LOCUS       Exported                8739 bp DNA     circular SYN 10-OCT-2024
DEFINITION  Exported.
ACCESSION   V012822
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8739)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8739)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8739)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3573..3958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          4763..4822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             98..109
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             206..259
                     /label=S loop
                     /note="GroES chaperone mobile loop that interacts with
                     GroEL"
     CDS             498..2141
                     /gene="groEL"
                     /label=Chaperonin GroEL
                     /note="Chaperonin GroEL from Escherichia coli O8 (strain
                     IAI1). Accession#: B7M8Q4"
     CDS             2202..3497
                     /label=Trigger Factor
                     /note="ribosome-associated chaperone from E. coli (Hoffmann
                     et al., 2010)"
     terminator      4833..4919
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        complement(4979..5034)
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             5050..5673
                     /label=TetR
                     /note="tetracycline repressor TetR"
     CDS             complement(6696..7352)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(7353..7455)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(7981..8526)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt ccctatcagt gatagagtat aatagagtcg
       61 aattgttagc ggagaagaat ttcacacaga attcattaaa gaggagaaag tcgaggcgtc
      121 acccataaca gatacggact ttctcaaagg agagttatca atgaatattc gtccattgca
      181 tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa tctgctggcg gcatcgttct
      241 gaccggctct gcagcggcta aatccacccg cggcgaagtg ctggctgtcg gcaatggccg
      301 tatccttgaa aatggcgaag tgaagccgct ggatgtgaaa gttggcgaca tcgttatttt
      361 caacgatggc tacggtgtga aatctgagaa gatcgacaat gaagaagtgt tgatcatgtc
      421 cgaaagcgac attctggcaa ttgttgaagc gtaatccgcg cacgacactg aacatacgaa
      481 tttaaggaat aaagataatg gcagctaaag acgtaaaatt cggtaacgac gctcgtgtga
      541 aaatgctgcg cggcgtaaac gtactggcag atgcagtgaa agttaccctc ggtccaaaag
      601 gccgtaacgt agttctggat aaatctttcg gtgcaccgac catcaccaaa gatggtgttt
      661 ccgttgctcg tgaaatcgaa ctggaagaca agttcgaaaa tatgggtgcg cagatggtga
      721 aagaagttgc ctctaaagca aacgacgctg caggcgacgg taccaccact gcaaccgtac
      781 tggctcaggc tatcatcact gaaggtctga aagctgttgc tgcgggcatg aacccgatgg
      841 acctgaaacg tggtatcgac aaagcggtta ccgctgcagt tgaagaactg aaagcgctgt
      901 ccgtaccatg ctctgactct aaagcgattg ctcaggttgg taccatctcc gctaactccg
      961 acgaaaccgt aggtaaactg atcgctgaag cgatggacaa agtcggtaaa gaaggcgtta
     1021 tcaccgttga agacggtacc ggtctgcagg acgaactgga cgtggttgaa ggtatgcagt
     1081 tcgaccgtgg ctacctgtct ccttacttca tcaacaagcc ggaaactggc gcagtagaac
     1141 tggaaagccc gttcatcctg ctggctgaca agaaaatctc caacatccgc gaaatgctgc
     1201 cggttctgga agctgttgcc aaagcaggca aaccgctgct gatcatcgct gaagatgtag
     1261 aaggcgaagc gctggcaact ctggttgtta acaccatgcg tggcatcgtg aaagtcgctg
     1321 cggttaaagc accgggcttc ggcgatcgtc gtaaagctat gctgcaggat atcgcaaccc
     1381 tgactggcgg taccgtgatc tctgaagaga tcggtatgga gctggaaaaa gcaaccctgg
     1441 aagacctggg tcaggctaaa cgtgttgtga tcaacaaaga caccaccact atcatcgatg
     1501 gcgtgggtga agaagctgca atccagggcc gtgttgctca gatccgtcag cagattgaag
     1561 aagcaacttc tgactacgac cgtgaaaaac tgcaggaacg cgtagcgaaa ctggcaggcg
     1621 gcgttgcagt tatcaaagtg ggtgctgcta ccgaagttga aatgaaagag aaaaaagcac
     1681 gcgttgaaga tgccctgcac gcgacccgtg ctgcggtaga agaaggcgtg gttgctggtg
     1741 gtggtgttgc gctgatccgc gtagcgtcta aactggctga cctgcgtggt cagaacgaag
     1801 accagaacgt gggtatcaaa gttgcactgc gtgcaatgga agctccgctg cgtcagatcg
     1861 tattgaactg cggcgaagaa ccgtctgttg ttgctaacac cgttaaaggc ggcgacggca
