LOCUS       V006138                 9278 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006138
VERSION     V006138
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Jones KR, Macrina FL, Lewis JP.
  TITLE     Porphyromonas gingivalis shuttle vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9278)
  AUTHORS   Jones KR, Macrina FL, Lewis JP.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2017) OCMB, Virginia Commonwealth University,
            Philips Institute, 521 North 11th Street, Richmond, VA 23298, USA
REFERENCE   3  (bases 1 to 9278)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9278)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-JUL-2017) OCMB, Virginia Commonwealth University, Philips
            Institute, 521 North 11th Street, Richmond, VA 23298, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             316..324
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             767..2359
                     /codon_start=1
                     /gene="repB"
                     /product="RepB"
                     /label="repB"
                     /note="putative protein required for plasmid replication in
                     Porphyromonas gingivalis"
                     /protein_id="AVN57617.1"
                     /translation="MRTFEYRSLKPFEFFQIIFGSCDHNDPQCPIYPFEKELCQEYIDW
                     RAQQAGRKSKSKAGKKAIYNPAQPFRYNALVQAVYSVEPDAGRANDNGRRSKTWVVVGD
                     ATSEDDIKVCGWDSLEELMGRYKFVVTTPATYVGRRRFKSNARYLYALAFDLDEVGVPE
                     MSEVIHQQTIGMTPQANIIVNSGDGIHLYYILAKPMPLYPKVYETLTNIKKALTRLIWN
                     KASSRTGEDNQVQIQPIIQPFRVPGSRTKHGDIVTAWHNADAPLHTIEELNRFASKPTL
                     KYLNSGVSQEQAQAFGQRGILPRIWLTKARAKELYPDWYQKRIVEGAPRKTWHVHRGLY
                     EWWLHMLWTNEKVAVGHRYHCIMFLAVYAKKCNVPFDILKRDALELVPRMEALTGNTGR
                     HFSVQDALDALKAYKESSETYPRQLIEDRSGLRIEPNKRNGRPQDLHLKLARGSRDILQ
                     EAKGRKDWREGNGRPKGSIVLAEDSPQYAKVQEWRDRNPNSNNKSRCARETGLSRPTRP
                     QVVAKPLGNALRT"
     gene            767..2359
                     /gene="repB"
                     /label="repB"
     CDS             complement(2518..3495)
                     /codon_start=1
                     /gene="mob"
                     /product="Mob"
                     /label="mob"
                     /note="putative protein required for plasmid stability in
                     Porphyromonas gingivalis"
                     /protein_id="AVN57618.1"
                     /translation="MSKTSIHIEPCKIGSSEQHNQRLKHLDYVRPDLSPQNESWVGVAD
                     LPAHLEQLRILVKEKTGRAMQQKATPIREGVIVINQGTSINQLRGLATAIELRWGIKTL
                     QIYTHKDEGHIDSDDTWKPNLHAHMVLDWVNHDTGKSIKLSKQDMAEMQTMVADCLQMV
                     RGESSDVKHLGAIQYKNQAEELRLQTLKEQTMKEEQARELALAEAKRMEQVASEQIIAE
                     NKKLGGLWMDWQKSFEASEKRRESITARKIEEDKLWEELGLKGAFKSFWEECKDWFRGF
                     GDGLKDLALLIWASPRGVMDKDVRCTADRKRGKLLLKWAYTRSR"
     gene            complement(2518..3495)
                     /gene="mob"
                     /label="mob"
     CDS             complement(4510..5043)
                     /codon_start=1
                     /product="hypothetical protein"
                     /label="hypothetical protein"
                     /note="orfE; hypothetical protein"
