LOCUS       62056_10795        6589 bp DNA     circular SYN 10-NOV-2021
DEFINITION  Synthetic construct DNA, expression vector pFastBacMam6, complete 
            sequence.
ACCESSION   LC658353
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Nakanishi H, Suzuki H, Abe K.
  TITLE     Vectors for baculovirus gene transfer into Mammalian cells to 
            product recombinant 3C_His8_Stop fusion proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6589)
  AUTHORS   Nakanishi H, Suzuki H, Abe K.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2021) Contact:Hanayo Nakanishi Nagoya University, 
            Cellular and Structural Physiology Institute; Furo-cho, Chikusa-ku, 
            Nagoya, Aichi 4648601, Japan
REFERENCE   3  (bases 1 to 6589)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-NOV-2021) Contact:Hanayo Nakanishi Nagoya University, Cellular 
            and Structural Physiology Institute; Furo-cho, Chikusa-ku, Nagoya, 
            Aichi 4648601, Japan"
FEATURES             Location/Qualifiers
     source          1..6589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  183..348
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    complement(377..511)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     536..557
                     /label=pFastBac primer R1
                     /note="pFastBac primer R1"
     enhancer        752..1055
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1056..1259
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     1256..1280
                     /label=LNCX_primer
                     /note="LNCX_primer"
     intron          1395..1527
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1572..1590
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1644..1667
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     misc_feature    1668..1712
                     /label=GAAEDQVDPRLIDGK
                     /note="GAAEDQVDPRLIDGK"
     CDS             1713..1736
                     /label=8xHis
                     /note="8xHis affinity tag"
     misc_feature    1740..2328
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    complement(2340..2461)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(2595..2686)
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        3038..3066
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3255..3785
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     mobile_element  3855..4079
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(4382..4970)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5144..6001)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6002..6106)
                     /label=AmpR promoter
     rep_origin      complement(6133..6588)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ccattcgcca ttcaggctgc aaataagcgt tgatattcag tcaattacaa acattaataa
       61 cgaagagatg acagaaaaat tttcattctg tgacagagaa aaagtagccg aagatgacgg
      121 tttgtcacat ggagttggca ggatgtttga ttaaaaacat aacaggaaga aaaatgcccc
      181 gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg aaatggagtt tttaaggatt
      241 atttagggaa gagtgacaaa atagatggga actgggtgta gcgtcgtaag ctaatacgaa
      301 aattaaaaat gacaaaatag tttggaacta gatttcactt atctggttcg gatctcctag
      361 gctcaagcag tgatcagatc cagacatgat aagatacatt gatgagtttg gacaaaccac
      421 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
      481 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
      541 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
      601 tatggctgat tatgatcctc tagtacttct cgacaagctt ggtaccgcat gcctcgagac
      661 tgcaggctct agattcgaaa gcggccgcga ctagttatta atagtaatca attacggggt
      721 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      781 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      841 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      901 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg
      961 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc
     1021 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca
     1081 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     1141 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg
     1201 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     1261 gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac agttaaattg
     1321 ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcaga agttggtcgt
     1381 gaggcactgg gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac
     1441 tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg
     1501 acatccactt tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag
     1561 gctagagtac ttaatacgac tcactatagg ctagcctcga gaattcacgc gtggtacctc
     1621 tagagtcgac ccgggcggcc gcattggaag ttctgtttca agggcctggg gccgcagaag
     1681 atcaagttga tccaagattg attgatggca agcatcacca tcaccatcac catcactaga
     1741 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     1801 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     1861 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     1921 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     1981 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     2041 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     2101 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     2161 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     2221 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     2281 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gagacatgat
     2341 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2401 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2461 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     2521 ttaaagcaag taaaacctct acaaatgtgg taggatccgc gcccgatggt gggacggtat
     2581 gaataatccg gaatatttat aggttttttt attacaaaac tgttacgaaa acagtaaaat
     2641 acttatttat ttgcgagatg gttatcattt taattatctc catgatctat taatattccg
     2701 gagtatacgt agccaaccac tagaactata gctagagtcc tgggcgaaca aacgatgctc
     2761 gccttccaga aaaccgagga tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg
     2821 ccgcgacggc cgaggtcttc cgatctcctg aagccagggc agatccgtgc acagcacctt
     2881 gccgtagaag aacagcaagg ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg
     2941 ctcgttcgcc agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg
     3001 acgcacaccg tggaaacgga tgaaggcacg aacccagttg acataagcct gttcggttcg
     3061 taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     3121 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca
     3181 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     3241 atggagcagc aacgatgtta cgcagcagca acgatgttac gcagcagggc agtcgcccta
     3301 aaacaaagtt aggtggctca agtatgggca tcattcgcac atgtaggctc ggccctgacc
     3361 aagtcaaatc catgcgggct gctcttgatc ttttcggtcg tgagttcgga gacgtagcca
     3421 cctactccca acatcagccg gactccgatt acctcgggaa cttgctccgt agtaagacat
     3481 tcatcgcgct tgctgccttc gaccaagaag cggttgttgg cgctctcgcg gcttacgttc
     3541 tgcccaggtt tgagcagccg cgtagtgaga tctatatcta tgatctcgca gtctccggcg
     3601 agcaccggag gcagggcatt gccaccgcgc tcatcaatct cctcaagcat gaggccaacg
     3661 cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg tgacgatccc gcagtggctc
     3721 tctatacaaa gttgggcata cgggaagaag tgatgcactt tgatatcgac ccaagtaccg
     3781 ccacctaaca attcgttcaa gccgagatcg gcttcccggc cgcggagttg ttcggtaaat
     3841 tgtcacaacg ccgcgaatat agtctttacc atgcccttgg ccacgcccct ctttaatacg
     3901 acgggcaatt tgcacttcag aaaatgaaga gtttgcttta gccataacaa aagtccagta
     3961 tgctttttca cagcataact ggactgattt cagtttacaa ctattctgtc tagtttaaga
     4021 ctttattgtc atagtttaga tctattttgt tcagtttaag actttattgt ccgcccacac
     4081 ccgcttacgc agggcatcca tttattactc aaccgtaacc gattttgcca ggttacgcgg
     4141 ctggtctgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     4201 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4261 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4321 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4381 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4441 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4501 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4561 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4621 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4681 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4741 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4801 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     4861 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4921 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4981 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5041 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     5101 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     5161 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     5221 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     5281 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     5341 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     5401 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     5461 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     5521 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     5581 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     5641 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     5701 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     5761 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     5821 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     5881 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     5941 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     6001 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     6061 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     6121 aagtgccacc tgaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt
     6181 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     6241 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     6301 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     6361 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     6421 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     6481 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     6541 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtc
//
