LOCUS       V006154                 8072 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006154
VERSION     V006154
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Poquet I, Ehrlich SD, Gruss A.
  TITLE     An export-specific reporter designed for gram-positive bacteria:
            application to Lactococcus lactis
  JOURNAL   J. Bacteriol. 180 (7), 1904-1912 (1998)
   PUBMED   9537391
REFERENCE   2  (bases 1 to 8072)
  AUTHORS   Poquet I, Gruss A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-1997) Laboratoire de Genetique Appliquee-URLGA,
            Institut National de la Recherche Agronomique, CRJ, Jouy en Josas
            78352, France
REFERENCE   3  (bases 1 to 8072)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8072)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "1998"; volume: "180"; issue: "7"; pages:
            "1904-1912"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-DEC-1997) Laboratoire de Genetique Appliquee-URLGA, Institut
            National de la Recherche Agronomique, CRJ, Jouy en Josas 78352,
            France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             539..1273
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     promoter        complement(4599..4617)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4624..4640)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(4781..5236)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5263..5367
                     /label="AmpR promoter"
     CDS             5368..6225
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6399..6987
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7275..7296
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7311..7341
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7349..7365
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7373..7389
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7410..7428
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     7458..7474
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      7473..7507
                     /label="trpA terminator"
                     /note="trpA terminator"
                     /regulatory_class="terminator"
     misc_feature    7512..7547
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     CDS             7561..8007
                     /label="MNase"
                     /note="micrococcal nuclease from Staphylococcus aureus"
ORIGIN
        1 ctagaatcga tacgattttg aagtggcaac agataaaaaa aagcagttta aaattgttgc
       61 tgaactttta aaacaagcaa atacaatcat tgtcgcaaca gatagcgaca gagaaggcga
      121 aaacattgcc tggtcgatca ttcataaagc aaatgccttt tctaaagata aaacgtataa
      181 aagactatgg atcaatagtt tagaaaaaga tgtgatccgt agcggttttc aaaatttgca
      241 accaggaatg aattactatc ccttttatca agaagcgcaa aagaaaaacg aaatgataca
      301 ccaatcagtg caaaaaaaga tataatggga gataagacgg ttcgtgttcg tgctgacttg
      361 caccatatca taaaaatcga aacagcaaag aatggcggaa acgtaaaaga agttatggaa
      421 ataagactta gaagcaaact taagagtgtg ttgatagtgc agtatcttaa aattttgtat
      481 aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gtgattacat
      541 gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat
      601 aataaaacaa ttgaatttaa aagaaaccga taccgtttac gaaattggaa caggtaaagg
      661 gcatttaacg acgaaactgg ctaaaataag taaacaggta acgtctattg aattagacag
      721 tcatctattc aacttatcgt cagaaaaatt aaaactgaat actcgtgtca ctttaattca
      781 ccaagatatt ctacagtttc aattccctaa caaacagagg tataaaattg ttgggagtat
      841 tccttaccat ttaagcacac aaattattaa aaaagtggtt tttgaaagcc atgcgtctga
      901 catctatctg attgttgaag aaggattcta caagcgtacc ttggatattc accgaacact
      961 agggttgctc ttgcacactc aagtctcgat tcagcaattg cttaagctgc cagcggaatg
     1021 ctttcatcct aaaccaaaag taaacagtgt cttaataaaa cttacccgcc ataccacaga
     1081 tgttccagat aaatattgga agctatatac gtactttgtt tcaaaatggg tcaatcgaga
     1141 atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa
     1201 caatttaagt accgttactt atgagcaagt attgtctatt tttaatagtt atctattatt
