LOCUS       V006223                10793 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006223
VERSION     V006223
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   Barrett AR, Kang Y, Inamasu KS, Son MS, Vukovich JM, Hoang TT.
  TITLE     Genetic tools for allelic replacement in Burkholderia species
  JOURNAL   Appl. Environ. Microbiol. 74 (14), 4498-4508 (2008)
   PUBMED   18502918
REFERENCE   2  (bases 1 to 10793)
  AUTHORS   Barrett AR, Kang Y, Vukovich JM, Son MS, Hoang TT.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2007) Microbiology, University of Hawaii at Manoa,
            2538 McCarthy Mall Snyder Hall Rm. 310, Honolulu, HI 96822, USA
REFERENCE   3  (bases 1 to 10793)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10793)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2008"; volume: "74"; issue: "14";
            pages: "4498-4508"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-DEC-2007) Microbiology, University of Hawaii at Manoa, 2538
            McCarthy Mall Snyder Hall Rm. 310, Honolulu, HI 96822, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(232..583)
                     /direction=LEFT
                     /label="pRO1600 oriV"
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             597..1427
                     /label="pRO1600 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     primer_bind     1591..1607
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      1662..1703
                     /regulatory_class="promoter"
     CDS             1739..2749
                     /gene="pheS"
                     /label="Phenylalanine--tRNA ligase alpha subunit"
                     /note="Phenylalanine--tRNA ligase alpha subunit from
                     Burkholderia pseudomallei (strain 1710b). Accession#:
                     Q3JT08"
     CDS             complement(2964..4232)
                     /label="FLP"
                     /note="site-specific recombinase"
     RBS             4236..4244
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4333..5043
                     /label="lambda repressor (ts)"
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
     primer_bind     complement(5335..5351)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    5359..5375
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5383..5413)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5428..5449)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     oriT            5833..5941
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      complement(6013..6601)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6921..8036)
                     /codon_start=1
                     /gene="Pasd"
                     /product="aspartate semialdehyde dehydrogenase"
                     /label="Pasd"
                     /note="derived from Pseudomonas aeruginosa"
                     /protein_id="ABY57980.1"
                     /translation="MKRVGLIGWRGMVGSVLIQRMLEERDFDLIEPVFFTTSNVGAQAP
                     EVDKDIAPLKDAYSIDELKTLDVILTCQGGDYTSEVFPKLREAGWQGYWIDAASSLRME
                     DDAVIVLDPVNRKVIDQALDAGTRNYIGGNCTVSLMLMALGGLFDAGLVEWMSAMTYQA
                     ASGAGAQNMRDLLKQMGAAHASVADDLANPASAILDIDRKVAETLRSEAFPTEHFGAPL
                     GGSLIPWIDKELSQRRQSREEWKAQAETNKILARFKNPIPVDGICVRVGAMRCHSQALT
                     IKLNKDVPLTDIEGLIRQHNPWVKLVPNHREVSVRELTPAAVTGTLSVPVGRLRKLNMV
                     SQYLGAFTVGDQLLWGAAEPLRRMLRILLER"
     gene            complement(6921..8036)
                     /gene="Pasd"
                     /label="Pasd"
     CDS             complement(8093..9775)
                     /gene="merA"
                     /label="Mercuric reductase"
                     /note="Mercuric reductase from Pseudomonas aeruginosa.
