LOCUS       V006158                 5007 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006158
VERSION     V006158
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Uliczka F, Pisano F, Kochut A, Opitz W, Herbst K, Stolz T, Dersch P.
  TITLE     Monitoring of Gene Expression in Bacteria during Infections Using an
            Adaptable Set of Bioluminescent, Fluorescent and Colorigenic Fusion
            Vectors
  JOURNAL   PLoS ONE 6 (6), E20425 (2011)
   PUBMED   21673990
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Uliczka F.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2011) Department of Molecular Infection Biology,
            Helmholtz Centre for Infection Research, Inhoffenstrasse 7,
            Braunschweig, Niedersachsen 38124, Germany
REFERENCE   3  (bases 1 to 5007)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5007)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2011"; volume: "6"; issue: "6"; pages: "E20425"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-APR-2011) Department of Molecular Infection Biology, Helmholtz
            Centre for Infection Research, Inhoffenstrasse 7, Braunschweig,
            Niedersachsen 38124, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      36..42
                     /gene="phoA"
                     /regulatory_class="ribosome_binding_site"
     CDS             50..1462
                     /gene="phoA"
                     /label="Alkaline phosphatase"
                     /note="Alkaline phosphatase from Escherichia coli (strain
                     K12). Accession#: P00634"
     terminator      1485..1571
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(2109..3056)
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     rep_origin      complement(3104..3326)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3818..3912)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3945..4802)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4803..4907)
                     /label="AmpR promoter"
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacaggag gaattaacca tgaaacaaag
       61 cactattgca ctggcactct taccgttact gtttacccct gtgacaaaag cccggacacc
      121 agaaatgcct gttctggaaa accgggctgc tcagggcgat attactgcac ccggcggtgc
      181 tcgccgttta acgggtgatc agactgccgc tctgcgtgat tctcttagcg ataaacctgc
      241 aaaaaatatt attttgctga ttggcgatgg gatgggggac tcggaaatta ctgccgcacg
      301 taattatgcc gaaggtgcgg gcggcttttt taaaggtata gatgccttac cgcttaccgg
      361 gcaatacact cactatgcgc tgaataaaaa aaccggcaaa ccggactacg tcaccgactc
      421 ggctgcatca gcaaccgcct ggtcaaccgg tgtcaaaacc tataacggcg cgctgggcgt
      481 cgatattcac gaaaaagatc acccaacgat tctggaaatg gcaaaagccg caggtctggc
      541 gaccggtaac gtttctaccg cagagttgca ggatgccacg cccgctgcgc tggtggcaca
      601 tgtgacctcg cgcaaatgct acggtccgag cgcgaccagt gaaaaatgtc cgggtaacgc
      661 tctggaaaaa ggcggaaaag gatcgattac cgaacagctg cttaacgctc gtgccgacgt
      721 tacgcttggc ggcggcgcaa aaacctttgc tgaaacggca accgctggtg aatggcaggg
      781 aaaaacgctg cgtgaacagg cacaggcgcg tggttatcag ttggtgagcg atgctgcctc
      841 actgaattcg gtgacggaag cgaatcagca aaaacccctg cttggcctgt ttgctgacgg
      901 caatatgcca gtgcgctggc taggaccgaa agcaacgtac catggcaata tcgataagcc
      961 cgcagtcacc tgtacgccaa atccgcaacg taatgacagt gtaccaaccc tggcgcagat
     1021 gaccgacaaa gccattgaat tgttgagtaa aaatgagaaa ggctttttcc tgcaagttga
     1081 aggtgcgtca atcgataaac aggatcatgc tgcgaatcct tgtgggcaaa ttggcgagac
     1141 ggtcgatctc gatgaagccg tacaacgggc gctggaattc gctaaaaagg agggtaacac
     1201 gctggtcata gtcaccgctg atcacgccca cgccagccag attgttgcgc cggataccaa
     1261 agctccgggc ctcacccagg cgctaaatac caaagatggc gcagtgatgg tgatgagtta
     1321 cgggaactcc gaagaggatt cacaagaaca taccggcagt cagttgcgta ttgcggcgta
     1381 tggcccgcat gccgccaatg ttgttggact gaccgaccag accgatctct tctacaccat
     1441 gaaagccgct ctggggctga aataagcggc cgctctagag gcatcaaata aaacgaaagg
     1501 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     1561 gtaggacaaa tccgccgccc tagacctagg gtacgggttt tgctgcccgc aaacgggctg
     1621 ttctggtgtt gctagtttgt tatcagaatc gcagatccgg cttcaggttt gccggctgaa
     1681 agcgctattt cttccagaat tgccatgatt ttttccccac gggaggcgtc actggctccc
     1741 gtgttgtcgg cagctttgat tcgataagca gcatcgcctg tttcaggctg tctatgtgtg
     1801 actgttgagc tgtaacaagt tgtctcaggt gttcaatttc atgttctagt tgctttgttt
     1861 tactggtttc acctgttcta ttaggtgtta catgctgttc atctgttaca ttgtcgatct
     1921 gttcatggtg aacagcttta aatgcaccaa aaactcgtaa aagctctgat gtatctatct
     1981 tttttacacc gttttcatct gtgcatatgg acagttttcc ctttgatatc taacggtgaa
     2041 cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca agagccataa
     2101 gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg ttgtttttgc
     2161 gtgagccatg agaacgaacc attgagatca tgcttacttt gcatgtcact caaaaatttt
     2221 gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt ttttcttagt
     2281 ccgttacgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc attcattttt
     2341 atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc aacttggaaa
     2401 atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt gctgtaagtg
     2461 tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa ctcatggtag
     2521 ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat atttgccttg
     2581 tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat agagtatttg
     2641 ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa ctggaaaaga
     2701 taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa cttggcatag
     2761 tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc cacagttctc
     2821 gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct actgatgttc
     2881 atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct tgtagggttt
     2941 tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc atgctccgtt
     3001 aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc agacatacat
     3061 ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag tcaatgataa
     3121 ttactagtcc ttttcccggg agatctgggt atctgtaaat tctgctagac ctttgctgga
     3181 aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg tgtttttttt
     3241 gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa gataaaaaga
     3301 atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta ttacaaaagg
     3361 atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa aggcttaagt
     3421 agcaccctcg caagctcggg caaatcgctg aatattcctt ttgtctccga ccatcaggca
     3481 cctgagtcgc tgtctttttc gtgacattca gttcgctgcg ctcacggctc tggcagtgaa
     3541 tgggggtaaa tggcactaca ggcgcctttt atggattcat gcaaggaaac tacccataat
     3601 acaagaaaag cccgtcacgg gcttctcagg gcgttttatg gcgggtctgc tatgtggtgc
     3661 tatctgactt tttgctgttc agcagttcct gccctctgat tttccagtct gaccacttcg
     3721 gattatcccg tgacaggtca ttcagactgg ctaatgcacc cagtaaggca gcggtatcat
     3781 caacaggctt acccgtctta ctgtccctag tgcttggatt ctcaccaata aaaaacgccc
     3841 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     3901 tcaacaggag tccaagcgag ctcgtaaact tggtctgaca gttaccaatg cttaatcagt
     3961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     4381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     4441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     4561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     4921 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     4981 cgtatcacga ggccctttcg tcttcac
//
