LOCUS       40924_20681        9361 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter gene-fusion vector pFU72, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Uliczka F, Pisano F, Kochut A, Opitz W, Herbst K, Stolz T, Dersch P.
  TITLE     Monitoring of Gene Expression in Bacteria during Infections Using an
            Adaptable Set of Bioluminescent, Fluorescent and Colorigenic Fusion 
            Vectors
  JOURNAL   PLoS ONE 6 (6), E20425 (2011)
  PUBMED    21673990
REFERENCE   2  (bases 1 to 9361)
  AUTHORS   Uliczka F.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2011) Department of Molecular Infection Biology, 
            Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 
            Braunschweig, Niedersachsen 38124, Germany
REFERENCE   3  (bases 1 to 9361)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9361)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2011"; volume: "6"; issue: "6"; pages: "E20425"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-APR-2011) Department of Molecular Infection Biology, Helmholtz 
            Centre for Infection Research, Inhoffenstrasse 7, Braunschweig, 
            Niedersachsen 38124, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             36..1475
                     /codon_start=1
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             1491..2411
                     /codon_start=1
                     /label=LuxD
                     /note="LuxD acyltransferase"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2463..3542
                     /codon_start=1
                     /label=LuxA
                     /note="LuxA luciferase subunit"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3560..4540
                     /codon_start=1
                     /label=LuxB
                     /note="LuxB luciferase subunit"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             4722..5831
                     /codon_start=1
                     /label=LuxE
                     /note="LuxE"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     terminator      5854..5940
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    complement(5966..5982)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      complement(6017..6405)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(7026..7394)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(7427..7536)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     terminator      complement(8172..8266)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(8299..9156)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9157..9261)
                     /label=AmpR promoter
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacatgac taaaaaaatt tcattcatta
       61 ttaacggcca ggttgaaatc tttcccgaag gtgatgattt agtgcaatcc attaattttg
      121 gtgataatag tgtttacctg ccaatattga atgactctca tgtaaaaaac attattgatt
      181 gtaatggaaa taacgaatta cggttgcata acattgtcaa ttttctctat acggtagggc
      241 aaagatggaa aaatgaagaa tactcaagac gcaggacata cattcgtgac ttaaaaaaat
      301 atatgggata ttcagaagaa atggctaagc tagaggccaa ttggatatct atgattttat
      361 gttctaaagg cggcctttat gatgttgtag aaaatgaact tggttctcgc catatcatgg
      421 atgaatggct acctcaggat gaaagttatg ttcgggcttt tccgaaaggt aaatctgtac
      481 atctgttggc aggtaatgtt ccattatctg ggatcatgtc tatattacgc gcaattttaa
      541 ctaagaatca gtgtattata aaaacatcgt caaccgatcc ttttaccgct aatgcattag
      601 cgttaagttt tattgatgta gaccctaatc atccgataac gcgctcttta tctgttatat