                     /protein_id="AVN57619.1"
                     /translation="MLVRGAEPMEKRQQRGLFTVPGLLLAFCSHGSSIADGLVAHPSEG
                     FYIIYTTSSEEFLTLSEEDYSLFASFANLVVREDSNSNGLRSAKPSGDGWKFGGKGKGK
                     LDAIHIGYELSKILGENQDFEIYVEYNGDGSYTVWYREVQKSSKGSNPVKTFNVLIRAN
                     EREGHTVSLFQPYA"
     rep_origin      complement(5013..5601)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5942..6676)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     CDS             complement(6912..7712)
                     /codon_start=1
                     /gene="ermF"
                     /product="ErmF"
                     /label="ermF"
                     /note="erythromycin resistance in Bacteroidetes"
                     /protein_id="AVN57621.1"
                     /translation="MTKKKLPVRFTGQHFTIDKVLIKDAIRQANISNQDTVLDIGAGKG
                     FLTVHLLKIANNVVAIENDTALVEHLRKLFSDARNVQVVGCDFRNFAVPKFPFKVVSNI
                     PYGITSDIFKILMFESLGNFLGGSIVLQLEPTQKLFSRKLYNPYTVFYHTFFDLKLVYE
                     VGPESFLPPPTVKSALLNIKRKHLFFDFKFKAKYLAFISCLLEKPDLSVKTALKSIFRK
                     SQVRSISEKFGLNLNAQIVCLSPSQWLNCFLEMLEVVPEKFHPS"
     gene            complement(6912..7712)
                     /gene="ermF"
                     /label="ermF"
     oriT            8269..8378
                     /label="oriT"
                     /note="incP origin of transfer"
     primer_bind     8847..8863
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    8864..8920
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8933..8949)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(8957..8973)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8981..9011)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9026..9047)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 gatccaattg agtgatttgt tctggttcag tgtcttggct tggctttaac aagtcaaggt
       61 tgttttggca cgaagttaac gccaaagaca atagcccgat tgctattgcg cagaatgttc
      121 tagttttcat agctgcttag gtttagtgtt ttgtattcaa gataagtctc tcttaagagg
      181 ttgtcttacc gcaaagataa agaattgcaa aaaatatatg caatacctac ttgttgcgat
      241 attctttgtt tcttccccaa aaaatcaacc ccaaaatgac cgtagaacga tgacgccaga
      301 aggaggtctc aaaaaacctc cttacctttg taccacaagt ggggatttta cttcattgat
      361 aatatacgcc cccccgtatg aatttttggg gagcgtagcg agccaaaaat tccacccaaa
      421 atgacgtaga aggaggtctc aaaaatcctc cttatctttg tgccacaagt ggcaaaaaaa
      481 aatatattga taatatatgc cccccgtatg attttttggg ggagcgaagc gacaccaaaa
      541 aatcacccca aaatgacgta gaaggagggt ctcaaaaatc ctccttatct ttgtgccaca
      601 agtggcaaaa aaaatatatt gataatatat gcccccccgt atgatttttt gggggagcga
      661 agcgacaccc aaaaatcacc ccaaaatgga cgttagaacg gagactgata attctaatat
      721 ttctcacgat ggtttttctc tctaaaaatt tatacttaga gaggtaatga gaacctttga
      781 atatcgatct cttaaaccct ttgagttctt ccaaataatc tttggttctt gtgaccataa
      841 tgacccgcaa tgccctatct atccttttga aaaagagctg tgccaagaat acatcgattg
      901 gagggctcaa caagcaggga gaaagtctaa gtccaaagca ggtaagaaag caatctataa
      961 tccagctcaa cctttccgct ataatgcttt ggtgcaagct gtctacagcg tagagcctga
     1021 tgcaggcagg gctaatgata atggcaggag gtctaagacg tgggtggtag tgggcgatgc
     1081 cacaagcgag gatgacatca aggtgtgtgg ttgggatagc ttagaggagc tgatggggcg
