LOCUS       40924_20706        6446 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter gene-fusion vector pFU99, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Uliczka F, Pisano F, Kochut A, Opitz W, Herbst K, Stolz T, Dersch P.
  TITLE     Monitoring of Gene Expression in Bacteria during Infections Using an
            Adaptable Set of Bioluminescent, Fluorescent and Colorigenic Fusion 
            Vectors
  JOURNAL   PLoS ONE 6 (6), E20425 (2011)
  PUBMED    21673990
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Uliczka F.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2011) Department of Molecular Infection Biology, 
            Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 
            Braunschweig, Niedersachsen 38124, Germany
REFERENCE   3  (bases 1 to 6446)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6446)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2011"; volume: "6"; issue: "6"; pages: "E20425"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-APR-2011) Department of Molecular Infection Biology, Helmholtz 
            Centre for Infection Research, Inhoffenstrasse 7, Braunschweig, 
            Niedersachsen 38124, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      36..42
                     /gene="lacZ"
                     /regulatory_class="ribosome_binding_site"
     CDS             44..3115
                     /label=lacZ
                     /note="beta-galactosidase"
     terminator      3138..3224
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(3762..4709)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(4757..4979)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(5471..5565)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(5589..6245)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(6246..6348)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacaggag gaattaaaaa tgaaagggaa
       61 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      121 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      181 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc
      241 accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt
      301 cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta
      361 tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct
      421 cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg
      481 cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag
      541 tcgtttgccg tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc
      601 ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa gatcaggata tgtggcggat
      661 gagcggcatt ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt
      721 ccatgttgcc actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca
      781 gatgtgcggc gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac
      841 gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta
      901 tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat
      961 cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc
     1021 agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa
     1081 cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca
     1141 ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa
     1201 cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta
     1261 cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg
     1321 tctgaccgat gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca
     1381 gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgaat caggccacgg
     1441 cgctaatcac gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca
     1501 gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg
     1561 cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc
     1621 gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag
     1681 tcttggcggt ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg
     1741 cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg
     1801 gtcggcttac ggcggtgatt ttggcgatac gccgaacgat cgccagttct gtatgaacgg
     1861 tctggtcttt gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac accagcagca
     1921 gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg
     1981 tcatagcgat aacgaactcc tgcactggat ggtggcgctg gatggtaagc cgctggcaag
     2041 cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac tgcctgaact
     2101 accgcagccg gagagcgccg ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc
     2161 gaccgcatgg tcagaagccg ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa
     2221 cctcagtgtg acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat
     2281 ggatttttgc atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct
     2341 ttcacagatg tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac
     2401 ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc
     2461 ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg
     2521 cacggcagat acacttgctg atgcggtgct gattacgacc gctcacgcgt ggcagcatca
     2581 ggggaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc
     2641 gattaccgtt gatgttgaag tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa
     2701 ctgccagctg gcgcaggtag cagagcgggt aaactggctc ggattagggc cgcaagaaaa
     2761 ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat
     2821 gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa
     2881 ttatggccca caccagtggc gcggcgactt ccagttcaac atcagccgct acagtcaaca
     2941 gcaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa
     3001 tatcgacggt ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtatcggc
     3061 ggaattacag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataagc
     3121 ggccgctcta gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     3181 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ccctagacct
     3241 agggtacggg ttttgctgcc cgcaaacggg ctgttctggt gttgctagtt tgttatcaga
     3301 atcgcagatc cggcttcagg tttgccggct gaaagcgcta tttcttccag aattgccatg
     3361 attttttccc cacgggaggc gtcactggct cccgtgttgt cggcagcttt gattcgataa
     3421 gcagcatcgc ctgtttcagg ctgtctatgt gtgactgttg agctgtaaca agttgtctca
     3481 ggtgttcaat ttcatgttct agttgctttg ttttactggt ttcacctgtt ctattaggtg
     3541 ttacatgctg ttcatctgtt acattgtcga tctgttcatg gtgaacagct ttaaatgcac
     3601 caaaaactcg taaaagctct gatgtatcta tcttttttac accgttttca tctgtgcata
     3661 tggacagttt tccctttgat atctaacggt gaacagttgt tctacttttg tttgttagtc
     3721 ttgatgcttc actgatagat acaagagcca taagaacctc agatccttcc gtatttagcc
     3781 agtatgttct ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga accattgaga
     3841 tcatgcttac tttgcatgtc actcaaaaat tttgcctcaa aactggtgag ctgaattttt
     3901 gcagttaaag catcgtgtag tgtttttctt agtccgttac gtaggtagga atctgatgta
     3961 atggttgttg gtattttgtc accattcatt tttatctggt tgttctcaag ttcggttacg
     4021 agatccattt gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg gcggcctcgc
     4081 ttatcaacca ccaatttcat attgctgtaa gtgtttaaat ctttacttat tggtttcaaa
     4141 acccattggt taagcctttt aaactcatgg tagttatttt caagcattaa catgaactta
     4201 aattcatcaa ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt tagttctttt
     4261 aataaccact cataaatcct catagagtat ttgttttcaa aagacttaac atgttccaga
     4321 ttatatttta tgaatttttt taactggaaa agataaggca atatctcttc actaaaaact
     4381 aattctaatt tttcgcttga gaacttggca tagtttgtcc actggaaaat ctcaaagcct
     4441 ttaaccaaag gattcctgat ttccacagtt ctcgtcatca gctctctggt tgctttagct
     4501 aatacaccat aagcattttc cctactgatg ttcatcatct gagcgtattg gttataagtg
     4561 aacgataccg tccgttcttt ccttgtaggg ttttcaatcg tggggttgag tagtgccaca
     4621 cagcataaaa ttagcttggt ttcatgctcc gttaagtcat agcgactaat cgctagttca
     4681 tttgctttga aaacaactaa ttcagacata catctcaatt ggtctaggtg attttaatca
     4741 ctataccaat tgagatgggc tagtcaatga taattactag tccttttccc gggagatctg
     4801 ggtatctgta aattctgcta gacctttgct ggaaaacttg taaattctgc tagaccctct
     4861 gtaaattccg ctagaccttt gtgtgttttt tttgtttata ttcaagtggt tataatttat
     4921 agaataaaga aagaataaaa aaagataaaa agaatagatc ccagccctgt gtataactca
     4981 ctactttagt cagttccgca gtattacaaa aggatgtcgc aaacgctgtt tgctcctcta
     5041 caaaacagac cttaaaaccc taaaggctta agtagcaccc tcgcaagctc gggcaaatcg
     5101 ctgaatattc cttttgtctc cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat
     5161 tcagttcgct gcgctcacgg ctctggcagt gaatgggggt aaatggcact acaggcgcct
     5221 tttatggatt catgcaagga aactacccat aatacaagaa aagcccgtca cgggcttctc
     5281 agggcgtttt atggcgggtc tgctatgtgg tgctatctga ctttttgctg ttcagcagtt
     5341 cctgccctct gattttccag tctgaccact tcggattatc ccgtgacagg tcattcagac
     5401 tggctaatgc acccagtaag gcagcggtat catcaacagg cttacccgtc ttactgtccc
     5461 tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     5521 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     5581 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     5641 ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc
     5701 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     5761 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     5821 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     5881 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     5941 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     6001 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga
     6061 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     6121 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     6181 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     6241 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     6301 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt
     6361 cgccagatat cgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     6421 gtatcacgag gccctttcgt cttcac
//
