LOCUS       62056_11015        4587 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..242
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             255..523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     573..632
                     /label=IL-2 signal sequence
                     /note="human interleukin-2 signal sequence"
     CDS             735..1724
                     /codon_start=1
                     /label=hlgG1-CH
                     /note="Human IgG1 heavy chain constant region"
                     /translation="ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGA
                     LTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD
                     KTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVD
                     GVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKA
                     KGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVL
                     DSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK"
     polyA_signal    complement(1752..1873)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(1977..2371)
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(2388..2759)
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        complement(3352..3655)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3735..4323)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4431..4479
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4490..4581
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
       61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
      121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
      301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
      361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
      421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
      481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
      541 ctacctgaga tcaccggcga aggagggcca ccatgtacag gatgcaactc ctgtcttgca
      601 ttgcactaag tcttgcactt gtcacgaatt cgatatcggc catggttaga tctatggact
      661 ggacctggag ggtgttctgc ctgctggccg tggcccccgg cgcccacagc gaagagctac
      721 agagtgagct cttcgccagc accaagggtc caagtgtatt cccactcgct ccaagctcta
      781 aatccacgag cggtggtacg gcagccctcg gatgcctcgt caaagattat tttccagaac
      841 ctgttacagt atcttggaat agcggcgcgt tgaccagtgg cgtccataca tttcccgcgg
      901 tattgcaatc atctggcctc tatagtcttt catcagtggt caccgtcccg agttctagcc
      961 tgggtacaca gacttacatt tgcaatgtaa accataagcc aagcaatact aaagttgaca
     1021 agaaggtcga gcccaaaagc tgtgacaaga ctcatacctg cccgccctgc ccagcccccg
     1081 aacttcttgg tggaccgtcc gtcttcctct ttcctcctaa gccgaaggac acgctgatga
     1141 tctcaaggac tcccgaagtt acatgtgtgg tcgtagatgt ctcacacgag gaccccgagg
     1201 ttaaattcaa ttggtacgtc gacggagtag aagttcataa cgccaaaact aagccgaggg
     1261 aggagcaata caattcaacg tatcgggtcg taagcgttct taccgtcctc catcaggatt
     1321 ggttgaacgg taaagaatac aagtgcaaag ttagcaataa agccctcccc gccccgatag
     1381 aaaagactat aagtaaggcg aaaggacaac caagagaacc ccaagtttac actttgcccc
     1441 cctcacggga tgaacttaca aaaaatcaag tctcccttac atgcctggtg aagggtttct
     1501 acccatcaga tatcgcagtg gaatgggaat ctaacggaca accagaaaat aattacaaga
     1561 cgacgccgcc agtcctcgac tctgatgggt cctttttttt gtactcaaaa cttactgtgg
     1621 ataaaagtag gtggcaacaa ggcaatgtgt tctcctgctc agtgatgcat gaggcccttc
     1681 acaatcatta tacccagaaa tcattgagcc tgagccccgg caaatgatga gtgctagctg
     1741 gccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     1801 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     1861 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     1921 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggaat taattctaaa
     1981 atacagcata gcaaaacttt aacctccaaa tcaagcctct acttgaatcc ttttctgagg
     2041 gatgaataag gcataggcat caggggctgt tgccaatgtg cattagctgt ttgcagcctc
     2101 accttctttc atggagttta agatatagtg tattttccca aggtttgaac tagctcttca
     2161 tttctttatg ttttaaatgc actgacctcc cacattccct ttttagtaaa atattcagaa
     2221 ataatttaaa tacatcattg caatgaaaat aaatgttttt tattaggcag aatccagatg
     2281 ctcaaggccc ttcataatat cccccagttt agtagttgga cttagggaac aaaggaacct
     2341 ttaatagaaa ttggacagca agaaagcgag cttctagctt atcctcagtc ctgctcctct
     2401 gccacaaagt gcacgcagtt gccggccggg tcgcgcaggg cgaactcccg cccccacggc
     2461 tgctcgccga tctcggtcat ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc
     2521 tccgaccact cggcgtacag ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg
     2581 tccggcacca cctggtcctg gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca
     2641 ccggcgaagt cgtcctccac gaagtcccgg gagaacccga gccggtcggt ccagaactcg
     2701 accgctccgg cgacgtcgcg cgcggtgagc accggaacgg cactggtcaa cttggccatg
     2761 atggctcctc ctgtcaggag aggaaagaga agaaggttag tacaattgct atagtgagtt
     2821 gtattatact atgcagatat actatgccaa tgattaattg tcaaactagg gctgcagggt
     2881 tcatagtgcc acttttcctg cactgcccca tctcctgccc accctttccc aggcatagac
     2941 agtcagtgac ttaccaaact cacaggaggg agaaggcaga agcttgagac agacccgcgg
     3001 gaccgccgaa ctgcgagggg acgtggctag ggcggcttct tttatggtgc gccggccctc
     3061 ggaggcaggg cgctcgggga ggcctagcgg ccaatctgcg gtggcaggag gcggggccga
     3121 aggccgtgcc tgaccaatcc ggagcacata ggagtctcag ccccccgccc caaagcaagg
     3181 ggaagtcacg cgcctgtagc gccagcgtgt tgtgaaatgg gggcttgggg gggttggggc
     3241 cctgactagt caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
     3301 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca tcatggtaat
     3361 agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg
     3421 ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat
     3481 atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg
     3541 tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg
     3601 gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcctgc
     3661 aggttaatta agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3721 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3781 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3841 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3901 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3961 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4021 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4081 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4141 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4201 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4261 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4321 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4381 taagggattt tggtcatggc tagttaatta acatttaaat cagcggccgc aataaaatat
     4441 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgtaacta acatacgctc
     4501 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
     4561 caggtgccag aacatttctc tatcgaa
//