      661 attggcccca ccaaggtgat acatcactcg caaaagaaat tatgcaacat gcggatgtta
      721 ttgtcgcttg gggagggcca gatgcgatta attgggcggt agagcatgcg ccatcttatg
      781 ctgatgtgat taaatttggt tctaaaaaga gtctttgcat tatcgataat cctgttgatt
      841 tgacgtccgc agcgacaggt gcggctcatg atgtttgttt ttacgatcag cgagcttgtt
      901 tttctgccca aaacatatat tacatgggaa atcattatga ggaatttaag ttagcgttga
      961 tagaaaaact taatctatat gcgcatatat taccgaatgc caaaaaagat tttgatgaaa
     1021 aggcggccta ttctttagtt caaaaagaaa gcttgtttgc tggattaaaa gtagaggtgg
     1081 atattcatca acgttggatg attattgagt caaatgcagg tgtggaattt aatcaaccac
     1141 ttggcagatg tgtgtacctt catcacgtcg ataatattga gcaaatattg ccttatgttc
     1201 aaaaaaataa gacgcaaacc atatctattt ttccttggga gtcatcattt aaatatcgag
     1261 atgcgttagc attaaaaggt gcggaaagga ttgtagaagc aggaatgaat aacatatttc
     1321 gagttggtgg atctcatgac ggaatgagac cgttgcaacg attagtgaca tatatttctc
     1381 atgaaaggcc atctaactat acggctaagg atgttgcggt tgaaatagaa cagactcgat
     1441 tcctggaaga agataagttc cttgtatttg tcccataata ggtaaaaagt atggaaaatg
     1501 aatcaaaata taaaaccatc gaccacgtta tttgtgttga aggaaataaa aaaattcatg
     1561 tttgggaaac gctgccagaa gaaaacagcc caaagagaaa gaatgccatt attattgcgt
     1621 ctggttttgc ccgcaggatg gatcattttg ctggtctggc ggaatattta tcgcggaatg
     1681 gatttcatgt gatccgctat gattcgcttc accacgttgg attgagttca gggacaattg
     1741 atgaatttac aatgtctata ggaaagcaga gcttgttagc agtggttgat tggttaacta
     1801 cacgaaaaat aaataacttc ggtatgttgg cttcaagctt atctgcgcgg atagcttatg
     1861 caagcctatc tgaaatcaat gcttcgtttt taatcaccgc agtcggtgtt gttaacttaa
     1921 gatattctct tgaaagagct ttagggtttg attatctcag tctacccatt aatgaattgc
     1981 cgaataatct agattttgaa ggccataaat tgggtgctga agtctttgcg agagattgtc
     2041 ttgattttgg ttgggaagat ttagcttcta caattaataa catgatgtat cttgatatac
     2101 cgtttattgc ttttactgca aataacgata attgggtcaa gcaagatgaa gttatcacat
     2161 tgttatcaaa tattcgtagt aatcgatgca agatatattc tttgttagga agttcgcatg
     2221 acttgagtga aaatttagtg gtcctgcgca atttttatca atcggttacg aaagccgcta
     2281 tcgcgatgga taatgatcat ctggatattg atgttgatat tactgaaccg tcatttgaac
     2341 atttaactat tgcgacagtc aatgaacgcc gaatgagaat tgagattgaa aatcaagcaa
     2401 tttctctgtc ttaaaatcta ttgagatatt ctatcactca aatagcaata taaggactct
     2461 ctatgaaatt tggaaacttt ttgcttacat accaacctcc ccaattttct caaacagagg
     2521 taatgaaacg tttggttaaa ttaggtcgca tctctgagga gtgtggtttt gataccgtat
     2581 ggttactgga gcatcatttc acggagtttg gtttgcttgg taacccttat gtcgctgctg
     2641 catatttact tggcgcgact aaaaaattga atgtaggaac tgccgctatt gttcttccca
     2701 cagcccatcc agtacgccaa cttgaagatg tgaatttatt ggatcaaatg tcaaaaggac
     2761 gatttcggtt tggtatttgc cgagggcttt acaacaagga ctttcgcgta ttcggcacag
     2821 atatgaataa cagtcgcgcc ttagcggaat gctggtacgg gctgataaag aatggcatga
     2881 cagagggata tatggaagct gataatgaac atatcaagtt ccataaggta aaagtaaacc
     2941 ccgcggcgta tagcagaggt ggcgcaccgg tttatgtggt ggctgaatca gcttcgacga
     3001 ctgagtgggc tgctcaattt ggcctaccga tgatattaag ttggattata aatactaacg
     3061 aaaagaaagc acaacttgag ctttataatg aagtggctca agaatatggg cacgatattc
     3121 ataatatcga ccattgctta tcatatataa catctgtaga tcatgactca attaaagcga
     3181 aagagatttg ccggaaattt ctggggcatt ggtatgattc ttatgtgaat gctacgacta
     3241 tttttgatga ttcagaccaa acaagaggtt atgatttcaa taaagggcag tggcgtgact
     3301 ttgtattaaa aggacataaa gatactaatc gccgtattga ttacagttac gaaatcaatc
     3361 ccgtgggaac gccgcaggaa tgtattgaca taattcaaaa agacattgat gctacaggaa
     3421 tatcaaatat ttgttgtgga tttgaagcta atggaacagt agacgaaatt attgcttcca