     1141 gtacaagttt gtggtaacaa ccccagccac ctacgtgggg cgcaggaggt tcaagagcaa
     1201 tgcccgctat ctgtatgccc tcgcctttga ccttgatgag gtcggagtgc cagagatgtc
     1261 agaggtaatc catcagcaga cgatagggat gaccccacaa gctaatatca tcgtcaactc
     1321 gggcgatggc atccacttgt actacatact tgctaagccg atgcccttat accccaaggt
     1381 gtacgagacg ctcaccaaca ttaaaaaagc tcttacacgg cttatatgga acaaagcgtc
     1441 ctctaggacg ggggaggaca accaagtgca aatacagccc attatacagc cgtttagggt
     1501 gccaggcagt cgtacgaagc acggggacat tgtaacagct tggcacaatg ctgatgcccc
     1561 actacacact attgaggagc tgaatcggtt tgctagcaag cctacactga agtaccttaa
     1621 ctcgggggtg tcgcaggagc aggcgcaagc gtttggacaa aggggcatac tcccacgaat
     1681 atggctaaca aaagcaagag ctaaagagct atacccagat tggtatcaaa aacgcatcgt
     1741 agagggcgca cctcgtaaga cgtggcatgt gcataggggg ctgtatgagt ggtggctaca
     1801 tatgctgtgg acgaacgaaa aggtcgctgt tgggcatcgc taccactgca ttatgttctt
     1861 agcagtatat gccaagaagt gtaacgtgcc atttgacata ctcaaacggg acgctctaga
     1921 gcttgtgcca cgaatggaag cactaacggg caacacgggc aggcacttct ccgtgcagga
     1981 cgctctagac gctctaaaag catataagga gagctcagag acataccctc gccagctgat
     2041 agaggatagg tcggggctaa gaatagaacc taacaaacgc aatgggagac cgcaagactt
     2101 acatctaaag ctcgcaagag gctctagaga tattttgcag gaagcaaagg gaagaaaaga
     2161 ttggagagag ggtaacggaa gaccaaaagg ctctatcgta ctagcggaag acagcccgca
     2221 atacgccaag gtgcaggagt ggagggatag aaacccaaac agcaacaaca agagccgttg
     2281 tgcgagagag acaggactaa gtagaccgac ccgtccgcaa gtggtggcaa agcccttggg
     2341 aaacgcttta agaacatagt gcggtcaaat cttgcgaccc ctgcgctctc gtctctcttg
     2401 gtcaacacgc ttctctctgc gtctgtcttg tatctctagc ctgcctcgta gcgcactcac
     2461 tgacggattg cgcctgcctt tgtatggggg agcttggtac aactatcttg ggtgctctta
     2521 cctgcttcta gtgtatgccc atttgagcag taatttcccc ctcttccgat ccgctgtgca
     2581 cctcacgtct ttgtccatca ctcctctagg cgaagcccat atcagcagag ctaagtcttt
     2641 gagcccatcg ccgaaacctc taaaccaatc cttgcattcc tcccaaaacg atttaaaggc
     2701 tcctttcaac ccaagttctt cccatagctt atcctcctct atcttacgag ccgttatgct
     2761 ctctcggcgc ttctcagagg cctcaaatga tttttgccag tccatccaca gccctccaag
     2821 cttcttattc tctgcgataa tctgctcaga cgcaacctgc tccatccgct tagcttcggc
     2881 tagtgccagc tcccgagcct gttcttcttt catcgtttgt tctttaaggg tttggagacg
     2941 cagctcttcg gcttgattct tgtattgtat agccccgagg tgtttgacat cgctactctc
     3001 tcccctaacc atttgcaggc agtccgccac catcgtctgc atctccgcca tatcttgctt
     3061 gctaagcttg atactcttgc ccgtgtcgtg gttcacccaa tcaagcacca tatgggcatg
     3121 taggtttggc ttccacgtgt catcgctatc tatatgcccc tcgtccttgt gggtgtatat
     3181 ctgtagcgtc ttaatacccc atcgcagttc tattgccgtt gcgagacccc ttaattggtt
     3241 aatcgaggtg ccttgattga ttactattac accctctctt atcggggttg ctttctgttg
     3301 catcgctctg cccgtcttct cctttacaag tattcggagt tgttcaagat gcgcgggcaa
     3361 atctgctacg ccaacccaac tctcgttttg tggggagagg tcgggtcgca cataatcaag
     3421 atgcttcaag cgttgattat gttgctcact actgccgatt ttacacggct cgatatggat
     3481 acttgttttg ctcatcgctt gtacttttgg gtcaacttta gggggtgctg ggggtgtcct
     3541 ccatgcccct cggagagcac acaactacgg tatgtagtta taatgtgcta taactatggt
     3601 cgtagctagc gcctgcccaa agttacggag aacgatttta ttggacaact tcttgtcgct
     3661 cccattacac acacatttgg tgcgtgaact gacgcccagc cgacagacgt ttatttggga
     3721 gatttgtcta tatttgcagt gaatataatc aagtagaata taatagaaat ggataatatt
