LOCUS       Exported               11193 bp DNA     circular SYN 28-NOV-2025
DEFINITION  Exported.
ACCESSION   V012941
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11193)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 11193)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1787..3700
                     /gene="dnaK"
                     /label=Chaperone protein DnaK
                     /note="Chaperone protein DnaK from Escherichia coli O1:K1 /
                     APEC. Accession#: A1A766"
     CDS             3792..4919
                     /gene="dnaJ"
                     /label=Chaperone protein DnaJ
                     /note="Chaperone protein DnaJ from Escherichia coli (strain
                     K12). Accession#: P08622"
     CDS             4939..5529
                     /gene="grpE"
                     /label=Protein GrpE
                     /note="Protein GrpE from Escherichia coli (strain K12). 
                     Accession#: P09372"
     terminator      5835..5921
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      6013..6040
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        6060..6150
                     /label=AmpR promoter
     rep_origin      6476..7021
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(7375..7998)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     promoter        8014..8069
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             8385..8438
                     /label=S loop
                     /note="GroES chaperone mobile loop that interacts with
                     GroEL"
     CDS             8677..10320
                     /gene="groEL"
                     /label=Chaperonin GroEL
                     /note="Chaperonin GroEL from Escherichia coli O8 (strain
                     IAI1). Accession#: B7M8Q4"
     terminator      10450..10536
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        10872..10974
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             join(10975..11193,1..438)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
       61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
      181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
      241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
      301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
      361 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
      421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
      481 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgaa tagttacggc
      541 ttatgacatc tttgtggaca catcattcac tttttattca catccggccc tgaactcgct
      601 aggacttgcc ccggtgcatt ttttaaatac ccgcgaaaaa tagagctgat cgtcaaatcc
      661 aacattgcgc ccaacggtcg ctatcggcat tcgcgtagtg ctaagcagaa gtttcgcctg
      721 gctgatacgc tgatcttcgc gccagctcaa tacgctaatg cctaactgct ggcggaacag
      781 atgtgataac cgggagggcg acaggcagac atgctgggcg acgctggcga tatcaaaatg
      841 gctgtccgcc agatggtcgc tgatatactg gcaggcatcg cgcacacggc tatccatcgg
      901 cgggtgcaac gactcattaa ttaccgccat acgtctgagc aacaactgct ccagcagatt
      961 gatcgccagt agctcagaat agcgaccttc cccttgcccg gcgctgatga tctgcccgaa
     1021 cagttcgctg aaatgcggct ggcgcgcctc gtccgggcgg aaaaatcctg tctgggcaaa
     1081 gattgtcggc caggtcagcc actcctgcca gtaggcgcga ggccggaaat aaacccactg
     1141 gtgataccac tcgctggcgt ccggatgccg tccatagtga tgaatctcgc ccggcggaaa
     1201 caataatata tcgccaggcc gacagacaaa ctgctcgcca ttattattaa tgacgccctc
     1261 tccgcggatg gtcaggttaa gaatatatcc cttcatgccc aacggacgat cgataaaaaa
     1321 atccagatat ccattcgctt caattggcgt cagcccggcg accagatggg cattaaatga
     1381 atatcccggc aatagcggat cattttgcgt ttcagccatg atttctctac cccccgatgt
     1441 tcagagaaga aacaaattgt ccatatcgac caggacgaca gagcttccgt ctccgcaaga
     1501 ctttgcgctt gatgaaagca cgtatcaacc ccgcttgtga aaagcgcttt gtaacaaaag
     1561 cgtacagttc aggcgataaa attaagtaac agaagtgtct ataactatgg ctggaatgtc