     1921 actacggtta caacgcagca accgaagaat acggcaacat gatcgacatg ggtatcctgg
     1981 atccaaccaa agtaactcgt tctgctctgc agtacgcagc ttctgtggct ggcctgatga
     2041 tcaccaccga atgcatggtt accgacctgc cgaaaaacga tgcagctgac ttaggcgctg
     2101 ctggcggtat gggcggcatg ggtggcatgg gcggcatgat gtaattgccc tgcacctcgc
     2161 agaaataaac aaacccccct gtgatttttt gaggtaacaa gatgcaagtt tcagttgaaa
     2221 ccactcaagg ccttggccgc cgtgtaacga ttactatcgc tgctgacagc atcgagaccg
     2281 ctgttaaaag cgagctggtc aacgttgcga aaaaagtacg tattgacggc ttccgcaaag
     2341 gcaaagtgcc aatgaatatc gttgctcagc gttatggcgc gtctgtacgc caggacgttc
     2401 tgggtgacct gatgagccgt aacttcattg acgccatcat taaagaaaaa atcaatccgg
     2461 ctggcgcacc gacttatgtt ccgggcgaat acaagctggg tgaagacttc acttactctg
     2521 tagagtttga agtttatccg gaagttgaac tgcagggtct ggaagcgatc gaagttgaaa
     2581 aaccgatcgt tgaagtgacc gacgctgacg ttgacggcat gctggatact ctgcgtaaac
     2641 agcaggcgac ctggaaagaa aaagacggcg ctgttgaagc agaagaccgc gtaaccatcg
     2701 acttcaccgg ttctgtagac ggcgaagagt tcgaaggcgg taaagcgtct gatttcgtac
     2761 tggcgatggg ccagggtcgt atgatcccgg gctttgaaga cggtatcaaa ggccacaaag
     2821 ctggcgaaga gttcaccatc gacgtgacct tcccggaaga ataccacgca gaaaacctga
     2881 aaggtaaagc agcgaaattc gctatcaacc tgaagaaagt tgaagagcgt gaactgccgg
     2941 aactgactgc agaattcatc aaacgtttcg gcgttgaaga tggttccgta gaaggtctgc
     3001 gcgctgaagt gcgtaaaaac atggagcgcg agctgaagag cgccatccgt aaccgcgtta
     3061 agtctcaggc gatcgaaggt ctggtaaaag ctaacgacat cgacgtaccg gctgcgctga
     3121 tcgacagcga aatcgacgtt ctgcgtcgcc aggctgcaca gcgtttcggt ggcaacgaaa
     3181 aacaagctct ggaactgccg cgcgaactgt tcgaagaaca ggctaaacgc cgcgtagttg
     3241 ttggcctgct gctgggcgaa gttatccgca ccaacgagct gaaagctgac gaagagcgcg
     3301 tgaaaggcct gatcgaagag atggcttctg cgtacgaaga tccgaaagaa gttatcgagt
     3361 tctacagcaa aaacaaagaa ctgatggaca acatgcgcaa tgttgctctg gaagaacagg
     3421 ctgttgaagc tgtactggcg aaagcgaaag tgactgaaaa agaaaccact ttcaacgagc
     3481 tgatgaacca gcaggcgtaa tttacgcagc ataacgcgct aaattcgcac aaaggcccgt
     3541 caccgccagg tggtgggctt ttttttgtca tggcgtgaag atggccgacg gtgcggcgtc
     3601 ggtggaagtg ggtgcgttga ccctgaccga cttcgtcaat ccggccggcc tgcaggcccg
     3661 cggcgagaac ctgttcctgg agaccaccgc gtccggcccg gcacagaacg gcaatcccgg
     3721 cctcaatggc ctgggcaccg tggtgcaggg tgcgctggaa ggcagcaacg tcaatgtggt
     3781 ggaagagctg gtgtcgatga tcgaaaccca gcgcgcctac gaaatgaacg ccaaggcgat
     3841 ctccaccacc gattcgatgc tcggctatct caacaacaag ctctgaccgg ccaaaccggg
     3901 aaaccgccat gtcgcccatt tccaacttca cccgcactgc cctcgcctgc gccgtggcta
     3961 aggaacgcgt catttttctg actggccagg ttgaagacca catggctaac ctgattgtgg
     4021 cgcagatgct gttcctggaa gcggaaaacc cagaaaaaga tatctatctg tacattaact
     4081 ccccaggcgg ggtgatcact gccgggatgt ctatctatga caccatgcag tttatcaagc
     4141 ctgatgtcag caccatctgt atgggccagg cggcctcgat gggcgctttc ttgctgaccg
     4201 caggggcaaa aggtaaacgt ttttgcctgc cgaattcgcg cgtgatgatt caccaaccgt
     4261 tgggcggcta ccagggccag gcgaccgata tcgaaattca tgcccgtgaa attctgaaag
     4321 ttaaagggcg catgaatgaa cttatggcgc ttcatacggg tcaatcatta gaacagattg
     4381 aacgtgatac cgagcgcgat cgcttccttt ccgcccctga agcggtggaa tacggtctgg
     4441 tcgattcgat tctgacccat cgtaattgat gccagaggcg caactgtgcc gctatactta
     4501 tccagggcgg cacaacgctg taagcggctt gcgcctgaga atggcatttg cgtcgtcgtg
     4561 tgcggcacaa agaacaaaga agaggttttg acccatgaca gataaacgca aagatggctc
     4621 aggcaaattg ctgtattgct ctttttgcgg caaaagccag catgaagtgc gcaagctgat
     4681 tgccggtcca tccgtgtata tctgcgacga atgtgttgat ttatgtaacg acatcattcg
     4741 gggcagaaat gtctgggggt ttttcttttg gtcatctttc tagtataaga ttcagacacg
     4801 gacgacgcga gtggcgcctc gactagaggc atcaaataaa acgaaaggct cagtcgaaag
     4861 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     4921 cgccgcccta gacctaggtc gactctagag gatctactag tcatatggat tttcactttt
     4981 ctctatcact gatagggagt ggtaaaataa ctctatcaac gatagagtgt caacaaaaat
     5041 taggaattaa tgatgtctag attagataaa agtaaagtga ttaacagcgc attagagctg
     5101 cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta
     5161 gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc
     5221 attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa
     5281 gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga
     5341 gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa
     5401 ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct
     5461 gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa
     5521 gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta
     5581 tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga
     5641 ttagaaaaac aacttaaatg tgaaagtggg tcttaaaagc agcataacct ttttccgtga
     5701 tggtaacttc acggtaacca agatgtcgag ttaaccaccc tttagattca taaagcgaaa
     5761 ataatgcggc tccaacgtac ccacctaaat ggaaacggaa tcggatcctc gggcagcgtt
     5821 gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg
     5881 cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac
     5941 tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt
     6001 tcgtaaagtc tggaaacgcg gaagtcccct acgtgctgct gaagttgccc gcaacagaga
     6061 gtggaaccaa ccggtgatac cacgatacta tgactgagag tcaacgccat gagcggcctc
     6121 atttcttatt ctgagttaca acagtccgca ccgctgccgg tagctccttc cggtgggcgc
     6181 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     6241 aggtgccggc agcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     6301 aacaagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct
     6361 gtggaacacc tacatctgta ttaacgaagc gctaaccgtt tttatcaggc tctgggaggc
     6421 agaataaatg atcatatcgt caattattac ctccacgggg agagcctgag caaactggcc
     6481 tcaggcattt gagaagcaca cggtcacact gcttccggta gtcaataaac cggtaaacca
     6541 gcaatagaca taagcggcta tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt
     6601 gctttcgaat ttctgccatt catccgctta ttatcactta ttcaggcgta gcaaccaggc
     6661 gtttaagggc accaataact gccttaaaaa aattacgccc cgccctgcca ctcatcgcag
     6721 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     6781 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     6841 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     6901 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc
     6961 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     7021 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     7081 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc
     7141 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     7201 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     7261 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     7321 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
     7381 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     7441 cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca
     7501 acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg
     7561 gcgcaaagtg cgtcgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg
     7621 aggtgctcca gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt
     7681 ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctag cggagtgtat actggcttac
     7741 tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg
     7801 caccggtgcg tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact
     7861 cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt
     7921 tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt
     7981 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg
     8041 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg
     8101 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc
     8161 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
     8221 acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8281 acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag
     8341 ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc
     8401 gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa
     8461 ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga
     8521 tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc
     8581 tcttcaaatg tagcacctga agtcagcccc atacgatata agttgtaatt ctcatgtttg
     8641 acagcttatc atcgataagc ttgcatgccc taagaaacca ttattatcat gacattaacc
     8701 tataaaaata ggcgtatcac gaggcccttt cgtcttcac
//
