LOCUS       40924_20466        9863 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFosill-3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Williams LJ, Tabbaa DG, Li N, Berlin AM, Shea TP, Maccallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A.
  TITLE     Paired-end sequencing of Fosmid libraries by Illumina
  JOURNAL   Genome Res. 22 (11), 2241-2249 (2012)
  PUBMED    22800726
REFERENCE   2  (bases 1 to 9863)
  AUTHORS   Williams LJS., Tabbaa DG, Li N, Berlin AM, Shea TP, MacCallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2012) MBRD, GSAP, Broad Institute of MIT and 
            Harvard, 320 Charles Street, Cambridge, MA 02141, USA
REFERENCE   3  (bases 1 to 9863)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9863)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2012"; volume: "22"; issue: "11"; pages: "2241-2249"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-MAY-2012) MBRD, GSAP, Broad Institute of MIT and Harvard, 320 
            Charles Street, Cambridge, MA 02141, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..60
                     /note="SBS8 Illumina sequence; designed for amplification
                     of Illumina fosmid library"
     promoter        complement(71..88)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      72..88
                     /note="SP6 promoter; transcript counterclockwise"
                     /regulatory_class="promoter"
     CDS             complement(231..887)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(888..990)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      1829..1895
                     /note="ori2; oriS"
     rep_origin      1914..2133
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             2224..2976
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     misc_feature    2982..3232
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             3558..4730
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             4733..5701
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    5777..6250
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    6509..7179
                     /label=lambda cos site
                     /note="lambda cos site"
     misc_feature    6510..6909
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     promoter        7316..7420
                     /label=AmpR promoter
     CDS             7421..8278
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      8452..9040
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9311..9709)
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(9727..9760)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        9776..9794
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     9811..9843
                     /note="SBS3 Illumina sequence; designed for amplification
                     of Illumina fosmid library"
ORIGIN
        1 tcctacctca ttcgtgatac agatcggaag agcggttcag caggaatgcc gagaccgatc
       61 ctcagcgatc ctatagtgtc acctaaatcg tatgcggccg cccgggccgt cgaccaattc
      121 tcatgtttga cagcttatca tcgaatttct gccattcatc cgcttattat cacttattca
      181 ggcgtagcac caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc
      241 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
      301 cagacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
      361 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
      421 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
      481 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
      541 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
      601 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
