LOCUS       40924_20676        5253 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter gene-fusion vector pFU62, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Uliczka F, Pisano F, Kochut A, Opitz W, Herbst K, Stolz T, Dersch P.
  TITLE     Monitoring of Gene Expression in Bacteria during Infections Using an
            Adaptable Set of Bioluminescent, Fluorescent and Colorigenic Fusion 
            Vectors
  JOURNAL   PLoS ONE 6 (6), E20425 (2011)
  PUBMED    21673990
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Uliczka F.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2011) Department of Molecular Infection Biology, 
            Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 
            Braunschweig, Niedersachsen 38124, Germany
REFERENCE   3  (bases 1 to 5253)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5253)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2011"; volume: "6"; issue: "6"; pages: "E20425"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-APR-2011) Department of Molecular Infection Biology, Helmholtz 
            Centre for Infection Research, Inhoffenstrasse 7, Braunschweig, 
            Niedersachsen 38124, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      36..42
                     /gene="lacZ"
                     /regulatory_class="ribosome_binding_site"
     CDS             44..3115
                     /label=lacZ
                     /note="beta-galactosidase"
     terminator      3138..3224
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(3388..3976)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(4064..4158)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4191..5048)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5049..5153)
                     /label=AmpR promoter
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacaggag gaattaaaaa tgaaagggaa
       61 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      121 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      181 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc
      241 accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt
      301 cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta
      361 tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct
      421 cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg
      481 cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag
      541 tcgtttgccg tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc
      601 ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa gatcaggata tgtggcggat
      661 gagcggcatt ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt
      721 ccatgttgcc actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca
      781 gatgtgcggc gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac
      841 gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta
      901 tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat
      961 cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc
     1021 agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa
     1081 cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca
     1141 ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa
     1201 cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta
     1261 cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg
     1321 tctgaccgat gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca
     1381 gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgaat caggccacgg
     1441 cgctaatcac gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca
     1501 gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg
     1561 cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc
     1621 gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag
     1681 tcttggcggt ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg
     1741 cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg
     1801 gtcggcttac ggcggtgatt ttggcgatac gccgaacgat cgccagttct gtatgaacgg
     1861 tctggtcttt gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac accagcagca
     1921 gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg
     1981 tcatagcgat aacgaactcc tgcactggat ggtggcgctg gatggtaagc cgctggcaag
     2041 cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac tgcctgaact
     2101 accgcagccg gagagcgccg ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc
     2161 gaccgcatgg tcagaagccg ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa
     2221 cctcagtgtg acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat
     2281 ggatttttgc atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct
     2341 ttcacagatg tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac
     2401 ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc
     2461 ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg
     2521 cacggcagat acacttgctg atgcggtgct gattacgacc gctcacgcgt ggcagcatca
     2581 ggggaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc
     2641 gattaccgtt gatgttgaag tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa
     2701 ctgccagctg gcgcaggtag cagagcgggt aaactggctc ggattagggc cgcaagaaaa
     2761 ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat
     2821 gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa
     2881 ttatggccca caccagtggc gcggcgactt ccagttcaac atcagccgct acagtcaaca
     2941 gcaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa
     3001 tatcgacggt ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtatcggc
     3061 ggaattacag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataagc
     3121 ggccgctcta gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     3181 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ccctagacct
     3241 aggcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3301 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3361 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3421 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3481 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3541 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
     3601 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3661 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3721 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3781 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     3841 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3901 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3961 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4021 cgaaaactca cgttaaggga ttttggtcat gactagtgct tggattctca ccaataaaaa
     4081 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     4141 gatctatcaa caggagtcca agcgagctcg taaacttggt ctgacagtta ccaatgctta
     4201 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4261 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4321 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4381 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4441 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4501 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4561 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4621 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4681 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4741 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4801 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4861 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4921 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4981 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5041 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5101 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     5161 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     5221 aataggcgta tcacgaggcc ctttcgtctt cac
//
