LOCUS       40924_20771        3585 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFYS018, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Scott FY, Ruder WC.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2016) Biological Systems Engineering, Virginia 
            Polytechnic Institute and State University, 1230 Washington Street 
            SW, Blacksburg, VA 24061, USA
REFERENCE   2  (bases 1 to 3585)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3585)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (21-SEP-2016) Biological Systems Engineering, Virginia Polytechnic 
            Institute and State University, 1230 Washington Street SW, 
            Blacksburg, VA 24061, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        151..435
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     regulatory      442..452
                     /regulatory_class="ribosome_binding_site"
     CDS             900..1445
                     /label=SNAP-tag(R)
                     /note="human O6-alkylguanine-DNA-alkyltransferase variant
                     that forms covalent bonds with benzylguanine derivatives"
     terminator      1545..1631
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(1795..2383)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(2471..2565)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2598..3455)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3456..3560)
                     /label=AmpR promoter
ORIGIN
        1 gacgtctgtg caagtactac tgttctgcag tcacttgagt tcgataccca gctgggtgga
       61 gtgcaccaag gagcatgcga aggaaacgtt tcgcagaagc ttccgcaagg taccactttg
      121 ccgcggagta tttgtacatt tgaaggatcc aagaaaccaa tagtccatat tgcatcagac
      181 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
      241 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaagcgcgt aacaaaagtg
      301 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
      361 ccatagcatt tttatccata agattagcgg atcatacctg acgcttttta tcgcaactct
      421 ctactgtttc tccatccatg gaggaaggag gagtcgacat gaaagctact aaactggtac
      481 tgggcgcggt aatcctgggt tctactctgc tggcaggttg ctccagcaac gctaaaatcg
      541 atcaggaatt caacccgtat gttggctttg aaatgggtta cgactggtta ggtcgtatgc
      601 cgtacaaagg cagcgttgaa aacggtgcat acaaagctca gggcgttcaa ctgaccgcta
      661 aactgggtta cccaatcact gacgacctgg acatctacac tcgtctgggt ggcatggtat
      721 ggcgtgcaga cactaaatcc aacgtttatg gtaaaaacca cgacaccggc gtttctccgg
      781 tcttcgctgg cggtgttgag tacgcgatca ctcctgaaat cgctacccgt ctggaatacc
      841 agtggaccaa caacatcggt gacgcacaca ccatcggcac tcgtccggac aacgagctca
      901 tggacaaaga ttgcgaaatg aaacgtacca ccctggatag cccgctgggc aaactggaac
      961 tgagcggctg cgaacagggc ctgcatgaaa ttaaactgct gggtaaaggc accagcgcgg
     1021 ccgatgcggt tgaagttccg gccccggccg ccgtgctggg tggtccggaa ccgctgatgc
     1081 aggcgaccgc gtggctgaac gcgtattttc atcagccgga agcgattgaa gaatttccgg
     1141 ttccggcgct gcatcatccg gtgtttcagc aggagagctt tacccgtcag gtgctgtgga
     1201 aactgctgaa agtggttaaa tttggcgaag tgattagcta tcagcagctg gcggccctgg
     1261 cgggtaatcc ggcggccacc gccgccgtta aaaccgcgct gagcggtaac ccggtgccga
     1321 ttctgattcc gtgccatcgt gtggttagct ctagcggtgc ggttggcggt tatgaaggtg
     1381 gtctggcggt gaaagagtgg ctgctggccc atgaaggtca tcgtctgggt aaaccgggtc
     1441 tgggataagc ccgggactac acaattgtcc cccggcgcca gggttgatat ctatcgccct
     1501 agggaccgtc tcgagagaat caatattaat ccaacgcgtg gcatcaaata aaacgaaagg
     1561 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     1621 gtaggacaaa tccgccgccc tagacttagg cgttcggctg cggcgagcgg tatcagctca
     1681 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1741 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1801 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1861 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1921 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1981 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2041 gggctgtctg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2101 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2161 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2221 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2281 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2341 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2401 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatggc
     2461 tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     2521 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc caattcgtaa
     2581 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     2641 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     2701 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     2761 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     2821 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     2881 aatagtttgc gcagcgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     2941 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3001 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3061 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3121 gtaagatgct tttctgtgac tggtgagtat tcaaccaagt cattctgaga atagtgtatg
     3181 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3241 actttaaaag tgctcatcat tggaaagcgt tcttcggggc gaaaactctc aaggatctta
     3301 ccgctgttga gatccagttc gatgtaaccc actcgcgcac ccaactgatc ttcagcatct
     3361 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     3421 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca gtattattga
     3481 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     3541 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacct
//
