LOCUS       40924_20471        9848 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFosill-4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Williams LJ, Tabbaa DG, Li N, Berlin AM, Shea TP, Maccallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A.
  TITLE     Paired-end sequencing of Fosmid libraries by Illumina
  JOURNAL   Genome Res. 22 (11), 2241-2249 (2012)
  PUBMED    22800726
REFERENCE   2  (bases 1 to 9848)
  AUTHORS   Williams LJS., Tabbaa DG, Li N, Berlin AM, Shea TP, MacCallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2012) MBRD, GSAP, Broad Institute of MIT and 
            Harvard, 320 Charles Street, Cambridge, MA 02141, USA
REFERENCE   3  (bases 1 to 9848)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9848)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2012"; volume: "22"; issue: "11"; pages: "2241-2249"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-MAY-2012) MBRD, GSAP, Broad Institute of MIT and Harvard, 320 
            Charles Street, Cambridge, MA 02141, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..42
                     /note="SBS12 Illumina sequence; designed for amplification
                     of Illumina fosmid library"
     promoter        complement(56..73)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      57..73
                     /note="SP6 promoter; transcript counterclockwise"
                     /regulatory_class="promoter"
     CDS             complement(216..872)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(873..975)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      1814..1880
                     /note="ori2; oriS"
     rep_origin      1899..2118
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             2209..2961
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     misc_feature    2967..3217
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             3543..4715
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             4718..5686
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    5762..6235
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    6494..7164
                     /label=lambda cos site
                     /note="lambda cos site"
     misc_feature    6495..6894
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     promoter        7301..7405
                     /label=AmpR promoter
     CDS             7406..8263
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      8437..9025
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9296..9694)
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(9712..9745)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        9761..9779
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     9796..9828
                     /note="SBS3 Illumina sequence; designed for amplification
                     of Illumina fosmid library"
ORIGIN
        1 tccgatacag atcggaagag cacacgtctg aactccagtc acatcctcag cgatcctata
       61 gtgtcaccta aatcgtatgc ggccgcccgg gccgtcgacc aattctcatg tttgacagct
      121 tatcatcgaa tttctgccat tcatccgctt attatcactt attcaggcgt agcaccaggc
      181 gtttaagggc accaataact gccttaaaaa aattacgccc cgccctgcca ctcatcgcag
      241 tactgttgta attcattaag cattctgccg acatggaagc catcacagac ggcatgatga
      301 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
      361 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
      421 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc
      481 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
      541 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
      601 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc
      661 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
      721 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
      781 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
      841 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
      901 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
      961 cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca
     1021 acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg
     1081 cgataagctc atggagcggc gtaaccgtcg cacaggaagg acagagaaag cgcggatctg
     1141 ggaagtgacg gacagaacgg tcaggacctg gattggggag gcggttgccg ccgctgctgc
     1201 tgacggtgtg acgttctctg ttccggtcac accacatacg ttccgccatt cctatgcgat
     1261 gcacatgctg tatgccggta taccgctgaa agttctgcaa agcctgatgg gacataagtc
     1321 catcagttca acggaagtct acacgaaggt ttttgcgctg gatgtggctg cccggcaccg
     1381 ggtgcagttt gcgatgccgg agtctgatgc ggttgcgatg ctgaaacaat tatcctgaga
     1441 ataaatgcct tggcctttat atggaaatgt ggaactgagt ggatatgctg tttttgtctg
     1501 ttaaacagag aagctggctg ttatccactg agaagcgaac gaaacagtcg ggaaaatctc
     1561 ccattatcgt agagatccgc attattaatc tcaggagcct gtgtagcgtt tataggaagt
     1621 agtgttctgt catgatgcct gcaagcggta acgaaaacga tttgaatatg ccttcaggaa
     1681 caatagaaat cttcgtgcgg tgttacgttg aagtggagcg gattatgtca gcaatggaca
     1741 gaacaaccta atgaacacag aaccatgatg tggtctgtcc ttttacagcc agtaggctcg
     1801 ccgcagtcga gcgacggcga agccctcgag tgagcgagga agcaccaggg aacagcactt
     1861 atatattctg cttacacacg atgcctgaaa aaacttccct tggggttatc cacttatcca
     1921 cggggatatt tttataatta ttttttttat agtttttaga tcttcttttt tagagcgcct
     1981 tgtaggcctt tatccatgct ggttctagag aaggtgttgt gacaaattgc cctttcagtg
     2041 tgacaaatca ccctcaaatg acagtcctgt ctgtgacaaa ttgcccttaa ccctgtgaca
     2101 aattgccctc agaagaagct gttttttcac aaagttatcc ctgcttattg actctttttt
     2161 atttagtgtg acaatctaaa aacttgtcac acttcacatg gatctgtcat ggcggaaaca
     2221 gcggttatca atcacaagaa acgtaaaaat agcccgcgaa tcgtccagtc aaacgacctc
     2281 actgaggcgg catatagtct ctcccgggat caaaaacgta tgctgtatct gttcgttgac
     2341 cagatcagaa aatctgatgg caccctacag gaacatgacg gtatctgcga gatccatgtt
     2401 gctaaatatg ctgaaatatt cggattgacc tctgcggaag ccagtaagga tatacggcag
     2461 gcattgaaga gtttcgcggg gaaggaagtg gttttttatc gccctgaaga ggatgccggc
     2521 gatgaaaaag gctatgaatc ttttccttgg tttatcaaac gtgcgcacag tccatccaga
     2581 gggctttaca gtgtacatat caacccatat ctcattccct tctttatcgg gttacagaac
     2641 cggtttacgc agtttcggct tagtgaaaca aaagaaatca ccaatccgta tgccatgcgt
     2701 ttatacgaat ccctgtgtca gtatcgtaag ccggatggct caggcatcgt ctctctgaaa
     2761 atcgactgga tcatagagcg ttaccagctg cctcaaagtt accagcgtat gcctgacttc
     2821 cgccgccgct tcctgcaggt ctgtgttaat gagatcaaca gcagaactcc aatgcgcctc
     2881 tcatacattg agaaaaagaa aggccgccag acgactcata tcgtattttc cttccgcgat
     2941 atcacttcca tgacgacagg atagtctgag ggttatctgt cacagatttg agggtggttc
     3001 gtcacatttg ttctgaccta ctgagggtaa tttgtcacag ttttgctgtt tccttcagcc
     3061 tgcatggatt ttctcatact ttttgaactg taatttttaa ggaagccaaa tttgagggca
     3121 gtttgtcaca gttgatttcc ttctctttcc cttcgtcatg tgacctgata tcgggggtta
     3181 gttcgtcatc attgatgagg gttgattatc acagtttatt actctgaatt ggctatccgc
     3241 gtgtgtacct ctacctggag tttttcccac ggtggatatt tcttcttgcg ctgagcgtaa
     3301 gagctatctg acagaacagt tcttctttgc ttcctcgcca gttcgctcgc tatgctcggt
     3361 tacacggctg cggcgagcgc tagtgataat aagtgactga ggtatgtgct cctcttatct
     3421 ccttttgtag tgttgctctt attttaaaca actttgcggt tttttgatga ctttgcgatt
     3481 ttgttgttgc tttgcagtaa attgcaagat ttaataaaaa aacgcaaagc aatgattaaa
     3541 ggatgttcag aatgaaactc atggaaacac ttaaccagtg cataaacgct ggtcatgaaa
