LOCUS       V006159                 4993 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006159
VERSION     V006159
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Uliczka F, Pisano F, Kochut A, Opitz W, Herbst K, Stolz T, Dersch P.
  TITLE     Monitoring of Gene Expression in Bacteria during Infections Using an
            Adaptable Set of Bioluminescent, Fluorescent and Colorigenic Fusion
            Vectors
  JOURNAL   PLoS ONE 6 (6), E20425 (2011)
   PUBMED   21673990
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Uliczka F.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2011) Department of Molecular Infection Biology,
            Helmholtz Centre for Infection Research, Inhoffenstrasse 7,
            Braunschweig, Niedersachsen 38124, Germany
REFERENCE   3  (bases 1 to 4993)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4993)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2011"; volume: "6"; issue: "6"; pages: "E20425"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-APR-2011) Department of Molecular Infection Biology, Helmholtz
            Centre for Infection Research, Inhoffenstrasse 7, Braunschweig,
            Niedersachsen 38124, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             36..1448
                     /gene="phoA"
                     /label="Alkaline phosphatase"
                     /note="Alkaline phosphatase from Escherichia coli (strain
                     K12). Accession#: P00634"
     terminator      1471..1557
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(2095..3042)
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     rep_origin      complement(3090..3312)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3804..3898)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3931..4788)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4789..4893)
                     /label="AmpR promoter"
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacatgaa acaaagcact attgcactgg
       61 cactcttacc gttactgttt acccctgtga caaaagcccg gacaccagaa atgcctgttc
      121 tggaaaaccg ggctgctcag ggcgatatta ctgcacccgg cggtgctcgc cgtttaacgg
      181 gtgatcagac tgccgctctg cgtgattctc ttagcgataa acctgcaaaa aatattattt
      241 tgctgattgg cgatgggatg ggggactcgg aaattactgc cgcacgtaat tatgccgaag
      301 gtgcgggcgg cttttttaaa ggtatagatg ccttaccgct taccgggcaa tacactcact
      361 atgcgctgaa taaaaaaacc ggcaaaccgg actacgtcac cgactcggct gcatcagcaa
      421 ccgcctggtc aaccggtgtc aaaacctata acggcgcgct gggcgtcgat attcacgaaa
      481 aagatcaccc aacgattctg gaaatggcaa aagccgcagg tctggcgacc ggtaacgttt
      541 ctaccgcaga gttgcaggat gccacgcccg ctgcgctggt ggcacatgtg acctcgcgca
      601 aatgctacgg tccgagcgcg accagtgaaa aatgtccggg taacgctctg gaaaaaggcg
      661 gaaaaggatc gattaccgaa cagctgctta acgctcgtgc cgacgttacg cttggcggcg
      721 gcgcaaaaac ctttgctgaa acggcaaccg ctggtgaatg gcagggaaaa acgctgcgtg
      781 aacaggcaca ggcgcgtggt tatcagttgg tgagcgatgc tgcctcactg aattcggtga
      841 cggaagcgaa tcagcaaaaa cccctgcttg gcctgtttgc tgacggcaat atgccagtgc
      901 gctggctagg accgaaagca acgtaccatg gcaatatcga taagcccgca gtcacctgta
      961 cgccaaatcc gcaacgtaat gacagtgtac caaccctggc gcagatgacc gacaaagcca
     1021 ttgaattgtt gagtaaaaat gagaaaggct ttttcctgca agttgaaggt gcgtcaatcg
     1081 ataaacagga tcatgctgcg aatccttgtg ggcaaattgg cgagacggtc gatctcgatg
     1141 aagccgtaca acgggcgctg gaattcgcta aaaaggaggg taacacgctg gtcatagtca
     1201 ccgctgatca cgcccacgcc agccagattg ttgcgccgga taccaaagct ccgggcctca
     1261 cccaggcgct aaataccaaa gatggcgcag tgatggtgat gagttacggg aactccgaag
     1321 aggattcaca agaacatacc ggcagtcagt tgcgtattgc ggcgtatggc ccgcatgccg
     1381 ccaatgttgt tggactgacc gaccagaccg atctcttcta caccatgaaa gccgctctgg
     1441 ggctgaaata agcggccgct ctagaggcat caaataaaac gaaaggctca gtcgaaagac
     1501 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     1561 ccgccctaga cctagggtac gggttttgct gcccgcaaac gggctgttct ggtgttgcta
