LOCUS       V006137                10344 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006137
VERSION     V006137
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Jones KR, Macrina FL, Lewis JP.
  TITLE     Porphyromonas gingivalis shuttle vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10344)
  AUTHORS   Jones KR, Macrina FL, Lewis JP.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2017) OCMB, Virginia Commonwealth University,
            Philips Institute, 521 North 11th Street, Richmond, VA 23298, USA
REFERENCE   3  (bases 1 to 10344)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10344)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-JUL-2017) OCMB, Virginia Commonwealth University, Philips
            Institute, 521 North 11th Street, Richmond, VA 23298, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     688..704
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    705..761
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(774..790)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    798..814
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(822..852)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(867..888)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     RBS             1448..1456
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1899..3491
                     /codon_start=1
                     /gene="repB"
                     /product="RepB"
                     /label="repB"
                     /protein_id="AVN57612.1"
                     /translation="MRTFEYRSLKPFEFFQIIFGSCDHNDPQCPIYPFEKELCQEYIDW
                     RAQQAGRKSKSKAGKKAIYNPAQPFRYNALVQAVYSVEPDAGRANDNGRRSKTWVVVGD
                     ATSEDDIKVCGWDSLEELMGRYKFVVTTPATYVGRRRFKSNARYLYALAFDLDEVGVPE
                     MSEVIHQQTIGMTPQANIIVNSGDGIHLYYILAKPMPLYPKVYETLTNIKKALTRLIWN
                     KASSRTGEDNQVQIQPIIQPFRVPGSRTKHGDIVTAWHNADAPLHTIEELNRFASKPTL
                     KYLNSGVSQEQAQAFGQRGILPRIWLTKARAKELYPDWYQKRIVEGAPRKTWHVHRGLY
                     EWWLHMLWTNEKVAVGHRYHCIMFLAVYAKKCNVPFDILKRDALELVPRMEALTGNTGR
                     HFSVQDALDALKAYKESSETYPRQLIEDRSGLRIEPNKRNGRPQDLHLKLARGSRDILQ
                     EAKGRKDWREGNGRPKGSIVLAEDSPQYAKVQEWRDRNPNSNNKSRCARETGLSRPTRP
                     QVVAKPLGNALRT"
     gene            1899..3491
                     /gene="repB"
                     /label="repB"
     CDS             complement(3650..4627)
                     /codon_start=1
                     /gene="mob"
                     /product="Mob"
                     /label="mob"
                     /note="plasmid stability"
                     /protein_id="AVN57616.1"
                     /translation="MSKTSIHIEPCKIGSSEQHNQRLKHLDYVRPDLSPQNESWVGVAD
                     LPAHLEQLRILVKEKTGRAMQQKATPIREGVIVINQGTSINQLRGLATAIELRWGIKTL
                     QIYTHKDEGHIDSDDTWKPNLHAHMVLDWVNHDTGKSIKLSKQDMAEMQTMVADCLQMV
                     RGESSDVKHLGAIQYKNQAEELRLQTLKEQTMKEEQARELALAEAKRMEQVASEQIIAE
                     NKKLGGLWMDWQKSFEASEKRRESITARKIEEDKLWEELGLKGAFKSFWEECKDWFRGF
                     GDGLKDLALLIWASPRGVMDKDVRCTADRKRGKLLLKWAYTRSR"
     gene            complement(3650..4627)
                     /gene="mob"
                     /label="mob"
     CDS             complement(5642..6175)
                     /codon_start=1
                     /product="hypothetical protein"