                     Accession#: P00392"
     CDS             complement(9850..10122)
                     /gene="merP"
                     /label="Mercuric transport protein periplasmic component"
                     /note="Mercuric transport protein periplasmic component
                     from Pseudomonas aeruginosa. Accession#: P04131"
     CDS             complement(10138..10485)
                     /gene="merT"
                     /label="Mercuric transport protein MerT"
                     /note="Mercuric transport protein MerT from Pseudomonas
                     aeruginosa. Accession#: P04140"
     RBS             complement(10510..10532)
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      complement(10543..10584)
                     /regulatory_class="promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatacctc tcaggcgccg ctggtgccgc tggttggacg ccaagggtga atccgcctcg
      241 ataccctgat tactcgcttc ctgcgccctc tcaggcggcg ataggggact ggtaaaacgg
      301 ggattgccca gacgcctccc ccgccccttc aggggcacaa atgcggcccc aacggggcca
      361 cgtagtggtg cgttttttgc gtttccaccc ttttcttcct tttccctttt aaacctttta
      421 ggacgtctac aggccacgta atccgtggcc tgtagagttt aaaaagggac ggatttgttg
      481 ccattaaggg acggatttgt tgttaagaag ggacggattt gttgttgtaa agggacggat
      541 ttgttgtatt gtgggacgca gatacagtgt ccccttatac acaaggaatg tcgaacgtgg
      601 cctcaccccc aatggtttac aaaagcaatg ccctggtcga ggccgcgtat cgcctcagtg
      661 ttcaggaaca gcggatcgtt ctggcctgta ttagccaggt gaagaggagc gagcctgtca
      721 ccgatgaagt gatgtattca gtgacggcgg aggacatagc gacgatggcg ggtgtcccta
      781 tcgaatcttc ctacaaccag ctcaaagaag cggccctgcg cctgaaacgg cgggaagtcc
      841 ggttaaccca agagcccaat ggcaagggga aaagaccgag tgtgatgatt accggctggg
      901 tgcaaacaat catctaccgg gagggtgagg gccgtgtaga actcaggttc accaaagaca
      961 tgctgccgta cctgacggaa ctcaccaaac agttcaccaa atacgccttg gctgacgtgg
     1021 ccaagatgga cagcacccac gcgatcaggc tttacgagct gctcatgcaa tgggacagca
     1081 tcggccagcg cgaaatagaa attgaccagc tgcgaaagtg gtttcaactg gaaggccggt
     1141 atccctcgat caaggacttc aagttgcgag tgcttgatcc agccgtgacg cagatcaacg
     1201 agcacagccc gctacaggtg gagtgggcgc agcgaaagac cgggcgcaag gtcacacatc
     1261 tgttgttcag ttttggaccg aagaagcccg ccaaggcggt gggtaaggcc ccagcgaagc
     1321 gcaaggccgg gaagatttca gatgctgaga tcgcgaaaca ggctcgccct ggtgagacat
     1381 gggaagcggc ccgcgctcga ctaacccaga tgccgctgga tctggcctag aggccgtggc
     1441 caccacggcc cggcctgcct ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1501 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1561 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat
     1621 gcaggcctct gcagtcgacg ggcccgggat ccgatagtac tagctgttga ctcgcttggg
     1681 attttcggaa tatcatgccg ggtctgacaa tcggatcgag cttcgaaagg acaagcatat
     1741 ggacctcgat cagatcgtgg cggatgccca gcagagcttt gagggcgcgg ccgacattac
     1801 gaccttggaa aatgaaaagg cccgcttcct gggcaagagc ggtgccctca cggaactctt
     1861 gaaggggctg ggtaagctgg acccggaaac ccggaagacc gagggtgccc ggattaacgt
     1921 ggccaagcag caggtggagg cggccttgaa tgcccggcgg caggccttgg cggatgccct
     1981 tctcaatcag cgcctggcgg ctgaagccat tgatgtgacc ctccccggtc gcggggcggg
     2041 cgccggctcg ctgcaccccg tcatgcgaac ctgggaacgt gtggagcaga ttttccgtag
     2101 cattggcttc gatgtcgcgg atggaccgga aattgagacg gattggtata atttcacggc
     2161 gctcaactcg cccgaaaacc accccgcacg gtccatgcag gacaccttct atgtggatgg
     2221 taaggacgcc gatgggcggc ccctcctcct ccggacccat acctcgccca tgcaggtgcg