     1621 gtttgttatc agaatcgcag atccggcttc aggtttgccg gctgaaagcg ctatttcttc
     1681 cagaattgcc atgatttttt ccccacggga ggcgtcactg gctcccgtgt tgtcggcagc
     1741 tttgattcga taagcagcat cgcctgtttc aggctgtcta tgtgtgactg ttgagctgta
     1801 acaagttgtc tcaggtgttc aatttcatgt tctagttgct ttgttttact ggtttcacct
     1861 gttctattag gtgttacatg ctgttcatct gttacattgt cgatctgttc atggtgaaca
     1921 gctttaaatg caccaaaaac tcgtaaaagc tctgatgtat ctatcttttt tacaccgttt
     1981 tcatctgtgc atatggacag ttttcccttt gatatctaac ggtgaacagt tgttctactt
     2041 ttgtttgtta gtcttgatgc ttcactgata gatacaagag ccataagaac ctcagatcct
     2101 tccgtattta gccagtatgt tctctagtgt ggttcgttgt ttttgcgtga gccatgagaa
     2161 cgaaccattg agatcatgct tactttgcat gtcactcaaa aattttgcct caaaactggt
     2221 gagctgaatt tttgcagtta aagcatcgtg tagtgttttt cttagtccgt tacgtaggta
     2281 ggaatctgat gtaatggttg ttggtatttt gtcaccattc atttttatct ggttgttctc
     2341 aagttcggtt acgagatcca tttgtctatc tagttcaact tggaaaatca acgtatcagt
     2401 cgggcggcct cgcttatcaa ccaccaattt catattgctg taagtgttta aatctttact
     2461 tattggtttc aaaacccatt ggttaagcct tttaaactca tggtagttat tttcaagcat
     2521 taacatgaac ttaaattcat caaggctaat ctctatattt gccttgtgag ttttcttttg
     2581 tgttagttct tttaataacc actcataaat cctcatagag tatttgtttt caaaagactt
     2641 aacatgttcc agattatatt ttatgaattt ttttaactgg aaaagataag gcaatatctc
     2701 ttcactaaaa actaattcta atttttcgct tgagaacttg gcatagtttg tccactggaa
     2761 aatctcaaag cctttaacca aaggattcct gatttccaca gttctcgtca tcagctctct
     2821 ggttgcttta gctaatacac cataagcatt ttccctactg atgttcatca tctgagcgta
     2881 ttggttataa gtgaacgata ccgtccgttc tttccttgta gggttttcaa tcgtggggtt
     2941 gagtagtgcc acacagcata aaattagctt ggtttcatgc tccgttaagt catagcgact
     3001 aatcgctagt tcatttgctt tgaaaacaac taattcagac atacatctca attggtctag
     3061 gtgattttaa tcactatacc aattgagatg ggctagtcaa tgataattac tagtcctttt
     3121 cccgggagat ctgggtatct gtaaattctg ctagaccttt gctggaaaac ttgtaaattc
     3181 tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt ttttttgttt atattcaagt
     3241 ggttataatt tatagaataa agaaagaata aaaaaagata aaaagaatag atcccagccc
     3301 tgtgtataac tcactacttt agtcagttcc gcagtattac aaaaggatgt cgcaaacgct
     3361 gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc ttaagtagca ccctcgcaag
     3421 ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat caggcacctg agtcgctgtc
     3481 tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg ggtaaatggc
     3541 actacaggcg ccttttatgg attcatgcaa ggaaactacc cataatacaa gaaaagcccg
     3601 tcacgggctt ctcagggcgt tttatggcgg gtctgctatg tggtgctatc tgactttttg
     3661 ctgttcagca gttcctgccc tctgattttc cagtctgacc acttcggatt atcccgtgac
     3721 aggtcattca gactggctaa tgcacccagt aaggcagcgg tatcatcaac aggcttaccc
     3781 gtcttactgt ccctagtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc
     3841 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
     3901 agcgagctcg taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3961 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4021 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4081 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4141 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4201 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4261 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4321 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4381 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4441 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4501 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4561 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4621 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4681 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4741 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4801 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4861 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4921 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     4981 ctttcgtctt cac
//
