LOCUS       40924_20696        3230 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter gene-fusion vector pFU95, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Uliczka F, Pisano F, Kochut A, Opitz W, Herbst K, Stolz T, Dersch P.
  TITLE     Monitoring of Gene Expression in Bacteria during Infections Using an
            Adaptable Set of Bioluminescent, Fluorescent and Colorigenic Fusion 
            Vectors
  JOURNAL   PLoS ONE 6 (6), E20425 (2011)
  PUBMED    21673990
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Uliczka F.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2011) Department of Molecular Infection Biology, 
            Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 
            Braunschweig, Niedersachsen 38124, Germany
REFERENCE   3  (bases 1 to 3230)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3230)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2011"; volume: "6"; issue: "6"; pages: "E20425"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-APR-2011) Department of Molecular Infection Biology, Helmholtz 
            Centre for Infection Research, Inhoffenstrasse 7, Braunschweig, 
            Niedersachsen 38124, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      12..357
                     /label=derived from Escherichia coli gapA
                     /note="derived from Escherichia coli gapA"
                     /regulatory_class="promoter"
     regulatory      365..370
                     /gene="gfpmut3.1"
                     /regulatory_class="ribosome_binding_site"
     CDS             379..1092
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     terminator      1115..1201
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(1365..1953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(2041..2135)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2168..3025)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3026..3130)
                     /label=AmpR promoter
ORIGIN
        1 ctcgaggatc cgccatttgc tcacatctca ctttaatcgt gctcacatta cgtgactgat
       61 tctaacaaaa cattaacacc aactggcaaa attttgtcct aaacttgatc tcgacgaaat
      121 ggctgcacct aaatcgtgat gaaaatcaca tttttatcgt aattgccctt taaaattcgg
      181 ggcgccgacc ccatgtggtc tcaagcccaa aggaagagtg aggcgagtca gtcgcgtaat
      241 gcttaggcac aggattgatt tgtcgcaatg attgacacga ttccgcttga cgctgcgtaa
      301 ggtttttgta attttacagg caacctttta ttcactaaca aatagctggt ggaatatgtc
      361 gacgaggaga aattaagcat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
      421 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      481 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      541 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      601 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      661 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      721 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      781 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      841 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      901 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      961 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     1021 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     1081 gaactataca aataagcggc cgctctagag gcatcaaata aaacgaaagg ctcagtcgaa
     1141 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     1201 tccgccgccc tagacctagg cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1261 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1321 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1381 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1441 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1501 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     1561 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1621 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1681 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1741 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1801 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1861 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1921 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1981 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgac tagtgcttgg
     2041 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     2101 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgtaa acttggtctg
     2161 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2221 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2281 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2341 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2401 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2461 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2521 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2581 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2641 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2701 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2761 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2821 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2881 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2941 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3001 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3061 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3121 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     3181 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac
//
