LOCUS       40924_20741        4202 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pFUSE-rFc2-adapt-scFv, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Moutel S, El Marjou A, Vielemeyer O, Nizak C, Benaroch P, Dubel S, 
            Perez F.
  TITLE     A multi-Fc-species system for recombinant antibody production
  JOURNAL   BMC Biotechnol. 9, 14 (2009)
  PUBMED    19245715
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Perez F, Moutel S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2009) CNRS UMR144, Institut Curie / CNRS, 26, rue 
            d'Ulm, Pa, Paris 75005, France
REFERENCE   3  (bases 1 to 4202)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4202)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol. 9, 14 (2009)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-FEB-2009) CNRS UMR144, Institut Curie / CNRS, 26, rue d'Ulm, Pa,
            Paris 75005, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..246
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             259..527
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     577..636
                     /label=IL-2 signal sequence
                     /note="human interleukin-2 signal sequence"
     misc_feature    644..649
                     /note="NcoI restriction site: upstream insertion site for
                     scFv"
     misc_feature    661..668
                     /note="NotI restriction site; downstream insertion site for
                     scFv"
     misc_feature    676..1347
                     /label=Fc domain derived from rabbit immunoglobulin IgG2
                     /note="Fc domain derived from rabbit immunoglobulin IgG2"
     polyA_signal    complement(1367..1488)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(1592..1986)
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(2003..2374)
                     /label=BleoR
                     /note="antibiotic-binding protein"
     enhancer        complement(2967..3270)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3350..3938)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4050..4098
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4109..4200
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
       61 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
      121 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
      181 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
      241 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
      301 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
      361 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
      421 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
      481 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
      541 gcgcctacct gagatcaccg gcgaaggagg gccaccatgt acaggatgca actcctgtct
      601 tgcattgcac taagtcttgc acttgtcacg aattcgatat cggccatggt ttggtacctt
      661 gcggccgcta gatctagcaa gcccacgtgc ccaccccctg aactcctggg gggaccgtct
      721 gtcttcatct tccccccaaa acccaaggac accctcatga tctcacgcac ccccgaggtc
      781 acatgcgtgg tggtggacgt gagccaggat gaccccgagg tgcagttcac atggtacata
      841 aacaacgagc aggtgcgcac cgcccggccg ccgctacggg agcagcagtt caacagcacg
      901 atccgcgtgg tcagcaccct ccccatcgcg caccaggact ggctgagggg caaggagttc
      961 aagtgcaaag tccacaacaa ggcactcccg gcccccatcg agaaaaccat ctccaaagcc
     1021 agagggcagc ccctggagcc gaaggtctac acaatgggcc ctccccggga ggagctgagc
     1081 agcaggtcgg tcagcctgac ctgcatgatc aacggcttct acccttccga catctcggtg
     1141 gagtgggaga agaacgggaa ggcagaggac aactacaaga ccacgccggc cgtgctggac
     1201 agcgacggct cctacttcct ctacagcaag ctctcagtgc ccacgagtga gtggcagcgg
     1261 ggcgacgtct tcacctgctc cgtgatgcac gaggccttgc acaaccacta cacgcagaag
     1321 tccatctccc gctctccggg taaatgagct agctggccag acatgataag atacattgat
     1381 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     1441 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     1501 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     1561 aacctctaca aatgtggtat ggaattaatt ctaaaataca gcatagcaaa actttaacct
     1621 ccaaatcaag cctctacttg aatccttttc tgagggatga ataaggcata ggcatcaggg
     1681 gctgttgcca atgtgcatta gctgtttgca gcctcacctt ctttcatgga gtttaagata
     1741 tagtgtattt tcccaaggtt tgaactagct cttcatttct ttatgtttta aatgcactga
     1801 cctcccacat tcccttttta gtaaaatatt cagaaataat ttaaatacat cattgcaatg
     1861 aaaataaatg ttttttatta ggcagaatcc agatgctcaa ggcccttcat aatatccccc
     1921 agtttagtag ttggacttag ggaacaaagg aacctttaat agaaattgga cagcaagaaa
     1981 gcgagcttct agcttatcct cagtcctgct cctctgccac aaagtgcacg cagttgccgg
     2041 ccgggtcgcg cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg
     2101 gcccggaggc gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt
     2161 ccaggccgcg cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg
     2221 cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt
     2281 cccgggagaa cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg
     2341 tgagcaccgg aacggcactg gtcaacttgg ccatgatggc tcctcctgtc aggagaggaa
     2401 agagaagaag gttagtacaa ttgctatagt gagttgtatt atactatgca gatatactat
     2461 gccaatgatt aattgtcaaa ctagggctgc agggttcata gtgccacttt tcctgcactg
     2521 ccccatctcc tgcccaccct ttcccaggca tagacagtca gtgacttacc aaactcacag
     2581 gagggagaag gcagaagctt gagacagacc cgcgggaccg ccgaactgcg aggggacgtg
     2641 gctagggcgg cttcttttat ggtgcgccgg ccctcggagg cagggcgctc ggggaggcct
     2701 agcggccaat ctgcggtggc aggaggcggg gccgaaggcc gtgcctgacc aatccggagc
     2761 acataggagt ctcagccccc cgccccaaag caaggggaag tcacgcgcct gtagcgccag
     2821 cgtgttgtga aatgggggct tgggggggtt ggggccctga ctagtcaaaa caaactccca
     2881 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
     2941 ttgatgtact gccaaaaccg catcatcatg gtaatagcga tgactaatac gtagatgtac
     3001 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
     3061 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
     3121 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
     3181 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
     3241 ggcgggccat ttaccgtaag ttatgtaacg cctgcaggtt aattaagaac atgtgagcaa
     3301 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3361 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3421 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3481 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3541 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3601 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3661 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3721 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3781 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3841 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3901 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3961 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atggctagtt
     4021 aattaacatt taaatcagcg gccggccgca ataaaatatc tttattttca ttacatctgt
     4081 gtgttggttt tttgtgtgaa tcgtaactaa catacgctct ccatcaaaac aaaacgaaac
     4141 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
     4201 at
//