     1621 cacattgaat atttgcacag cgtcacactt tgcaaagcat tagcattttt gtccataaga
     1681 ttagcggatc ctgcctgacg gtttttgccg cgactctcta taatttctcc atacctgttt
     1741 ttctggatgg agtaagacca tggctatgga ttgtggagac gtttagatgg gtaaaataat
     1801 tggtatcgac ctgggtacta ccaactcttg tgtagcgatt atggatggca ccactcctcg
     1861 cgtgctggag aacgccgaag gcgatcgcac cacgccttct atcattgcct atacccagga
     1921 tggtgaaact ctagttggtc agccggctaa acgtcaggca gtgacgaacc cgcaaaacac
     1981 tctgtttgcg attaaacgcc tgattggtcg ccgcttccag gacgaagaag tacagcgtga
     2041 tgtttccatc atgccgttca aaattattgc tgctgataac ggcgacgcat gggtcgaagt
     2101 taaaggccag aaaatggcac cgccgcagat ttctgctgaa gtgctgaaaa aaatgaagaa
     2161 aaccgctgaa gattacctgg gtgaaccggt aactgaagct gttatcaccg taccggcata
     2221 ctttaacgat gctcagcgtc aggcaaccaa agacgcaggc cgtatcgctg gtctggaagt
     2281 aaaacgtatc atcaacgaac cgaccgcagc tgcgctggct tacggtctgg acaaaggcac
     2341 tggcaaccgt actatcgcgg tttatgacct gggtggtggt actttcgata tttctattat
     2401 cgaaatcgac gaagttgacg gcgaaaaaac cttcgaagtt ctggcaacca acggtgatac
     2461 ccacctgggg ggtgaagact tcgacagccg tctgatcaac tatctggttg aagaattcaa
     2521 gaaagatcag ggcattgacc tgcgcaacga tccgctggca atgcagcgcc tgaaagaagc
     2581 ggcagaaaaa gcgaaaatcg aactgtcttc cgctcagcag accgacgtta acctgccata
     2641 catcactgca gacgcgaccg gtccgaaaca catgaacatc aaagtgactc gtgcgaaact
     2701 ggaaagcctg gttgaagatc tggtaaaccg ttccattgag ccgctgaaag ttgcactgca
     2761 ggacgctggc ctgtccgtat ctgatatcga cgacgttatc ctcgttggtg gtcagactcg
     2821 tatgccaatg gttcagaaga aagttgctga gttctttggt aaagagccgc gtaaagacgt
     2881 taacccggac gaagctgtag caatcggtgc tgctgttcag ggtggtgttc tgactggtga
     2941 cgtaaaagac gtactgctgc tggacgttac cccgctgtct ctgggtatcg aaaccatggg
     3001 cggtgtgatg acgacgctga tcgcgaaaaa caccactatc ccgaccaagc acagccaggt
     3061 gttctctacc gctgaagaca accagtctgc ggtaaccatc catgtgctgc agggtgaacg
     3121 taaacgtgcg gctgataaca aatctctggg tcagttcaac ctagatggta tcaacccggc
     3181 accgcgcggc atgccgcaga tcgaagttac cttcgatatc gatgctgacg gtatcctgca
     3241 cgtttccgcg aaagataaaa acagcggtaa agagcagaag atcaccatca aggcttcttc
     3301 tggtctgaac gaagatgaaa tccagaaaat ggtacgcgac gcagaagcta acgccgaagc
     3361 tgaccgtaag tttgaagagc tggtacagac tcgcaaccag ggcgaccatc tgctgcacag
     3421 cacccgtaag caggttgaag aagcaggcga caaactgccg gctgacgaca aaactgctat
     3481 cgagtctgcg ctgactgcac tggaaactgc tctgaaaggt gaagacaaag ccgctatcga
     3541 agcgaaaatg caggaactgg cacaggtttc ccagaaactg atggaaatcg cccagcagca
     3601 acatgcccag cagcagactg ccggtgctga tgcttctgca aacaacgcga aagatgacga
     3661 tgttgtcgac gctgaatttg aagaagtcaa agacaaaaaa taatcgccct ataaacgggt
     3721 aattatactg acacgggcga aggggaattt cctccccgcc cgtgcattca tctaggggca
     3781 atttaaaaaa gatggctaag caagattatt acgagatttt aggcgtttcc aaaacagcgg
     3841 aagagcgtga aatcagaaag gcctacaaac gcctggccat gaaataccac ccggaccgta
     3901 accagggtga caaagaggcc gaggcgaaat ttaaagagat caaggaagct tatgaagttc
     3961 tgaccgactc gcaaaaacgt gcggcatacg atcagtatgg tcatgctgcg tttgagcaag
     4021 gtggcatggg cggcggcggt tctggcggcg gcgcagactt cagcgatatt tttggtgacg
     4081 ttttcggcga tatttttggc ggcggacgtg gtcgtcaacg tgcggcgcgc ggtgctgatt
     4141 tacgctataa catggagctc accctcgaag aagctgtacg tggcgtgacc aaagagatcc
     4201 gcattccgac tctggaagag tgtgacgttt gccacggtag cggtgcaaaa ccaggtacac
     4261 agccgcagac ttgtccgacc tgtcatggtt ctggtcaggt gcagatgcgc cagggattct
     4321 tcgctgtaca gcagacctgt ccacactgtc agggccgcgg tacgctgatc aaagatccgt
     4381 gcaacaaatg tcatggtcat ggtcgtgttg agcgcagcaa aacgctgtcc gttaaaatcc
     4441 cggcaggggt ggacactgga gaccgcatcc gtcttgcggg cgaaggtgaa gcgggcgagc
     4501 atggcgcacc ggcaggcgat ctgtacgttc aggttcaggt taaacagcac ccgattttcg
     4561 agcgtgaagg caacaacctg tattgcgaag tcccgatcaa cttcgctatg gcggcgctgg
     4621 gtggcgaaat cgaagtaccg acccttgatg gtcgcgtcaa actgaaagtg cctggcgaaa
     4681 cccagaccgg taagctattc cgtatgcgcg gtaaaggcgt caagtctgtc cgcggtggcg
