LOCUS       V015196                 3813 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015196
VERSION     V015196
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..242
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     LTR             255..523
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             578..895
                     /label="hIg-kappa-CL"
                     /note="Human immunoglobulin kappa light chain constant
                     region"
     polyA_signal    complement(1173..1567)
                     /label="beta-globin poly(A)"
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(1579..1998)
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     enhancer        complement(2578..2881)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(2961..3549)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     polyA_signal    3657..3705
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    3716..3807
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
       61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
      121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
      301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
      361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
      421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
      481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
      541 ctacctgaga tcaccggtca ccatggaaat caaacgtacg gtggctgcac catctgtctt
      601 catcttcccg ccatctgatg agcagttgaa atctggaact gcctctgttg tgtgcctgct
      661 gaataacttc tatcccagag aggccaaagt acagtggaag gtggataacg ccctccaatc
      721 gggtaactcc caggagagtg tcacagagca ggacagcaag gacagcacct acagcctcag
      781 cagcaccctg acgctgagca aagcagacta cgagaaacac aaagtctacg cctgcgaagt
      841 cacccatcag ggcctgagct cgcccgtcac aaagagcttc aacaggggag agtgttagag
      901 ggagctagct cgacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
      961 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtgaaatttg
     1021 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     1081 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     1141 aaacctctac aaatgtggta tggaattaat tctaaaatac agcatagcaa aactttaacc
     1201 tccaaatcaa gcctctactt gaatcctttt ctgagggatg aataaggcat aggcatcagg
     1261 ggctgttgcc aatgtgcatt agctgtttgc agcctcacct tctttcatgg agtttaagat
     1321 atagtgtatt ttcccaaggt ttgaactagc tcttcatttc tttatgtttt aaatgcactg
     1381 acctcccaca ttcccttttt agtaaaatat tcagaaataa tttaaataca tcattgcaat
     1441 gaaaataaat gttttttatt aggcagaatc cagatgctca aggcccttca taatatcccc
     1501 cagtttagta gttggactta gggaacaaag gaacctttaa tagaaattgg acagcaagaa
     1561 agcgagcttc tagctttagt tcctggtgta cttgaggggg atgagttcct caatggtggt
     1621 tttgaccagc ttgccattca tctcaatgag cacaaagcag tcaggagcat agtcagagat
     1681 gagctctctg cacatgccac aggggctgac caccctgatg gatctgtcca cctcatcaga
     1741 gtaggggtgc ctgacagcca caatggtgtc aaagtccttc tgcccgttgc tcacagcaga
     1801 cccaatggca atggcttcag cacagacagt gaccctgcca atgtaggcct caatgtggac
     1861 agcagagatg atctccccag tcttggtcct gatggccgcc ccgacatggt gcttgttgtc
     1921 ctcatagagc atggtgatct tctcagtggc gacctccacc agctccagat cctgctgaga
     1981 gatgttgaag gtcttcatga tggctcctcc tgtcaggaga ggaaagagaa gaaggttagt
     2041 acaattgcta tagtgagttg tattatacta tgcttatgat taattgtcaa actagggctg
     2101 cagggttcat agtgccactt ttcctgcact gccccatctc ctgcccaccc tttcccaggc
     2161 atagacagtc agtgacttac caaactcaca ggagggagaa ggcagaagct tgagacagac
     2221 ccgcgggacc gccgaactgc gaggggacgt ggctagggcg gcttctttta tggtgcgccg
     2281 gccctcggag gcagggcgct cggggaggcc tagcggccaa tctgcggtgg caggaggcgg
     2341 ggccgaaggc cgtgcctgac caatccggag cacataggag tctcagcccc ccgccccaaa
     2401 gcaaggggaa gtcacgcgcc tgtagcgcca gcgtgttgtg aaatgggggc ttgggggggt
     2461 tggggccctg actagtcaaa acaaactccc attgacgtca atggggtgga gacttggaaa
     2521 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcatcat
     2581 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
     2641 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
     2701 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     2761 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
     2821 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     2881 gcctgcaggt taattaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2941 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3001 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3061 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3121 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3181 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3241 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3301 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3361 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3421 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3481 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3541 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3601 tcacgttaag ggattttggt catggctagt taattaacat ttaaatcagc ggccgcaata
     3661 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg taactaacat
     3721 acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct gtccccagtg
     3781 caagtgcagg tgccagaaca tttctctatc gaa
//
