LOCUS       62056_10790        7446 bp DNA     circular SYN 10-NOV-2021
DEFINITION  Synthetic construct DNA, expression vector pFastBacMam5, complete 
            sequence.
ACCESSION   LC658352
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Nakanishi H, Suzuki H, Abe K.
  TITLE     Vectors for baculovirus gene transfer into Mammalian cells to 
            product recombinant Flag-His8-eGFP-3C fusion proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7446)
  AUTHORS   Nakanishi H, Suzuki H, Abe K.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2021) Contact:Hanayo Nakanishi Nagoya University, 
            Cellular and Structural Physiology Institute; Furo-cho, Chikusa-ku, 
            Nagoya, Aichi 4648601, Japan
REFERENCE   3  (bases 1 to 7446)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-NOV-2021) Contact:Hanayo Nakanishi Nagoya University, Cellular 
            and Structural Physiology Institute; Furo-cho, Chikusa-ku, Nagoya, 
            Aichi 4648601, Japan"
FEATURES             Location/Qualifiers
     source          1..7446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  183..348
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    complement(377..511)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     536..557
                     /label=pFastBac primer R1
                     /note="pFastBac primer R1"
     enhancer        797..1175
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1176..1379
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     1376..1400
                     /label=LNCX_primer
                     /note="LNCX_primer"
     intron          1515..1647
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1692..1710
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1730..1753
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1775..1798
                     /label=8xHis
                     /note="8xHis affinity tag"
     CDS             1799..2515
                     /label=mEGFP
                     /note="enhanced GFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
     CDS             2525..2548
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     misc_feature    2594..2596
                     /label=stop codon
                     /note="stop codon"
     misc_feature    2597..3185
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    complement(3197..3318)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3452..3543)
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        3895..3923
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             4112..4642
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     mobile_element  4712..4936
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(5239..5827)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6001..6858)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6859..6963)
                     /label=AmpR promoter
     rep_origin      complement(6990..7445)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ccattcgcca ttcaggctgc aaataagcgt tgatattcag tcaattacaa acattaataa
       61 cgaagagatg acagaaaaat tttcattctg tgacagagaa aaagtagccg aagatgacgg
      121 tttgtcacat ggagttggca ggatgtttga ttaaaaacat aacaggaaga aaaatgcccc
      181 gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg aaatggagtt tttaaggatt
      241 atttagggaa gagtgacaaa atagatggga actgggtgta gcgtcgtaag ctaatacgaa
      301 aattaaaaat gacaaaatag tttggaacta gatttcactt atctggttcg gatctcctag
      361 gctcaagcag tgatcagatc cagacatgat aagatacatt gatgagtttg gacaaaccac
      421 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
      481 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
      541 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
      601 tatggctgat tatgatcctc tagtacttct cgacaagctt ggtaccccta ggagatcttc
      661 aatattggcc attagccata ttattcattg gttatatagc ataaatcaat attggctatt
      721 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
      781 tatgaccgcc atgttggcat tgattattga ctagttatta atagtaatca attacggggt
      841 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      901 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      961 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     1021 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg
     1081 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc
     1141 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca
     1201 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     1261 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg
     1321 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     1381 gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac agttaaattg
     1441 ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcaga agttggtcgt
     1501 gaggcactgg gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac
     1561 tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg
     1621 acatccactt tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag
     1681 gctagagtac ttaatacgac tcactatagg gatcgccggc accatgggtg actacaagga
     1741 cgacgacgac aagagcagcg gcatgggcag cagccatcat catcatcatc accaccacat
     1801 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1861 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1921 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1981 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     2041 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     2101 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2161 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2221 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2281 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2341 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2401 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2461 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagagcag
     2521 cggcttggaa gttctgtttc aagggccagc tagcgaattc gtcgactcta gactcgaggc
     2581 atgcgcggcc gcatagaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     2641 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     2701 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     2761 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     2821 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     2881 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     2941 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     3001 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     3061 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     3121 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     3181 cccgcctgag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     3241 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     3301 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     3361 gagatgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtag gatccgcgcc
     3421 cgatggtggg acggtatgaa taatccggaa tatttatagg tttttttatt acaaaactgt
     3481 tacgaaaaca gtaaaatact tatttatttg cgagatggtt atcattttaa ttatctccat
     3541 gatctattaa tattccggag tatacgtagc caaccactag aactatagct agagtcctgg
     3601 gcgaacaaac gatgctcgcc ttccagaaaa ccgaggatgc gaaccacttc atccggggtc
     3661 agcaccaccg gcaagcgccg cgacggccga ggtcttccga tctcctgaag ccagggcaga
     3721 tccgtgcaca gcaccttgcc gtagaagaac agcaaggccg ccaatgcctg acgatgcgtg
     3781 gagaccgaaa ccttgcgctc gttcgccagc caggacagaa atgcctcgac ttcgctgctg
     3841 cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagttgaca
     3901 taagcctgtt cggttcgtaa actgtaatgc aagtagcgta tgcgctcacg caactggtcc
     3961 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     4021 gactgttttt ttgtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     4081 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagcaacg atgttacgca
     4141 gcagggcagt cgccctaaaa caaagttagg tggctcaagt atgggcatca ttcgcacatg
     4201 taggctcggc cctgaccaag tcaaatccat gcgggctgct cttgatcttt tcggtcgtga
     4261 gttcggagac gtagccacct actcccaaca tcagccggac tccgattacc tcgggaactt
     4321 gctccgtagt aagacattca tcgcgcttgc tgccttcgac caagaagcgg ttgttggcgc
     4381 tctcgcggct tacgttctgc ccaggtttga gcagccgcgt agtgagatct atatctatga
     4441 tctcgcagtc tccggcgagc accggaggca gggcattgcc accgcgctca tcaatctcct
     4501 caagcatgag gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag attacggtga
     4561 cgatcccgca gtggctctct atacaaagtt gggcatacgg gaagaagtga tgcactttga
     4621 tatcgaccca agtaccgcca cctaacaatt cgttcaagcc gagatcggct tcccggccgc
     4681 ggagttgttc ggtaaattgt cacaacgccg cgaatatagt ctttaccatg cccttggcca
     4741 cgcccctctt taatacgacg ggcaatttgc acttcagaaa atgaagagtt tgctttagcc
     4801 ataacaaaag tccagtatgc tttttcacag cataactgga ctgatttcag tttacaacta
     4861 ttctgtctag tttaagactt tattgtcata gtttagatct attttgttca gtttaagact
     4921 ttattgtccg cccacacccg cttacgcagg gcatccattt attactcaac cgtaaccgat
     4981 tttgccaggt tacgcggctg gtctgcggtg tgaaataccg cacagatgcg taaggagaaa
     5041 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5101 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5161 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5221 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5281 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5341 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5401 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     5461 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5521 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5581 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5641 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     5701 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5761 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5821 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5881 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     5941 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     6001 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     6061 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     6121 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     6181 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     6241 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     6301 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     6361 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     6421 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     6481 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     6541 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     6601 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     6661 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     6721 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     6781 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     6841 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     6901 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     6961 gcgcacattt ccccgaaaag tgccacctga aattgtaaac gttaatattt tgttaaaatt
     7021 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     7081 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     7141 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     7201 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     7261 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     7321 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     7381 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     7441 cgcgtc
//