     3601 tgacgaaggc tatcgccatt gcacagttta atgatgacag cccggaagcg aggaaaataa
     3661 cccggcgctg gagaataggt gaagcagcgg atttagttgg ggtttcttct caggctatca
     3721 gagatgccga gaaagcaggg cgactaccgc acccggatat ggaaattcga ggacgggttg
     3781 agcaacgagt tggttataca attgaacaaa ttaatcatat gcgtgatgtg tttggtacgc
     3841 gattgcgacg tgctgaagac gtatttccac cggtgatcgg ggttgctgct cataaaggtg
     3901 gcgtttacaa aacctcagtt tctgttcatc ttgctcagga tctggctctg aaggggctac
     3961 gagttttgct cgtggaaggt aacgaccccc agggaacagc ctcaatgtat cacggatggg
     4021 taccagatct tcatattcat gcagaagaca ctctcctgcc tttctatctt ggggaaaagg
     4081 acgatgtcac ttatgcaata aagcccactt gctggccggg gcttgacatt attccttcct
     4141 gtctggctct gcaccgtatt gaaactgagt taatgggcaa atttgatgaa ggtaaactgc
     4201 ccaccgatcc acacctgatg ctccgactgg ccattgaaac tgttgctcat gactatgatg
     4261 tcatagttat tgacagcgca cctaacctgg gtatcggcac gattaatgtc gtatgtgctg
     4321 ctgatgtgct gattgttccc acgcctgctg agttgtttga ctacacctcc gcactgcagt
     4381 ttttcgatat gcttcgtgat ctgctcaaga acgttgatct taaagggttc gagcctgatg
     4441 tacgtatttt gcttaccaaa tacagcaata gtaatggctc tcagtccccg tggatggagg
     4501 agcaaattcg ggatgcctgg ggaagcatgg ttctaaaaaa tgttgtacgt gaaacggatg
     4561 aagttggtaa aggtcagatc cggatgagaa ctgtttttga acaggccatt gatcaacgct
     4621 cctcaactgg tgcctggaga aatgctcttt ctatttggga acctgtctgc aatgaaattt
     4681 tcgatcgtct gattaaacca cgctgggaga ttagataatg aagcgtgcgc ctgttattcc
     4741 aaaacatacg ctcaatactc aaccggttga agatacttcg ttatcgacac cagctgcccc
     4801 gatggtggat tcgttaattg cgcgcgtagg agtaatggct cgcggtaatg ccattacttt
     4861 gcctgtatgt ggtcgggatg tgaagtttac tcttgaagtg ctccggggtg atagtgttga
     4921 gaagacctct cgggtatggt caggtaatga acgtgaccag gagctgctta ctgaggacgc
     4981 actggatgat ctcatccctt cttttctact gactggtcaa cagacaccgg cgttcggtcg
     5041 aagagtatct ggtgtcatag aaattgccga tgggagtcgc cgtcgtaaag ctgctgcact
     5101 taccgaaagt gattatcgtg ttctggttgg cgagctggat gatgagcaga tggctgcatt
     5161 atccagattg ggtaacgatt atcgcccaac aagtgcttat gaacgtggtc agcgttatgc
     5221 aagccgattg cagaatgaat ttgctggaaa tatttctgcg ctggctgatg cggaaaatat
     5281 ttcacgtaag attattaccc gctgtatcaa caccgccaaa ttgcctaaat cagttgttgc
     5341 tcttttttct caccccggtg aactatctgc ccggtcaggt gatgcacttc aaaaagcctt
     5401 tacagataaa gaggaattac ttaagcagca ggcatctaac cttcatgagc agaaaaaagc
     5461 tggggtgata tttgaagctg aagaagttat cactctttta acttctgtgc ttaaaacgtc
     5521 atctgcatca agaactagtt taagctcacg acatcagttt gctcctggag cgacagtatt
     5581 gtataagggc gataaaatgg tgcttaacct ggacaggtct cgtgttccaa ctgagtgtat
     5641 agagaaaatt gaggccattc ttaaggaact tgaaaagcca gcaccctgat gcgaccacgt
     5701 tttagtctac gtttatctgt ctttacttaa tgtcctttgt tacaggccag aaagcataac
     5761 tggcctgaat attctctctg ggcccactgt tccacttgta tcgtcggtct gataatcaga
     5821 ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacggtccca
     5881 ctcgtatcgt cggtctgatt attagtctgg gaccacggtc ccactcgtat cgtcggtctg
     5941 ataatcagac tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc
     6001 atggtcccac tcgtatcgtc ggtctgatta ttagtctggg accacggtcc cactcgtatc
     6061 gtcggtctga ttattagtct ggaaccacgg tcccactcgt atcgtcggtc tgattattag
     6121 tctgggacca cggtcccact cgtatcgtcg gtctgattat tagtctggga ccacgatccc
     6181 actcgtgttg tcggtctgat tatcggtctg ggaccacggt cccacttgta ttgtcgatca
     6241 gactatcagc gtgagactac gattccatca atgcctgtca agggcaagta ttgacatgtc
     6301 gtcgtaacct gtagaacgga gtaacctcgg tgtgcggttg tatgcctgct gtggattgct
     6361 gctgtgtcct gcttatccac aacattttgc gcacggttat gtggacaaaa tacctggtta
     6421 cccaggccgt gccggcacgt taaccgggct gcatccgatg caagtgtgtc gctgtcgacg
     6481 agctcgcgag ctcggacatg aggttgcccc gtattcagtg tcgctgattt gtattgtctg