     3781 gagctaaaag catccacgtc aattagaaat tgatgctagt gtactccagc agatacgcag
     3841 tgaagcaagg gctgagaata gaagcctaag caactatatt gagaccttac tctaccgaat
     3901 ggggtataga ccttataatg ccgagacggc agaggcctgc atagacgcta agagcggtcg
     3961 ctatgccggc tctgtggata caactacccg agaaacaata gaagcgtctt tgttttggaa
     4021 atgaggaagc gtaggctggt atatactacc aagtttaaga aagaccttaa gcgatttaga
     4081 aacgacctca aaaggcgctc taaaatcgta gatgtcattg aaatgctgtc taagggtgtg
     4141 gagataccct tagaaatgcg tcctcacaag cttataggga actatgaggg ctgtatggag
     4201 ctacacattg agggtgacct gctcttgata tggatagagc agataagcgc agacgaggag
     4261 cttatcgtcc tctcacgctt aggctcgcac gcagatctct tttagtaact ttggcaagtg
     4321 agatagagct actaagtagc gccacggtga tcctctgaca tcgttatggt tcgtggcttt
     4381 gggcttagta gctctatttc tgttatgtca tatccatggt atttgcctat catattttca
     4441 aaacgaaaaa cagaaataga gctgacctat caagccagct ctatttaatc acatcgagta
     4501 agcaatcgtt caagcatacg gttgaaagag agagacggta tgcccctctc tttcgttggc
     4561 tcttatgagg acattaaaag tctttacggg attactccct ttagacgatt tttggacttc
     4621 cctataccaa acggtataag acccatcgcc attgtattct acatagatct caaaatcttg
     4681 attttctcct aggattttcg ataattcata gccgatgtgg atggcatcta gtttcccctt
     4741 tcctttacct ccaaatttcc agccatcccc agagggtttt gcgcttctta agccatttga
     4801 attggagtcc tctcgaacaa ccaaattggc aaaagaagcg aacagagaat aatcttcttc
     4861 agataacgtg agaaactcct ctgaagaggt ggtatagatg atatagaacc cttcagaggg
     4921 gtgtgcaaca agaccatctg caatagagga tccatgtgag caaaaggcca gcaaaaggcc
     4981 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5041 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5101 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5161 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5221 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5281 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5341 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5401 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     5461 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5521 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5581 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5641 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5701 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5761 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     5821 cgttcatcca tagttgcctg actcgatccc cgaagctgtc agtagtatac ctaataattt
     5881 atctacattc cctttagtaa cgtgtaactt tccaaattta caaaagcgac tcatagaatt
     5941 atttcctccc gttaaataat agataactat taaaaataga caatacttgc tcataagtaa
     6001 cggtacttaa attgtttact ttggcgtgtt tcattgcttg atgaaactga tttttagtaa
     6061 acagttgacg atattctcga ttgacccatt ttgaaacaaa gtacgtatat agcttccaat
     6121 atttatctgg aacatctgtg gtatggcggg taagttttat taagacactg tttacttttg
     6181 gtttaggatg aaagcattcc gctggcagct taagcaattg ctgaatcgag acttgagtgt
     6241 gcaagagcaa ccctagtgtt cggtgaatat ccaaggtacg cttgtagaat ccttcttcaa
     6301 caatcagata gatgtcagac gcatggcttt caaaaaccac ttttttaata atttgtgtgc
     6361 ttaaatggta aggaatactc ccaacaattt tatacctctg tttgttaggg aattgaaact
     6421 gtagaatatc ttggtgaatt aaagtgacac gagtattcag ttttaatttt tctgacgata
     6481 agttgaatag atgactgtct aattcaatag acgttacctg tttacttatt ttagccagtt