     1261 taacgggagg aaataattct atgagtcgct tttgtaaatt tggaaagtta cacgttacta
     1321 aagggaatgt agataaatta ttaggtatac tactgacagc ttccaaggag ctaaagaggt
     1381 ccctagcgct tagaatcgct ttaggaaaca cgatccagtc caataatcgt cgataaaaac
     1441 ttttgaaaaa ggttggtgaa attacctact tttggaataa tcacaaatca caagtgatta
     1501 atcacaaatc acaagtgatt aatcacttgt ttattaagat attaaaagct ataatttaaa
     1561 taaagcgtga attttattac acaaaaagag gggggagaaa cttggaacta gcatttagag
     1621 aaagcttaaa aaagatgaga ggtaccaaat caaaagaaaa attctcccaa gaattagaaa
     1681 tgagtagatc aaattattca cgaatagaat caggaaaatc agatccaacc ataaaaacac
     1741 tagaacaaat tgcaaagtta actaactcaa cgctagtagt ggatttaatc ccaaatgagc
     1801 caacagaacc agaaccagaa acagaatcag aacaagtaac attggattta gaaatggaag
     1861 aagaaaaaag caatgacttc gtgtgaataa tgcacgaaat cgttgcttat ttttttttaa
     1921 aagcggtata ctagatataa cgaaacaacg aactgaatag aaacgaaaaa agagccatga
     1981 cacatttata aaatgtttga cgacatttta taaatgcata gcccgataag attgccaaac
     2041 caacgcttat cagttagtca gatgaactct tccctcgtaa gaagttattt aattaacttt
     2101 gtttgaagac ggtatataac cgtactatca ttatataggg aaatcagaga gttttcaagt
     2161 atctaagcta ctgaatttaa gaattgttaa gcaatcaatc ggaaatcgtt tgattgcttt
     2221 ttttgtattc atttatagaa ggtggagttt gtatgaatca tgatgaatgt aaaacttata
     2281 taaaaaatag tttattggag ataagaaaat tagcaaatat ctatacacta gaaacgttta
     2341 agaaagagtt agaaaagaga aatatctact tagaaacaaa atcagataag tatttttctt
     2401 cggaggggga agattatata tataagttaa tagaaaataa caaaataatt tattcgatta
     2461 gtggaaaaaa attgacttat aaaggaaaaa aatctttttc aaaacatgca atattgaaac
     2521 agttgaatga aaaagcaaac caagttaatt aaacaaccta ttttatagga tttataggaa
     2581 aggagaacag ctgaatgaat atcccttttg ttgtagaaac tgtgcttcat gacggcttgt
     2641 taaagtacaa atttaaaaat agtaaaattc gctcaatcac taccaagcca ggtaaaagca
     2701 aaggggctat ttttgcgtat cgctcaaaat caagcatgat tggcggtcgt ggtgttgttc
     2761 tgacttccga ggaagcgatt caagaaaatc aagatacatt tacacattgg acacccaacg
     2821 tttatcgtta tggaacgtat gcagacgaaa accgttcata cacgaaagga cattctgaaa
     2881 acaatttaag acaaatcaat accttcttta ttgattttga tattcacacg gcaaaagaaa
     2941 ctatttcagc aagcgatatt ttaacaaccg ctattgattt aggttttatg cctactatga
     3001 ttatcaaatc tgataaaggt tatcaagcat attttgtttt agaaacgcca gtctatgtga
     3061 cttcaaaatc agaatttaaa tctgtcaaag cagccaaaat aatttcgcaa aatatccgag
     3121 aatattttgg aaagtctttg ccagttgatc taacgtgtaa tcattttggt attgctcgca
     3181 taccaagaac ggacaatgta gaattttttg atcctaatta ccgttattct ttcaaagaat
     3241 ggcaagattg gtctttcaaa caaacagata ataagggctt tactcgttca agtctaacgg
     3301 ttttaagcgg tacagaaggc aaaaaacaag tagatgaacc ctggtttaat ctcttattgc
     3361 acgaaacgaa attttcagga gaaaagggtt taatagggcg taataacgtc atgtttaccc
     3421 tctctttagc ctactttagt tcaggctatt caatcgaaac gtgcgaatat aatatgtttg
     3481 agtttaataa tcgattagat caacccttag aagaaaaaga agtaatcaaa attgttagaa
     3541 gtgcctattc agaaaactat caaggggcta atagggaata cattaccatt ctttgcaaag
     3601 cttgggtatc aagtgattta accagtaaag atttatttgt ccgtcaaggg tggtttaaat
     3661 tcaagaaaaa aagaagcgaa cgtcaacgtg ttcatttgtc agaatggaaa gaagatttaa
     3721 tggcttatat tagcgaaaaa agcgatgtat acaagcctta tttagtgacg accaaaaaag
     3781 agattagaga agtgctaggc attcctgaac ggacattaga taaattgctg aaggtactga
     3841 aggcgaatca ggaaattttc tttaagatta aaccaggaag aaatggtggc attcaacttg
     3901 ctagtgttaa atcattgttg ctatcgatca ttaaagtaaa aaaagaagaa aaagaaagct
     3961 atataaaggc gctgacaaat tcttttgact tagagcatac attcattcaa gagactttaa
     4021 acaagctagc agaacgccct aaaacggaca cacaactcga tttgtttagc tatgatacag
     4081 gctgaaaata aaacccgcac tatgccatta catttatatc tatgatacgt gtttgttttt
     4141 tctttgctgt ttagcgaatg attagcagaa atatacagag taagatttta attaattatt
     4201 agggggagaa ggagagagta gcccgaaaac ttttagttgg cttggactga acgaagtgag
     4261 ggaaaggcta ctaaaacgtc gaggggcagt gagagcgaag cgaacacttg attttttaat
     4321 tttctatctt ttataggtca ttagagtata cttatttgtc ctataaacta tttagcagca