     2281 gtacgcccgc atgaatcgac ccccgattaa ggtgattgct cccggtcgga cctatcgggt
     2341 cgattcggac gccacccata gccccatgtt taatcaggtg gaggggctct ggattgatga
     2401 aaacgtgtcg tttgccgatc tcaagggtgc ctacaccgac tttctcaaga aatttttcga
     2461 acgagatgac atcttggtcc gtttccgccc gagctacttc cccttcacgg aacccagcgc
     2521 ggaaattgat atgatgtttg aacatgggaa gaatgctggt aagtggttgg aaatcagcgg
     2581 cagcggtcag gtccacccca cggtcattcg gaatatgggc ctcgacccgg agcgttacat
     2641 tggcttcggc ttcggttccg ggttggaacg ccttaccatg ctccggtatg gtgtccagga
     2701 cctccggctc tttttcgaaa atgacctccg gttcctccgc cagttcgcct aaagatcttt
     2761 catctagagg atccccctag agaataggaa cttcggaata ggaacttcaa agcgtttccg
     2821 aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc
     2881 gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt
     2941 gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt
     3001 acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc
     3061 tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc
     3121 atttcctttg atattggatc atatgcatag taccgagaaa ctagtgcgaa gtagtgatca
     3181 ggtattgctg ttatctgatg agtatacgtt gtcctggcca cggcagaagc acgcttatcg
     3241 ctccaatttc ccacaacatt agtcaactcc gttaggccct tcattgaaag aaatgaggtc
     3301 atcaaatgtc ttccaatgtg agattttggg ccatttttta tagcaaagat tgaataaggc
     3361 gcatttttct tcaaagcttt attgtacgat ctgactaagt tatcttttaa taattggtat
     3421 tcctgtttat tgcttgaaga attgccggtc ctatttactc gttttaggac tggttcagaa
     3481 ttcctcaaaa attcatccaa atatacaagt ggatcgatcc taccccttgc gctaaagaag
     3541 tatatgtgcc tactaacgct tgtctttgtc tctgtcacta aacactggat tattactccc
     3601 agatacttat tttggactaa tttaaatgat ttcggatcaa cgttcttaat atcgctgaat
     3661 cttccacaat tgatgaaagt agctaggaag aggaattggt ataaagtttt tgtttttgta
     3721 aatctcgaag tatactcaaa cgaatttagt attttctcag tgatctccca gatgctttca
     3781 ccctcactta gaagtgcttt aagcattttt ttactgtggc tatttccctt atctgcttct
     3841 tccgatgatt cgaactgtaa ttgcaaacta cttacaatat cagtgatatc agattgatgt
     3901 ttttgtccat agtaaggaat aattgtaaat tcccaagcag gaatcaattt ctttaatgag
     3961 gcttccagaa ttgttgcttt ttgcgtcttg tatttaaact ggagtgattt attgacaata
     4021 tcgaaactca gcgaattgct tatgatagta ttatagctca tgaatgtggc tctcttgatt
     4081 gctgttccgt tatgtgtaat catccaacat aaataggtta gttcagcagc acataatgct
     4141 attttctcac ctgaaggtct ttcaaacctt tccacaaact gacgaacaag caccttaggt
     4201 ggtgttttac ataatatacc aaattgtggc atacaacctc cttagtacat gcaaccatta
     4261 tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttatcc cttgcggtga
     4321 tagatttaac gtatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca
     4381 cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct
     4441 gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat
     4501 gcattaaatg cttataacgc cgcattgctt acaaaaattc tcaaagttag cgttgaagaa
     4561 tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg
     4621 tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca
     4681 cctaagctta gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa
     4741 gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc
     4801 tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag
     4861 ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc
     4921 agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc
     4981 aatgagagtt gttccgttgt ggggaaagtt atcgctagtc agtggcctga agagacgttt
