LOCUS       40924_20456        9905 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFosill-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Williams LJ, Tabbaa DG, Li N, Berlin AM, Shea TP, Maccallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A.
  TITLE     Paired-end sequencing of Fosmid libraries by Illumina
  JOURNAL   Genome Res. 22 (11), 2241-2249 (2012)
  PUBMED    22800726
REFERENCE   2  (bases 1 to 9905)
  AUTHORS   Williams LJS., Tabbaa DG, Li N, Berlin AM, Shea TP, MacCallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2012) MBRD, GSAP, Broad Institute of MIT and 
            Harvard, 320 Charles Street, Cambridge, MA 02141, USA
REFERENCE   3  (bases 1 to 9905)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9905)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2012"; volume: "22"; issue: "11"; pages: "2241-2249"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-MAY-2012) MBRD, GSAP, Broad Institute of MIT and Harvard, 320 
            Charles Street, Cambridge, MA 02141, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..56
                     /note="SBS8 Illumina sequence; designed for amplification
                     of Illumina fosmid library"
     promoter        complement(93..110)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      94..110
                     /note="SP6 promoter; transcript counterclockwise"
                     /regulatory_class="promoter"
     CDS             complement(253..909)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(910..1012)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      1851..1917
                     /note="ori2; oriS"
     rep_origin      1936..2155
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             2246..2998
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     misc_feature    3004..3254
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             3580..4752
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             4755..5723
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    5799..6272
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    6531..7201
                     /label=lambda cos site
                     /note="lambda cos site"
     misc_feature    6532..6931
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     promoter        7338..7442
                     /label=AmpR promoter
     CDS             7443..8300
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      8474..9062
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9333..9731)
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(9749..9782)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        9798..9816
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     9857..9889
                     /note="SBS3 Illumina sequence; designed for amplification
                     of Illumina fosmid library"
ORIGIN
        1 tccagcatgc acgtggagat cggaagagcg gttcagcagg aatgccgaga ccgatcctca
       61 gcagtgtcgt atgccgtctt ctgcttgaga tcctatagtg tcacctaaat cgtatgcggc
      121 cgcccgggcc gtcgaccaat tctcatgttt gacagcttat catcgaattt ctgccattca
      181 tccgcttatt atcacttatt caggcgtagc accaggcgtt taagggcacc aataactgcc
      241 ttaaaaaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
      301 tctgccgaca tggaagccat cacagacggc atgatgaacc tgaatcgcca gcggcatcag
      361 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
      421 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
      481 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
      541 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
      601 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
