LOCUS       40924_19741        4939 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Baculovirus expression vector pFastBac1-HM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Katagiri Y, Ingham KC.
  TITLE     Enhanced production of green fluorescent fusion proteins in a 
            baculovirus expression system by addition of secretion signal
  JOURNAL   BioTechniques 33 (1), 24-26 (2002)
  PUBMED    12139250
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Sarafanov A, Saenko E.
  TITLE     High-throughput optimization of protein expression in the 
            baculovirus system based on determination of relative expression 
            efficiency of viral stocks
  JOURNAL   Anal. Biochem. 328 (1), 98-100 (2004)
  PUBMED    15081917
REFERENCE   3  (bases 1 to 4939)
  AUTHORS   Sarafanov AG, Makogonenko EM, Pechik IV, Radtke KP, Khrenov AV, 
            Ananyeva NM, Strickland DK, Saenko EL.
  TITLE     Identification of coagulation factor VIII A2 domain residues forming
            the binding epitope for low-density lipoprotein receptor-related 
            protein
  JOURNAL   Biochemistry 45 (6), 1829-1840 (2006)
  PUBMED    16460029
REFERENCE   4  (bases 1 to 4939)
  AUTHORS   Sarafanov AG, Makogonenko EM, Andersen OM, Mikhailenko IA, Ananyeva 
            NM, Khrenov AV, Shima M, Strickland DK, Saenko EL.
  TITLE     Localization of the low-density lipoprotein receptor-related protein
            regions involved in binding to the A2 domain of coagulation factor 
            VIII
  JOURNAL   Thromb. Haemost. 98 (6), 1170-1181 (2007)
  PUBMED    18064310
REFERENCE   5  (bases 1 to 4939)
  AUTHORS   Sarafanov AG.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2004) Biochemistry, American Red Cross, Holland 
            Laboratory, 15601 Crabbs Branch Way, Rockville, MD 20855, USA
REFERENCE   6  (bases 1 to 4939)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 4939)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2002"; volume: "33"; issue: "1"; pages: 
            "24-26"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Anal. 
            Biochem."; date: "2004"; volume: "328"; issue: "1"; pages: "98-100"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: 
            "Biochemistry"; date: "2006"; volume: "45"; issue: "6"; pages: 
            "1829-1840"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Thromb. 
            Haemost."; date: "2007"; volume: "98"; issue: "6"; pages: 
            "1170-1181"
COMMENT     SGRef: number: 5; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2004) Biochemistry, American Red Cross, Holland Laboratory, 
            15601 Crabbs Branch Way, Rockville, MD 20855, USA"
COMMENT     SGRef: number: 6; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        484..588
                     /label=AmpR promoter
     CDS             589..1446
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1620..2208
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(2511..2735)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2805..3335)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(3524..3552)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     RBS             4063..4074
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     sig_peptide     4081..4143
                     /label=melittin signal sequence
                     /note="signal sequence from honeybee melittin"
     CDS             4156..4173
                     /label=6xHis
                     /note="6xHis affinity tag"
     regulatory      4204..4213
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4208..4239
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             4246..4275
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     polyA_signal    4429..4563
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(4592..4757)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     4021 ccatcgggcg cggatcccgg tccgaagcgc gcggaattca ttaaagagga gaaattaact
     4081 atgaaattct tagtcaacgt tgcccttgtt tttatggtcg tatacatttc ttacatctat
     4141 gcggatcgat ggggacatca ccatcaccat cacggatccg caagcgagct cggtaccccg
     4201 gtcgccacca tgggtcgacg atctagaact agtctcgagc cacccgagca aaaactcatc
     4261 tcagaagagg atctgaatag ctgatgagca tgcggtacca agcttgtcga gaagtactag
     4321 aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     4381 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     4441 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     4501 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     4561 atctgatcac tgcttgagcc taggagatcc gaaccagata agtgaaatct agttccaaac
     4621 tattttgtca tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt
     4681 ttgtcactct tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac
     4741 tattttgtcc gcccacagcg gggcattttt cttcctgtta tgtttttaat caaacatcct
     4801 gccaactcca tgtgacaaac cgtcatcttc ggctactttt tctctgtcac agaatgaaaa
     4861 tttttctgtc atctcttcgt tattaatgtt tgtaattgac tgaatatcaa cgcttatttg
     4921 cagcctgaat ggcgaatgg
//
