 tttccgatcc ccggaattag atcttggcag gatatattgt ggtgtaacgt tatcgatctg
      661 gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac
      721 aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa accctataag
      781 aaccctaatt tcccttatcg ggaaactact cacacattat ttatggagaa aatagagaga
      841 gatagatttg tagagagaga ctggtgattt cagcgtaccg aattaattcc tgaatcactg
      901 cgaccggccc tcccgcgacc cagccgagcg agcttagcga actgtggacg agaactgtgc
      961 caccaagcgt aaggccgttc tctcgcattt gccttgctag gctcgcgcga gttgctggct
     1021 gaggcgttct cgaaatcagc tcttgttcgg tcggcatcta cttcagattt cggtgacggg
     1081 caggaccgga cggggcggca ccggcaggct gaagtccagc tgccagaaac ccacgtcatg
     1141 ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat atccgagcgc
     1201 ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca cgctcttgaa
     1261 gccctgtgcc tccagggact tcagcaggtg ggtgtagagc gtggagccca gtcccgtccg
     1321 ctggtggcgg ggggagacgt acacggtgga ctcggccgtc cagtcgtagg cgttgcgtgc
     1381 cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg cgacgagcca
     1441 gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt cctgcggctc
     1501 ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc agaccgccgg
     1561 catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc tcataggggg
     1621 gatcagcttg ggctgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt
     1681 gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatgtca catcaatcca
     1741 cttgctttga agtcgtggtt ggaacgactt ctttttccac gatgctcctc gtgggtgggg
     1801 gtccatcttt gggaccactg tcggcagagg catcttgaat gatagccttt cctttatcgc
     1861 aatgatggca tttgtaggag ccaccttcct tttctactgt cctttcgatg aagtgacaga
     1921 tagctgggca atggaatccg aggaggtttc ccgaaattac cctttgttga aaagtctcaa
     1981 taaagcttcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag
     2041 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc
     2101 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt
     2161 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct
     2221 ataaaacaat acccaaagac ctcttcttct tcacaattca gatttcaatt tctcaaaatc
     2281 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt
     2341 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt
     2401 agattctgtt aatcttagat cgaagtcgat tttctgggtt tgatcgttag atatcatctt
     2461 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc
     2521 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg
     2581 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac agactagtgg atccgtcgac
     2641 ctcgagggta ccgctcccgg gtaggagctc atatgaagat gaagatgaaa tatttggtgt
     2701 gtcaaataaa tagcttgtgt gcttaagttt gtgttttttt cttggcttgt tgtgttatga
     2761 atttgtggct ttttctaata ttaaatgaat gtaagatctc attataatga ataaacaaat
     2821 gtttctataa tccattgtga atgttttgtt ggatctcttc tgcagcatat aactactgta
     2881 tgtgctatgg tatggactat ggaatatgat taaagataag gaattcagat cggctgagtg
     2941 gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc
     3001 ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcagattg tcgtttcccg
     3061 ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaact aagtcgctgt
     3121 atgtgtttgt ttgagatctc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3661 ctctgctgaa gccagttacc ttcggaagaa gagttggtag ctcttgatcc ggcaaacaaa
     3721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3901 attaaaaatg aagttttaaa tcaatctaaa gtatatatgt gtaacattgg tctagtgatt
     3961 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     4021 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     4081 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     4141 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     4201 aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc
     4261 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
     4321 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
     4381 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
     4441 cttctaatac ctggaatgct gttttccctg ggatcgcagt ggtgagtaac catgcatcat
     4501 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
     4561 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
     4621 actctggcgc atcgggcttc ccatacaatc ggtagattgt cgcacctgat tgcccgacat
     4681 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     4741 ttgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     4801 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
     4861 gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc
     4921 agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca
     4981 ccaactggtc cacctacaac aa
//
