LOCUS       40924_20776        3746 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFYS019, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Scott FY, Ruder WC.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2016) Biological Systems Engineering, Virginia 
            Polytechnic Institute and State University, 1230 Washington Street 
            SW, Blacksburg, VA 24061, USA
REFERENCE   2  (bases 1 to 3746)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3746)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (21-SEP-2016) Biological Systems Engineering, Virginia Polytechnic 
            Institute and State University, 1230 Washington Street SW, 
            Blacksburg, VA 24061, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        151..435
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     regulatory      442..452
                     /regulatory_class="ribosome_binding_site"
     CDS             900..1607
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     terminator      1706..1792
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(1956..2544)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(2632..2726)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2759..3616)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3617..3721)
                     /label=AmpR promoter
ORIGIN
        1 gacgtctgtg caagtactac tgttctgcag tcacttgagt tcgataccca gctgggtgga
       61 gtgcaccaag gagcatgcga aggaaacgtt tcgcagaagc ttccgcaagg taccactttg
      121 ccgcggagta tttgtacatt tgaaggatcc aagaaaccaa tagtccatat tgcatcagac
      181 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
      241 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaagcgcgt aacaaaagtg
      301 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
      361 ccatagcatt tttatccata agattagcgg atcatacctg acgcttttta tcgcaactct
      421 ctactgtttc tccatccatg gaggaaggag gagtcgacat gaaagctact aaactggtac
      481 tgggcgcggt aatcctgggt tctactctgc tggcaggttg ctccagcaac gctaaaatcg
      541 atcaggaatt caacccgtat gttggctttg aaatgggtta cgactggtta ggtcgtatgc
      601 cgtacaaagg cagcgttgaa aacggtgcat acaaagctca gggcgttcaa ctgaccgcta
      661 aactgggtta cccaatcact gacgacctgg acatctacac tcgtctgggt ggcatggtat
      721 ggcgtgcaga cactaaatcc aacgtttatg gtaaaaacca cgacaccggc gtttctccgg
      781 tcttcgctgg cggtgttgag tacgcgatca ctcctgaaat cgctacccgt ctggaatacc
      841 agtggaccaa caacatcggt gacgcacaca ccatcggcac tcgtccggac aacgagctca
      901 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
      961 ttcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     1021 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     1081 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     1141 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     1201 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaagacg
     1261 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     1321 tgcagaagaa gactatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     1381 cgctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     1441 aggtcaagac cacctacaag gccaagaagc ccgtgcaact gcccggcgcg tacaacgtca
     1501 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     1561 gcgccgaggg ccgccactcc accggcggca tggacgagct gtataagtaa cccgggacta
     1621 cacaattgtc ccccggcgcc agggttgata tctatcgccc tagggaccgt ctcgagagaa
     1681 tcaatattaa tccaacgcgt ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1741 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc
     1801 ctagacttag gcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1861 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1921 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     1981 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2041 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2101 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2161 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtct gcacgaaccc
     2221 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2281 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2341 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     2401 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2461 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2521 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2581 cagtggaacg aaaactcacg ttaagggatt ttggtcatgg ctagtgcttg gattctcacc
     2641 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     2701 cattactgga tctatcaaca ggagtccaag ccaattcgta aacttggtct gacagttacc
     2761 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2821 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2881 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2941 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3001 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcagcgttg
     3061 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3121 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3181 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3241 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3301 ctggtgagta ttcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3361 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3421 ttggaaagcg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3481 cgatgtaacc cactcgcgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3541 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3601 aatgttgaat actcatactc ttcctttttc agtattattg aagcatttat cagggttatt
     3661 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3721 gcacatttcc ccgaaaagtg ccacct
//