     3481 tgaagctctt ccagtctgat gtcatgccat ttcttaaaga aaaacaacgt tcgctattat
     3541 attagctaag gagaaagaaa tgaaatttgg attgttcttc cttaacttca tcaattcaac
     3601 aactgttcaa gaacaaagta tagttcgcat gcaggaaata acggagtatg ttgataagtt
     3661 gaattttgaa cagattttag tgtatgaaaa tcatttttca gataatggtg ttgtcggcgc
     3721 tcctctgact gtttctggtt ttctgctcgg tttaacagag aaaattaaaa ttggttcatt
     3781 aaatcacatc attacaactc atcatcctgt ccgcatagcg gaggaagctt gcttattgga
     3841 tcagttaagt gaagggagat ttattttagg gtttagtgat tgcgaaaaaa aagatgaaat
     3901 gcattttttt aatcgcccgg ttgaatatca acagcaacta tttgaagagt gttatgaaat
     3961 cattaacgat gctttaacaa caggctattg taatccagat aacgattttt atagcttccc
     4021 taaaatatct gtaaatcccc atgcttatac gccaggcgga cctcggaaat atgtaacagc
     4081 aaccagtcat catattgttg agtgggcggc caaaaaaggt attcctctca tctttaagtg
     4141 ggatgattct aatgatgtta gatatgaata tgctgaaaga tataaagccg ttgcggataa
     4201 atatgacgtt gacctatcag agatagacca tcagttaatg atattagtta actataacga
     4261 agatagtaat aaagctaaac aagagacgcg tgcatttatt agtgattatg ttcttgaaat
     4321 gcaccctaat gaaaatttcg aaaataaact tgaagaaata attgcagaaa acgctgtcgg
     4381 aaattatacg gagtgtataa ctgcggctaa gttggcaatt gaaaagtgtg gtgcgaaaag
     4441 tgtattgctg tcctttgaac caatgaatga tttgatgagc caaaaaaatg taatcaatat
     4501 tgttgatgat aatattaaga agtaccacat ggaatatacc taatagattt cgagttgcag
     4561 cgaggcggca agtgaacgaa tccccaggag catagataac tatgtgactg gggtgagtga
     4621 aagcagccaa caaagcagca gcttgaaaga tgaagggtat aaaagagtat gacagcagtg
     4681 ctgccatact ttctaatatt atcttgagga gtaaaacagg tatgacttca tatgttgata
     4741 aacaagaaat tacagcaagc tcagaaattg atgatttgat tttttcgagc gatccattag
     4801 tgtggtctta cgacgagcag gaaaaaatca gaaagaaact tgtgcttgat gcatttcgta
     4861 atcattataa acattgtcga gaatatcgtc actactgtca ggcacacaaa gtagatgaca
     4921 atattacgga aattgatgac atacctgtat tcccaacatc ggtttttaag tttactcgct
     4981 tattaacttc tcaggaaaac gagattgaaa gttggtttac cagtagcggc acgaatggtt
     5041 taaaaagtca ggtggcgcgt gacagattaa gtattgagag actcttaggc tctgtgagtt
     5101 atggcatgaa atatgttggt agttggtttg atcatcaaat agaattagtc aatttgggac
     5161 cagatagatt taatgctcat aatatttggt ttaaatatgt tatgagtttg gtggaattgt
     5221 tatatcctac gacatttacc gtaacagaag aacgaataga ttttgttaaa acattgaata
     5281 gtcttgaacg aataaaaaat caagggaaag atctttgtct tattggttcg ccatacttta
     5341 tttatttact ctgccattat atgaaagata aaaaaatctc attttctgga gataaaagcc
     5401 tttatatcat aaccggaggc ggctggaaaa gttacgaaaa agaatctctg aaacgtgatg
     5461 atttcaatca tcttttattt gatactttca atctcagtga tattagtcag atccgagata
     5521 tatttaatca agttgaactc aacacttgtt tctttgagga tgaaatgcag cgtaaacatg
     5581 ttccgccgtg ggtatatgcg cgagcgcttg atcctgaaac gttgaaacct gtacctgatg
     5641 gaacgccggg gttgatgagt tatatggatg cgtcagcaac cagttatcca gcatttattg
     5701 ttaccgatga tgtcgggata attagcagag aatatggtaa gtatcccggc gtgctcgttg
     5761 aaattttacg tcgcgtcaat acgaggacgc agaaagggtg tgctttaagc ttaaccgaag
     5821 cgtttgatag ttgagcggcc gctctagagg catcaaataa aacgaaaggc tcagtcgaaa
     5881 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     5941 ccgccgccct agacctaggc acaaattgtt atccgctcac aattccacat gtggaattcc
     6001 acatgtggaa ttcccatgtc agccgttaag tgttcctgtg tcactcaaaa ttgctttgag
     6061 aggctctaag ggcttctcag tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc
     6121 ttaaaagctt taaaagcctt atatattctt ttttttctta taaaacttaa aaccttagag
     6181 gctatttaag ttgctgattt atattaattt tattgttcaa acatgagagc ttagtacgtg
     6241 aaacatgaga gcttagtacg ttagccatga gagcttagta cgttagccat gagggtttag
