LOCUS       pFusionRed-B.        4129 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing the monomeric red fluorescent protein 
            FusionRed in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    pFusionRed-B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Evrogen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             114..116
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             147..842
                     /label=FusionRed
                     /note="monomeric red fluorescent protein optimized for use
                     as a fusion tag (Shemiakina et al., 2012)"
     terminator      875..969
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1013..1669
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1737..1823
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1979..2119
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2305..2893)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3067..3924)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3925..4029)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttgataaaga ggagaaatta actatgagag
      121 gatcgggatc cttaccggtc gccaccatgg tgagcgagct gattaaggag aacatgccca
      181 tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca tccgagggcg
      241 aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtcgtcgag ggcggccctc
      301 tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaga accttcatca
      361 agcaccctcc gggcatcccc gacttcttta agcagtcctt ccctgagggc ttcacatggg
      421 agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
      481 aggacggctg cctcatctac aacgtcaagg ttagaggggt gaacttccca gccaacggcc
      541 ctgtgatgca gaagaaaaca ctcggctggg aggcctccac cgagacgatg taccccgctg
      601 acggcggcct ggaaggcgca tgtgacatgg ccctgaagct cgtgggcggg ggccacctga
      661 tctgcaacct tgagaccaca tacagatcca agaaacccgc tacgaacctc aagatgcccg
      721 gcgtctacaa cgtggaccac agactggaaa gaatcaagga ggccgacgat gagacctacg
      781 tcgagcagca cgaggtggct gtggccagat actctactgg tggcgctggt gatggaggta
      841 aatgagcggc cgcaagctta attagctgag cttggactcc tgttgataga tccagtaatg
      901 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      961 ggtgagaatc caagctagct tggcgagatt ttcaggagct aaggaagcta aaatggagaa
     1021 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     1081 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     1141 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     1201 tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt
     1261 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     1321 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     1381 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     1441 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     1501 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     1561 gctgatgccg ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag
     1621 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta
     1681 aggcagttat tggtgccctt aaacgcctgg ggtaatgact ctctagcttg aggcatcaaa
     1741 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1801 acgctctcct gagtaggaca aatccgccgc tctagagctg cctcgcgcgt ttcggtgatg
     1861 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     1921 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     1981 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     2041 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     2101 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2161 ctgtcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2221 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2281 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2341 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2401 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2461 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     2521 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2581 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2641 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2701 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     2761 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2821 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2881 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2941 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3001 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3061 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3121 catagctgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3181 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3241 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3301 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3361 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     3421 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     3481 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     3541 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     3601 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     3661 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     3721 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     3781 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     3841 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     3901 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     3961 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4021 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     4081 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcac
//