      661 gccatacgga attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
      721 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
      781 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
      841 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
      901 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
      961 tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccaaaa gttggcccag
     1021 ggcttcccgg tatcaacagg gacaccagga tttatttatt ctgcgaagtg atcttccgtc
     1081 acaggtattt attcgcgata agctcatgga gcggcgtaac cgtcgcacag gaaggacaga
     1141 gaaagcgcgg atctgggaag tgacggacag aacggtcagg acctggattg gggaggcggt
     1201 tgccgccgct gctgctgacg gtgtgacgtt ctctgttccg gtcacaccac atacgttccg
     1261 ccattcctat gcgatgcaca tgctgtatgc cggtataccg ctgaaagttc tgcaaagcct
     1321 gatgggacat aagtccatca gttcaacgga agtctacacg aaggtttttg cgctggatgt
     1381 ggctgcccgg caccgggtgc agtttgcgat gccggagtct gatgcggttg cgatgctgaa
     1441 acaattatcc tgagaataaa tgccttggcc tttatatgga aatgtggaac tgagtggata
     1501 tgctgttttt gtctgttaaa cagagaagct ggctgttatc cactgagaag cgaacgaaac
     1561 agtcgggaaa atctcccatt atcgtagaga tccgcattat taatctcagg agcctgtgta
     1621 gcgtttatag gaagtagtgt tctgtcatga tgcctgcaag cggtaacgaa aacgatttga
     1681 atatgccttc aggaacaata gaaatcttcg tgcggtgtta cgttgaagtg gagcggatta
     1741 tgtcagcaat ggacagaaca acctaatgaa cacagaacca tgatgtggtc tgtcctttta
     1801 cagccagtag gctcgccgca gtcgagcgac ggcgaagccc tcgagtgagc gaggaagcac
     1861 cagggaacag cacttatata ttctgcttac acacgatgcc tgaaaaaact tcccttgggg
     1921 ttatccactt atccacgggg atatttttat aattattttt tttatagttt ttagatcttc
     1981 ttttttagag cgccttgtag gcctttatcc atgctggttc tagagaaggt gttgtgacaa
     2041 attgcccttt cagtgtgaca aatcaccctc aaatgacagt cctgtctgtg acaaattgcc
     2101 cttaaccctg tgacaaattg ccctcagaag aagctgtttt ttcacaaagt tatccctgct
     2161 tattgactct tttttattta gtgtgacaat ctaaaaactt gtcacacttc acatggatct
     2221 gtcatggcgg aaacagcggt tatcaatcac aagaaacgta aaaatagccc gcgaatcgtc
     2281 cagtcaaacg acctcactga ggcggcatat agtctctccc gggatcaaaa acgtatgctg
     2341 tatctgttcg ttgaccagat cagaaaatct gatggcaccc tacaggaaca tgacggtatc
     2401 tgcgagatcc atgttgctaa atatgctgaa atattcggat tgacctctgc ggaagccagt
     2461 aaggatatac ggcaggcatt gaagagtttc gcggggaagg aagtggtttt ttatcgccct
     2521 gaagaggatg ccggcgatga aaaaggctat gaatcttttc cttggtttat caaacgtgcg
     2581 cacagtccat ccagagggct ttacagtgta catatcaacc catatctcat tcccttcttt
     2641 atcgggttac agaaccggtt tacgcagttt cggcttagtg aaacaaaaga aatcaccaat
     2701 ccgtatgcca tgcgtttata cgaatccctg tgtcagtatc gtaagccgga tggctcaggc
     2761 atcgtctctc tgaaaatcga ctggatcata gagcgttacc agctgcctca aagttaccag
     2821 cgtatgcctg acttccgccg ccgcttcctg caggtctgtg ttaatgagat caacagcaga
     2881 actccaatgc gcctctcata cattgagaaa aagaaaggcc gccagacgac tcatatcgta
     2941 ttttccttcc gcgatatcac ttccatgacg acaggatagt ctgagggtta tctgtcacag
     3001 atttgagggt ggttcgtcac atttgttctg acctactgag ggtaatttgt cacagttttg
     3061 ctgtttcctt cagcctgcat ggattttctc atactttttg aactgtaatt tttaaggaag
     3121 ccaaatttga gggcagtttg tcacagttga tttccttctc tttcccttcg tcatgtgacc
     3181 tgatatcggg ggttagttcg tcatcattga tgagggttga ttatcacagt ttattactct
     3241 gaattggcta tccgcgtgtg tacctctacc tggagttttt cccacggtgg atatttcttc
     3301 ttgcgctgag cgtaagagct atctgacaga acagttcttc tttgcttcct cgccagttcg
     3361 ctcgctatgc tcggttacac ggctgcggcg agcgctagtg ataataagtg actgaggtat
     3421 gtgctcctct tatctccttt tgtagtgttg ctcttatttt aaacaacttt gcggtttttt
     3481 gatgactttg cgattttgtt gttgctttgc agtaaattgc aagatttaat aaaaaaacgc
     3541 aaagcaatga ttaaaggatg ttcagaatga aactcatgga aacacttaac cagtgcataa