                     /label="hypothetical protein"
                     /note="orfE"
                     /protein_id="AVN57613.1"
                     /translation="MLVRGAEPMEKRQQRGLFTVPGLLLAFCSHGSSIADGLVAHPSEG
                     FYIIYTTSSEEFLTLSEEDYSLFASFANLVVREDSNSNGLRSAKPSGDGWKFGGKGKGK
                     LDAIHIGYELSKILGENQDFEIYVEYNGDGSYTVWYREVQKSSKGSNPVKTFNVLIRAN
                     EREGHTVSLFQPYA"
     rep_origin      complement(6145..6733)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7074..7808)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     CDS             8314..10236
                     /gene="tetQ"
                     /label="Tetracycline resistance protein TetQ"
                     /note="Tetracycline resistance protein TetQ from
                     Bacteroides fragilis. Accession#: Q08425"
ORIGIN
        1 gctttctctc actctgaatg gataaagttt gctatctttg ctttaattgt ccgtccaaag
       61 aaaaaaaagt tgcagatgag caaacatata accgaggaac aaaggtatgc aatttctatg
      121 atgttgcaaa taccgatgag caaaaaagca atagcggaag ctatcggggg taccgagctc
      181 gaattattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
      241 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
      301 cgtcttagct ctgctgatat gggcttcgcc tagaggagtg atggactccc tcccggagac
      361 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
      421 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
      481 agtgcaccat cntgatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
      541 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
      601 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
      661 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta
      721 cccggggatc ctctagagtc gacctgcagg catgcaagct tggcgtaatc atggtcatag
      781 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      841 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      901 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      961 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     1021 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1081 ttatccacag aatcagggga taacgcagga aagaacatga gctcggtacc cggatccaat
     1141 tgagtgattt gttctggttc agtgtcttgg cttggcttta acaagtcaag gttgttttgg
     1201 cacgaagtta acgccaaaga caatagcccg attgctattg cgcagaatgt tctagttttc
     1261 atagctgctt aggtttagtg ttttgtattc aagataagtc tctcttaaga ggttgtctta
     1321 ccgcaaagat aaagaattgc aaaaaatata tgcaatacct acttgttgcg atattctttg
     1381 tttcttcccc aaaaaatcaa ccccaaaatg accgtagaac gatgacgcca gaaggaggtc
     1441 tcaaaaaacc tccttacctt tgtaccacaa gtggggattt tacttcattg ataatatacg
     1501 cccccccgta tgaatttttg gggagcgtag cgagccaaaa attccaccca aaatgacgta
     1561 gaaggaggtc tcaaaaatcc tccttatctt tgtgccacaa gtggcaaaaa aaaatatatt
     1621 gataatatat gccccccgta tgattttttg ggggagcgaa gcgacaccaa aaaatcaccc
     1681 caaaatgacg tagaaggagg gtctcaaaaa tcctccttat ctttgtgcca caagtggcaa
     1741 aaaaaatata ttgataatat atgccccccc gtatgatttt ttgggggagc gaagcgacac
     1801 ccaaaaatca ccccaaaatg gacgttagaa cggagactga taattctaat atttctcacg
     1861 atggtttttc tctctaaaaa tttatactta gagaggtaat gagaaccttt gaatatcgat
     1921 ctcttaaacc ctttgagttc ttccaaataa tctttggttc ttgtgaccat aatgacccgc
     1981 aatgccctat ctatcctttt gaaaaagagc tgtgccaaga atacatcgat tggagggctc
     2041 aacaagcagg gagaaagtct aagtccaaag caggtaagaa agcaatctat aatccagctc