     4741 cgcagggtga tttgctgtgc cgcgttgtcg tcgaaacacc ggtaggcctg aacgaaaggc
     4801 agaaacagct gctgcaagag ctgcaagaaa gcttcggtgg cccaaccggc gagcacaaca
     4861 gcccgcgctc aaagagcttc tttgatggtg tgaagaagtt ttttgacgac ctgacccgct
     4921 aacctcgcgg agaaattcat gagtagtaaa gaacagaaaa cgcctgaggg gcaagccccg
     4981 gaagaaatta tcatggatca gcacgaagag attgaggcag ttgagccaga agcttctgct
     5041 gagcaggtgg atccgcgcga tgaaaaagtt gcgaatctcg aagctcagct ggctgaagcc
     5101 cagacccgtg aacgtgacgg cattttgcgt gtaaaagccg aaatggaaaa cctgcgtcgt
     5161 cgtactgaac tggatattga aaaagcccac aaattcgcgc tggagaaatt catcaacgaa
     5221 ttgctgccgg tgattgatag cctggatcgt gcgctggaag tggctgataa agctaacccg
     5281 gatatgtctg cgatggttga aggcattgag ctgacgctga agtcgatgct ggatgttgtg
     5341 cgtaagtttg gcgttgaagt gatcgccgaa actaacgtcc cactggaccc gaatgtgcat
     5401 caggccatcg caatggtgga atctgatgac gttgcgccag gtaacgtact gggcattatg
     5461 cagaagggtt atacgctgaa tggtcgtacg attcgtgcgg cgatggttac tgtagcgaaa
     5521 gcaaaagctt aatttctgct ttcgtaataa ttcacggccc tgcatgcaag ctcagatctg
     5581 agcttggctg ttttggcgga tgagagaaga ggtcgactct agaggatcta ctagtcatag
     5641 cgccgatatc agatctgttt tcagcctgat acagattaaa tcagaacgca gaagcggtct
     5701 gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa
     5761 ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg
     5821 gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     5881 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
     5941 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc
     6001 atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt
     6061 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6121 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6181 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6241 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6301 agcggtaaga tccttgagag ttttcgcccc gaagaacgca agcttatcga tgataagctg
     6361 tcaaacatga gaattacaac ttatatcgta tggggctgac ttcaggtgct acatttgaag
     6421 agataaattg cactgaaatc tagaaatatt ttatctgatt aataagatga tcttcttgag
     6481 atcgttttgg tctgcgcgta atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg
     6541 gtttttcgaa ggttctctga gctaccaact ctttgaaccg aggtaactgg cttggaggag
     6601 cgcagtcacc aaaacttgtc ctttcagttt agccttaacc ggcgcatgac ttcaagacta
     6661 actcctctaa atcaattacc agtggctgct gccagtggtg cttttgcatg tctttccggg
     6721 ttggactcaa gacgatagtt accggataag gcgcagcggt cggactgaac ggggggttcg
     6781 tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg
     6841 agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
     6901 gcgcacgagg gagccgccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6961 ccaccactga tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa
     7021 aaacggcttt gccgcggccc tctcacttcc ctgttaagta tcttcctggc atcttccagg
     7081 aaatctccgc cccgttcgta agccatttcc gctcgccgca gtcgaacgac cgagcgtagc
     7141 gagtcagtga gcgaggaagc ggaatatatc ctgtatcaca tattctgctg acgcaccggt
     7201 gcagcctttt ttctcctgcc acatgaagcc ggtacccggg gatccgattc cgtttccatt
     7261 taggtgggta cgttggagcc gcattatttt cgctttatga atctaaaggg tggttaactc
     7321 gacatcttgg ttaccgtgaa gttaccatca cggaaaaagg ttatgctgct tttaagaccc
     7381 actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa
     7441 ggctggctct gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat
     7501 actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa
     7561 tacgcaacct aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga
     7621 aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt
     7681 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa
     7741 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg
     7801 agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt
     7861 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt
     7921 taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt
     7981 atctaatcta gacatcatta attcctaatt tttgttgaca ctctatcgtt gatagagtta