      661 caccagctca ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc
      721 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
      781 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
      841 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
      901 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
      961 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
     1021 ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta
     1081 ttctgcgaag tgatcttccg tcacaggtat ttattcgcga taagctcatg gagcggcgta
     1141 accgtcgcac aggaaggaca gagaaagcgc ggatctggga agtgacggac agaacggtca
     1201 ggacctggat tggggaggcg gttgccgccg ctgctgctga cggtgtgacg ttctctgttc
     1261 cggtcacacc acatacgttc cgccattcct atgcgatgca catgctgtat gccggtatac
     1321 cgctgaaagt tctgcaaagc ctgatgggac ataagtccat cagttcaacg gaagtctaca
     1381 cgaaggtttt tgcgctggat gtggctgccc ggcaccgggt gcagtttgcg atgccggagt
     1441 ctgatgcggt tgcgatgctg aaacaattat cctgagaata aatgccttgg cctttatatg
     1501 gaaatgtgga actgagtgga tatgctgttt ttgtctgtta aacagagaag ctggctgtta
     1561 tccactgaga agcgaacgaa acagtcggga aaatctccca ttatcgtaga gatccgcatt
     1621 attaatctca ggagcctgtg tagcgtttat aggaagtagt gttctgtcat gatgcctgca
     1681 agcggtaacg aaaacgattt gaatatgcct tcaggaacaa tagaaatctt cgtgcggtgt
     1741 tacgttgaag tggagcggat tatgtcagca atggacagaa caacctaatg aacacagaac
     1801 catgatgtgg tctgtccttt tacagccagt aggctcgccg cagtcgagcg acggcgaagc
     1861 cctcgagtga gcgaggaagc accagggaac agcacttata tattctgctt acacacgatg
     1921 cctgaaaaaa cttcccttgg ggttatccac ttatccacgg ggatattttt ataattattt
     1981 tttttatagt ttttagatct tcttttttag agcgccttgt aggcctttat ccatgctggt
     2041 tctagagaag gtgttgtgac aaattgccct ttcagtgtga caaatcaccc tcaaatgaca
     2101 gtcctgtctg tgacaaattg cccttaaccc tgtgacaaat tgccctcaga agaagctgtt
     2161 ttttcacaaa gttatccctg cttattgact cttttttatt tagtgtgaca atctaaaaac
     2221 ttgtcacact tcacatggat ctgtcatggc ggaaacagcg gttatcaatc acaagaaacg
     2281 taaaaatagc ccgcgaatcg tccagtcaaa cgacctcact gaggcggcat atagtctctc
     2341 ccgggatcaa aaacgtatgc tgtatctgtt cgttgaccag atcagaaaat ctgatggcac
     2401 cctacaggaa catgacggta tctgcgagat ccatgttgct aaatatgctg aaatattcgg
     2461 attgacctct gcggaagcca gtaaggatat acggcaggca ttgaagagtt tcgcggggaa
     2521 ggaagtggtt ttttatcgcc ctgaagagga tgccggcgat gaaaaaggct atgaatcttt
     2581 tccttggttt atcaaacgtg cgcacagtcc atccagaggg ctttacagtg tacatatcaa
     2641 cccatatctc attcccttct ttatcgggtt acagaaccgg tttacgcagt ttcggcttag
     2701 tgaaacaaaa gaaatcacca atccgtatgc catgcgttta tacgaatccc tgtgtcagta
     2761 tcgtaagccg gatggctcag gcatcgtctc tctgaaaatc gactggatca tagagcgtta
     2821 ccagctgcct caaagttacc agcgtatgcc tgacttccgc cgccgcttcc tgcaggtctg
     2881 tgttaatgag atcaacagca gaactccaat gcgcctctca tacattgaga aaaagaaagg
     2941 ccgccagacg actcatatcg tattttcctt ccgcgatatc acttccatga cgacaggata
     3001 gtctgagggt tatctgtcac agatttgagg gtggttcgtc acatttgttc tgacctactg
     3061 agggtaattt gtcacagttt tgctgtttcc ttcagcctgc atggattttc tcatactttt
     3121 tgaactgtaa tttttaagga agccaaattt gagggcagtt tgtcacagtt gatttccttc
     3181 tctttccctt cgtcatgtga cctgatatcg ggggttagtt cgtcatcatt gatgagggtt
     3241 gattatcaca gtttattact ctgaattggc tatccgcgtg tgtacctcta cctggagttt
     3301 ttcccacggt ggatatttct tcttgcgctg agcgtaagag ctatctgaca gaacagttct
     3361 tctttgcttc ctcgccagtt cgctcgctat gctcggttac acggctgcgg cgagcgctag
     3421 tgataataag tgactgaggt atgtgctctt cttatctcct tttgtagtgt tgctcttatt
     3481 ttaaacaact ttgcggtttt ttgatgactt tgcgattttg ttgttgcttt gcagtaaatt