     2101 aacctttccg ctataatgct ttggtgcaag ctgtctacag cgtagagcct gatgcaggca
     2161 gggctaatga taatggcagg aggtctaaga cgtgggtggt agtgggcgat gccacaagcg
     2221 aggatgacat caaggtgtgt ggttgggata gcttagagga gctgatgggg cggtacaagt
     2281 ttgtggtaac aaccccagcc acctacgtgg ggcgcaggag gttcaagagc aatgcccgct
     2341 atctgtatgc cctcgccttt gaccttgatg aggtcggagt gccagagatg tcagaggtaa
     2401 tccatcagca gacgataggg atgaccccac aagctaatat catcgtcaac tcgggcgatg
     2461 gcatccactt gtactacata cttgctaagc cgatgccctt ataccccaag gtgtacgaga
     2521 cgctcaccaa cattaaaaaa gctcttacac ggcttatatg gaacaaagcg tcctctagga
     2581 cgggggagga caaccaagtg caaatacagc ccattataca gccgtttagg gtgccaggca
     2641 gtcgtacgaa gcacggggac attgtaacag cttggcacaa tgctgatgcc ccactacaca
     2701 ctattgagga gctgaatcgg tttgctagca agcctacact gaagtacctt aactcggggg
     2761 tgtcgcagga gcaggcgcaa gcgtttggac aaaggggcat actcccacga atatggctaa
     2821 caaaagcaag agctaaagag ctatacccag attggtatca aaaacgcatc gtagagggcg
     2881 cacctcgtaa gacgtggcat gtgcataggg ggctgtatga gtggtggcta catatgctgt
     2941 ggacgaacga aaaggtcgct gttgggcatc gctaccactg cattatgttc ttagcagtat
     3001 atgccaagaa gtgtaacgtg ccatttgaca tactcaaacg ggacgctcta gagcttgtgc
     3061 cacgaatgga agcactaacg ggcaacacgg gcaggcactt ctccgtgcag gacgctctag
     3121 acgctctaaa agcatataag gagagctcag agacataccc tcgccagctg atagaggata
     3181 ggtcggggct aagaatagaa cctaacaaac gcaatgggag accgcaagac ttacatctaa
     3241 agctcgcaag aggctctaga gatattttgc aggaagcaaa gggaagaaaa gattggagag
     3301 agggtaacgg aagaccaaaa ggctctatcg tactagcgga agacagcccg caatacgcca
     3361 aggtgcagga gtggagggat agaaacccaa acagcaacaa caagagccgt tgtgcgagag
     3421 agacaggact aagtagaccg acccgtccgc aagtggtggc aaagcccttg ggaaacgctt
     3481 taagaacata gtgcggtcaa atcttgcgac ccctgcgctc tcgtctctct tggtcaacac
     3541 gcttctctct gcgtctgtct tgtatctcta gcctgcctcg tagcgcactc actgacggat
     3601 tgcgcctgcc tttgtatggg ggagcttggt acaactatct tgggtgctct tacctgcttc
     3661 tagtgtatgc ccatttgagc agtaatttcc ccctcttccg atccgctgtg cacctcacgt
     3721 ctttgtccat cactcctcta ggcgaagccc atatcagcag agctaagtct ttgagcccat
     3781 cgccgaaacc tctaaaccaa tccttgcatt cctcccaaaa cgatttaaag gctcctttca
     3841 acccaagttc ttcccatagc ttatcctcct ctatcttacg agccgttatg ctctctcggc
     3901 gcttctcaga ggcctcaaat gatttttgcc agtccatcca cagccctcca agcttcttat
     3961 tctctgcgat aatctgctca gacgcaacct gctccatccg cttagcttcg gctagtgcca
     4021 gctcccgagc ctgttcttct ttcatcgttt gttctttaag ggtttggaga cgcagctctt
     4081 cggcttgatt cttgtattgt atagccccga ggtgtttgac atcgctactc tctcccctaa
     4141 ccatttgcag gcagtccgcc accatcgtct gcatctccgc catatcttgc ttgctaagct
     4201 tgatactctt gcccgtgtcg tggttcaccc aatcaagcac catatgggca tgtaggtttg
     4261 gcttccacgt gtcatcgcta tctatatgcc cctcgtcctt gtgggtgtat atctgtagcg
     4321 tcttaatacc ccatcgcagt tctattgccg ttgcgagacc ccttaattgg ttaatcgagg
     4381 tgccttgatt gattactatt acaccctctc ttatcggggt tgctttctgt tgcatcgctc
     4441 tgcccgtctt ctcctttaca agtattcgga gttgttcaag atgcgcgggc aaatctgcta
     4501 cgccaaccca actctcgttt tgtggggaga ggtcgggtcg cacataatca agatgcttca
     4561 agcgttgatt atgttgctca ctactgccga ttttacacgg ctcgatatgg atacttgttt
     4621 tgctcatcgc ttgtactttt gggtcaactt tagggggtgc tgggggtgtc ctccatgccc
     4681 ctcggagagc acacaactac ggtatgtagt tataatgtgc tataactatg gtcgtagcta
     4741 gcgcctgccc aaagttacgg agaacgattt tattggacaa cttcttgtcg ctcccattac
