LOCUS       62056_11030        4187 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..242
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             255..523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     573..632
                     /label=IL-2 signal sequence
                     /note="human interleukin-2 signal sequence"
     polyA_signal    complement(1352..1473)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(1577..1971)
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(1988..2359)
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        complement(2952..3255)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3335..3923)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4031..4079
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4090..4181
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
       61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
      121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
      301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
      361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
      421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
      481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
      541 ctacctgaga tcaccggcga aggagggcca ccatgtacag gatgcaactc ctgtcttgca
      601 ttgcactaag tcttgcactt gtcacgaatt cgatatcggc catggttaga tctggttgta
      661 agccttgcat atgtacagtc ccagaagtat catctgtctt catcttcccc ccaaagccca
      721 aggatgtgct caccattact ctgactccta aggtcacgtg tgttgtggta gacatcagca
      781 aggatgatcc cgaggtccag ttcagctggt ttgtagatga tgtggaggtg cacacagctc
      841 agacgcaacc ccgggaggag cagttcaaca gcactttccg ctcagtcagt gaacttccca
      901 tcatgcacca ggactggctc aatggcaagg agttcaaatg cagggtcaac agtgcagctt
      961 tccctgcccc catcgagaaa accatctcca aaaccaaagg cagaccgaag gctccacagg
     1021 tgtacaccat tccacctccc aaggagcaga tggccaagga taaagtcagt ctgacctgca
     1081 tgataacaga cttcttccct gaagacatta ctgtggagtg gcagtggaat gggcagccag
     1141 cggagaacta caagaacact cagcccatca tggacacaga tggctcttac ttcgtctaca
     1201 gcaagctcaa tgtgcagaag agcaactggg aggcaggaaa tactttcacc tgctctgtgt
     1261 tacatgaggg cctgcacaac caccatactg agaagagcct ctcccactct cctggtaaat
     1321 gatcccagtg tcgctagctg gccagacatg ataagataca ttgatgagtt tggacaaacc
     1381 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     1441 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     1501 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     1561 ggtatggaat taattctaaa atacagcata gcaaaacttt aacctccaaa tcaagcctct
     1621 acttgaatcc ttttctgagg gatgaataag gcataggcat caggggctgt tgccaatgtg
     1681 cattagctgt ttgcagcctc accttctttc atggagttta agatatagtg tattttccca
     1741 aggtttgaac tagctcttca tttctttatg ttttaaatgc actgacctcc cacattccct
     1801 ttttagtaaa atattcagaa ataatttaaa tacatcattg caatgaaaat aaatgttttt
     1861 tattaggcag aatccagatg ctcaaggccc ttcataatat cccccagttt agtagttgga
     1921 cttagggaac aaaggaacct ttaatagaaa ttggacagca agaaagcgag cttctagctt
     1981 atcctcagtc ctgctcctct gccacaaagt gcacgcagtt gccggccggg tcgcgcaggg
     2041 cgaactcccg cccccacggc tgctcgccga tctcggtcat ggccggcccg gaggcgtccc
     2101 ggaagttcgt ggacacgacc tccgaccact cggcgtacag ctcgtccagg ccgcgcaccc
     2161 acacccaggc cagggtgttg tccggcacca cctggtcctg gaccgcgctg atgaacaggg
     2221 tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac gaagtcccgg gagaacccga
     2281 gccggtcggt ccagaactcg accgctccgg cgacgtcgcg cgcggtgagc accggaacgg
     2341 cactggtcaa cttggccatg atggctcctc ctgtcaggag aggaaagaga agaaggttag
     2401 tacaattgct atagtgagtt gtattatact atgcagatat actatgccaa tgattaattg
     2461 tcaaactagg gctgcagggt tcatagtgcc acttttcctg cactgcccca tctcctgccc
     2521 accctttccc aggcatagac agtcagtgac ttaccaaact cacaggaggg agaaggcaga
     2581 agcttgagac agacccgcgg gaccgccgaa ctgcgagggg acgtggctag ggcggcttct
     2641 tttatggtgc gccggccctc ggaggcaggg cgctcgggga ggcctagcgg ccaatctgcg
     2701 gtggcaggag gcggggccga aggccgtgcc tgaccaatcc ggagcacata ggagtctcag
     2761 ccccccgccc caaagcaagg ggaagtcacg cgcctgtagc gccagcgtgt tgtgaaatgg
     2821 gggcttgggg gggttggggc cctgactagt caaaacaaac tcccattgac gtcaatgggg
     2881 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
     2941 aaccgcatca tcatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
     3001 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
     3061 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
     3121 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
     3181 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
     3241 gtaagttatg taacgcctgc aggttaatta agaacatgtg agcaaaaggc cagcaaaagg
     3301 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3361 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3421 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3481 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3541 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3601 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3661 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3721 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3781 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3841 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3901 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3961 agtggaacga aaactcacgt taagggattt tggtcatggc tagttaatta acatttaaat
     4021 cagcggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
     4081 atcgtaacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata
     4141 ggctgtcccc agtgcaagtg caggtgccag aacatttctc tatcgaa
//