     3541 gcaagattta ataaaaaaac gcaaagcaat gattaaagga tgttcagaat gaaactcatg
     3601 gaaacactta accagtgcat aaacgctggt catgaaatga cgaaggctat cgccattgca
     3661 cagtttaatg atgacagccc ggaagcgagg aaaataaccc ggcgctggag aataggtgaa
     3721 gcagcggatt tagttggggt ttcttctcag gctatcagag atgccgagaa agcagggcga
     3781 ctaccgcacc cggatatgga aattcgagga cgggttgagc aacgtgttgg ttatacaatt
     3841 gaacaaatta atcatatgcg tgatgtgttt ggtacgcgat tgcgacgtgc tgaagacgta
     3901 tttccaccgg tgatcggggt tgctgcccat aaaggtggcg tttacaaaac ctcagtttct
     3961 gttcatcttg ctcaggatct ggctctgaag gggctacgtg ttttgctcgt ggaaggtaac
     4021 gacccccagg gaacagcctc aatgtatcac ggatgggtac cagatcttca tattcatgca
     4081 gaagacactc tcctgccttt ctatcttggg gaaaaggacg atgtcactta tgcaataaag
     4141 cccacttgct ggccggggct tgacattatt ccttcctgtc tggctctgca ccgtattgaa
     4201 actgagttaa tgggcaaatt tgatgaaggt aaactgccca ccgatccaca cctgatgctc
     4261 cgactggcca ttgaaactgt tgctcatgac tatgatgtca tagttattga cagcgcgcct
     4321 aacctgggta tcggcacgat taatgtcgta tgtgctgctg atgtgctgat tgttcccacg
     4381 cctgctgagt tgtttgacta cacctccgca ctgcagtttt tcgatatgct tcgtgatctg
     4441 ctcaagaacg ttgatcttaa agggttcgag cctgatgtac gtattttgct taccaaatac
     4501 agcaatagta atggctctca gtccccgtgg atggaggagc aaattcggga tgcctgggga
     4561 agcatggttc taaaaaatgt tgtacgtgaa acggatgaag ttggtaaagg tcagatccgg
     4621 atgagaactg tttttgaaca ggccattgat caacgctctt caactggtgc ctggagaaat
     4681 gctctttcta tttgggaacc tgtctgcaat gaaattttcg atcgtctgat taaaccacgc
     4741 tgggagatta gataatgaag cgtgcgcctg ttattccaaa acatacgctc aatactcaac
     4801 cggttgaaga tacttcgtta tcgacaccag ctgccccgat ggtggattcg ttaattgcgc
     4861 gcgtaggagt aatggctcgc ggtaatgcca ttactttgcc tgtatgtggt cgggatgtga
     4921 agtttactct tgaagtgctc cggggtgata gtgttgagaa gacctctcgg gtatggtcag
     4981 gtaatgaacg tgaccaggag ctgcttactg aggacgcact ggatgatctc atcccttctt
     5041 ttctactgac tggtcaacag acaccggcgt tcggtcgaag agtatctggt gtcatagaaa
     5101 ttgccgatgg gagtcgccgt cgtaaagctg ctgcacttac cgaaagtgat tatcgtgttc
     5161 tggttggcga gctggatgat gagcagatgg ctgcattatc cagattgggt aacgattatc
     5221 gcccaacaag tgcttatgaa cgtggtcagc gttatgcaag ccgattgcag aatgaatttg
     5281 ctggaaatat ttctgcgctg gctgatgcgg aaaatatttc acgtaagatt attacccgct
     5341 gtatcaacac cgccaaattg cctaaatcag ttgttgctct tttttctcac cccggtgaac
     5401 tatctgcccg gtcaggtgat gcacttcaaa aagcctttac agataaagag gaattactta
     5461 agcagcaggc atctaacctt catgagcaga aaaaagctgg ggtgatattt gaagctgaag
     5521 aagttatcac tcttttaact tctgtgctta aaacgtcatc tgcatcaaga actagtttaa
     5581 gctcacgaca tcagtttgct cctggagcga cagtattgta taagggcgat aaaatggtgc
     5641 ttaacctgga caggtctcgt gttccaactg agtgtataga gaaaattgag gccattctta
     5701 aggaacttga aaagccagca ccctgatgcg accacgtttt agtctacgtt tatctgtctt
     5761 tacttaatgt cctttgttac aggccagaaa gcataactgg cctgaatatt ctctctgggc
     5821 ccactgttcc acttgtatcg tcggtctgat aatcagactg ggaccacggt cccactcgta
     5881 tcgtcggtct gattattagt ctgggaccac ggtcccactc gtatcgtcgg tctgattatt
     5941 agtctgggac cacggtccca ctcgtatcgt cggtctgata atcagactgg gaccacggtc
     6001 ccactcgtat cgtcggtctg attattagtc tgggaccatg gtcccactcg tatcgtcggt
     6061 ctgattatta gtctgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctgga
     6121 accacggtcc cactcgtatc gtcggtctga ttattagtct gggaccacgg tcccactcgt
     6181 atcgtcggtc tgattattag tctgggacca cgatcccact cgtgttgtcg gtctgattat
     6241 cggtctggga ccacggtccc acttgtattg tcgatcagac tatcagcgtg agactacgat
     6301 tccatcaatg cctgtcaagg gcaagtattg acatgtcgtc gtaacctgta gaacggagta
     6361 acctcggtgt gcggttgtat gcctgctgtg gattgctgct gtgtcctgct tatccacaac