     6301 ttcgttaaac atgagagctt agtacgttaa acatgagagc ttagtacgtg aaacatgaga
     6361 gcttagtacg tactatcaac aggttgaact gctgatcttc agatcctcta cgccggacgc
     6421 atcgtggccg gatccagccg accaggcttt ccacgcccgc gtgccgctcc atgtcgttcg
     6481 cgcggttctc ggaaacgcgc tgccgcgttt cgtgattgtc acgctcaagc ccgtagtccc
     6541 gttcgagcgt cgcgcagagg tcagcgaggg cgcggtaggc ccgatacggc tcatggatgg
     6601 tgtttcgggt cgggtgaatc ttgttgatgg cgatatggat gtgcaggttg tcggtgtcgt
     6661 gatgcacggc actgacgcgc tgatgctcgg cgaagccaag cccagcgcag atgcggtcct
     6721 caatcgcgcg caacgtctcc gcgtcgggct tctctcccgc gcggaagcta accagcaggt
     6781 gataggtctt gtcggcctcg gaacgggtgt tgccgtgctg ggtcgccatc acctcggcca
     6841 tgacagcggg cagggtgttt gcctcgcagt tcgtgacgcg cacgtgaccc aggcgctcgg
     6901 tcttgccttg ctcgtcggtg atgtacttca ccagctccgc gaagtcgctc ttcttgatgg
     6961 agcgcatggg gacgtgcttg gcaatcacgc gcaccccccg gccgttttag cggctaaaaa
     7021 agtcatggct ctgccctcgg gcggaccacg cccatcatga ccttgccaag ctcgtcctgc
     7081 ttctcttcga tcttcgccag cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc
     7141 gggtcgtcgg tgagccagag tttcagcagg ccgcccaggc ggcccaggtc gccattgatg
     7201 cgggccagct cgcggacgtg ctcatagtcc acgacgcccg tgattttgta gccctggccg
     7261 acggccagca ggtaggccga caggctcatg ccggccgccg ccgccttttc ctcaatcgct
     7321 cttcgttcgt ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttggcttg
     7381 gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc
     7441 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
     7501 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
     7561 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
     7621 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa gcgctgcttc
     7681 cctgctgttt tgtggaatat ctaccgactg gaaacaggca aatgcaggaa attactgaac
     7741 tgaggggaca ggcgagagac gatgccaaag agctacaccg acgagctggc cgagtgggtt
     7801 gaatcccgcg cggccaagaa gcgccggcgt gatgaggctg cggttgcgtt cctggcggtg
     7861 agggcggatg tcgaggcggc gttagcgtcc ggctatgcgc tcgtcaccat ttgggagcac
     7921 atgcgggaaa cggggaaggt caagttctcc tacgagacgt tccgctcgca cgccaggcgg
     7981 cacatcaagg ccaagcccgc cgatgtgccc gcaccgcagg ccaaggctgc ggaacccgcg
     8041 ccggcaccca agacgccgga gccacggcgg ccgaagcagg ggggcaaggc tgaaaagccg
     8101 gcccccgctg cggccccgac cggcttcacc ttcaacccaa caccggacaa aaaggatcca
     8161 ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     8221 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgta
     8281 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8341 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8401 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8461 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8521 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8581 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8641 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8701 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8761 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8821 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8881 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8941 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9001 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9061 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9121 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9181 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9241 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     9301 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     9361 c
//
