LOCUS       V001286                 7087 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V001286
VERSION     V001286
KEYWORDS    pFlagCMV2-EFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Zou W, Zhang DE
  TITLE     The interferon-inducible ubiquitin-protein isopeptide ligase (E3)
            EFP also functions as an ISG15 E3 ligase.
  JOURNAL   J Biol Chem. 2006 Feb 17. 281(7):3989-94.
   PUBMED   16352599
REFERENCE   2  (bases 1 to 7087)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7087)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol
            Chem. 2006 Feb 17. 281(7):3989-94."
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             618..641
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             645..2534
                     /gene="TRIM25"
                     /label="E3 ubiquitin/ISG15 ligase TRIM25"
                     /note="E3 ubiquitin/ISG15 ligase TRIM25 from Homo sapiens.
                     Accession#: Q14258"
     polyA_signal    3111..3733
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     promoter        3762..4091
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        complement(4130..4148)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4162..4178)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4186..4202)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4210..4240)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4255..4276)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(4393..4410)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4564..5152)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5326..6183)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6184..6288)
                     /label="AmpR promoter"
     rep_origin      6315..6770
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6911..6927
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattgatc taccatggac tacaaagacg atgacgacaa gctcatggca gagctgtgcc
      661 ccctggccga ggagctgtcg tgctccatct gcctggagcc cttcaaggag ccggtcacca
      721 ctccgtgcgg ccacaacttc tgcgggtcgt gcctgaatga gacgtgggca gtccagggct
      781 cgccatacct gtgcccgcag tgccgcgccg tctaccaggc gcgaccgcag ctgcacaaga
      841 acacggtgct gtgcaacgtg gtggagcagt tcctgcaggc cgacctggcc cgggagccac
      901 ccgccgacgt ctggacgccg cccgcccgcg cctctgcacc cagcccgaat gcccaggtgg
      961 cctgcgacca ctgcctgaag gaggccgccg tgaagacgtg cttggtgtgc atggcctcct
     1021 tctgtcagga gcacctgcag ccgcacttcg acagccccgc cttccaggac cacccgctgc
     1081 agccgcccgt tcgcgacctg ttgcgccgca aatgttccca gcacaatcgg ctgcgggaat
     1141 ttttctgccc cgagcacagc gagtgcatct gccacatctg cctggtggag cataagacct
     1201 gctctcccgc gtccctgagc caggccagcg ccgacctgga ggccaccctg aggcacaaac
     1261 taactgtcat gtacagtcag atcaacgggg cgtcgagagc actggatgat gtgagaaaca
     1321 ggcagcagga tgtgcggatg actgcaaaca gaaaggtgga gcagctacaa caagaataca
     1381 cggaaatgaa ggctctcttg gacgcctcag agaccacctc gacaaggaag ataaaggaag
     1441 aggagaagag ggtcaacagc aagtttgaca ccatttatca gattctcctc aagaagaaga
     1501 gtgagatcca gaccttgaag gaggagattg aacagagcct gaccaagagg gatgagttcg
     1561 agtttctgga gaaagcatca aaactgcgag gaatctcaac aaagccagtc tacatccccg
     1621 aggtggaact gaaccacaag ctgataaaag gcatccacca gagcaccata gacctcaaaa
     1681 acgagctgaa gcagtgcatc gggcggctcc aggagctcac ccccagttca ggtgaccctg
     1741 gagagcatga cccagcgtcc acacacaaat ccacacgccc tgtgaagaag gtctccaaag
     1801 aggaaaagaa atccaagaaa cctccccctg tccctgcctt acccagcaag cttcccacgt
     1861 ttggagcccc ggaacagtta gtggatttaa aacaagctgg cttggaggct gcagccaaag
     1921 ccaccagctc acatccgaac tcaacatctc tcaaggccaa ggtgctggag accttcctgg
     1981 ccaagtccag acctgagctc ctggagtatt acattaaagt catcctggac tacaacaccg
     2041 cccacaacaa agtggctctg tcagagtgct atacagtagc ttctgtggct gagatgcctc
     2101 agaactaccg gccgcatccc cagaggttca catactgctc tcaggtgctg ggcctgcact
     2161 gctacaagaa ggggatccac tactgggagg tggagctgca gaagaacaac ttctgtgggg
     2221 taggcatctg ctacggaagc atgaaccggc agggcccaga aagcaggctc ggccgcaaca
     2281 gcgcctcctg gtgcgtggag tggttcaaca ccaagatctc tgcctggcac aataacgtgg
     2341 agaaaaccct gccctccacc aaggccacgc gggtgggcgt gcttctcaac tgtgaccacg
     2401 gctttgtcat cttcttcgct gttgccgaca aggtccacct gatgtataag ttcagggtgg
     2461 actttactga ggctttgtac ccggctttct gggtattttc tgctggtgcc acactctcca
     2521 tctgctcccc caagtaggca ggctgtaggc acttgggctg actgcctgca gaagtcccaa
     2581 gaccctagtg aaaatacagc aggcagaact ctccttggat aattccccaa gaggtcccaa
     2641 ggattgggag catgggaggg gagctggcgg gagggtggga ggtgggattt agccaggaaa
     2701 ggggtgagag tgattgtgtt gtgggcgagg aggcgtttcc accccctggt gcctatcagg
     2761 gcagggtgac ctactcccca ttgttctgga aatctccagg ctgctgggca gctgggcaga
     2821 gctctgggaa gtgaagtcat gagtgcccga ttcctcttag agaaaatcca tagctactgt
     2881 aggttctgtc ttgggccact tggatctgaa ggctgcccct ttgctctctg gggtagcctt
     2941 cagatcttgg tgttttgaat tcttactata gatgttttta aagttccaaa gtcattgagt
     3001 ttcaatgtta cataaactcc attacccgcn aaaaaaaaaa aaaaaaaaaa gggcggccgc
     3061 gaattcatcg atagatctga tatcggtacc agtcgactct agaggatccc gggtggcatc
     3121 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca
     3181 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata
     3241 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg
     3301 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa
     3361 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc
     3421 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca
     3481 tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca
     3541 aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg attttaaaat
     3601 aactatacca gcaggaggac gtccagacac agcataggct acctggccat gcccaaccgg
     3661 tgggacattt gagttgcttg cttggcactg tcctctcatg cgttgggtcc actcagtaga
     3721 tgcctgttga attgggtacg cggccagctt ggctgtggaa tgtgtgtcag ttagggtgtg
     3781 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3841 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3901 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3961 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4021 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4081 gcttttgcaa aaagctcctc gaggaactga aaaaccagaa agttaattcc ctatagtgag
     4141 tcgtattaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     4201 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4261 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4321 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4501 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4561 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4681 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4741 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4801 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4861 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4921 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4981 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5041 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5101 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6061 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6301 aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     6361 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     6421 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     6481 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     6541 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     6601 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     6661 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     6721 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt ccattcgcca
     6781 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     6841 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     6901 tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa tcaatattgg
     6961 caattagcca tattagtcat tggttatata gcataaatca atattggcta ttggccattg
     7021 catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg
     7081 ccatgtt
//