     3601 acgctggtca tgaaatgacg aaggctatcg ccattgcaca gtttaatgat gacagcccgg
     3661 aagcgaggaa aataacccgg cgctggagaa taggtgaagc agcggattta gttggggttt
     3721 cttctcaggc tatcagagat gccgagaaag cagggcgact accgcacccg gatatggaaa
     3781 ttcgaggacg ggttgagcaa cgagttggtt atacaattga acaaattaat catatgcgtg
     3841 atgtgtttgg tacgcgattg cgacgtgctg aagacgtatt tccaccggtg atcggggttg
     3901 ctgctcataa aggtggcgtt tacaaaacct cagtttctgt tcatcttgct caggatctgg
     3961 ctctgaaggg gctacgagtt ttgctcgtgg aaggtaacga cccccaggga acagcctcaa
     4021 tgtatcacgg atgggtacca gatcttcata ttcatgcaga agacactctc ctgcctttct
     4081 atcttgggga aaaggacgat gtcacttatg caataaagcc cacttgctgg ccggggcttg
     4141 acattattcc ttcctgtctg gctctgcacc gtattgaaac tgagttaatg ggcaaatttg
     4201 atgaaggtaa actgcccacc gatccacacc tgatgctccg actggccatt gaaactgttg
     4261 ctcatgacta tgatgtcata gttattgaca gcgcacctaa cctgggtatc ggcacgatta
     4321 atgtcgtatg tgctgctgat gtgctgattg ttcccacgcc tgctgagttg tttgactaca
     4381 cctccgcact gcagtttttc gatatgcttc gtgatctgct caagaacgtt gatcttaaag
     4441 ggttcgagcc tgatgtacgt attttgctta ccaaatacag caatagtaat ggctctcagt
     4501 ccccgtggat ggaggagcaa attcgggatg cctggggaag catggttcta aaaaatgttg
     4561 tacgtgaaac ggatgaagtt ggtaaaggtc agatccggat gagaactgtt tttgaacagg
     4621 ccattgatca acgctcctca actggtgcct ggagaaatgc tctttctatt tgggaacctg
     4681 tctgcaatga aattttcgat cgtctgatta aaccacgctg ggagattaga taatgaagcg
     4741 tgcgcctgtt attccaaaac atacgctcaa tactcaaccg gttgaagata cttcgttatc
     4801 gacaccagct gccccgatgg tggattcgtt aattgcgcgc gtaggagtaa tggctcgcgg
     4861 taatgccatt actttgcctg tatgtggtcg ggatgtgaag tttactcttg aagtgctccg
     4921 gggtgatagt gttgagaaga cctctcgggt atggtcaggt aatgaacgtg accaggagct
     4981 gcttactgag gacgcactgg atgatctcat cccttctttt ctactgactg gtcaacagac
     5041 accggcgttc ggtcgaagag tatctggtgt catagaaatt gccgatggga gtcgccgtcg
     5101 taaagctgct gcacttaccg aaagtgatta tcgtgttctg gttggcgagc tggatgatga
     5161 gcagatggct gcattatcca gattgggtaa cgattatcgc ccaacaagtg cttatgaacg
     5221 tggtcagcgt tatgcaagcc gattgcagaa tgaatttgct ggaaatattt ctgcgctggc
     5281 tgatgcggaa aatatttcac gtaagattat tacccgctgt atcaacaccg ccaaattgcc
     5341 taaatcagtt gttgctcttt tttctcaccc cggtgaacta tctgcccggt caggtgatgc
     5401 acttcaaaaa gcctttacag ataaagagga attacttaag cagcaggcat ctaaccttca
     5461 tgagcagaaa aaagctgggg tgatatttga agctgaagaa gttatcactc ttttaacttc
     5521 tgtgcttaaa acgtcatctg catcaagaac tagtttaagc tcacgacatc agtttgctcc
     5581 tggagcgaca gtattgtata agggcgataa aatggtgctt aacctggaca ggtctcgtgt
     5641 tccaactgag tgtatagaga aaattgaggc cattcttaag gaacttgaaa agccagcacc
     5701 ctgatgcgac cacgttttag tctacgttta tctgtcttta cttaatgtcc tttgttacag
     5761 gccagaaagc ataactggcc tgaatattct ctctgggccc actgttccac ttgtatcgtc
     5821 ggtctgataa tcagactggg accacggtcc cactcgtatc gtcggtctga ttattagtct
     5881 gggaccacgg tcccactcgt atcgtcggtc tgattattag tctgggacca cggtcccact
     5941 cgtatcgtcg gtctgataat cagactggga ccacggtccc actcgtatcg tcggtctgat
     6001 tattagtctg ggaccatggt cccactcgta tcgtcggtct gattattagt ctgggaccac
     6061 ggtcccactc gtatcgtcgg tctgattatt agtctggaac cacggtccca ctcgtatcgt
     6121 cggtctgatt attagtctgg gaccacggtc ccactcgtat cgtcggtctg attattagtc
     6181 tgggaccacg atcccactcg tgttgtcggt ctgattatcg gtctgggacc acggtcccac
     6241 ttgtattgtc gatcagacta tcagcgtgag actacgattc catcaatgcc tgtcaagggc
     6301 aagtattgac atgtcgtcgt aacctgtaga acggagtaac ctcggtgtgc ggttgtatgc
     6361 ctgctgtgga ttgctgctgt gtcctgctta tccacaacat tttgcgcacg gttatgtgga
     6421 caaaatacct ggttacccag gccgtgccgg cacgttaacc gggctgcatc cgatgcaagt