     6541 tcgtcgttaa atgcccttta cctgttccaa tttcgtaaac ggtatcggtt tcttttaaat
     6601 tcaattgttt tattatttgg ttgagtactt tttcactcgt taaaaagttt tgagaatatt
     6661 ttatattttt gttcatgtaa tcactccttc ttaattacaa atttttagca tctaatttaa
     6721 cttcaattcc tattatacaa aattttaaga tactgcacta tcaacacact cttaagtttg
     6781 cttctaagtc ttatttccat aacttctttt acgtttccgc tccatcgcca atttgccagc
     6841 cgttatgcgg cagcttaaaa aaagacgaca ccaaaccaaa aaaatcatct tgacaaccac
     6901 ccgactttga actacgaagg atgaaatttt tcagggacaa cttccagcat ttccaaaaaa
     6961 cagtttaacc attgacttgg agacaaacaa acaatttgag cattaaggtt taaaccgaat
     7021 ttttccgaaa ttgacctgac ctgacttttc ctgaaaatcg actttaaagc tgtttttaca
     7081 gataaatcag gtttctctaa cagacaggaa ataaatgcta agtatttggc tttaaactta
     7141 aaatcaaaaa ataagtgttt tcttttaatg tttaacaggg ctgatttgac agttggcggt
     7201 ggcaagaaac tttcaggacc tacctcatag acaagtttca aatcaaaaaa agtatgatag
     7261 aaaacggtat atggattgta aagcttcctc gaaaataact tttgtgtagg ttctaattga
     7321 aggacaatgg aacctcccag aaaatttcca agactctcaa acatcaggat tttgaaaata
     7381 tcggaagtaa tgccataagg aatatttgac accactttga aaggaaattt cggaactgca
     7441 aaattcctaa aatcacaacc gacaacttga acatttcggg catcagaaaa taattttcgt
     7501 aaatgttcaa ccaaagctgt gtcgttttca atagcaacaa cattgttggc gatttttaat
     7561 aaatgaacag taagaaaccc cttgcctgcc ccaatatcta aaaccgtatc ctgattactt
     7621 atatttgctt gtcttattgc atcttttatt agcactttat caatagtaaa gtgctgaccc
     7681 gtaaaacgaa cgggcaattt cttttttgtc attagtaact tcttacaggt gaatacttct
     7741 tgagttcaac ttataaatgc aactttttgg gtgcggataa taagcaataa aaacatttat
     7801 ttttcagaga ggaaagagag acaatgtccc cctttctctc actctgaatg gataaagttt
     7861 gctatctttg ctttaattgt ccgtccaaag aaaaaaaagt tgcagatgag caaacatata
     7921 accgaggaac aaaggtatgc aatttctatg atgttgcaaa taccgatgag caaaaaagca
     7981 atagcggaag ctatcggggg taccgagctc gaattattat tgaagcattt atcagggtta
     8041 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8101 gcgcacattt ccccgaaaag tgccacctga cgtcttttcc gctgcataac cctgcttcgg
     8161 ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg
     8221 ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg
     8281 tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc
     8341 ggtgctcaac gggaatcctg ctctgcgagg ctggccggct accgccggcg taacagatga
     8401 gggcaagcgg atggctgatg aaaccaagcc aaccaggaag ggcagcccac ctatcaaggt
     8461 gtacgtctta gctctgctga tatgggcttc gcctagagga gtgatggact cccggagacg
     8521 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     8581 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     8641 gtgcaccatc ntgatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     8701 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     8761 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     8821 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattcg agctcggtac
     8881 ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc
     8941 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     9001 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     9061 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     9121 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     9181 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9241 tatccacaga atcaggggat aacgcaggaa agaacatg
//
