LOCUS       40924_20781        4675 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFYS028, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Scott FY, Ruder WC.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2016) Biological Systems Engineering, Virginia 
            Polytechnic Institute and State University, 1230 Washington Street 
            SW, Blacksburg, VA 24061, USA
REFERENCE   2  (bases 1 to 4675)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4675)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (21-SEP-2016) Biological Systems Engineering, Virginia Polytechnic 
            Institute and State University, 1230 Washington Street SW, 
            Blacksburg, VA 24061, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      21..115
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(139..846)
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     promoter        complement(866..1150)
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     promoter        1241..1525
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     regulatory      1532..1542
                     /regulatory_class="ribosome_binding_site"
     CDS             1990..2535
                     /label=SNAP-tag(R)
                     /note="human O6-alkylguanine-DNA-alkyltransferase variant
                     that forms covalent bonds with benzylguanine derivatives"
     terminator      2635..2721
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2885..3473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(3561..3655)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3688..4545)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4546..4650)
                     /label=AmpR promoter
ORIGIN
        1 gacgtccgag aattggcttg gactcctgtt gatagatcca gtaatgacct cagaactcca
       61 tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag
      121 cagtagtcaa gtactttact tatacagctc gtccatgccg ccggtggagt ggcggccctc
      181 ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccaactt
      241 gatgttgacg ttgtacgcgc cgggcagttg cacgggcttc ttggccttgt aggtggtctt
      301 gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga tctcgccctt
      361 cagcgcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca tagtcttctt
      421 ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa
      481 ctcgccgtct tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt
      541 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt cggggatgtc
      601 ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca ggatgtccca
      661 ggcgaagggc agggggccac ccttggtcac cttcagcttg gcggtctggg tgccctcgta
      721 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat
      781 gtgaaccttg aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct
      841 caccatgttt aagtcctaac tgcagatgga gaaacagtag agagttgcga taaaaagcgt
      901 caggtatgat ccgctaatct tatggataaa aatgctatgg catagcaaag tgtgacgccg
      961 tgcaaataat caatgtggac ttttctgccg tgattataga cacttttgtt acgcgctttt
     1021 gtcatggctt tggtcccgct ttgttacaga atgcttttaa taagcggggt taccggtttg
     1081 gttagcgaga agagccagta aaagacgcag tgacggcaat gtctgatgca atatggacta
     1141 ttggtttctt gtgcaccaag gagcatgcga aggaaacgtt tcgcagaagc ttccgcaagg
     1201 taccactttg ccgcggagta tttgtacatt tgaaggatcc aagaaaccaa tagtccatat
     1261 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1321 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaagcgcgt
     1381 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1441 ctttgctatg ccatagcatt tttatccata agattagcgg atcatacctg acgcttttta
     1501 tcgcaactct ctactgtttc tccatccatg gaggaaggag gagtcgacat gaaagctact
     1561 aaactggtac tgggcgcggt aatcctgggt tctactctgc tggcaggttg ctccagcaac
     1621 gctaaaatcg atcaggaatt caacccgtat gttggctttg aaatgggtta cgactggtta
     1681 ggtcgtatgc cgtacaaagg cagcgttgaa aacggtgcat acaaagctca gggcgttcaa
     1741 ctgaccgcta aactgggtta cccaatcact gacgacctgg acatctacac tcgtctgggt
     1801 ggcatggtat ggcgtgcaga cactaaatcc aacgtttatg gtaaaaacca cgacaccggc
     1861 gtttctccgg tcttcgctgg cggtgttgag tacgcgatca ctcctgaaat cgctacccgt
     1921 ctggaatacc agtggaccaa caacatcggt gacgcacaca ccatcggcac tcgtccggac
     1981 aacgagctca tggacaaaga ttgcgaaatg aaacgtacca ccctggatag cccgctgggc
     2041 aaactggaac tgagcggctg cgaacagggc ctgcatgaaa ttaaactgct gggtaaaggc
     2101 accagcgcgg ccgatgcggt tgaagttccg gccccggccg ccgtgctggg tggtccggaa
     2161 ccgctgatgc aggcgaccgc gtggctgaac gcgtattttc atcagccgga agcgattgaa
     2221 gaatttccgg ttccggcgct gcatcatccg gtgtttcagc aggagagctt tacccgtcag
     2281 gtgctgtgga aactgctgaa agtggttaaa tttggcgaag tgattagcta tcagcagctg
     2341 gcggccctgg cgggtaatcc ggcggccacc gccgccgtta aaaccgcgct gagcggtaac
     2401 ccggtgccga ttctgattcc gtgccatcgt gtggttagct ctagcggtgc ggttggcggt
     2461 tatgaaggtg gtctggcggt gaaagagtgg ctgctggccc atgaaggtca tcgtctgggt
     2521 aaaccgggtc tgggataagc ccgggactac acaattgtcc cccggcgcca gggttgatat
     2581 ctatcgccct agggaccgtc tcgagagaat caatattaat ccaacgcgtg gcatcaaata
     2641 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2701 gctctcctga gtaggacaaa tccgccgccc tagacttagg cgttcggctg cggcgagcgg
     2761 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     2821 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     2881 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2941 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3001 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3061 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3121 gctccaagct gggctgtctg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3181 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3241 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3301 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     3361 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3421 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3481 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3541 tggtcatggc tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
     3601 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     3661 caattcgtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3721 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3781 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     3841 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3901 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3961 ttcgccagtt aatagtttgc gcagcgttgt tgccattgct acaggcatcg tggtgtcacg
     4021 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4081 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4141 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4201 catgccatcc gtaagatgct tttctgtgac tggtgagtat tcaaccaagt cattctgaga
     4261 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4321 acatagcaga actttaaaag tgctcatcat tggaaagcgt tcttcggggc gaaaactctc
     4381 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgcgcac ccaactgatc
     4441 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4501 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4561 gtattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     4621 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacct
//