     6481 gtgtcgctgt cgacgagctc gcgagctcgg acatgaggtt gccccgtatt cagtgtcgct
     6541 gatttgtatt gtctgaagtt gtttttacgt taagttgatg cagatcaatt aatacgatac
     6601 ctgcgtcata attgattatt tgacgtggtt tgatggcctc cacgcacgtt gtgatatgta
     6661 gatgataatc attatcactt tacgggtcct ttccggtgat ccgacaggtt acggggcggc
     6721 gacctcgcgg gttttcgcta tttatgaaaa ttttccggtt taaggcgttt ccgttcttct
     6781 tcgtcataac ttaatgtttt tatttaaaat accctctgaa aagaaaggaa acgacaggtg
     6841 ctgaaagcga ggctttttgg cctctgtcgt ttcctttctc tgtttttgtc cgtggaatga
     6901 acaatggaag tcctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     6961 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     7021 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     7081 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     7141 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     7201 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     7261 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     7321 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7381 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7441 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7501 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7561 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7621 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7681 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7741 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7801 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7861 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7921 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7981 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8041 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8101 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8161 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8221 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8281 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8341 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8401 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8461 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8521 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8581 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8641 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8701 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8761 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8821 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
     8881 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8941 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     9001 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9061 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     9121 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     9181 aacgaccgag cgcagcgagt cagtgagcga ggaagcggag gagcgcccaa tacgcaaacc
     9241 gcctctgttt aaacgttggc cgattcatta atgcagcttg cgccgttcca cttcgatgcg
     9301 tcagtgaagc gacatgaggc gcgcccgtat tcagtgtcgc tgatttgtat tgtctgaagt
     9361 tgtttttacg ttaagttgaa gcagatcaat taatacgata cctgcgtcat aattgattat
     9421 ttgacgtggt ttgatggcct ccacgcacgt tgtgatatgt agaagataat cattatcact
     9481 ttacgggtcc tttccggtga tccgacaggt tacggggcgg cgacctcgcg ggttttcgct
     9541 atttatgaaa attttccggt ttaaggcgtt tccgttcttc ttcgtcataa cttaatgaat
     9601 ttatttaaaa taccctctga aaagaaagga aacgacaggt gctgaaagcg agctttttgg
     9661 cctctgtcgt ttcctttctc tgtttttgtc cgtggaatga acaatggaag tccgagctca
     9721 tcgctaataa cttcgtatag catacattat acgaagttat attcgatgcg gccgctaata
     9781 cgactcacta tagggagaag cttgctgagg acactctttc cctacacgac gctcttccga
     9841 tcttcatcca cgtgcatgct gga
//
