LOCUS       V014812                 8470 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014812
VERSION     V014812
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8470)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        484..588
                     /label="AmpR promoter"
     CDS             589..1446
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1620..2208
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     mobile_element  complement(2513..2737)
                     /label="Tn7R"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2807..3337)
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        complement(3526..3554)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     promoter        3906..3997
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4049..4066
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             4067..7744
                     /gene="ADAR"
                     /label="Double-stranded RNA-specific adenosine deaminase"
                     /note="Double-stranded RNA-specific adenosine deaminase
                     from Homo sapiens. Accession#: P55265"
     polyA_signal    7960..8094
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(8123..8288)
                     /label="Tn7L"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca gccgcgtaac
     2461 ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg
     2521 gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact
     2581 agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact
     2641 tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa
     2701 gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac aatttaccga
     2761 acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg
     2821 ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg
     2881 cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct
     2941 catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga
     3001 ctgcgagatc atagatatag atctcactac gcggctgctc aaacctgggc agaacgtaag
     3061 ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac
     3121 tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt
     3181 ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc
     3241 cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac
     3301 tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa
     3361 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa
     3421 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     3481 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa
     3541 caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca
     3601 accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt
     3661 tcggtctcca cgcatcgtca ggcattggcg gccttgctgt tcttctacgg caaggtgctg
     3721 tgcacggatc tgccctggct tcaggagatc ggaagacctc ggccgtcgcg gcgcttgccg
     3781 gtggtgctga ccccggatga agtggttcgc atcctcggtt ttctggaagg cgagcatcgt
     3841 ttgttcgccc aggactctag ctatagttct agtggttggc tacgtatact ccggaatatt
     3901 aatagatcat ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg
     3961 ttttcgtaac agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc
     4021 caccatcggg cgcggatccg ccaccatgca tcatcaccat caccatatga atccgcggca
     4081 ggggtattcc ctcagcggat actacaccca tccatttcaa ggctatgagc acagacagct
     4141 cagataccag cagcctgggc caggatcttc ccccagtagt ttcctgctta agcaaataga
     4201 atttctcaag gggcagctcc cagaagcacc ggtgattgga aagcagacac cgtcactgcc
     4261 accttccctc ccaggactcc ggccaaggtt tccagtacta cttgcctcca gtaccagagg
     4321 caggcaagtg gacatcaggg gtgtccccag gggcgtgcat ctcggaagtc aggggctcca
     4381 gagagggttc cagcatcctt caccacgtgg caggagtctg ccacagagag gtgttgattg
     4441 cctttcctca catttccagg aactgagtat ctaccaagat caggaacaaa ggatcttaaa
     4501 gttcctggaa gagcttgggg aagggaaggc caccacagca catgatctgt ctgggaaact
     4561 tgggactccg aagaaagaaa tcaatcgagt tttatactcc ctggcaaaga agggcaagct
     4621 acagaaagag gcaggaacac cccctttgtg gaaaatcgcg gtctccactc aggcttggaa
     4681 ccagcacagc ggagtggtaa gaccagacgg tcatagccaa ggagccccaa actcagaccc
     4741 gagtttggaa ccggaagaca gaaactccac atctgtctca gaagatcttc ttgagccttt
     4801 tattgcagtc tcagctcagg cttggaacca gcacagcgga gtggtaagac cagacagtca
     4861 tagccaagga tccccaaact cagacccagg tttggaacct gaagacagca actccacatc
     4921 tgccttggaa gatcctcttg agtttttaga catggccgag atcaaggaga aaatctgcga
