LOCUS       62056_11025        4184 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..242
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             255..523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     573..632
                     /label=IL-2 signal sequence
                     /note="human interleukin-2 signal sequence"
     polyA_signal    complement(1349..1470)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(1574..1968)
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(1985..2356)
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        complement(2949..3252)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3332..3920)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4028..4076
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4087..4178
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
       61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
      121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
      301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
      361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
      421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
      481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
      541 ctacctgaga tcaccggcga aggagggcca ccatgtacag gatgcaactc ctgtcttgca
      601 ttgcactaag tcttgcactt gtcacgaatt cgatatcggc catggttaga tctcccccat
      661 gcccatcatg cccagcacct gagttcctgg ggggaccatc agtcttcctg ttccccccaa
      721 aacccaagga cactctcatg atctcccgga cccctgaggt cacgtgcgtg gtggtggacg
      781 tgagccagga agaccccgag gtccagttca actggtacgt ggatggcgtg gaggtgcata
      841 atgccaagac aaagccgcgg gaggagcagt tcaacagcac gtaccgtgtg gtcagcgtcc
      901 tcaccgtcct gcaccaggac tggctgaacg gcaaggagta caagtgcaag gtctccaaca
      961 aaggcctccc gtcctccatc gagaaaacca tctccaaagc caaagggcag ccccgagagc
     1021 cacaggtgta caccctgccc ccatcccagg aggagatgac caagaaccag gtcagcctga
     1081 cctgcctggt caaaggcttc taccccagcg acatcgccgt ggagtgggag agcaatgggc
     1141 agccggagaa caactacaag accacgcctc ccgtgctgga ctccgacggc tccttcttcc
     1201 tctacagcag gctaaccgtg gacaagagca ggtggcagga ggggaatgtc ttctcatgct
     1261 ccgtgatgca tgaggctctg cacaaccact acacacagag agcctctccc tgtctccggg
     1321 taaatgagtg ctagctggcc agacatgata agatacattg atgagtttgg acaaaccaca
     1381 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     1441 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     1501 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     1561 atggaattaa ttctaaaata cagcatagca aaactttaac ctccaaatca agcctctact
     1621 tgaatccttt tctgagggat gaataaggca taggcatcag gggctgttgc caatgtgcat
     1681 tagctgtttg cagcctcacc ttctttcatg gagtttaaga tatagtgtat tttcccaagg
     1741 tttgaactag ctcttcattt ctttatgttt taaatgcact gacctcccac attccctttt
     1801 tagtaaaata ttcagaaata atttaaatac atcattgcaa tgaaaataaa tgttttttat
     1861 taggcagaat ccagatgctc aaggcccttc ataatatccc ccagtttagt agttggactt
     1921 agggaacaaa ggaaccttta atagaaattg gacagcaaga aagcgagctt ctagcttatc
     1981 ctcagtcctg ctcctctgcc acaaagtgca cgcagttgcc ggccgggtcg cgcagggcga
     2041 actcccgccc ccacggctgc tcgccgatct cggtcatggc cggcccggag gcgtcccgga
     2101 agttcgtgga cacgacctcc gaccactcgg cgtacagctc gtccaggccg cgcacccaca
     2161 cccaggccag ggtgttgtcc ggcaccacct ggtcctggac cgcgctgatg aacagggtca
     2221 cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag aacccgagcc
     2281 ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc ggaacggcac
     2341 tggtcaactt ggccatgatg gctcctcctg tcaggagagg aaagagaaga aggttagtac
     2401 aattgctata gtgagttgta ttatactatg cagatatact atgccaatga ttaattgtca
     2461 aactagggct gcagggttca tagtgccact tttcctgcac tgccccatct cctgcccacc
     2521 ctttcccagg catagacagt cagtgactta ccaaactcac aggagggaga aggcagaagc
     2581 ttgagacaga cccgcgggac cgccgaactg cgaggggacg tggctagggc ggcttctttt
     2641 atggtgcgcc ggccctcgga ggcagggcgc tcggggaggc ctagcggcca atctgcggtg
     2701 gcaggaggcg gggccgaagg ccgtgcctga ccaatccgga gcacatagga gtctcagccc
     2761 cccgccccaa agcaagggga agtcacgcgc ctgtagcgcc agcgtgttgt gaaatggggg
     2821 cttggggggg ttggggccct gactagtcaa aacaaactcc cattgacgtc aatggggtgg
     2881 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     2941 cgcatcatca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     3001 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     3061 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     3121 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     3181 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     3241 agttatgtaa cgcctgcagg ttaattaaga acatgtgagc aaaaggccag caaaaggcca
     3301 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3361 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3421 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3481 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3541 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3601 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3661 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3721 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     3781 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3841 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3901 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3961 ggaacgaaaa ctcacgttaa gggattttgg tcatggctag ttaattaaca tttaaatcag
     4021 cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
     4081 gtaactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc
     4141 tgtccccagt gcaagtgcag gtgccagaac atttctctat cgaa
//