     4381 taatagattt attgaatagg tcatttaagt tgagcatatt agaggaggaa aatcttggag
     4441 aaatatttga agaacccgat tacatggatt ggattagttc ttgtggttac gtggttttta
     4501 actaaaagta gtgaattttt gatttttggt gtgtgtgtct tgttgttagt atttgctagt
     4561 caaagtgatt aaatagaatt ggtggagctc caattcgccc tatagtgagt cgtattacaa
     4621 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     4681 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     4741 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aaattgtaag cgttaatatt
     4801 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     4861 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     4921 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     4981 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     5041 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     5101 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     5161 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     5221 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5281 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5341 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5401 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5461 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5521 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5581 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5641 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5701 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5761 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5821 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5881 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5941 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6001 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6061 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6121 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6181 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6241 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6301 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6361 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6421 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6481 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6541 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6601 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6661 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6721 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6781 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6841 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6901 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6961 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7021 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7081 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7141 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7201 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     7261 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     7321 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     7381 agctatgacc atgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg
     7441 ggtaccgggc cccccctcga ggtcgacggt atcgatagcc cgcctaatga gcgggctttt
     7501 ttttgatatc gaattacccg ggaattcaga tctttgatca aggatccaac agtatatagt
     7561 gcaacttcaa ctaaaaaatt acataaagaa cctgcgactt taattaaagc gattgatggt
     7621 gatacggtta aattaatgta caaaggtcaa ccaatgacat tcagactatt attggttgat
     7681 acacctgaaa caaagcatcc taaaaaaggt gtagagaaat atggtcctga agcaagtgca
     7741 tttacgaaaa aaatggtaga aaatgcaaag aaaattgaag tcgagtttga caaaggtcaa
     7801 agaactgata aatatggacg tggcttagcg tatatttatg ctgatggaaa aatggtaaac
     7861 gaagctttag ttcgtcaagg cttggctaaa gttgcttatg tttacaaacc taacaataca
     7921 catgaacaac atttaagaaa aagtgaagca caagcgaaaa aagagaaatt aaatatttgg
     7981 agcgaagaca acgctgattc aggtcaataa tgctcattgt aaaagtgtca ctgctgctag
     8041 tggcactttt ataattttta gatccactag tt
//