     4801 acacacattt ggtgcgtgaa ctgacgccca gccgacagac gtttatttgg gagatttgtc
     4861 tatatttgca gtgaatataa tcaagtagaa tataatagaa atggataata ttgagctaaa
     4921 agcatccacg tcaattagaa attgatgcta gtgtactcca gcagatacgc agtgaagcaa
     4981 gggctgagaa tagaagccta agcaactata ttgagacctt actctaccga atggggtata
     5041 gaccttataa tgccgagacg gcagaggcct gcatagacgc taagagcggt cgctatgccg
     5101 gctctgtgga tacaactacc cgagaaacaa tagaagcgtc tttgttttgg aaatgaggaa
     5161 gcgtaggctg gtatatacta ccaagtttaa gaaagacctt aagcgattta gaaacgacct
     5221 caaaaggcgc tctaaaatcg tagatgtcat tgaaatgctg tctaagggtg tggagatacc
     5281 cttagaaatg cgtcctcaca agcttatagg gaactatgag ggctgtatgg agctacacat
     5341 tgagggtgac ctgctcttga tatggataga gcagataagc gcagacgagg agcttatcgt
     5401 cctctcacgc ttaggctcgc acgcagatct cttttagtaa ctttggcaag tgagatagag
     5461 ctactaagta gcgccacggt gatcctctga catcgttatg gttcgtggct ttgggcttag
     5521 tagctctatt tctgttatgt catatccatg gtatttgcct atcatatttt caaaacgaaa
     5581 aacagaaata gagctgacct atcaagccag ctctatttaa tcacatcgag taagcaatcg
     5641 ttcaagcata cggttgaaag agagagacgg tatgcccctc tctttcgttg gctcttatga
     5701 ggacattaaa agtctttacg ggattactcc ctttagacga tttttggact tccctatacc
     5761 aaacggtata agacccatcg ccattgtatt ctacatagat ctcaaaatct tgattttctc
     5821 ctaggatttt cgataattca tagccgatgt ggatggcatc tagtttcccc tttcctttac
     5881 ctccaaattt ccagccatcc ccagagggtt ttgcgcttct taagccattt gaattggagt
     5941 cctctcgaac aaccaaattg gcaaaagaag cgaacagaga ataatcttct tcagataacg
     6001 tgagaaactc ctctgaagag gtggtataga tgatatagaa cccttcagag gggtgtgcaa
     6061 caagaccatc tgcaatagag gatccatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6121 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6181 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6241 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6301 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6361 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6421 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6481 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6541 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     6601 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6661 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6721 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6781 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6841 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6901 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6961 catagttgcc tgactcgatc cccgaagctg tcagtagtat acctaataat ttatctacat
     7021 tccctttagt aacgtgtaac tttccaaatt tacaaaagcg actcatagaa ttatttcctc
     7081 ccgttaaata atagataact attaaaaata gacaatactt gctcataagt aacggtactt
     7141 aaattgttta ctttggcgtg tttcattgct tgatgaaact gatttttagt aaacagttga
     7201 cgatattctc gattgaccca ttttgaaaca aagtacgtat atagcttcca atatttatct
     7261 ggaacatctg tggtatggcg ggtaagtttt attaagacac tgtttacttt tggtttagga
     7321 tgaaagcatt ccgctggcag cttaagcaat tgctgaatcg agacttgagt gtgcaagagc
     7381 aaccctagtg ttcggtgaat atccaaggta cgcttgtaga atccttcttc aacaatcaga
     7441 tagatgtcag acgcatggct ttcaaaaacc acttttttaa taatttgtgt gcttaaatgg
     7501 taaggaatac tcccaacaat tttatacctc tgtttgttag ggaattgaaa ctgtagaata
     7561 tcttggtgaa ttaaagtgac acgagtattc agttttaatt tttctgacga taagttgaat
     7621 agatgactgt ctaattcaat agacgttacc tgtttactta ttttagccag tttcgtcgtt