     4981 ctatctcttc aatgtgtctg actcctctgc cctgaatttg gctaaaaata ttggccttac
     5041 caaggcccga gatataaatg ctgtgctaat tgacatggaa aggcaggggg atgtctatag
     5101 acaagggaca acccctccca tatggcattt gacagacaag aagcgagaga ggatgcaaat
     5161 caagagaaat acgaacagtg ttcctgaaac cgctccagct gcaatccctg agaccaaaag
     5221 aaacgcagag ttcctcacct gtaatatacc cacatcaaat gcctcaaata acatggtaac
     5281 cacagaaaaa gtggagaatg ggcaggaacc tgtcataaag ttagaaaaca ggcaagaggc
     5341 cagaccagaa ccagcaagac tgaaaccacc tgttcattac aatggcccct caaaagcagg
     5401 gtatgttgac tttgaaaatg gccagtgggc cacagatgac atcccagatg acttgaatag
     5461 tatccgcgca gcaccaggtg agtttcgagc catcatggag atgccctcct tctacagtca
     5521 tggcttgcca cggtgttcac cctacaagaa actgacagag tgccagctga agaaccccat
     5581 cagcgggctg ttagaatatg cccagttcgc tagtcaaacc tgtgagttca acatgataga
     5641 gcagagtgga ccaccccatg aacctcgatt taaattccag gttgtcatca atggccgaga
     5701 gtttccccca gctgaagctg gaagcaagaa agtggccaag caggatgcag ctatgaaagc
     5761 catgacaatt ctgctagagg aagccaaagc caaggacagt ggaaaatcag aagaatcatc
     5821 ccactattcc acagagaaag aatcagagaa gactgcagag tcccagaccc ccaccccttc
     5881 agccacatcc ttcttttctg ggaagagccc cgtcaccaca ctgcttgagt gtatgcacaa
     5941 attggggaac tcctgcgaat tccgtctcct gtccaaagaa ggccctgccc atgaacccaa
     6001 gttccaatac tgtgttgcag tgggagccca aactttcccc agtgtgagtg ctcccagcaa
     6061 gaaagtggca aagcagatgg ccgcagagga agccatgaag gccctgcatg gggaggcgac
     6121 caactccatg gcttctgata accagcctga aggtatgatc tcagagtcac ttgataactt
     6181 ggaatccatg atgcccaaca aggtcaggaa gattggcgag ctcgtgagat acctgaacac
     6241 caaccctgtg ggtggccttt tggagtacgc ccgctcccat ggctttgctg ctgaattcaa
     6301 gttggtcgac cagtccggac ctcctcacga gcccaagttc gtttaccaag caaaagttgg
     6361 gggtcgctgg ttcccagccg tctgcgcaca cagcaagaag caaggcaagc aggaagcagc
     6421 agatgcggct ctccgtgtct tgattgggga gaacgagaag gcagaacgca tgggtttcac
     6481 agaggtaacc ccagtgacag gggccagtct cagaagaact atgctcctcc tctcaaggtc
     6541 cccagaagca cagccaaaga cactccctct cactggcagc accttccatg accagatagc
     6601 catgctgagc caccggtgct tcaacactct gactaacagc ttccagccct ccttgctcgg
     6661 ccgcaagatt ctggccgcca tcattatgaa aaaagactct gaggacatgg gtgtcgtcgt
     6721 cagcttggga acagggaatc gctgtgtaaa aggagattct ctcagcctaa aaggagaaac
     6781 tgtcaatgac tgccatgcag aaataatctc ccggagaggc ttcatcaggt ttctctacag
     6841 tgagttaatg aaatacaact cccagactgc gaaggatagt atatttgaac ctgctaaggg
     6901 aggagaaaag ctccaaataa aaaagactgt gtcattccat ctgtatatca gcactgctcc
     6961 gtgtggagat ggcgccctct ttgacaagtc ctgcagcgac cgtgctatgg aaagcacaga
     7021 atcccgccac taccctgtct tcgagaatcc caaacaagga aagctccgca ccaaggtgga
     7081 gaacggagaa ggcacaatcc ctgtggaatc cagtgacatt gtgcctacgt gggatggcat
     7141 tcggctcggg gagagactcc gtaccatgtc ctgtagtgac aaaatcctac gctggaacgt
     7201 gctgggcctg caaggggcac tgttgaccca cttcctgcag cccatttatc tcaaatctgt
     7261 cacattgggt taccttttca gccaagggca tctgacccgt gctatttgct gtcgtgtgac
     7321 aagagatggg agtgcatttg aggatggact acgacatccc tttattgtca accaccccaa
     7381 ggttggcaga gtcagcatat atgattccaa aaggcaatcc gggaagacta aggagacaag
     7441 cgtcaactgg tgtctggctg atggctatga cctggagatc ctggacggta ccagaggcac
     7501 tgtggatggg ccacggaatg aattgtcccg ggtctccaaa aagaacattt ttcttctatt
     7561 taagaagctc tgctccttcc gttaccgcag ggatctactg agactctcct atggtgaggc
     7621 caagaaagct gcccgtgact acgagacggc caagaactac ttcaaaaaag gcctgaagga
     7681 tatgggctat gggaactgga ttagcaaacc ccaggaggaa aagaactttt atctctgccc
     7741 agtataggaa ttcaaaggcc tacgtcgacg agctcactag tcgcggccgc tttcgaatct
     7801 agagcctgca gtctcgaggc atgcggtacc aagcttgtcg agaagtacta gaggatcata
     7861 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     7921 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     7981 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8041 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca
     8101 ctgcttgagc ctaggagatc cgaaccagat aagtgaaatc tagttccaaa ctattttgtc
     8161 atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc
     8221 ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc
     8281 cgcccacagc ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc
     8341 atgtgacaaa ccgtcatctt cggctacttt ttctctgtca cagaatgaaa atttttctgt
     8401 catctcttcg ttattaatgt ttgtaattga ctgaatatca acgcttattt gcagcctgaa
     8461 tggcgaatgg
//
