LOCUS       V006237                 6031 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006237
VERSION     V006237
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6031)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6031)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     141..157
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     enhancer        318..697
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             935..958
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             965..2113
                     /gene="Mapk8"
                     /label="Mitogen-activated protein kinase 8"
                     /note="Mitogen-activated protein kinase 8 from Mus
                     musculus. Accession#: Q91Y86"
     misc_feature    2117..2361
                     /label="3' UTR of hMAPK8"
                     /note="3' UTR of hMAPK8"
     polyA_signal    2372..2994
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     promoter        3023..3352
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        complement(3391..3409)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3423..3439)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3447..3463)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3471..3501)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3516..3537)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3825..4413)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4587..5444)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5445..5549)
                     /label="AmpR promoter"
     rep_origin      5576..6031
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct
      961 tnnnagcaga agcaagcgtg acaacaattt ttatagtgta gagattggag attctacatt
     1021 cacagtcctg aaacgatatc agaatttaaa acctataggc tcaggagctc aaggaatagt
     1081 atgcgcagct tatgatgcca ttcttgaaag aaatgttgca atcaagaagc taagccgacc
     1141 atttcagaat cagactcatg ccaagcgggc ctacagagag ctagttctta tgaaatgtgt
     1201 taatcacaaa aatataattg gccttttgaa tgttttcaca ccacagaaat ccctagaaga
     1261 atttcaagat gtttacatag tcatggagct catggatgca aatctttgcc aagtgattca
     1321 gatggagcta gatcatgaaa gaatgtccta ccttctctat cagatgctgt gtggaatcaa
     1381 gcaccttcat tctgctggaa ttattcatcg ggacttaaag cccagtaata tagtagtaaa
     1441 atctgattgc actttgaaga ttcttgactt cggtctggcc aggactgcag gaacgagttt
     1501 tatgatgacg ccttatgtag tgactcgcta ctacagagca cccgaggtca tccttggcat
     1561 gggctacaag gaaaacgtgg atttatggtc tgtggggtgc attatgggag aaatggtttg
     1621 ccacaaaatc ctctttccag gaagggacta tattgatcag tggaataaag ttattgaaca
     1681 gcttggaaca ccatgtcctg aattcatgaa gaaactgcaa ccaacagtaa ggacttacgt
     1741 tgaaaacaga cctaaatatg ctggatatag ctttgagaaa ctcttccctg atgtcctttt
     1801 cccagctgac tcagaacaca acaaacttaa agccagtcag gcaagggatt tgttatccaa
     1861 aatgctggta atagatgcat ctaaaaggat ctctgtagat gaagctctcc aacacccgta
     1921 catcaatgtc tggtatgatc cttctgaagc agaagctcca ccaccaaaga tccctgacaa
     1981 gcagttagat gaaagggaac acacaataga agagtggaaa gaattgatat ataaggaagt
     2041 tatggacttg gaggagagaa ccaagaatgg agttatacgg gggcagccct ctcctttagc
     2101 acaggtgcag cagtgatcaa tggctctcag catccatcat catcgtcgtc tgtcaatgat
     2161 gtgtcttcaa tgtcaacaga tccgactttg gcctctgata cagacagcag tctagaagca
     2221 gcagctgggc ctctgggctg ctgtagatga ctacttgggc catcgggggg tgggagggat
     2281 ggggagtcgg ttagtcattg atagaactac tttgaaaaca attcagtggt cttatttttg
     2341 ggtgattttt caaaaaatgt annnggatcc cgggtggcat ccctgtgacc cctccccagt
     2401 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     2461 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     2521 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     2581 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     2641 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     2701 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
     2761 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
     2821 gtgaaccact gctcccttcc ctgtccttct gattttaaaa taactatacc agcaggagga
     2881 cgtccagaca cagcataggc tacctggcca tgcccaaccg gtgggacatt tgagttgctt
     2941 gcttggcact gtcctctcat gcgttgggtc cactcagtag atgcctgttg aattgggtac
     3001 gcggccagct tggctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3061 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3121 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3181 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3241 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3301 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctcct
     3361 cgaggaactg aaaaaccaga aagttaattc cctatagtga gtcgtattaa attcgtaatc
     3421 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     3481 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     3541 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     3601 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     3661 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3721 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3781 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3841 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3901 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3961 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4021 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4081 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4141 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4201 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4261 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4321 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4381 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4441 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4501 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4561 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4621 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4681 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4741 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4801 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4861 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4921 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4981 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5041 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5101 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5161 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5221 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5281 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5341 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5401 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5461 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5521 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     5581 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5641 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5701 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5761 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5821 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5881 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5941 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6001 aattttaaca aaatattaac gcttacaatt t
//