     6541 aagttgtttt tacgttaagt tgatgcagat caattaatac gatacctgcg tcataattga
     6601 ttatttgacg tggtttgatg gcctccacgc acgttgtgat atgtagatga taatcattat
     6661 cactttacgg gtcctttccg gtgatccgac aggttacggg gcggcgacct cgcgggtttt
     6721 cgctatttat gaaaattttc cggtttaagg cgtttccgtt cttcttcgtc ataacttaat
     6781 gtttttattt aaaataccct ctgaaaagaa aggaaacgac aggtgctgaa agcgaggctt
     6841 tttggcctct gtcgtttcct ttctctgttt ttgtccgtgg aatgaacaat ggaagtcctg
     6901 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6961 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     7021 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     7081 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     7141 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     7201 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     7261 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     7321 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7381 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     7441 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7501 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     7561 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     7621 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     7681 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     7741 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     7801 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     7861 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     7921 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7981 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     8041 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     8101 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     8161 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     8221 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     8281 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     8341 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     8401 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     8461 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8521 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8581 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8641 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     8701 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8761 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8821 gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8881 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8941 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     9001 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     9061 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     9121 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     9181 cgagtcagtg agcgaggaag cggaggagcg cccaatacgc aaaccgcctc tgtttaaacg
     9241 ttggccgatt cattaatgca gcttgcgccg ttccacttcg atgcgtcagt gaagcgacat
     9301 gaggcgcgcc cgtattcagt gtcgctgatt tgtattgtct gaagttgttt ttacgttaag
     9361 ttgaagcaga tcaattaata cgatacctgc gtcataattg attatttgac gtggtttgat
     9421 ggcctccacg cacgttgtga tatgtagaag ataatcatta tcactttacg ggtcctttcc
     9481 ggtgatccga caggttacgg ggcggcgacc tcgcgggttt tcgctattta tgaaaatttt
     9541 ccggtttaag gcgtttccgt tcttcttcgt cataacttaa tgaatttatt taaaataccc
     9601 tctgaaaaga aaggaaacga caggtgctga aagcgagctt tttggcctct gtcgtttcct
     9661 ttctctgttt ttgtccgtgg aatgaacaat ggaagtccga gctcatcgct aataacttcg
     9721 tatagcatac attatacgaa gttatattcg atgcggccgc taatacgact cactataggg
     9781 agaagcttgc tgaggacact ctttccctac acgacgctct tccgatcttc atccacgtgc
     9841 atgctgga
//