     5041 ggctgatcgg caaggtgttc tggtcggcgc atagctgata acaattgagc aagaatcttc
     5101 atcgaattag gggaattttc actcccctca gaacataaca tagtaaatgg attgaattat
     5161 gaagaatggt ttttatgcga cttaccgcag caaaaataaa gggaaagata agcgctcaat
     5221 aaacctgtct gttttcctta attctctgct ggctgataat catcacctgc aggttggctc
     5281 caattatttg tatattcata aaatcggggt accgagctcg aattcgtaat catggtcata
     5341 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     5401 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     5461 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     5521 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     5581 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     5641 gttatccaca gaatcagggg ataacgcagg aaagaacatg gggattcctt aaggtatact
     5701 ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt
     5761 tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca
     5821 acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt
     5881 cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc
     5941 gataagctag cttatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6001 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6061 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6121 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6181 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6241 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6301 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6361 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6421 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     6481 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6541 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6601 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6661 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6721 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6781 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6841 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6901 aatgataccg cctcctcgaa tcagcgctcc agcaggatgc gcaacatgcg ccgcagcggc
     6961 tcggcagcgc cccacagcaa ctggtcgccg acggtgaagg cgccgagata ctgggagacc
     7021 atgttcaact tgcgcaggcg cccgaccgga acgctcaggg tgccggtgac ggcggccggg
     7081 gtcagctcgc gcacgcttac ctcgcggtgg ttcggcacca gcttgaccca gggattgtgc
     7141 tgtctgatca ggccctcgat gtcggtcagc ggcacatcct tgttcaactt gatggtcagc
     7201 gcctggctgt ggcaacgcat ggcgccgacg cgcacgcaga taccatcgac cgggatcggg
     7261 ttcttgaagc gggcaaggat cttgttggtc tccgcctggg ccttccactc ttcgcggctc
     7321 tgtctccgtt gggacagttc cttgtcgatc caggggatca ggctgccgcc cagcggcgcg
     7381 ccgaaatgtt cggtggggaa ggcttcgctg cgcagggtct ccgccacctt gcggtcgatg
     7441 tcgaggatcg cgctggccgg gttggccagg tcatccgcca ccgatgcatg ggcggcgccc
     7501 atctgcttga gcagatcacg catgttctgc gcgccggcgc cggaggcggc ctggtaggtc
     7561 atggcactca tccactcgac cagaccggcg tcgaacagcc cgcccagggc catcagcatc
     7621 aggctgacgg tgcagttgcc gccgatgtag ttgcgggtgc cggcgtccag agcctggtcg
     7681 atgaccttgc ggttgaccgg gtcgaggacg atcaccgcgt cgtcttccat gcgcagactg
     7741 gaggcggcgt cgatccagta gccctgccag ccggcctcgc gcagcttggg gaatacttcg
     7801 ctggtgtagt cgccgccctg gcaggtcagg atcacgtcga gggtcttcag ctcgtcgatg
     7861 ctgtaggcgt ccttcagcgg agcgatgtcc ttgtccactt ccggggcctg ggcaccgacg
     7921 ttcgacgtgg tgaagaacac cggctcgatc aggtcgaagt cccgctcttc cagcattcgc
     7981 tgtatgagta cggaacccac catcccgcgc cacccgatca gacctacacg cttcatcgca
