LOCUS       40924_20726        4210 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pFUSE-hFc2-adapt-scFv, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Moutel S, El Marjou A, Vielemeyer O, Nizak C, Benaroch P, Dubel S, 
            Perez F.
  TITLE     A multi-Fc-species system for recombinant antibody production
  JOURNAL   BMC Biotechnol. 9, 14 (2009)
  PUBMED    19245715
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Perez F, Moutel S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2009) CNRS UMR144, Institut Curie / CNRS, 26, rue 
            d'Ulm, Pa, Paris 75005, France
REFERENCE   3  (bases 1 to 4210)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4210)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol. 9, 14 (2009)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-FEB-2009) CNRS UMR144, Institut Curie / CNRS, 26, rue d'Ulm, Pa,
            Paris 75005, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..246
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             259..527
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     577..636
                     /label=IL-2 signal sequence
                     /note="human interleukin-2 signal sequence"
     misc_feature    644..649
                     /note="NcoI restriction site; upstream insertion site for
                     scFv"
     misc_feature    661..668
                     /note="NotI restriction site; downstream insertion site for
                     scFv"
     misc_feature    676..1347
                     /note="Fc domain derived from Homo sapiens immunoglobulin
                     IgG2"
     polyA_signal    complement(1375..1496)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(1600..1994)
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(2011..2382)
                     /label=BleoR
                     /note="antibiotic-binding protein"
     enhancer        complement(2975..3278)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3358..3946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4058..4106
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4117..4208
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
       61 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
      121 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
      181 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
      241 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
      301 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
      361 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
      421 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
      481 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
      541 gcgcctacct gagatcaccg gcgaaggagg gccaccatgt acaggatgca actcctgtct
      601 tgcattgcac taagtcttgc acttgtcacg aattcgatat cggccatggt ttggtacctt
      661 gcggccgcta gatctgtgga gtgcccacct tgcccagcac cacctgtggc aggaccttca
      721 gtcttcctct tccccccaaa acccaaggac accctgatga tctccagaac ccctgaggtc
      781 acgtgcgtgg tggtggacgt gagccacgaa gaccccgagg tccagttcaa ctggtacgtg
      841 gacggcatgg aggtgcataa tgccaagaca aagccacggg aggagcagtt caacagcacg
      901 ttccgtgtgg tcagcgtcct caccgtcgtg caccaggact ggctgaacgg caaggagtac
      961 aagtgcaagg tctccaacaa aggcctccca gcccccatcg agaaaaccat ctccaaaacc
     1021 aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga ggagatgacc
     1081 aagaaccagg tcagcctgac ctgcctggtc aaaggcttct accccagcga catcgccgtg
     1141 gagtgggaga gcaatgggca gccggagaac aactacaaga ccacacctcc catgctggac
     1201 tccgacggct ccttcttcct ctacagcaag ctcaccgtgg acaagagcag gtggcagcag
     1261 gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacacagaag
     1321 agcctctccc tgtctccggg taaatgagtg ccacggctag ctggccagac atgataagat
     1381 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     1441 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     1501 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     1561 gcaagtaaaa cctctacaaa tgtggtatgg aattaattct aaaatacagc atagcaaaac
     1621 tttaacctcc aaatcaagcc tctacttgaa tccttttctg agggatgaat aaggcatagg
     1681 catcaggggc tgttgccaat gtgcattagc tgtttgcagc ctcaccttct ttcatggagt
     1741 ttaagatata gtgtattttc ccaaggtttg aactagctct tcatttcttt atgttttaaa
     1801 tgcactgacc tcccacattc cctttttagt aaaatattca gaaataattt aaatacatca
     1861 ttgcaatgaa aataaatgtt ttttattagg cagaatccag atgctcaagg cccttcataa
     1921 tatcccccag tttagtagtt ggacttaggg aacaaaggaa cctttaatag aaattggaca
     1981 gcaagaaagc gagcttctag cttatcctca gtcctgctcc tctgccacaa agtgcacgca
     2041 gttgccggcc gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc cgatctcggt
     2101 catggccggc ccggaggcgt cccggaagtt cgtggacacg acctccgacc actcggcgta
     2161 cagctcgtcc aggccgcgca cccacaccca ggccagggtg ttgtccggca ccacctggtc
     2221 ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc acaccggcga agtcgtcctc
     2281 cacgaagtcc cgggagaacc cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc
     2341 gcgcgcggtg agcaccggaa cggcactggt caacttggcc atgatggctc ctcctgtcag
     2401 gagaggaaag agaagaaggt tagtacaatt gctatagtga gttgtattat actatgcaga
     2461 tatactatgc caatgattaa ttgtcaaact agggctgcag ggttcatagt gccacttttc
     2521 ctgcactgcc ccatctcctg cccacccttt cccaggcata gacagtcagt gacttaccaa
     2581 actcacagga gggagaaggc agaagcttga gacagacccg cgggaccgcc gaactgcgag
     2641 gggacgtggc tagggcggct tcttttatgg tgcgccggcc ctcggaggca gggcgctcgg
     2701 ggaggcctag cggccaatct gcggtggcag gaggcggggc cgaaggccgt gcctgaccaa
     2761 tccggagcac ataggagtct cagccccccg ccccaaagca aggggaagtc acgcgcctgt
     2821 agcgccagcg tgttgtgaaa tgggggcttg ggggggttgg ggccctgact agtcaaaaca
     2881 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc
     2941 cacgcccatt gatgtactgc caaaaccgca tcatcatggt aatagcgatg actaatacgt
     3001 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg
     3061 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta
     3121 ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta
     3181 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg
     3241 gtcagccagg cgggccattt accgtaagtt atgtaacgcc tgcaggttaa ttaagaacat
     3301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3361 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3781 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3841 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3961 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4021 ggctagttaa ttaacattta aatcagcggc cggccgcaat aaaatatctt tattttcatt
     4081 acatctgtgt gttggttttt tgtgtgaatc gtaactaaca tacgctctcc atcaaaacaa
     4141 aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac
     4201 atttctctat
//