     6421 attttgcgca cggttatgtg gacaaaatac ctggttaccc aggccgtgcc ggcacgttaa
     6481 ccgggctgca tccgatgcaa gtgtgtcgct gtcgacgagc tcgcgagctc ggacatgagg
     6541 ttgccccgta ttcagtgtcg ctgatttgta ttgtctgaag ttgtttttac gttaagttga
     6601 tgcagatcaa ttaatacgat acctgcgtca taattgatta tttgacgtgg tttgatggcc
     6661 tccacgcacg ttgtgatatg tagatgataa tcattatcac tttacgggtc ctttccggtg
     6721 atccgacagg ttacggggcg gcgacctcgc gggttttcgc tatttatgaa aattttccgg
     6781 tttaaggcgt ttccgttctt cttcgtcata acttaatgtt tttatttaaa ataccctctg
     6841 aaaagaaagg aaacgacagg tgctgaaagc gaggcttttt ggcctctgtc gtttcctttc
     6901 tctgtttttg tccgtggaat gaacaatgga agtcctggcg taatagcgaa gaggcccgca
     6961 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     7021 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     7081 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     7141 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     7201 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     7261 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     7321 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7381 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7441 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7501 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7561 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7621 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7681 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7741 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7801 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7861 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7921 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7981 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8041 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8101 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8161 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8221 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8281 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8341 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8401 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8461 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8521 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8581 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     8641 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8701 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8761 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8821 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     8881 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8941 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9001 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9061 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9241 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     9301 tgcgccgttc cacttcgatg cgtcagtgaa gcgacatgag gttgccccgt attcagtgtc
     9361 gctgatttgt attgtctgaa gttgttttta cgttaagttg atgcagatca attaatacga
     9421 tacctgcgtc ataattgatt atttgacgtg gtttgatggc ctccacgcac gttgtgatat
     9481 gtagatgata atcattatca ctttacgggt cctttccggt gatccgacag gttacggggc
     9541 ggcgacctcg cgggttttcg ctatttatga aaattttccg gtttaaggcg tttccgttct
     9601 tcttcgtcat aacttaatgt ttttatttaa aataccctct gaaaagaaag gaaacgacag
     9661 gtgctgaaag cgagcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat
     9721 gaacaatgga agtccgagct catcgctaat aacttcgtat agcatacatt atacgaagtt
     9781 atattcgatg cggccgctaa tacgactcac tatagggaga agcttaatga tacggcgacc
     9841 accgacactg ctgaggacac tctttcccta cacgacgctc ttccgatctc cacgtgcatg
     9901 ctgga
//