     8041 ttttaccact ccctatcagt gatagagaaa agtgaaaatc catatgacta gtagatcctc
     8101 tagagtcgac taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     8161 gaggcccttt cgtcttcacc tcgagaaatc ataaaaaatt tatttgcttc cctatcagtg
     8221 atagagtata atagagtcga attgttagcg gagaagaatt tcacacagaa ttcattaaag
     8281 aggagaaagt cgaggcgtca cccataacag atacggactt tctcaaagga gagttatcaa
     8341 tgaatattcg tccattgcat gatcgcgtga tcgtcaagcg taaagaagtt gaaactaaat
     8401 ctgctggcgg catcgttctg accggctctg cagcggctaa atccacccgc ggcgaagtgc
     8461 tggctgtcgg caatggccgt atccttgaaa atggcgaagt gaagccgctg gatgtgaaag
     8521 ttggcgacat cgttattttc aacgatggct acggtgtgaa atctgagaag atcgacaatg
     8581 aagaagtgtt gatcatgtcc gaaagcgaca ttctggcaat tgttgaagcg taatccgcgc
     8641 acgacactga acatacgaat ttaaggaata aagataatgg cagctaaaga cgtaaaattc
     8701 ggtaacgacg ctcgtgtgaa aatgctgcgc ggcgtaaacg tactggcaga tgcagtgaaa
     8761 gttaccctcg gtccaaaagg ccgtaacgta gttctggata aatctttcgg tgcaccgacc
     8821 atcaccaaag atggtgtttc cgttgctcgt gaaatcgaac tggaagacaa gttcgaaaat
     8881 atgggtgcgc agatggtgaa agaagttgcc tctaaagcaa acgacgctgc aggcgacggt
     8941 accaccactg caaccgtact ggctcaggct atcatcactg aaggtctgaa agctgttgct
     9001 gcgggcatga acccgatgga cctgaaacgt ggtatcgaca aagcggttac cgctgcagtt
     9061 gaagaactga aagcgctgtc cgtaccatgc tctgactcta aagcgattgc tcaggttggt
     9121 accatctccg ctaactccga cgaaaccgta ggtaaactga tcgctgaagc gatggacaaa
     9181 gtcggtaaag aaggcgttat caccgttgaa gacggtaccg gtctgcagga cgaactggac
     9241 gtggttgaag gtatgcagtt cgaccgtggc tacctgtctc cttacttcat caacaagccg
     9301 gaaactggcg cagtagaact ggaaagcccg ttcatcctgc tggctgacaa gaaaatctcc
     9361 aacatccgcg aaatgctgcc ggttctggaa gctgttgcca aagcaggcaa accgctgctg
     9421 atcatcgctg aagatgtaga aggcgaagcg ctggcaactc tggttgttaa caccatgcgt
     9481 ggcatcgtga aagtcgctgc ggttaaagca ccgggcttcg gcgatcgtcg taaagctatg
     9541 ctgcaggata tcgcaaccct gactggcggt accgtgatct ctgaagagat cggtatggag
     9601 ctggaaaaag caaccctgga agacctgggt caggctaaac gtgttgtgat caacaaagac
     9661 accaccacta tcatcgatgg cgtgggtgaa gaagctgcaa tccagggccg tgttgctcag
     9721 atccgtcagc agattgaaga agcaacttct gactacgacc gtgaaaaact gcaggaacgc
     9781 gtagcgaaac tggcaggcgg cgttgcagtt atcaaagtgg gtgctgctac cgaagttgaa
     9841 atgaaagaga aaaaagcacg cgttgaagat gccctgcacg cgacccgtgc tgcggtagaa
     9901 gaaggcgtgg ttgctggtgg tggtgttgcg ctgatccgcg tagcgtctaa actggctgac
     9961 ctgcgtggtc agaacgaaga ccagaacgtg ggtatcaaag ttgcactgcg tgcaatggaa
    10021 gctccgctgc gtcagatcgt attgaactgc ggcgaagaac cgtctgttgt tgctaacacc
    10081 gttaaaggcg gcgacggcaa ctacggttac aacgcagcaa ccgaagaata cggcaacatg
    10141 atcgacatgg gtatcctgga tccaaccaaa gtaactcgtt ctgctctgca gtacgcagct
    10201 tctgtggctg gcctgatgat caccaccgaa tgcatggtta ccgacctgcc gaaaaacgat
    10261 gcagctgact taggcgctgc tggcggtatg ggcggcatgg gtggcatggg cggcatgatg
    10321 taattgccct gcacctcgca gaaataaaca aacccccggg cagaaatgtc tgggggtttt
    10381 tcttttggtc atctttctag tataagattc agacacggac gacgcgagtg gcgcctcgac
    10441 tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
    10501 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagac ctagacttca
    10561 ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag cgctgatgtc
    10621 cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc tgaacaggag ggacagctga
    10681 tagaaacaga agccactgga gcacctcaaa aacaccatca tacactaaat cagtaagttg
    10741 gcagcatcac ccgacgcact ttgcgccgaa taaatacctg tgacggaaga tcacttcgca
    10801 gaataaataa atcctggtgt ccctgttgat accgggaagc cctgggccaa cttttggcga
    10861 aaatgagacg ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca
    10921 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag
    10981 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
    11041 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
    11101 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
    11161 cttgcccgcc tgatgaatgc tcatccggaa ttc
//