     7681 aaatgccctt tacctgttcc aatttcgtaa acggtatcgg tttcttttaa attcaattgt
     7741 tttattattt ggttgagtac tttttcactc gttaaaaagt tttgagaata ttttatattt
     7801 ttgttcatgt aatcactcct tcttaattac aaatttttag catctaattt aacttcaatt
     7861 cctattatac aaaattttaa gatactgcac tatcaacaca ctcttaagtt tgcttctaag
     7921 tcttatttcc ataacttctt ttacgtttcc gctccatcgc caatttgcca gccgttatgc
     7981 ggcagcttaa aaaaagacga caccaaacca aaaaaatcat cttgacaacc acccgacttt
     8041 gaactacgaa ggatgaaatt tttcagggac aacttccagc atttaccagt tgaacctacg
     8101 tttccctaat aaaatgtcta tggtaaaaag ttaaaaaatc ctcctacttt tgttagatat
     8161 atttttttgt gtaattttgt aatcgttatg cggcagtaat aatatacata ttaatacgag
     8221 ttaggaatcc tgtagttctc atatgctacg aggaggtatt aaaaggtgcg tttcgacaat
     8281 gcatctattg tagtatatta ttgcttaatc caaatgaata ttataaattt aggaattctt
     8341 gctcacattg atgcaggaaa aacttccgta accgagaatc tgctgtttgc cagtggagca
     8401 acggaaaagt gcggctgtgt ggataatggt gacaccataa cggactctat ggatatagag
     8461 aaacgtagag gaattactgt tcgggcttct acgacatcta ttatctggaa tggtgtgaaa
     8521 tgcaatatca ttgacactcc gggacacatg gattttattg cggaagtgga gcggacattc
     8581 aaaatgcttg atggagcagt cctcatctta tccgcaaagg aaggcataca agcgcagaca
     8641 aagttgctgt tcaatacttt acagaagctg caaatcccga caattatatt tatcaataag
     8701 attgaccgag ccggtgtgaa tttggagcgt ttgtatctgg atataaaagc aaatctgtct
     8761 caagatgtcc tgtttatgca aaatgttgtc gatggatcgg tttatccggt ttgctcccaa
     8821 acatatataa aggaagaata caaagaattt gtatgcaacc atgacgacaa tatattagaa
     8881 cgatatttgg cggatagcga aatttcaccg gctgattatt ggaatacgat aatcgctctt
     8941 gtggcaaaag ccaaagtcta tccggtgcta catggatcag caatgttcaa tatcggtatc
     9001 aatgagttgt tggacgccat cacttctttt atacttcctc cggcatcggt ctcaaacaga
     9061 ctttcatctt atctttataa gatagagcat gaccccaaag gacataaaag aagttttcta
     9121 aaaataattg acggaagtct gagacttcga gacgttgtaa gaatcaacga ttcggaaaaa
     9181 ttcatcaaga ttaaaaatct aaaaactatc aatcagggca gagagataaa tgttgatgaa
     9241 gtgggcgcca atgatatcgc gattgtagag gatatggatg attttcgaat cggaaattat
     9301 ttaggtgctg aaccttgttt gattcaagga ttatcgcatc agcatcccgc tctcaaatcc
     9361 tccgtccggc cagacaggcc cgaagagaga agcaaggtga tatccgctct gaatacattg
     9421 tggattgaag acccgtcttt gtccttttcc ataaactcat atagtgatga attggaaatc
     9481 tcgttatatg gtttaaccca aaaggaaatc atacagacat tgctggaaga acgattttcc
     9541 gtaaaggtcc attttgatga gatcaagact atatacaaag aacgacctgt aaaaaaggtc
     9601 aataagatta ttcagatcga agtgccgccc aacccttatt gggccacaat agggctgact
     9661 cttgaaccct taccgttagg gacagggttg caaatcgaaa gtgacatctc ctatggttat
     9721 ctgaaccatt cttttcaaaa tgccgttttt gaagggattc gtatgtcttg ccaatccggg
     9781 ttacatggat gggaagtgac tgatctgaaa gtaactttta ctcaagccga gtattatagc
     9841 ccggtaagta cacctgctga tttcagacag ctgacccctt atgtcttcag gctggccttg
     9901 caacagtcag gtgtggacat tctcgaaccg atgctctatt ttgagttgca gataccccaa
     9961 gcggcaagtt ccaaagctat tacagatttg caaaaaatga tgtctgagat tgaagacatc
    10021 agttgcaata atgagtggtg tcatattaaa gggaaagttc cattaaatac aagtaaagac
    10081 tatgcatcag aagtaagttc atacactaag ggcttaggca tttttatggt taagccatgc
    10141 gggtatcaaa taacaaaagg cggttattct gataatatcc gcatgaacga aaaagataaa
    10201 cttttattca tgttccaaaa atcaatgtca tcaaaataat ggagcggtca ggaaatttct
    10261 ataaggcaat acagttggga tatatactta tctccattct tatcggatgt atggcatata
    10321 atagcctcta tgaatggcag gaga
//
