LOCUS       62056_10775        7404 bp DNA     circular SYN 10-NOV-2021
DEFINITION  Synthetic construct DNA, expression vector pFastBacMam2, complete 
            sequence.
ACCESSION   LC658349
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Nakanishi H, Suzuki H, Abe K.
  TITLE     Vectors for baculovirus gene transfer into Mammalian cells to 
            product recombinant His8-eGFP-TEV fusion proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Nakanishi H, Suzuki H, Abe K.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2021) Contact:Hanayo Nakanishi Nagoya University, 
            Cellular and Structural Physiology Institute; Furo-cho, Chikusa-ku, 
            Nagoya, Aichi 4648601, Japan
REFERENCE   3  (bases 1 to 7404)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-NOV-2021) Contact:Hanayo Nakanishi Nagoya University, Cellular 
            and Structural Physiology Institute; Furo-cho, Chikusa-ku, Nagoya, 
            Aichi 4648601, Japan"
FEATURES             Location/Qualifiers
     source          1..7404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  183..348
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    complement(377..511)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        797..1175
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1176..1379
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          1515..1647
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1692..1710
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1730..1753
                     /label=8xHis
                     /note="8xHis affinity tag"
     CDS             1754..2470
                     /label=mEGFP
                     /note="enhanced GFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
     CDS             2480..2500
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    2552..2554
                     /label=stop codon
                     /note="stop codon"
     misc_feature    2555..3143
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    complement(3155..3276)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3410..3501)
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        3853..3881
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             4070..4600
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     mobile_element  4670..4894
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(5197..5785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5959..6816)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6817..6921)
                     /label=AmpR promoter
     rep_origin      complement(6948..7403)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ccattcgcca ttcaggctgc aaataagcgt tgatattcag tcaattacaa acattaataa
       61 cgaagagatg acagaaaaat tttcattctg tgacagagaa aaagtagccg aagatgacgg
      121 tttgtcacat ggagttggca ggatgtttga ttaaaaacat aacaggaaga aaaatgcccc
      181 gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg aaatggagtt tttaaggatt
      241 atttagggaa gagtgacaaa atagatggga actgggtgta gcgtcgtaag ctaatacgaa
      301 aattaaaaat gacaaaatag tttggaacta gatttcactt atctggttcg gatctcctag
      361 gctcaagcag tgatcagatc cagacatgat aagatacatt gatgagtttg gacaaaccac
      421 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
      481 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
      541 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
      601 tatggctgat tatgatcctc tagtacttct cgacaagctt ggtaccccta ggagatcttc
      661 aatattggcc attagccata ttattcattg gttatatagc ataaatcaat attggctatt
      721 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
      781 tatgaccgcc atgttggcat tgattattga ctagttatta atagtaatca attacggggt
      841 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      901 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      961 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     1021 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg
     1081 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc
     1141 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca
     1201 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     1261 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg
     1321 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     1381 gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac agttaaattg
     1441 ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcaga agttggtcgt
     1501 gaggcactgg gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac
     1561 tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg
     1621 acatccactt tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag
     1681 gctagagtac ttaatacgac tcactatagg gatcgccatg ggcagcagcc atcatcatca
     1741 tcatcaccac cacatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     1801 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     1861 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     1921 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     1981 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2041 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     2101 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2161 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     2221 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     2281 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2341 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
     2401 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     2461 gctgtacaag agcagcggcg agaacctgta cttccaggga ggtggagcta gcgaattcgt
     2521 cgactctaga ctcgaggcat gcgcggccgc atagaatcaa cctctggatt acaaaatttg
     2581 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     2641 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     2701 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2761 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     2821 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     2881 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2941 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     3001 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     3061 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     3121 ctccctttgg gccgcctccc cgcctgagac atgataagat acattgatga gtttggacaa
     3181 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     3241 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     3301 atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     3361 tgtggtagga tccgcgcccg atggtgggac ggtatgaata atccggaata tttataggtt
     3421 tttttattac aaaactgtta cgaaaacagt aaaatactta tttatttgcg agatggttat
     3481 cattttaatt atctccatga tctattaata ttccggagta tacgtagcca accactagaa
     3541 ctatagctag agtcctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga
     3601 accacttcat ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc
     3661 tcctgaagcc agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc
     3721 aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat
     3781 gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     3841 gcacgaaccc agttgacata agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg
     3901 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     3961 gttttcatgg cttgttatga ctgttttttt gtacagtcta tgcctcgggc atccaagcag
     4021 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     4081 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaggtg gctcaagtat
     4141 gggcatcatt cgcacatgta ggctcggccc tgaccaagtc aaatccatgc gggctgctct
     4201 tgatcttttc ggtcgtgagt tcggagacgt agccacctac tcccaacatc agccggactc
     4261 cgattacctc gggaacttgc tccgtagtaa gacattcatc gcgcttgctg ccttcgacca
     4321 agaagcggtt gttggcgctc tcgcggctta cgttctgccc aggtttgagc agccgcgtag
     4381 tgagatctat atctatgatc tcgcagtctc cggcgagcac cggaggcagg gcattgccac
     4441 cgcgctcatc aatctcctca agcatgaggc caacgcgctt ggtgcttatg tgatctacgt
     4501 gcaagcagat tacggtgacg atcccgcagt ggctctctat acaaagttgg gcatacggga
     4561 agaagtgatg cactttgata tcgacccaag taccgccacc taacaattcg ttcaagccga
     4621 gatcggcttc ccggccgcgg agttgttcgg taaattgtca caacgccgcg aatatagtct
     4681 ttaccatgcc cttggccacg cccctcttta atacgacggg caatttgcac ttcagaaaat
     4741 gaagagtttg ctttagccat aacaaaagtc cagtatgctt tttcacagca taactggact
     4801 gatttcagtt tacaactatt ctgtctagtt taagacttta ttgtcatagt ttagatctat
     4861 tttgttcagt ttaagacttt attgtccgcc cacacccgct tacgcagggc atccatttat
     4921 tactcaaccg taaccgattt tgccaggtta cgcggctggt ctgcggtgtg aaataccgca
     4981 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     5041 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     5101 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     5161 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     5221 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     5281 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     5341 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     5401 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     5461 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5521 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5581 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     5641 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5701 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5761 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5821 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     5881 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     5941 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6001 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     6061 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6121 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     6181 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     6241 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     6301 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     6361 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     6421 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     6481 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     6541 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     6601 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     6661 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6721 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     6781 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     6841 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     6901 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa ttgtaaacgt
     6961 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     7021 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     7081 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     7141 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     7201 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     7261 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     7321 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     7381 taatgcgccg ctacagggcg cgtc
//