     8041 actacacctc ataggggatc gcatccgttc attgaacacc tcctttttct cacccggcgc
     8101 agcaggaaag ctgcttcaca tccttgttga aggtctgcgc cgcgagcttc aacccctcga
     8161 ccatcgtcag gtaggggaac aactggtcgg ccagttcctg caccgtcatg cggttgcgaa
     8221 tggccagagc cgccgtctgg atcagttcac ccgcttccgg cgcgaccgcc tgtacgccga
     8281 tcagccgatg gctgccttcc tcgataacca acttgatgaa gccgcgtgtg tcgaagttgg
     8341 cgagcgcacg cggcacgttg tccaaggtca aggtgcggct gtcggtctcg atcccgtcgt
     8401 ggtgggcttc cgcctcgctg tagcccacgg tcgccacttg cggatcggtg aacaccacgg
     8461 ccggcattgc ggtcaggtcg agcgccgcat cgccgccggt catgttgatc gcggcacggg
     8521 tgccggccgc tgccgccaca tagacgaact gcggctggtc ggtgcagtcg ccggccgcgt
     8581 agatgttcgg gttgctcgtg cgcatacctt ggtcgatgac gatggcacct tgcgcattga
     8641 cagtgacccc cgctgcgtcc agcgcgaggc tgcgcgtgtt cggtgtccga ccggtggcaa
     8701 ccagcagttt gtcggcgcgc aattcaccgt gcgtggtggt cagcacgaac tcaccgtcca
     8761 tatgggcgac ctggctggct tgcgtgtgct ccagcacctc gatgccctcg gcacggaaag
     8821 cggctgtcac cgcctcgccg atggccgggt cttcacggaa gaacaaggta ttgcgcgcca
     8881 ggaccgtgac cttgctgccc agccgggcaa aggcttgcgc cagctccagc gccaccaccg
     8941 acgagccgat tacggcaagg cgttcgggaa tggtgtcgct cgccagggcc tcggtggaag
     9001 tccagtaggg tgactctttc aaccccggaa tcggcgggac cgccgggctg gcacccgtgg
     9061 cgaccaggca gcggtcgaac atcacgacgc gctcgccacc ctcgttcaaa cggacggtaa
     9121 ggctctggtc gtccttgaag cgcgcctcac cgtgcacaac ggtgatggcc ggattaccgc
     9181 ccaggatgcc ttcgtacttg gcgtgccgca gttcgtccac gcgggcctgc tgctgggcca
     9241 gcagcttact gcggtcaatc gtaggcacag ttgccgcaat accgccatcg aacgggcttt
     9301 cccggcgcag atgggcgatg tgggcggcgc ggatcatgat cttggacggc acacagccga
     9361 cattgacgca ggtgccgccg atggtgccgc gctcgatcag cgtgacctgc gcgccttgct
     9421 cgacggcctt cagcgccgcc gccatcgcgg ctccaccgct gccaatgacc gctacctgca
     9481 ccgggggctc gttgccactg tgcttttcgg cggcggccat ccatccccgc accttgtcga
     9541 gcagtccgac gcggttgtcc gccagtggcg catcggctag cgttgccttg tagcccagtc
     9601 cggccacggc ggcagtcagc gcgtccggcg atgtgcccgg cacgatggcg agttgcgctg
     9661 tgcccttcgg ataggacacc agcgccgact gcacgcctgg cactttttcc agcgcttcct
     9721 tgacgtgcgc cgcgcacgag tcgcaagtca tgccggtgat ttttagatgg gtcatgcaac
     9781 agatccttta tcgtttgtgg cgccagacaa tgacgtccgt tgtgctgcgg tgccgttttc
     9841 agtgactcac tgcttgacgc tggacggata gcccgcgtct gcggttgcct tggtcagctt
     9901 ctgcacgctg gtcttggcat cgtcgaaggt gacgaccgct tggcgtgtct cgaaagtcac
     9961 gtcaactttg ctgacgcctt cgacctcgga aatcgccttc ttgacagtga tcgggcaggc
    10021 ggagcaggtc atgcccggta cggacagcgt gacggtctgg gtggcggccc agacgggggc
    10081 aacaacggcg gcgagggcga gggaggcaaa cagtttcttc atggtgaact ccgatcagta
    10141 gaaaaatggc atgacgtagg gaaatccgag cgcgaccaga accagcgcgg ccacgatcca
    10201 gaaaatgagc ttgtaagtag ctcgcacttg gggaatcgcg cagacctcac ccggtttgca
    10261 ggccgctgcc tgccggtaga tgcgccgcca ggcgaagaac aacgccacca gcgccacgcc
    10321 gataaagatg gggcgatagg gttccaacac cgccaagttg ccgatccaag cgccgctgaa
    10381 ccccaaggcg atcagaacca acggcccgag gcagcaagcc gaggcgagga tggcggcaag
    10441 ccctccagtg aagagcgcgc cgcgcccggt ttttggttca gacatgcgct tgtcctttcg
    10501 catggtatat ctccttctta aagttaaaca aaattattcg gaacccagca tgatattccg
    10561 gaaataccaa ctaagtcaac ggctgatcat ggtatatctc cttcttaaag attattgaag
    10621 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10681 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    10741 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
